RESEARCH ARTICLE
SNAP23 depletion enables more
SNAP25/calcium channel excitosome
formation to increase insulin exocytosis
in type 2 diabetes
Tao Liang,1 Tairan Qin,1 Fei Kang,1 Youhou Kang,1 Li Xie,1 Dan Zhu,1 Subhankar Dolai,1
Dafna Greitzer-Antes,1 Robert K. Baker,2 Daorong Feng,3 Eva Tuduri,2 Claes-Goran Ostenson,4,5
Timothy J. Kieffer,2 Kate Banks,6 Jeffrey E. Pessin,3 and Herbert Y. Gaisano1
1
Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. 2Department of Cellular
and Physiological Sciences, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
3
Michael F. Price Center for Genetic and Translational Medicine, Department of Medicine and Department of Molecular
Pharmacology, Albert Einstein College of Medicine, Bronx, New York, USA. 4Department of Molecular Medicine and
5
Department of Surgery, Karolinska Institutet, Stockholm, Sweden. 6Division of Comparative Medicine, Faculty of
Medicine, University of Toronto, Toronto, Ontario, Canada.
SNAP23 is the ubiquitous SNAP25 isoform that mediates secretion in non-neuronal cells, similar to
SNAP25 in neurons. However, some secretory cells like pancreatic islet β cells contain an abundance
of both SNAP25 and SNAP23, where SNAP23 is believed to play a redundant role to SNAP25. We
show that SNAP23, when depleted in mouse β cells in vivo and human β cells (normal and type
2 diabetes [T2D] patients) in vitro, paradoxically increased biphasic glucose-stimulated insulin
secretion corresponding to increased exocytosis of predocked and newcomer insulin granules. Such
effects on T2D Goto-Kakizaki rats improved glucose homeostasis that was superior to conventional
treatment with sulfonylurea glybenclamide. SNAP23, although fusion competent in slower
secretory cells, in the context of β cells acts as a weak partial fusion agonist or inhibitory SNARE.
Here, SNAP23 depletion promotes SNAP25 to bind calcium channels more quickly and longer where
granule fusion occurs to increase exocytosis efficiency. β Cell SNAP23 antagonism is a strategy to
treat diabetes.
Introduction
Authorship note: TL, TQ, and FK
contributed equally.
Conflict of interest: The authors have
declared that no conflict of interest
exists.
Copyright: © 2020, American Society
for Clinical Investigation.
Submitted: April 19, 2019
Accepted: January 15, 2020
Published: February 13, 2020.
Reference information: JCI Insight.
2020;5(3):e129694.
https://doi.org/10.1172/jci.
insight.129694.
insight.jci.org
The membrane fusion machinery requires SNARE (soluble N-ethylmaleimide–sensitive factor attachment protein receptor) and nSec/Munc18 (SM) proteins that organize the SNARE proteins into membrane fusion–ready SNARE complexes (1). Insulin secretion from pancreatic islet β cells follows this
paradigm (2–4), employing SNARE proteins vesicle-associated membrane protein 2 and 8 (VAMP2
and -8) (5, 6), syntaxins (Stx-1, -3, and -4) (7–9), and synaptosome-associated protein of 25 kDa
(SNAP25) (10–12). Whereas these different members of the VAMP and syntaxin families have been
shown to mediate distinct exocytoses of insulin secretory granules (SGs) (reviewed in refs. 2–4), the
SNAP25 family is less well studied. SNAP23 is the ubiquitous SNAP25 isoform that mediates exocytosis in many non-neuronal cells, such as muscle and adipocytes (4, 13) and pancreatic acini (14). In
acini, SNAP23 on SGs and the plasma membrane (PM) (14) binds Stx-3 on SGs and PM-bound Stx-4
to form distinct SM/SNARE complexes that mediate different exocytotic events including apical and
basolateral exocytosis and SG-SG fusion (15).
Peculiarly, SNAP23 is abundant in endocrine β cells (16), and like SNAP25 binds all 4 exocytotic
Stxs (6, 17). SNAP25 is, however, the putative SNARE partner for all syntaxins and VAMPs in mediating
glucose-stimulated insulin secretion (GSIS) (4, 6, 17), including Stx-1 and Stx-4 with VAMP2 for exocytosis of predocked SGs (SGs already docked on the PM under basal conditions, which then fuses after
stimulation) (4, 7); and Stx-3 with VAMP8 for newcomer SGs (SGs not predocked on the PM under basal
conditions) (6, 8) and SG-SG fusion (6, 8). Newcomer SGs are divided into short-dock (short residence
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time of >200 milliseconds to a few minutes at the PM before fusion) or no-dock (<200 milliseconds at the
PM before fusion) (2, 6, 8). What, then, is the role of SNAP23 in β cells? An early study in 1997 showed
that overexpression of SNAP23 in insulinoma HIT-T15 β cells treated with botulinum toxin E that cleaves
SNAP25 but not SNAP23 could rescue GSIS (16). A similar study was reported in neuroendocrine PC12
cells (18). It was therefore long assumed that while SNAP25 plays the more dominant role in insulin secretion (10–12), SNAP23 simply plays a less important functionally redundant role to SNAP25 (16, 18). In
this work, we have found that this is not the case, at least not for β cell insulin secretion, and likely also for
other secretory cells that contain abundant amounts of both SNAP25 and SNAP23.
Here, we show that deletion of SNAP23 in vivo in β cells of mice and nonobese T2D Goto-Kakizaki
(GK) rats, and in human β cells of normal and T2D patients in vitro, resulted in paradoxically greatly
increased biphasic GSIS by increasing exocytosis of both predocked and newcomer SGs, resulting in
improved glucose homeostasis, which in GK rats was superior to glybenclamide treatment. This led us
to propose that SNAP23, although fusion competent as tested by in vitro fusion assays (19, 20), in the
context of β cells plays the role of a weak partial fusion agonist (21) or an inhibitory SNARE protein
(22), whereby SNAP23 depletion promotes more endogenous SNAP25 to form SNARE complexes with
calcium channels (called excitosomes; ref. 23) to increase exocytosis.
Results
Generation of a mouse with β cell–specific deletion of SNAP23. To assess the endogenous function of SNAP23 in β
cells, we generated SNAP23fl/fl mice (24). SNAP23 is abundant in pancreatic islet β cells and α cells, wherein
SNAP23 colocalized with insulin (Figure 1A and quantitation in Supplemental Figure 1A, bottom left; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.129694DS1) and
glucagon (Figure 1C and quantitation in Supplemental Figure 1A, bottom right). On single human (Figure
1B, first row) and mouse (Figure 1B, second row) β cells, SNAP23 seems to be more abundant in insulin SGs
and partially colocalized with Stx-1A (ref. 25; Figure 1B, third row, and quantitation in Supplemental Figure
1A, bottom middle) and SNAP25 (Figure 1B, fourth row) beneath the PM (10, 11). We then employed adeno-associated virus serotype 8–RIP1-Cre (AAV8-RIP1-Cre) in 8- to 10-week-old SNAP23fl/fl mice. This strategy has an additional advantage over conventional crossing of floxed mice to Cre mice in avoiding possible
developmental issues. We had reported that AAV8 could deliver the target gene expression into the pancreas
with high efficiency (26). The rat insulin promoter 1 (RIP1) specifically drives Cre recombinase expression in
pancreatic β cells. Two weeks after SNAP23fl/fl mice were intraperitoneally (i.p.) injected with AAV8-RIP1Cre, islets isolated from these mice showed Cre expression in β cells with cytoplasmic insulin surrounding
Cre-stained nuclei (Figure 1D, top), with no Cre expression in α cells (Figure 1D, bottom), confirming specific targeting of Cre to β cells. The resulting effective deletion of SNAP23 in β cells is shown with Cre-expressing cells devoid of SNAP23 (Figure 1E, top), and consequently the majority of insulin-positive β cells
were also devoid of SNAP23 (Figure 1E, bottom), leaving some SNAP23 staining mostly in islet periphery
cells, presumably α cells (Figure 1D, top, and Figure 1E, top; also see Figure 1C). Finally, we assessed isolated islet protein expression (Figure 1F), showing 82.1% ± 7.9% reduction in SNAP23 expression induced
by AAV8-RIP1-Cre on SNAP23fl/fl mouse islets, with residual islet SNAP23 presumably contributed largely
from α cells. Depletion of islet SNAP23 levels had no effects on other islet exocytotic proteins (Figure 1F
and analysis of n = 3 in Supplemental Figure 1B). Importantly, islet SNAP25 levels remained the same. To
verify specific targeting of SNAP23 deletion to β cells, we examined organs known to possess abundant
SNAP23 (Figure 1G and analysis of n = 3 in Supplemental Figure 1C), including whole pancreas (90%
acini), fat, muscle, and liver, all of which showed no reduction in SNAP23 levels or its major cognate Stx-4,
VAMPs, and Munc18c 2 weeks after AAV8-RIP1-Cre injection. Because RIP1-Cre expression could leak
into the brain (27), particularly the hypothalamus known to affect glucose homeostasis, we investigated and
found no SNAP23 in mouse brain (thus used as negative control in Figure 1F), including the hypothalamus
(Supplemental Figure 2A).
β Cell–specific deletion of SNAP23 in mice causes paradoxical improvement in glucose homeostasis resulting from
increased GSIS from pancreatic islets. AAV8-RIP1-Cre–induced deletion of β cell SNAP23 in SNAP23fl/fl
mice (henceforth called βSNAP23-KO) has the advantage of comparing glucose homeostasis from the
same mouse before and after viral transduction. It was initially assumed that SNAP23 plays a redundant
pro-exocytotic role to SNAP25 (16, 18), and hence its depletion in β cells would reduce GSIS in vivo.
Instead, βSNAP23-KO mice exhibited a paradoxical improvement in glucose homeostasis compared
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Figure 1. Generation of a mouse with β cell–specific deletion of SNAP23. (A) Whole islets from SNAP23fl/fl mice (24) show that SNAP23 is abundant in β
cells (confocal imaging), shown more clearly in the enlarged box 1. Scale bars: 100 μm. Quantitation can be found in Supplemental Figure 1A, bottom left.
(B) Single islet β cells from human (first row) and mouse (second row) show that SNAP23 is abundant in the insulin SGs. SNAP23 is partly colocalized with
Stx-1A (third row) and SNAP25 (fourth row) on the PM. Scale bars: 5 μm. Quantitation can be found in Supplemental Figure 1A, bottom middle. SNAP23
antibody controls (without primary antibody) are shown in Supplemental Figure 1A, top left, and A, top right. (C) SNAP23 is also present in glucagon-containing α cells located in the periphery of the mouse islet. Scale bars: 10 μm. Quantitation in Supplemental Figure 1A, bottom right. (D) AAV8-RIP1-Cre
drives Cre expression only in β cells (top) and not in α cells (bottom). Scale bars: 100 μm. Note the cytosolic insulin surrounding the nuclear Cre shown
clearly in the enlarged box 2. (E) Efficient knockdown of islet β cell SNAP23 expression is shown in (top) where Cre-positive cells are SNAP23-negative,
indicating SNAP23 deletion in those cells, and (bottom) the majority of insulin-positive cells are SNAP23-negative. The few SNAP23-positive cells are
likely α cells. Scale bars: 100 μm. (F) Western blots of islets from SNAP23fl/fl mice injected with the AAV8 (βSNAP23-KO) or not (Control) showed reduction of SNAP23 but not other exocytotic proteins. SNAP23 and VAMP8 are not abundant in mouse brain. Blots are representative from 3 independent
experiments; analysis of n = 3 in Supplemental Figure 1B. (G) βSNAP23-KO versus SNAP23fl/fl (Control) mice show SNAP23 to be reduced only in islets but
not in fat, muscle, or liver, wherein SNAP23 and cognate Munc18c and Stx-4 are putative exocytotic proteins. Shown are representative of 3 independent
experiments; analysis of n = 3 in Supplemental Figure 1C.
with SNAP23fl/fl mice (henceforth called Control) as shown by i.p. glucose tolerance tests (IPGTTs)
(Figure 2A, left), with corresponding large increases in insulin release (Figure 2A, right) encompassing
first-phase (<30 minutes) and second-phase (>30 minutes) GSIS. In contrast, additional control (SNAP23fl/fl) mice not treated with AAV8-RIP1-Cre virus monitored in parallel at 2 weeks with the virus-treated
group showed no changes in glucose homeostasis (including blood insulin levels; Supplemental Figure
2B). As shown in Figure 2B, i.p. insulin tolerance tests (IPITTs) revealed no effects on insulin sensitivity,
complementing our results in Figure 1G showing no reduction in SNAP23 levels in insulin-sensitive
tissues. Consistently, virus-transduced βSNAP23-KO did not affect body weight when compared with
pretreatment with virus (Figure 2C) and with untreated Control mice (Supplemental Figure 2C).
We then explored whether the increased GSIS in βSNAP23-KO mice is due to an increase in β cell
mass or increased secretory capacity of each β cell. Between the 2 groups of mice in Figure 2, D and E,
we saw no significant changes in islet number/pancreatic area (βSNAP23-KO: 2.67 ± 0.31 × 107 μm2;
Control: 2.98 ± 0.66 × 107 μm2), islet size (βSNAP23-KO: 37.76 ± 5.57 × 103 μm2; Control: 33.57 ± 4.79
× 103 μm2), and β cell area/pancreatic area (βSNAP23-KO: 0.0059 ± 0.0008; Control: 0.0066 ± 0.0009).
Therefore, the increased GSIS in vivo must be attributed to an increase in secretory capacity of the
SNAP23-depleted β cells.
What β cell exocytotic events account for the increased GSIS in βSNAP23-KO mice? We first isolated islets
from mice and conducted islet perifusion assays to assess physiologic biphasic GSIS (Figure 3A, left). Area
under the curve (AUC) analysis (Figure 3A, middle) showed that βSNAP23-KO islets exhibited increases of 144% in first-phase (βSNAP23-KO: 7.95 ± 0.74; Control: 3.26 ± 0.57) and 92% in second-phase
(βSNAP23-KO: 5.12 ± 0.64; Control: 2.66 ± 0.39) GSIS over Control islets. Total islet insulin content was
similar between the 2 (Figure 3A, right). We then employed 2 assays to assess the precise exocytotic events
upregulated by SNAP23 deletion to explain these increases in biphasic GSIS.
Patch-clamp membrane capacitance (Cm) measurements (28) with 10 serial depolarization pulses were
performed to determine single β cell exocytosis. In βSNAP23-KO β cells, there was a 216% increase in Cm
compared with control β cells (AUC analysis in Figure 3B, bottom right; βSNAP23-KO: 65.22 ± 9.31 fF/
pF; Control: 30.05 ± 9.37 fF/pF).
To visualize the spatiotemporal mobilization of populations of SGs and single-SG fusion dynamics, we employed time-lapse total internal reflection (TIRF) microscopy of SGs tagged with neuropeptide Y–EGFP delivered by adenovirus (Ad-NPY-EGFP), as previously described (6, 8). In Figure
3C, the larger increase in first-phase GSIS from βSNAP23-KO islets seems to be mainly attributable
to a 114% increase in exocytosis of predocked SGs (Figure 3C, right: βSNAP23-KO: 13.9 ± 3.23
events/100 μm2; Control: 6.49 ± 1.82), with no further increases in newcomer SG fusions. At basal
condition (Figure 3C, bottom right), there was, however, no significant difference in number of predocked SGs between the 2 groups (βSNAP23-KO: 11.7 ± 1.4 SGs/100 μm2; Control: 11.1 ± 0.8).
Our data therefore suggest that predocked SGs in βSNAP23-KO β cells were more fusion competent
than Control β cells. Predocked SGs, however, contribute very little to second-phase GSIS (6, 8). The
observed increase in second-phase GSIS from βSNAP23-KO islets is attributable to large increases in
no-dock (βSNAP23-KO: 4.82 ± 1.26 events/100 μm2; Control: 0.7 ± 0.39) and short-dock newcomer
SG fusions (βSNAP23-KO: 5.6 ± 2.2 events/100 μm2; Control: 1.0 ± 0.35). SNAP23 deletion therefore
increases the exocytotic efficiency of both predocked and newcomer SGs.
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Figure 2. β Cell–specific deletion of SNAP23 in mice improves glucose homeostasis resulting from increased GSIS from pancreatic islets. (A)
IPGTT performed in the same SNAP23fl/fl mice before AAV8-RIP1-Cre treatment and at 2 weeks after virus treatment showed improved glucose
homeostasis (left) resulting from increased insulin secretion (right). Right panels show the respective AUC analyses of n = 11. (B) IPITT performed
on the same mice before and after AAV8-RIP1-Cre treatment showed no significant changes in blood glucose levels, which indicates no effect on
insulin sensitivity. Right panel shows the AUC analysis of n = 11. (C) SNAP23-KO did not affect body weight. n = 11. (D) Insulin-immunostained
pancreatic sections (scale bars: 1000 μm), from which we conducted morphometric analysis (E), including β cell area per pancreatic area ratios,
islet number per total pancreatic area, and islet size (n = 12 for each group). *P < 0.05; ***P < 0.001. Statistical significance was assessed by 2-tailed
Student’s t test.
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Figure 3. β Cell exocytotic events that account for the increased GSIS in βSNAP23-KO mice. (A) Islet perifusion assays showing that βSNAP23-KO mouse
islets exhibited enhanced first- and second-phase GSIS compared with SNAP23fl/fl (Control) mouse islets; AUC analysis shown in middle. Left: Total islet
insulin content was not affected in the βSNAP23-KO mice islets (n = 5 for each group). (B) Patch-clamp Cm on dispersed single β cells of βSNAP23-KO
versus Control mice. Top shows representative recordings of exocytosis evoked by a train of ten 500-ms depolarizations from –70 mV to 0 mV. Bottom
left: Cumulative changes in cell capacitance normalized to basal cell membrane capacitance (fF/pF) in Control and βSNAP23-KO β cells, shown as (bottom
right) summary graph (Control: n = 15 cells, βSNAP23-KO: n = 13 cells). (C) TIRF microscopy imaging of exocytosis of predock and newcomer SGs. Left: Histograms of different fusion events in first phase (first 6 minutes after 16.7 mM glucose stimulation) and second phase (6–14 minutes) in Control (top) versus βSNAP23-KO β cells (bottom). Data obtained from 3 independent experiments (5–6 cells from each experiment; WT = 15 cells, βSNAP23-KO = 16 cells).
Right top: Summary of the 3 modes of fusion events in first (top) and second phases (bottom). This is the sum of the different types of SG fusion events
occurring during the first phase (all frames assessed in the first 6 minutes) and second phase (6–14 minutes) normalized to the PM area. Right bottom: We
also obtained TIRF microscopy images of docked insulin SGs before the stimulation (basal) above, and found no change in SG density (averaged number of
SGs normalized to cell PM area on each TIRF microscopy imaging frame before stimulation) between Control versus βSNAP23-KO β cells (summary graph
on right). Scale bars: 2 μm. *P < 0.05; **P < 0.01. Statistical significance was assessed by 2-tailed Student’s t test.
SNAP23 depletion increases GSIS in normal and T2D human islets. What are the implications of these
findings for human islet physiology and treatment of T2D? We depleted SNAP23 in human islets with
Ad-SNAP23-shRNA/mCherry, which reduced SNAP23-positive cells to just 9% of SNAP23-positive islet area (Figure 4A, left, Ad-scrambled shRNA/mCherry (henceforth called Control): 86 ± 6.7%;
Ad-SNAP23-shRNA/mCherry (henceforth called SNAP23-knockdown [SNAP23-KD]): 7.9 ± 3.9%). At
the islet protein level (Figure 4A, right and analysis of n = 3 in Supplemental Figure 4A), this was an 89% ±
5.5% reduction, and with no effects on expression of other exocytotic proteins. Remarkably, islet perifusion
(Figure 4B, top) and AUC analysis (Figure 4B, bottom) showed similarly large increases of 74.2% in firstphase (SNAP23-KD: 6.48 ± 0.33; Control: 3.72 ± 0.58) and 114% in second-phase GSIS (SNAP23-KD:
7.89 ± 1.1; Control: 3.68 ± 0.77). Cm measurements (Figure 4C) performed on SNAP23-KD (mCherry-positive) human β cells showed significant increases in every depolarizing pulse (Figure 4C, bottom left)
compared with Control cells (mCherry-positive), with a cumulative increase of 200% (SNAP23-KD: 58.42
± 12.02 fF/pF; Control: 29.16 ± 6.59 fF/pF; P < 0.01; Figure 4C, bottom right).
We first assessed by electrophysiology why SNAP23-KD increases exocytosis. Because SNAP25
binds and regulates β cell voltage-gated calcium channels (Cavs) (23, 29), SNAP23 might also affect
Cavs. Supplemental Figure 3A shows that SNAP23-KD in human β cells did not affect Cav current.
We then considered that SNAP23-KD might instead affect Ca2+ sensitivity of exocytosis, which was
assessed by Cm increases in response to flash photolysis of caged Ca2+. This would test the hypothesis that SNAP23 could confer a reduction in fusion competence and thus become less responsive
to Ca2+-mediated fusion, so SNAP23 depletion would relieve more fusion-competent SNAP25 molecules to more effectively mediate fusion. Flash photolysis of o-nitrophenyl–caged EGTA (NP-EGTA)
was used to induce a step-like, homogeneous increase in intracellular Ca2+ concentration [Ca2+]i from
200–300 nM to 5–10 μM. Averaged Cm traces were compared between control and SNAP23-KD cells
responding to similar step-like [Ca2+]i elevations (Supplemental Figure 3B, top). At low[Ca2+]i elevation (<10 μM), there is a fast component that releases earlier than the readily releasable pool, referred
to as the highly calcium-sensitive pool (HCSP) (30), which occurs within 200 ms after the flash spike
(Supplemental Figure 3B, middle). Curve-fitting analysis of the multiple components of Cm increases
demonstrates that SNAP23 deletion neither changed the kinetics of secretory response nor affected the
size and release rate of the HCSP. To confirm, whole-cell capacitance change recorded during direct
application of 200 nM or 1.5 μM free Ca2+ via patch pipette showed that SNAP23-KD also did not
affect exocytosis (Supplemental Figure 3B, bottom).
Human T2D islets were reported to exhibit reduced levels of some SNARE and SM proteins (31) (primarily Stx-1A, VAMP2, SNAP25, and Munc18a) to explain the insulin secretory deficiency in T2D, particularly the loss of first-phase release. Figure 5A (analysis of n = 3 in Supplemental Figure 4B) confirms
that although SNAP25, cognate Stx-1A, and Munc18a levels are severely reduced in T2D human islets (31),
SNAP23 levels are normal. Levels of Stx-3 (8), which mediate newcomer SGs in second-phase GSIS, and
Stx-4, which plays a role in both first- and second-phase GSIS (4, 9), were unaffected. SM protein Munc18b,
which binds Stx-3, also showed reduced levels, as we have recently reported (32). Because reduction in β cell
SNAP23 levels potentiated GSIS when levels of other exocytotic proteins are normal (Figure 4, A–C), we
asked the question whether SNAP23 depletion of T2D islets could rescue the deficient GSIS. We treated the
T2D human islets with Ad-SNAP23-shRNA/mCherry versus Ad-scrambled shRNA/mCherry (Control)
and conducted islet perifusion assays (Figure 5B). As is well known, GSIS from T2D human islets is much
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Figure 4. SNAP23 depletion increases GSIS in normal human islets. (A) Ad-SNAP23 shRNA/mCherry treatment (48 hours) of human islets (see Supplemental
Table 1 for details on human donors) effectively depleted SNAP23 (SNAP23-KD) as shown on confocal imaging (left) and islet protein levels (right). Scale bars:
100 μm. Left: SNAP23 is abundant in human islet β cells (top) and not affected by Ad-scrambled shRNA/mCherry (middle). SNAP23 was severely depleted by
Ad-SNAP23 shRNA/mCherry treatment (bottom), wherein the few SNAP23-positive cells are mCherry-negative, allowing calculation of the ratio of SNAP23-positive area per islet area (right graph, n = 19 islets from each group). Right: Western blots of islets show reduction in SNAP23 protein levels by Ad-SNAP23 shRNA/
mCherry but no effects on the other exocytotic proteins. Shown are representative of 3 experiments, with analysis of n = 3 in Supplemental Figure 4A. (B)
SNAP23-KD of human islets increased first- and second-phase GSIS compared with Ad-scrambled shRNA/mCherry–treated (Control) islets. AUC analysis in
bottom from n = 4 for each group, from 4 independent experiments. (C) These human islets were then dispersed into single cells for patch-clamp Cm measurements performed on mCherry-positive β cells, which showed that SNAP23-KD β cells exhibited increased exocytosis. Top: Representative recordings. Bottom left:
Cumulative changes in cell capacitance normalized to basal cell membrane capacitance (fF/pF) in Control versus SNAP23-KD (n = 15 cells) β cells, and shown as
(bottom right) summary graph (Control: n = 14 cells, SNAP23-KD: n = 15 cells). *P < 0.05; **P < 0.01; ***P < 0.001. Statistical significance was assessed by 2-tailed
Student’s t test.
reduced in both first- and second-phase GSIS (32). Remarkably, SNAP23 depletion of T2D human islets
increased first-phase GSIS by 89% (AUC analysis, Figure 5B, bottom; SNAP23-KD: 2.64 ± 0.57; Control:
1.4 ± 0.25) and second-phase GSIS by 103% (SNAP23-KD: 2.4 ± 0.28; Control: 1.18 ± 0.19), to levels
approaching the GSIS of normal human islets (Figure 4B).
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Figure 5. SNAP23 depletion increases GSIS in T2D human islets. (A) Western blots of T2D human islets (see Supplemental Table 1 for T2D patients’ donor
information) show reduction of Stx-1A and SNAP25 as previously reported (24), but with normal levels of SNAP23 and several other syntaxins. Shown are
representative of 3 experiments with analysis of n = 3 in Supplemental Figure 4B. (B) Ad-SNAP23 shRNA/mCherry treatment to deplete SNAP23 in T2D
human islets increased first- and second-phase GSIS compared with Ad-scrambled shRNA/mCherry (Control)-treated T2D islets. n = 4 for each group, from
4 independent experiments. *P < 0.05. Statistical significance was assessed by 2-tailed Student’s t test.
We performed the converse experiment in which SNAP23 was overexpressed in normal human islets
with Ad-SNAP23/mCherry and obtained a moderate increase of 269.3% ± 19.2% of normal islet levels,
which did not affect the expression of other exocytotic proteins (Figure 6A and analysis of n = 3 in Supplemental Figure 4C), but was able to cause a 49.2% reduction in first-phase GSIS (SNAP23 overexpression: 2.13 ± 0.93; Control: 4.19 ± 0.87; Figure 6B, top, and AUC analysis of n = 3 in Figure 6B, bottom)
and 48.9% reduction in second-phase GSIS (SNAP23 overexpression: 1.56 ± 0.87; Control: 3.05 ± 0.8).
Supplemental Figure 4D confirms that the overexpressed SNAP23-mCherry in human β cells surfaced
to its native location in the PM (phalloidin stain) where Stx-1A is abundant, and also colocalized with
Stx-3 that is abundant in insulin SGs, and not trapped in the Golgi complex. TIRF microscopy assay of
single-SG fusion dynamics (Figure 6C, top) showed that the decrease in first-phase GSIS was attributable
to a 50.1% reduction in exocytosis of predocked SGs (Figure 6C, bottom, SNAP23 overexpression: 2.83
± 0.82 events/100 μm2; Control: 5.67 ± 0.74) and newcomer SGs, including a 59.5% reduction in no-dock
SGs (SNAP23 overexpression: 3.44 ± 0.95; Control: 8.5 ± 2.49) and 90% reduction in short-dock SGs
(SNAP23 overexpression: 0.26 ± 0.05; Control: 2.58 ± 0.52). The decrease in second-phase GSIS caused
by SNAP23 overexpression was attributable to a 55.8% reduction in no-dock newcomer SGs (Figure 6C,
bottom; SNAP23 overexpression: 5.13 ± 0.49; Control: 11.6 ± 2.92) and 61.8% reduction in short-dock
newcomer SGs (SNAP23 overexpression: 2.54 ± 0.82; Control: 6.65 ± 1.86).
Pancreatic SNAP23 depletion in T2D GK rats rescues insulin secretory deficiency, restoring glucose homeostasis.
What is the implication of pancreatic islet SNAP23 depletion for restoring glucose homeostasis in T2D? GK
rats, a well-established nonobese T2D model closely mimicking human T2D (32), exhibit reduced biphasic
GSIS with normal peripheral tissue sensitivity to insulin, and which like human T2D (31), has been attributed
in part to severely reduced islet levels of SNARE proteins that mediate predocked SG exocytosis (33, 34).
We infused Ad-SNAP23-shRNA/mCherry versus Control Ad-scrambled shRNA/mCherry into T2D GK
rats via pancreatic duct (32, 35) (Figure 7). In a subset of GK rats, islets were isolated immediately after virus
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Figure 6. SNAP23 overexpression inhibits
GSIS in normal human islets and β cells. (A)
Ad-SNAP23/mCherry treatment (48 hours) of
human islets effectively overexpressed SNAP23
as shown on Western blots with no effects on
other exocytotic proteins. Shown are representative of 3 experiments with analysis of the n =
3 in Supplemental Figure 4C. (B) Islet perifusion
assays showing that SNAP23 overexpression
in human islets decreased first- and second-phase GSIS. AUC analysis of n = 3 from 3
independent experiments shown in bottom.
(C) TIRF microscopy imaging of exocytosis of
predock and newcomer SGs. Top: Histograms
of different fusion events in first-phase (first 6
minutes after 16.7 mM glucose stimulation) and
second-phase (6–14 minutes) in Control versus
SNAP23 overexpressing β cells. Data obtained
from 3 independent experiments (5–6 cells from
each experiment; control = 14 cells, SNAP23
overexpression = 15 cells). Bottom: Summary of
the 3 modes of fusion events in first and second
phases. *P < 0.05. Statistical significance was
assessed by 2-tailed Student’s t test.
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infusion and cultured for 3 days to allow shRNAs to work optimally. Ad-SNAP23-shRNA/mCherry–infused
GK rat islets showed a 68.4% reduction (Supplemental Figure 5A) in SNAP23 levels; otherwise untreated
GK rat islet SNAP23 levels were similar to Wistar rat islets. Islet levels of predocked SG exocytotic proteins
(Munc18a, Stx-1A, SNAP25, and VAMP2) in GK rats were confirmed to be severely reduced (Supplemental Figure 5A and refs. 33, 34), and newcomer SG exocytotic proteins Stx-3 (8), VAMP8 (6), and Munc18b
(32) were not affected by SNAP23 depletion, consistent with the results of the βSNAP23-KO mouse (Figure
1) and human islet SNAP23 depletion (Figure 4). Taken together, these results indicate that the resultant
increased GSIS in vivo in GK rats depleted of islet SNAP23 (Figure 7), as with βSNAP23-KO mice (Figures 2
and 3) and human islets SNAP23-KD (Figures 4 and 5), were due to β cell SNAP23 depletion per se and not
attributable to any effect on levels of other exocytotic proteins.
In fact, just 1 week after pancreatic ductal infusion with Ad-SNAP23-shRNA/mCherry into GK rats,
IPGTT already displayed improved glycemic control, with a corresponding increase in insulin secretion
(compared with Ad-mCherry in Figure 7A and compared with Ad-mCherry and before infusion in Supplemental Figure 5B). This improvement approached normoglycemia, peaking at 2 weeks after infusion
(Figure 7B), persisted at 4 weeks after infusion (Figure 7C), but becoming less so at 8 weeks after infusion
(Figure 7D) due to reduced adenovirus expression at 8 weeks (32, 35). Control virus–treated (Ad-Cherry–
treated) GK rats were similarly hyperglycemic at 1 and 2 weeks after infusion (Figure 7, A and B) as the preop state (Supplemental Figure 6, A and B), and displayed worsening hyperglycemia at 4 and 8 weeks after
infusion (Figure 7, C and D, and Supplemental Figure 6, C and D), with corresponding progressive reduction in blood insulin levels. Insulin tolerance tests (ITTs) (Supplemental Figure 5C) showed slight worsening of insulin resistance of GK rats at 10 weeks after infusion compared with the pre-op normal insulin
tolerance of GK rats, but there was no difference between Ad-SNAP23-shRNA/mCherry and control virus
treatments, suggesting that the increased insulin resistance was likely attributable to postoperative stress.
We compared the effects of Ad-SNAP23shRNA/mCherry pancreatic ductal infusion treatment of GK
rats to a standard treatment for T2D, which is oral gavage with a long-acting sulfonylurea glybenclamide at
the optimal dose of 2 mg/kg (ref. 36 and Figure 7). Whereas glybenclamide treatment was superior to vehicle treatment (Supplemental Figure 6), there was progressive reduction in glybenclamide-stimulated insulin release with corresponding worsening of glycemic control. In contrast, Ad-SNAP23-shRNA/mCherry
treatment was initially equal to glybenclamide treatment at 1 week after infusion (Figure 7A), and then
became superior at 2 weeks after infusion (at peak response), which persisted at 4 and 8 weeks after infusion
in stimulating insulin release, with corresponding improved glycemic control (Figure 7, B–D). At 8 weeks
after infusion (Figure 7D), showing overall reduced insulin secretion from all groups, the Ad-SNAP23-shRNA/mCherry group still showed GSIS that was 108% greater than the control virus group compared with
a 42% increase in the glybenclamide group (AUC: Ad-SNAP23-shRNA/mCherry: 85.2 9.1; Ad-mCherry:
40.9 ± 5.5; glybenclamide: 60.1 ± 4.9), and with corresponding superior glycemic control.
Some of these rats were sacrificed after IPGTT, and the pancreases then resected for histological analysis
(Supplemental Figure 5D). β Cell mass and distribution in GK rat islets with or without SNAP23 depletion
were very similar compared with those of pancreases before infusion. This indicates that the large increase
in insulin output caused by SNAP23 depletion versus the smaller output from glybenclamide treatment was
due to increased insulin secretion per β cell and not by any changes in β cell mass or islet morphology.
SNAP23 depletion enables endogenous SNAP25 to promote formation of more fusion-competent fusion complexes.
We explored for a possible mechanism to explain the increase in insulin SG exocytosis caused by SNAP23
depletion by performing protein-binding studies to assess SM/SNARE complexes formed with SNAP23 and
SNAP25. INS-832/13 cells were used instead of islets, as this provides an abundance of proteins required
for this assay. In Figure 8A, anti-SNAP23 antibody pulled down from INS-832/13 cells the same 3 exocytotic SM/SNARE complexes that interact with SNAP25 (4, 6, 17), including Munc18a/Stx-1A/VAMP2,
Munc18b/Stx-3/VAMP8, and Munc18c/Stx-4/VAMP2, and several proteins within these complexes
increased with 16.7 mM glucose plus 10 nM glucagon-like peptide 1 (GLP-1) stimulation (analysis in Supplemental Figure 7A). When SNAP23 levels were reduced by 83% with Ad-SNAP23-shRNA/mCherry, the
resulting SM/SNARE complexes pulled down with anti-SNAP25 antibody were increased (Figure 8B), particularly including those SM/SNARE complexes Munc18a/Stx-1A/VAMP2 and Munc18b/Stx-3/VAMP8
that mediate exocytosis of predocked SGs and newcomer SGs (2, 6, 17), respectively (analysis in Supplemental
Figure 7B). Munc18c and Stx-4 levels were not increased (data not shown). These results suggest that SNAP23
may be competing with SNAP25 (and vice versa) to form complexes with these cognate SNARE proteins.
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Figure 7. Pancreatic SNAP23 depletion in type-2 diabetic GK rats rescues insulin secretory deficiency and restores glucose homeostasis. Ad-SNAP23 shRNA/
mCherry (6.6 × 109 PFU) versus Ad-mCherry (same dose) was infused via pancreatic duct into GK rats. IPGTTs (blood glucose and insulin levels obtained) were performed after infusion at 1 (A), 2 (B), 4 (C), and 8 weeks (D). Glybenclamide treatment of age- and sex-matched GK rats as conventional treatment for T2D was also
performed; IPGTT was then performed at the same time points. Graphs on the right show AUCs encompassing 180 minutes of the IPGTTs. Glybenclamide group:
n = 6 and Ad-SNAP23 shRNA/mCherry group: n = 6 for 1, 2, and 4 weeks after infusion, and n = 5 for 8 weeks after infusion. Ad-mCherry control groups: n = 6 for 1
and 2 weeks after infusion, and n = 5 for 4 and 8 weeks after infusion. We also performed vehicle control in GK rats compared with glybenclamide treatment, and
conducted IPGTTs as shown in Supplemental Figure 6. *P < 0.05; **P < 0.01; ***P < 0.001. Statistical significance was assessed by repeated-measures ANOVA.
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Figure 8. SNAP23 acts as inhibitory SNARE that reduces SNAP25 binding to Stx-1A and Stx-3 from forming SNARE complexes, whereby these SNAP25
but not SNAP23 SNARE complexes could form more fusion-competent machineries with Cav, priming proteins and Ca2+ sensors. Coimmunoprecipitation (Co-IP) studies were performed on INS-832/13 cells before stimulation (basal) and after maximal stimulation with 16.7 mM glucose plus 10 nM
GLP-1. (A) SNAP23 forms similar SM/SNARE complexes as those previously reported with SNAP25. Co-IP was performed with SNAP23 antibody. Shown
are representative of 3 experiments with analysis of the 3 experiments shown in Supplemental Figure 7A. (B) SNAP23 depletion in INS-832/13 cells
enables endogenous SNAP25 to promote formation of more SM/SNARE complexes to mediate the increased exocytosis of predocked SGs (Munc18a,
Stx-1A, VAMP2) and newcomer SGs (Munc18b, Stx-3, VAMP8) that underlie the increased first- and second-phase GSIS shown in Supplemental Figures
2–4. INS-832/13 cells were first treated with Ad-SNAP23 shRNA/mCherry to deplete SNAP23 versus Ad-scrambled shRNA/mCherry (Control). Co-IP was
performed with SNAP25 antibody. Shown are representative of 3 experiments, with analysis of the 3 experiments shown in Supplemental Figure 7B. (C)
SNAP23 blocks SNAP25 (and vice versa) binding to Stx-1A and Stx-3. HEK293 cells transfected with Stx-1A, Stx-3, SNAP23, or SNAP25 were subjected to
pulldown with GST-SNAP23 (in the presence or absence of expressed SNAP25) or GST-SNAP25 (in the presence or absence of expressed SNAP23) to assess
how much Stx-1A (top) or Stx-3 (bottom) could be precipitated. GST was used as control. Shown are representative of 4 experiments, with analysis of the
4 experiments shown in Supplemental Figure 7C. SNAP23 SNARE complexes (D) are less able than SNAP25 complexes (E) to pull down Cavs (Cav1.2, -1.3,
and -2.3) synaptotagmins (Syt-1 and -7) and priming proteins (RIM2, Munc13-1). Note that 16.7 mM glucose plus GLP-1 increased the binding of some of
these proteins in SNAP25 but not SNAP23 complexes. This is a representative of 3 separate experiments with analysis shown in Supplemental Figure 8.
(F) Control (Ad-scrambled shRNA/mCherry) or SNAP23-knockdown (Ad-SNAP23 shRNA/mCherry) human β cells were stimulated with 16.7 mM glucose
plus 10 nM GLP-1, immunolabeled with SNAP25 and Stx-1A or Stx-3 antibodies, and then assessed by structured illumination microscopy imaging. SNAP23
depletion increased SNAP25 colocalization with Stx-1A (top; Pearson’s correlation coefficient [PCC], Control: –0.42 ± 0.09; KD: 0.36 ± 0.04, n = 11 from 2
independent experiments) and Stx-3 (bottom; PCC, Control: –0.74 ± 0.06: KD: 0.14 ± 0.02, n = 12 from 2 independent experiments). Scale bars: 2 μm. ***P
< 0.001. Statistical significance was assessed by 2-tailed Student’s t test.
We assessed this by expressing Stx-1A and/or Stx-3 in HEK293 cells and measured how much of these syntaxins were pulled down by GST-SNAP23 (±SNAP25) or GST-SNAP25 (±SNAP23) (Figure 8C). SNAP23
reduced GST-SNAP25 binding to Stx-1A by 39% and to Stx-3 by 52% (analysis in Supplemental Figure 7C).
However, SNAP25 also reduced to a similar extent (analysis in Supplemental Figure 7C) GST-SNAP23 binding to Stx-1A (by 48%) and Stx-3 (by 65%). To translate these binding studies performed in cell lines into bona
fide spatiotemporal functional interaction studies in human β cells, Figure 8F shows that after knockdown
of SNAP23 expression with Ad-SNAP23-shRNA/mCherry (48 hours treatment, cytosolic mCherry signal
was used to identify the infected β cells), high-resolution single-molecule imaging by structured illumination
microscopy showed that stimulation (16.7 mM glucose + 10 nM GLP-1 for 10 minutes) increased the colocalization of endogenous SNAP25 (labeled with Alexa Fluor 647) on the PM with the endogenous Stx-1A
(Pearson’s correlation coefficient increased from –0.42 ± 0.09 to 0.36 ± 0.04) and Stx-3 (Pearson’s correlation
coefficient increased from –0.74 ± 0.06 to 0.14 ± 0.02) compared with control.
These results support our thinking that in the context of the β cell, SNAP23 is a weaker (partial agonist)
fusion SNARE or possibly an inhibitory SNARE (22, 37), the latter becoming manifest when SNAP25 levels
are low, as in T2D islets (31–34). Taken together with the βSNAP23-KO TIRF microscopy study (Figure 3C),
our results suggest that SNAP23-driven SNARE complexes may be less fusion competent than SNAP25-driven
complexes. We then assessed why this might be the case. The fusion machinery involves not only SNARE
complexes but also very complex interactions with Cavs to form excitosomes (23) that position SGs to fuse at
PM sites of maximal Ca2+ influx, and additional priming proteins (RIM2, refs. 38, 39; Munc13-1, refs. 40, 41)
and Ca2+ sensors (synaptotagmins 1 and 7 (Syt-1 and -7) (42, 43) known to optimize fusion competence (1, 2).
We performed a more exhaustive analysis of SNAP23 (Figure 8D and Supplemental Figure 8A) and SNAP25
coprecipitated proteins (Figure 8E and Supplemental Figure 8B). Both SNAP25 and SNAP23 coprecipitated
Cav1.2 and Cav2.3. SNAP25, not SNAP23, precipitated RIM2 and Munc13-1. SNAP23 precipitated small
amounts of Syt-1, whereas SNAP25 pulled down abundant Syt-1 and Syt-7. Importantly, physiologic glucose
plus GLP-1 stimulation increased SNAP25 (but not SNAP23) pulldown of Cav1.2, Cav 2.3, Syt-1, and Syt-7.
SNAP23 depletion promotes more SNAP25 to move more rapidly and stay longer on Cavs to increase exocytotic
efficiency. The biochemical data in Figure 8 are insufficient to prove but nevertheless suggest why SNAP25
machinery might be more fusion competent than the SNAP23 machinery. We thus proceeded to functionally
demonstrate the nature of this improved fusion competence caused by SNAP23-KO (Figure 9A) compared
with SNAP25 overexpression (Figure 9B). We employed high-resolution dual-color single-molecule imaging
(see Methods) to visualize the spatiotemporal interactions between individual SNAP25 molecules and Cavs
(the prototypical L-type Cav1.2; refs. 27, 41) at the PM surface of live INS-832/13 cells. SNAP25 tagged with
mScarlet was transiently expressed at low densities in Cav1.2-mNeonGreen–knockin INS-832/13 cells (see
Supplemental Figure 9B). SNAP25 molecules showed 2 distinct behaviors, either high motility or relative
immobility, the latter resulting from taking residence on a Cav1.2 (Figure 9, A and B). We estimated the
frequency and duration of SNAP25-Cav1.2 interactions, whereby within 5 seconds after 8 mM glucose stimulation (Figure 9C), the average cumulative interaction time between SNAP25 and Cav1.2 in SNAP23-KO
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Figure 9. Live-cell single-molecule localization and tracking of SNAP25-mScarlet binding to endogenous Cav1.2. (A) SNAP23-knockout (SNAP23-KO)
INS-832/13 cells. (B) SNAP23-overexpressing (OE) INS-832/13 cells. Representative live-cell single-molecule image of SNAP25-mScarlet and Cav-mNeonGreen in SNAP23-KO (A, top left) and SNAP23-OE (B, top left) INS-832/13 cells; these images are representative of 4 independent experiments. (A, top
left) Endogenous Cav1.2 in SNAP23-KO cells (mTagBFP2) was labeled with mNeonGreen by an MMEJ-mediated knockin method as previously described
(54) and shown in detail in Supplemental Figure 9 and described briefly in the Methods. Right: The trajectories of SNAP25-mScarlet were overlaid onto the
locations of the endogenous Cav1.2 clusters (indicated in red circles). (A, top right) Representative intensity traces of single SNAP25-mScarlet molecules
binding to Cav1.2 clusters (from top to bottom, white circles 1–3 indicated in A, top left). (A, bottom left) Examples of sequential images of the mobility of
single SNAP25-mScarlet coming from the cytoplasm (top: corresponds to white circle indicated as event 4 in A, top left) or along the PM (bottom: corresponds to white circle indicated as event 1 in A, top left) traveling to bind a Cav1.2 cluster; the latter trajectory shown in (A, bottom right). Blue rectangle
indicates the start, red circle indicates the location of Cav1.2. (B, top left) Cav1.2-knockin INS-832/13 cells were transfected with SNAP23-SNAPf for 24
hours and then incubated with far-red fluorescent substrate, 647-SiR, for imaging. SNAP25-mScarlet was transfected again for 3 to 6 hours before imaging. Trajectories of SNAP25-mScarlet were overlaid onto the locations of endogenous Cav (indicated as red circles). (B, top right) Representative intensity
traces (from top to bottom, white circles 1–3 indicated in B, top left). (B, bottom left) Two examples of sequential images showing the mobility of single
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SNAP25-mScarlet molecules coming from the cytoplasm (top: corresponds to white circle indicated as event 2 in B, top left) or along the PM (bottom:
corresponds to white circle indicated as event 4 in B, top left) traveling to bind Cav1.2 clusters; the latter trajectory shown in B, bottom right. (C) Comparison between SNAP23-KO versus WT versus SNAP23-OE. Left: Cumulative dwell time of SNAP25 binding to Cav1.2 per Cav1.2 clusters. Middle: Cumulative
frequency of SNAP25 binding to Cav1.2 per Cav1.2 clusters. Right: Travel time histogram of single events of SNAP25 binding to Cav1.2 (n = 10 each from 4
independent experiments). *P < 0.05. Statistical significance was assessed by repeated-measures ANOVA.
was 41% longer than SNAP23-overexpressing cells (left, KO: 0.31 ± 0.03 seconds, overexpressing: 0.22 ±
0.02 seconds) and SNAP25 binds to Cav1.2 with an 88% increase in the frequency (middle, KO: 0.79 ± 0.09,
overexpressing: 0.42 ± 0.08), indicating a higher binding affinity of SNAP25 for Cav1.2. in SNAP23-KO than
SNAP23-overexpressing cells. Correspondingly, SNAP25 travel time to Cav1.2 was much shorter in SNAP23KO cells (Figure 9C, right; also compare the trajectories in Figure 9A, bottom right, and Figure 9B, bottom
right). Control cells using a blank vector to infect WT INS832/13 cells showed similar (very slightly but
insignificantly better) frequency, travel time, and duration of SNAP25-Cav1.2 interactions as SNAP23-overexpressing cells (Figure 9C).
This increased SNAP25-Cav1.2 binding affinity caused by SNAP23 depletion would be expected to
improve fusion competence, which was tested in Figure 10, assessing the spatial relationship between insulin
SG fusions (NPY-tdmOrange2; ref. 44) and Cav1.2. Compared with Control, SNAP23-KO results in increased
average SG exocytosis on Cav1.2 over time (Figure 10E), with estimated probability of Cav1.2-localized SG
fusion per channel cluster in SNAP23-KO cells to be 41% higher (Figure 10E, SNAP23-KO: 0.24 ± 0.02/min,
Control: 0.17 ± 0.02/min). These quantitative results indicate that SNAP23 depletion increases the amount,
speed, and duration of SNAP25 residence on Cav1.2, which enhanced SG exocytotic efficiency at the Cavs.
Discussion
SNAP23 forms the same SNARE complexes as SNAP25, and is thus expected to mediate exocytosis in
β cells as in slower secretory cells like acini (14) and adipocytes (13) that contain only SNAP23. In vitro
fusion assays have confirmed that SNAP23 can mediate membrane fusion (19, 20). Our work showing
that β cell SNAP23 depletion paradoxically increased biphasic GSIS by increasing fusion of predocked
and newcomer SGs in more secretory-efficient pancreatic β cells indicates that the abundant endogenous
SNAP25 could be relieved to exert its actions in mediating more efficient SG fusion at the Cav than that
mediated by SNAP23 (Figure 10). The reason for this is that SNAP23-KO enables not only more SNAP25
molecules to occupy Cav1.2 clusters (Figure 9) as well as bind Stx-1A and Stx-3 (Figure 8F) to form excitosomes, but SNAP25 moved faster and stayed on longer on Cav1.2 (Figure 9). Peculiarly, this enhanced
residency of SNAP25 on Cav did not alter channel activity or Ca2+ sensitivity of fusion.
In the most efficient secretors, neurons, SNAP25 is the only putative cognate SNARE partner of Stx1A and VAMP2 in mediating synaptic vesicle release from presynaptic nerve terminals (1, 21), although
SNAP23 has been found in dendritic spines (45). Nonetheless, SNAP23, exogenously expressed in
SNAP25-deleted chromaffin cells, could not support primed SGs that were well supported by SNAP25 (21).
SNAP23 exogenously expressed in SNAP25-deleted neurons (neurons do not contain SNAP23) could only
support asynchronous release (46), and was shown to require the Ca2+ sensor Syt-7 (47). Interestingly, Syt-7
is the major Ca2+ sensor for insulin SG exocytosis in β cells (42, 43). In contrast, Syt-1, the major Ca2+ sensor
in neurons for synchronous release (48), could effect efficient fusion with the Stx-1A/SNAP25 complex in
in vitro fusion assays, but when replaced with SNAP23, fusion became less efficient (20). In fact, Syt-1 had
little effect on VAMP2-to-SNAP23 vesicle fusion (20). Taken together, these results show that SNAP23 is a
less efficient fusion protein than SNAP25, which serves its purpose in slower secretory cells like acini (14),
muscles, and adipocytes (4, 13). However, when both SNAP23 and SNAP25 are abundantly present, our
data in Figure 3, B and C, suggest that the less fusion-competent SNAP23 competes with fusion-competent
SNAP25 to reduce SNAP25 efficiency in forming exocytotic complexes.
Our results differ from a just-published study by Kunii et al. using a genetically deleted β cell SNAP23KO mouse (49), in contrast with our AAV8-RIP1-Cre virus 2-week-induced knockdown of SNAP23 in
SNAP23fl/fl mice, which showed unequivocal knockdown of only β cell SNAP23. The study by Kunii et al.
showed that SNAP23 deletion only increased predocked SG fusion along with a corresponding increase in
predocked SG SNARE complexes underlying an increased first-phase GSIS (49). Our study showed that
SNAP23 deletion also increased newcomer SG fusions by increasing newcomer SG SNARE complexes,
which also increased second-phase GSIS. Hence, SNAP23 deletion in β cells resulted in potentiation of
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Figure 10. Individual insulin SG exocytosis at Cav1.2 clusters. (A) Control INS-832/13 cells. (C) SNAP23-knockout cells.
Data are representative of 3 independent experiments. Scale bars: 2 μm. (B) Representative montage of SG exocytosis
at Cav1.2 clusters. (D) Cumulative exocytosis events per Cav1.2 cluster along the different time points during stimulation.
(E) Average exocytosis events per Cav1.2 cluster per minute (12 cells each from 3 independent experiments). *P < 0.05.
Statistical significance was assessed by 2-tailed Student’s t test.
biphasic GSIS not only in normal mouse (Figure 2; also shown in the Kunii et al. study) and human islets
(Figure 4), but our work further showed near-normalization of the deficient GSIS in T2D human islets
(Figure 5) and GK rat islets in vivo with a single treatment of a SNAP23 shRNA–containing virus, achieving a sustained effect of a long duration of 4 months that exceeded conventional glybenclamide treatment
(Figure 7). In the latter, the improved glucose homeostasis was sustained for 3 hours after glucose load,
encompassing the effects of improved biphasic GSIS. This is different from the merely first-phase (but
not second-phase) GSIS improvement in the Kunii et al. study. The latter indicates a genuine therapeutic
potential for T2D compared with just amplifying first-phase GSIS in the Kunii et al. study, and implies that
even the very low abundance of residual SNAP25 (Figure 5A), when not competed with by SNAP23, could
bind low levels of the cognate SNAREs Stx-1A and VAMP2 in T2D islets (refs. 31, 33, 34; Figure 5A) to
still mount sufficient exocytotic machinery to effect near-normal GSIS. Another possibility to explain the
improved GSIS in SNAP23-depleted T2D islets is that normal islet levels of Stx-3 (8) and Stx-4 (50) (Figure
5A) could compensate for Stx-1A deficiency.
Our work thus suggests that SNAP23 acts as a weak partial fusion agonist in some secretory cells, but in
the context of β cells, SNAP23 effectively acts as an antagonist to SNAP25-mediated fusion. Our single-molecule imaging study showed that SNAP23 depletion increased SNAP25 localization to Stx-1A and Stx-3
(Figure 8F) to form SNARE complexes (Figure 8, A–C). We further provide a biochemical basis for the
superior fusion competence of SNAP25 compared with SNAP23. Figure 8, D and E, shows that SNAP25
and/or its SNARE complex have greater capacity to bind Cavs to form excitosomes to position SGs to Cavs
where fusion occurs (23), which we confirmed in Figure 10. Importantly, SNAP25 or its SNARE complex
has even greater capacity to bind major priming proteins (RIM2, Munc13-1) and Ca2+ sensors (Syt-1, Syt-7),
for which SNAP23 or its SNARE complex has low to no affinity. This is likely a basis of the superior fusion
competence of SNAP25 over SNAP23. While there are limitations to interpreting coimmunoprecipitation
studies to indicate mechanistic interactions, which require complex direct molecular interactions studies,
we have shown some single-molecule imaging data for SNAP25-Stx-1A/Stx-3 (Figure 8F) and SNAP25Cav interactions (Figure 9); these biochemical studies are likely physiological because glucose stimulation
increased binding to some of these proteins including Syt-7 and Cavs, along with cognate Munc18 and
SNARE proteins. These precise mechanistic insights were not elucidated in the Kunii et al. study (49).
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Teleologically, SNAP23 can act as a cell context–specific inhibitory SNARE (21) when it coexists
with SNAP25 to better fine-tune membrane fusion. In β cells, SNAP23 works on slow insulin release and
SNAP25 acts on fast insulin release. This slow insulin release may regulate the fast insulin release mechanism in a cell-autonomous manner. Removal of the slow release mechanism by SNAP23-KD removes this
autoregulatory mechanism. Peculiarly, in T2D human islets, SNAP23 levels are not reduced, whereas there
is a severe reduction in SNAP25 levels (discussed above). This suggests that SNAP23 would exert a stronger
antagonism against the residual SNAP25 in T2D β cells than when SNAP25 levels are abundant in normal
islets. Alternatively, insulin SG exocytosis in T2D human β cells employing SNAP23 would be less efficient because of slower fusion kinetics mediated by SNAP23, which might partly explain why there is still
substantial, albeit reduced, possible SNAP23-mediated second-phase GSIS from T2D islets. This would
suggest a shift from regulated fast SNAP25-mediated insulin release in normal islets to slow SNAP23-mediated insulin release in T2D islets. It is possible that the less regulated SNAP23-mediated release contributes
to the higher basal insulin release observed in T2D.
This concept of an inhibitory SNARE (22) has never been demonstrated to our knowledge in vivo in a
manner affecting whole-body homeostasis until our recent and current work. While rapid, short-burst patterns of exocytosis are characteristic of neurons, a slower and more prolonged secretory pattern of minutes
to several hours is the norm for β cells and other endocrine cells. These dual SNAP25 isoforms provide a
push-pull mechanism (with the push mediated by SNAP25 and pull by SNAP23) designed to deliberately
reduce the efficiency of insulin exocytosis for the purpose of conferring a sustained, metered release. Because
SNAP23 forms the same SNARE complexes as SNAP25, the reduced pull from SNAP23 depletion enabled
SNAP25 to form more fusion-competent SNARE complexes (with priming proteins and Ca2+ sensors), thus
providing more push to mediate exocytosis of predocked SGs (with Stx-1A and VAMP2) and newcomer SGs
(with Stx-3 and VAMP8) (2, 6, 8) that underlie first- and second-phase GSIS, respectively. This would explain
why SNAP23 depletion in normal and T2D human islets increased biphasic GSIS. Stx-2 likely also acts as
an inhibitory SNARE to compete with Stx-1A and Stx-3 (37), which would explain why β cell Stx-2 deletion
promoted exocytosis of Stx-1A–mediated predocked and Stx-3–mediated newcomer SGs. Teleologically, the
presence of inhibitory SNAREs serves to modulate different exocytotic efficiency between secretory cell types,
such as fast-secreting neurons (predominantly SNAP25) versus moderately-fast-secreting endocrine cells (both
SNAP25 and SNAP23) versus slow-secreting acinar cells and adipocytes (predominantly SNAP23).
Our results present intriguing possibilities for potential therapies for diabetes such as reducing pancreatic islet SNAP23 levels with an shRNA-containing virus in T2D patients in vivo by endoscopic perfusion
of the virus, which should mimic this study with GK rat pancreatic ductal perfusion (Figure 7), wherein a
single shot lasted a remarkable 4 months. Alternatively, isolated human islets could be treated with the shRNA-containing virus in vitro for use in transplantation in type-1 diabetes patients. The latter could allow a
smaller number of surviving enhanced-secreting β cells with enough insulin secretory capacity to achieve
glucose homeostasis, as suggested in Figure 4. Adenovirus, the effects of which begin to wane after just 2
months (this study), is of course not the ideal gene transfer strategy; a longer-acting, single-shot, safer gene
transfer approach recently shown with an AAV carrying a β cell promoter (26) will be required to proceed
to human trials (51). This also raises the possibility of employing strategies for specific manipulation of
SNAP23 or SNAP25 levels in other secretory cells to treat the corresponding diseases of oversecretion
(then overexpress SNAP23 to slow down secretion) or undersecretion (then overexpress SNAP25 to accelerate secretion). Alternatively, a small compound like MF286, as reported in the Kunii et al. study, which
binds specifically to SNAP23 to block SNARE complex assembly, was shown to promote GSIS in mice
(49). Caution should, however, be taken in these approaches, as distinct functions of SNAP23 and SNAP25
are likely dependent on other cognate exocytotic proteins shown in Figure 8, D and E, and others yet undefined, which may further alter SNAP23 or SNAP25 actions in a cell context–specific manner.
Methods
Antibodies and reagents
SNAP23 (catalog 111203), Stx-2 (catalog 110123), Stx-3 (catalog 110033), Stx-4 (catalog 110043), and tubulin
(catalog 302008) antibodies were procured from Synaptic Systems; Stx-1A (catalog SAB5600255), insulin
(catalog SAB4200691), and glucagon (catalog SAB4200685) antibodies were from Sigma-Aldrich; SNAP25
antibody was from Sternberg Monoclonal (Covance); Munc18a antibody (catalog 610336) was from BD
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Transduction Laboratories; and Cre antibody (catalog PRB-106P) was from Covance. As gifts, Munc18b was
from V. Olkkonen (Minerva Foundation Institute, Biomedicum, Helsinki, Finland), Munc18c antibody was
from D. Thurmond (City of Hope, Duarte, California, USA), VAMP2 antibody was from A. Lowe (Stanford
University, Stanford, California, USA), and VAMP8 antibody from W. Hong (Institute of Molecular and Cell
Biology, Singapore). Alexa Fluor 488 (catalog A11008), Alexa Fluor 647 (catalog A32728), Alexa Fluor 647
phalloidin (catalog A22287), GM 130 antibody (Golgi marker) (catalog PA1-077), FITC (catalog 711900), and
Texas Red (catalog A6399) were from Thermo Fisher Scientific. All other chemicals, unless specified, were
obtained from Sigma-Aldrich. Ad-SNAP23-human shRNA-mCherry (shRNA sequence: CCGGGAGTCTGGCAAGGCTTATAAGCTCGAGCTT ATAAGCCTTGCCAGACTCTTTTTG and Ad-SNAP23-mouse/
rat shRNA-mCherry (shRNA sequence: CCGGGAACAACTAAATCGCATAGAACTCGAGTTCTATGCGATTTAGTTGTTCTTTTTG were purchased from Vector Biolabs.
Generation of SNAP23fl/fl mice and β cell–specific SNAP23-deleted mouse SNAP23fl/fl mice were generated by Ozgene, as we have recently reported (24). Briefly, a targeting construct was generated that contained 5.9 and 4.6 kb upstream of exon 2 and downstream of exon 4, respectively. LoxP sites were inserted,
flanking exons 3 and 4, and the neo selection cassette was flanked by FRT sites. Following homologous
recombination, ES implantation and generation of SNAP23fl/fl mice in the C57BL/6J background, the
neo cassette was removed by crossing with FLP-recombinase mice. This mouse strain has been continually maintained in the C57BL/6J background for over 10 generations. Instead of crossing the SNAP23fl/fl
mouse with a RIP1-Cre mouse to embryologically delete SNAP23 in β cells, we employed the AAV8-RIPCre to delete β cell SNAP23 in the adult mouse. The viral construct contains 410 bp of rat INS1 promoter,
including the first exon, first intron, and UTR of exon 2, followed by Cre recombinase ORF and an SV40
polyA signal. Recombinant dsAAV8 production to a titer of 1 × 1013 plaque-forming units (PFU)/mL was
outsourced to The Center of Cellular and Molecular Therapeutics at the Children’s Hospital of Philadelphia Research Vector Core (Philadelphia, Pennsylvania, USA). AAV8-RIP1-Cre at a dose of 1 × 1012 PFU
was injected into the peritoneum (i.p.) of 8- to 10-week-old SNAP23fl/fl male mice (C57BL/6J background).
Two weeks after the i.p. injection, SNAP23 levels in the islet β cells were greatly reduced (see Results) and
remained so for several months, encompassing the in vivo experimental period performed on these mice
and in vitro studies on the islets isolated from these mice performed as we have described (6, 17).
Human islet and β cell SNAP23 knockdown and overexpression
Human islets were from institutional review board–approved healthy (10 donors) and diagnosed T2D
donors (5 donors), as shown in Supplemental Table 1, with either written informed consent from the donor
or the next of kin. Control (scrambled shRNA/mCherry), Ad-SNAP23 shRNA/mCherry (human and
mouse), and Ad-SNAP23/mCherry and Control (Ad-mCherry) were from Vector Biolabs. Islets or dispersed β cells were infected with Ad-SNAP23 shRNA/mCherry or Ad-SNAP23/mCherry for 48 hours to
knock down or increase SNAP23 expression, respectively, with parallel infections with their respective control viruses. Entry of viral particles after transduction was determined by mCherry fluorescence expression.
Confocal immunofluorescence microscopy
This was performed as previously described (6, 8, 17) using primary antibodies at 1:200 dilution and corresponding secondary antibodies. Immunostained cells mounted on glass coverslips were examined using
a Leica DMIRE2 inverted fluorescence microscope containing a Hamamatsu spinning disk confocal scan
head and back-thinned EM-CCD camera. Data acquisition and analysis were performed using Volocity
software (PerkinElmer). A 20× objective lens was used for whole-islet imaging and 63× objective lens for
single β cell imaging. All images were deconvoluted by Volocity to remove background noise.
Immunoprecipitation, in vitro binding, and Western blotting
Immunoprecipitation. Similarly to previously described methods (6, 32), INS-832/13 cells (gift from
C. Newgard, Duke University, Durham, North Carolina, USA) infected with Ad-SNAP23 shRNA/
mCherry or control virus, after 48 hours were then stimulated (or not) with 16.8 mM glucose plus 10
nM GLP-1. Cell lysates, solubilized in immunoprecipitation (IP) buffer, were centrifuged (55,000 g,
40 minutes) to remove insoluble materials. The detergent pellet extract (600 μg protein) was then precleared (35 μL protein A agarose [50% slurry], 1.5 hours) and incubated with protein A agarose–crosslinked anti-SNAP25 antibody (2 μg, 4°C, 1.5 hours).
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In vitro binding. In vitro binding was performed as previously described (52). GST (as control), GSTSNAP23, or GST-SNAP25 (250 pmol protein) bound to glutathione-agarose beads was incubated with
lysate extract (400 μg protein) of HEK293 cells transfected with Stx-1A, Stx-3, SNAP23, or SNAP25 in
lysis buffer for 2 hours at 4°C. The beads were then washed 3 times.
Western blotting. The precipitated proteins from IP and binding from the above were separated in 12%–
15% SDS-PAGE and identified with the indicated primary antibodies. Separately, whole pancreas, pancreatic islets, brain, fat, muscle, and liver were isolated from β cell–specific SNAP23-KO and SNAP23fl/fl
Control mice. Normal and T2D human islets were treated with control or SNAP23-shRNA adenovirus.
These lysate samples were separated in 12%–15% gradient SDS-PAGE, transferred to nitrocellulose membranes, and the separated proteins identified by the indicated primary antibodies. All blots were quantified
by densitometry scanning followed by analysis with Scion Image (release beta 4.0.2).
IPGTT, ITT, and glybenclamide treatment
Before and 2 weeks after AAV8-RIP1-Cre injection to knock out the β cell SNAP23 expression, the same
mouse was subjected to these tests.
IPGTT. IPGTT (2 g glucose/kg BW) was performed after an 18-hour fast. The mice were gently
restrained without anesthesia for blood collection from the tail vein for glucose determination by glucose
strips and insulin determination by RIA (EMD Millipore). The GK rats underwent IPGTT (2 g glucose/kg
BW) before infusion and 1, 2, 4, and 8 weeks postoperatively following an 18-hour fast.
ITT. ITT was conducted by i.p. injection of human biosynthetic insulin (1 U/kg BW, Eli Lilly Canada
Inc.) into mice or GK rats after a 6-hour fast; a drop of blood obtained from the tail vein at the indicated
times was used for glucose determination.
Glybenclamide treatment. GK rats had free access to food and water. Glybenclamide was suspended in
0.5% methylcellulose and was delivered by oral gavage at the optimal dose of 2 mg/kg (36, 53) and at a
volume of 10 mL/kg daily at 5 pm for 8 weeks. Methylcellulose (0.5%) was used as vehicle control, also at
a volume of 10 mL/kg (36, 53). Insulin at a dose of 1 U/kg BW was used for the ITT.
Islet morphometry
Pancreatic tissues from 4 βSNAP23-KO mice along with 4 littermate control SNAP23fl/fl mice or GK rats
were fixed for 24 hours in 4% paraformaldehyde in PBS (pH 7.4). Paraffin sections (7 μm thick) were
obtained separately from 3 levels of each pancreas (head, middle, and tail) and stained for insulin (Dako
Canada) (catalog IR00261-2). Insulin-immunostained sections were scanned using a Mirax Slide Scanner
(Carl Zeiss) and analyzed with Mirax Veiwer and software from 3DHISTECH. Insulin immunostaining
was calculated by an automated positive pixel count algorithm to determine total β cell area, islet number
per total pancreatic area, and islet size (6).
Insulin secretion
Perifusion assays on mouse pancreatic islets (50–70 islets per chamber) were performed as previously
reported (6, 14) to assess biphasic GSIS. Insulin secreted was determined by RIA. AUC was determined
above basal levels.
TIRF microscopy imaging
Exocytosis. The exocytosis study was performed with a Nikon TIRF microscopy system with a numerical
aperture 1.49 objective as previously reported (6, 8). To assess exocytosis, a monolayer of β cells was infected with Ad-NPY-EGFP, or alternatively with red-shifted NPY-tdmOrange2 (44) (gift from Sebastian Barg,
Uppsala University, Sweden) and cultured for 24 to 36 hours before performing TIRF microscopy. Fusion
events, indicated by abrupt brightening of NPY-EGFP (or –tdmOrange2) fluorescence, were manually
selected, analyzed, and categorized into predocked (docked on PM at basal, then fuses after stimulation) and
newcomer SGs (not predocked on PM at basal), the latter divided into short-dock (short residence time of
>200 milliseconds at the PM) or no-dock (<200 milliseconds at the PM, which is the interval of 1 frame).
Single-molecule imaging. For single-molecule imaging experiments, INS-832/13 cells seeded onto 25-mm
coverslips were transiently transfected with 0.2 μg plasmid DNA (SNAP25-mScarlet) with Lipofectamine
2000 (Life Technologies) for 3–5 hours before imaging to control the expression to a single-molecule level
as shown in Supplemental Figure 10. This TIRF microscopy system is built on a commercial inverted
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Olympus IX81 frame equipped with a UAPON 150× Oil NA 1.45 objective and Hamamatsu C9100-13
EM-CCD. Olympus cellTIRF software controlled the incident angles, and images were acquired by Volocity software. For the data analysis, the sum of SNAP25/Cav 1.2 binding time or events during the stimulation time was calculated, and then normalized to the number of Cav 1.2 clusters.
Generation of SNAP23-KO and -knockin INS-832/13. CRISPR/Cas9 SNAP23-KO and -knockin INS832/13 cells were generated according to the methods described by Sakuma et al. (54). After transfection
of gRNA and Cas9, single clones were picked up and seeded into 96-well plates. For characterization of
derived clones, PCR was used to preliminarily screen the targeted clones, amplifying the target site and
examine the band shifts, and Western blot was used to identify the derived clones. We used same clone-derived knockin/-out cell lines for imaging to minimize the heterogeneity. These CRISPR clones were also
verified by the coexpressed fluorophore mTagBFP2 or mNeonGreen as shown in Figure 9A. The sgRNA
target sites and success of this strategy are described in Supplemental Figure 9.
Structured illumination microscopy
This study was performed with the Zeiss Elyra PS1 microscopy system equipped with a 100× Oil NA 1.46
objective and Andor iX3 885 detector. Human β cells (control or SNAP23-KD condition) were stimulated
with 16.7 mM glucose plus 10 nM GLP-1 for 10 minutes and then fixed. Endogenous SNAP25 was labeled
with Alex Fluor 488 and Stx-1A/Stx-3 were labeled with Alex Fluor 647. Images were acquired by Zeiss
Zen 2012 software. Pearson’s correlation coefficient (PCC) calculation was performed with the Volocity
software (25). The PCC value varied from –1 to 1, whereby –1 means 100% no colocalization and 1 means
perfect colocalization.
Patch-clamp Cm measurements
This was performed as previously described (55). Patch electrodes were pulled from 1.5-mm thinwalled borosilicate glass, coated close to the tip with orthodontic wax (Butler), and polished to a tip
resistance of 2–3 MΩ when filled with intracellular solution. For measurement of Cm, the intracellular solution contained (in mM): 125 cesium glutamate, 10 CsCl, 10 NaCl, 1 MgCl2, 5 HEPES, 0.05
EGTA, 3 MgATP, and 0.1 cAMP (pH 7.2). The extracellular solution consisted of (in mM): 118 NaCl,
5.6 KCl, 1.2 MgCl2, 10 CaCl2, 20 tetraethylammonium chloride, 5 HEPES, and 5 D-glucose (pH 7.4).
Cm was estimated by the Lindau-Neher technique, implementing the Sine-DC feature of the Lock-in
module (40 mV peak-to-peak and a frequency of 1 kHz) in the whole-cell configuration. Recordings
were conducted using an EPC10 patch-clamp amplifier equipped with Pulse and X-chart software
programs (HEKA Electronik). Mouse and human β cells were identified using parameters previously
described (56, 57). Exocytic events were elicited by a train of ten 500-ms depolarization pulses from
–70 mV to 0 mV. All recordings were performed at 30°C.
Photolysis of caged Ca2+ and Cm measurements
Standard bath solution for the flash photolysis experiments contained (in mM): 138 NaCl, 5.6 KCl,
1.2 MgCl2, 2.6 CaCl2, 5 glucose, 5 HEPES (pH 7.4 with NaOH). Intracellular solution for flash experiments (in mM): 112 Cs-glutamate, 5 NP-EGTA, 3.7 CaCl2, 2 Mg-ATP, 0.3 Na2-GTP and 0.2 Mag-fura-2 (pH 7.2 with CsOH). NP-EGTA and Mag-fura-2 were purchased from Life Technologies. [Ca2+]i
was measured using Mag-fura-2 dye, which was excited with UV illumination alternating between 340
and 380 nm using a monochromator-based system TILL polychrome 5000 (TILL Photonics GmbH).
The ratio of the fluorescence signals was converted to [Ca2+]i using an in vivo calibration curve (30,
58). To obtain stepwise increases in [Ca2+]i, short flashes of UV light from a xenon arc flash lamp
(Rapp OptoElectronics) were applied to the whole cell. Cm was measured using an EPC-10 patchclamp amplifier controlled by the Lock-in module of PULSE software. The capacitance traces were
imported to IGOR Pro (WaveMetrics) for analysis.
Statistics
All data are presented either as means ± SEM with individual plots or as box-and-whisker plots. Statistical significance was assessed by repeated-measure ANOVA, or 2-tailed Student’s t test in SigmaStat
(Systat Software). Significant differences are indicated by asterisks. *P < 0.05; **P < 0.01; ***P < 0.001;
NS, not significant.
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Study approval
Animals were cared for and housed in the animal facility in the University of Toronto in accordance with
the Canadian Council on Animal Care Standards and the Animals for Research Act of Ontario. All animal
procedures were performed under appropriate project protocols that were approved by the University of
Toronto Faculties of Medicine and Pharmacy Animal Care and Ethics Committee. Human islets were
isolated and provided by IsletCore (P. MacDonald) of the University of Alberta, Canada, and their use was
approved by Institutional Review Board of the University of Toronto.
Author contributions
All the authors contributed to the conception and design of the study, acquisition of data (contributed most
by TL, TQ, and FK), and analysis and interpretation of data, as well as drafting of the article and revising
it critically for important intellectual content. All authors approved the final version to be published.
Acknowledgments
This work was supported by grants to HYG from the Canadian Institute of Health Research (MOP
86544 and PJT-159741). Some of the equipment used in this study was supported by the 3D (Diet,
Digestive Tract and Disease) Centre funded by the Canadian Foundation for Innovation and Ontario
Research Fund, project number 19442.
Address correspondence to: Herbert Y. Gaisano, Room 7342, Medical Sciences Building, 1 King’s College
Circle, Toronto, Ontario M5S 1A8, Canada. Phone: 416.978.1526; Email: herbert.gaisano@utoronto.ca.
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