16S rRNA Gene Sequencing

An aerobic, Gram-staining-variable, rod-shaped, endospore-forming and motile bacterial strain, designated CJ6 T , was isolated from a tidal flat on Ganghwa Island, South Korea. The isolate was characterized based on a polyphasic taxonomy approach. Strain CJ6 T grew optimally on R2A agar media at 30 C and pH 7. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CJ6 T belonged to the genus Paenibacillus, displaying the highest sequence similarity to Paenibacillus vulneris CCUG 53270 T (97.0 %) and clearly defined strain CJ6 T as a novel species within the genus. The G+C content of the genomic DNA was 49.9 mol%. The major polar lipid contents of strain CJ6 T were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified glycolipids. MK-7 was detected as the major respiratory quinone. The dominant fatty acid was anteiso-C 15 : 0. Analyses of phylogenetic, phenotypic, biochemical and chemotaxonomic characteristics indicated that strain CJ6 T was distinguishable from its closely related type strains. Therefore, strain CJ6 T represents a novel species in the genus Paenibacillus, for which name Paenibacillus limicola sp. nov. is proposed; the type strain is CJ6 T (=KACC 19303 T =JCM 32079 T). The genus Paenibacillus was first proposed by Ash et al. [1] as a member of the 'group 3 bacilli' within the genus Bacillus with 11 Bacillus species. At the time of writing, the genus contains more than 213 novel species with validly published names (www.bacterio.net/paenibacillus.html). Typically, members of this genus are either facultatively anaerobic or strictly aerobic, rod-shaped, comprise anteiso-C 15 : 0 as the major fatty acid, and show a range of the G+C content from 39 to 54 mol% [2]. The purpose of the present study was to establish the taxonomic position of the novel Paenibacillus-like strain CJ6 T based on polyphasic taxonomy. Strain CJ6 T was isolated from the tidal flat of Ganghwa Island (37 36¢ 58.40 † N 126 22¢ 37.40 † E), South Korea through a standard dilution plating technique on R2A agar (BD) media. The strain was routinely cultivated on R2A at 30 C for 3 days and preserved at À80 C with 30 % (w/v) glycerol solution.