ADP

Le référendum d’initiative partagée (RIP) initié aux fins de faire obstacle à la privatisation d’Aéroports de Paris (ADP) peut être salué comme un progrès attendu de la démocratie participative, mais soulève certaines interrogations au regard de ses conséquences institutionnelles.

The study which was conducted in Umuahia Agricultural Zone of Abia State, Nigeria made use of the EAs in the Zone in identifying the contact – farmers. A simple random method was used to select 20 % of the contact farmers which gave a sample size of 144 respondents that was used. Structured questionnaire was used in the generation of the primary data. Descriptive statistics were used in the analysis of the data. Results showed 69.4 % of the respondents were males, and 30.6 % females. About 83.3 % were married with a mean age of 43 years and 93.1 % had formal education with a mean household of 6 persons, and 13.5 years, of mean years of farming experience with a mean farm-size of 0.9 hectares and mean monthly income of ₦33,569.44 respectively. A high level (X = 2.68) of participation in ADP – capacity building programmes was recorded. The respondents, were within the categories of early adopters (X = 3.68) in the adoption of ADP recommended innovations. About (X = 69.4 %) of the respondents indicated that, the ADP trainings did not address their felt needs, (X = 62.5 %) sessions were held at the wrong time, (X = 58.3%) training centres not centralized and (X= 51.4%) felt that training sessions were too long & boring. The study recommends that the ADP should strengthen the feedback mechanism as to ensure that their trainings address the felt needs of farmers. Also, a performance evaluation of the contact farmers by the ADP should be a regular exercise as to ensure that it is only the performing ones that are

ADP plays a key role in platelet aggregation which has led to the development of antiplatelet drugs that target the P2Y 12 receptor. The aim of this study was to characterize the effects of two novel P2Y 12 receptor antagonists, BX 667 and its active metabolite BX 048, on ...

The article was published on the Asia Designer Communication Platform (ADP) website
Article Title: A Brief Discussion on Object-Process Methodology (OPM) and Design Thinking Approach
Publishing Date: October 24, 2019
Article Author: Sheng-Hung Lee
Article Link: https://www.ad-p.org/news_content.php?id=347

Ceramidases (CDase) are enzymes that catalyze the hydrolysis of N-acyl linkage of ceramide (Cer) to generate sphingosine and free fatty acids. In this study we report the purification and characterization of a novel second type of neutral ceramidase from rat brain (RBCDase II). Triton X-100 protein extract from rat brain membrane was purified sequentially using Q-Sepharose, HiLoad16/60 Superdex 200pg, heparin-Sepharose,

A number of new nitric oxide (NO)-precursors were synthesized by grafting nitrate-containing moieties on the structures of the benzyloxy isonipecotanilide derivatives 1 and 2 already reported as moderately potent antiplatelet agents. Various nitrooxy (ONO2)-alkyl side chains were covalently linked to the piperidine nitrogen of the parent compounds through carbamate and amide linkage, and the synthesis of a benzyl nitrate analog (15) of compound 1 was also achieved. The in vitro vasodilatory activities, as well as platelet anti-aggregatory effects, of the newly synthesized organic nitrates were assessed. The (ONO2)methyl carbamate-based derivative 5a and the benzyl nitrate analog 15, which on the other hand retain activity as inhibitors of ADP-induced platelet aggregation, exhibited strong NO-mediated vasodilatory effects on pre-contracted rat aorta strips, with EC50 values in the low nanomolar range (13 and 29nM, respectively). Experiments carried out with the selectively inhibited ...

Eight protein crystal structures, which have been refined against X-ray diffraction data extending to atomic resolution, 1.2 A or better, were inspected using four different validation tools, PROCHECK, PROVE, SQUID and WHATCHECK. Two general questions were addressed. (1) Do the structures imply changes in "expected" stereochemical properties and are the target values used for restraints in the validation programs and the refinement protocol appropriate? (2) Can errors in models be detected and how reliable are the coordinates after refinement? Preliminary analysis by members of the network led to modifications both to the validation programs and to the refinement protocols. The results of the final analyses are reported here. Apparent discrepancies in cell dimensions were identified. Most stereochemical properties are shown to be more tightly clustered than for lower resolution analyses. In contrast the omega angle has a wider distribution. The validation software is gener...

In this work, a combined experimental and theoretical study on molecular structure, vibrational spectra of 1-amino-2,6-dimethylpiperidine (ADP) has been reported. The FTIR and FT-Raman spectrum have been recorded in the region 4000-400 cm -1 and 3500-50 cm -1 , respectively. The molecular geometry, harmonic vibrational frequencies and bonding features of ADP have been calculated by using ab initio /HF and density functional theory/B3LYP methods with 6-31+G(d,p) basis set. A detailed interpretation of the FTIR, FT-Raman, NMR spectra of ADP has also been reported. Natural bond orbital analysis has been carried out to explain the charge transfer or delocalization of charge due to the intra-molecular interactions. Energy of the highest occupied molecular (HOMO) orbital and lowest unoccupied (LUMO) molecular orbital have been predicted.

Lysophosphatidic acid (LPA) and sphin- gosine-1-phosphate (S1P) are agonists of the endothelial differentiation gene (Edg) family of G-protein-coupled receptors. LPA and S1P are generated by platelet activation during blood coagulation. Both lipids induce assembly of exogenous fi- bronectin (FN) by fibroblasts. This study examined whether LPA and S1P stimulate binding and assembly of fluoresceinated FN (FITC-FN) by adherent platelets. LPA

NTPDase is one of the principal enzymes involved in the sequential hydrolysis of ATP. In the present study, the presence and functionality of NTPDase in the mesenteric vein and artery were examined. Adenosine triphosphate (ATP) (0.01–1000 pmol) induces a dose-dependent vasodilation in the isolated arterial and venous mesenteric vasculatures of the guinea pig. Adenosine diphosphate (ADP) (0.01–1000 pmol) but not adenosine monophosphate (AMP) (0.01–1000 pmol) induces a similar response in the mesenteric vascular circuit. L-NAME, a nitric oxide synthase inhibitor (200 µM, 30 min), significantly reduces the arterial dilatory effect of ATP and abolishes the responses to ADP and AMP. Complete removal of the endothelium with 3-[(3-cholamidopropyl) dimethylammonio]-1-propansulfonate (CHAPS) (20 mM, 2 × 45 s) abolishes ATP-induced responses. Infusion of ATP in the vascular circuit generated detectable amounts of ADP and AMP, as measured by HPLC. CHAPS treatment significantly reduced the leve...

SummaryP2Y12, one of the two platelet receptors for adenosine diphosphate (ADP), plays a central role in platelet function. Defects of P2Y12 should be suspected when ADP, even at high concentrations (≥10 μM), is unable to induce full, irreversible platelet aggregation. Patients with congenital P2Y12 defects display a mild-to-moderate bleeding diathesis of variable severity, characterised by mucocutaneous bleeding and excessive post-surgical and post-traumatic blood loss. Drugs that inhibit P2Y12 are potent antithrombotic drugs, attesting the central role played by P2Y12 in platelet thrombus formation. Clopidogrel, the most widely used drug that inhibits P2Y12, is effective both in monotherapy and in combination with acetylsalicylic acid (ASA). Its most important drawback is the inability to inhibit adequately P2Y12-dependent platelet function in about 1/3 of patients, at the recommended therapeutic doses. The incidence of bleeding events is similar in ASA-treated and clopidogrel-tre...

The interactions of nucleotides at the allosteric and catalytic sites of phosphorylase kinase were examined. Binding of nucleoside triphosphates at the nucleoside diphosphate allosteric activation site inhibited enzymatic activity; this was observed with either ATP or GTP. Increasing concentrations of ADP caused a biphasic response: low concentrations activated and higher concentrations inhibited. Inhibition was due to the binding of ADP at the catalytic site, as opposed to an allosteric inhibitory site. GDP activated at low concentrations, but did not inhibit even at relatively high concentrations, and is therefore a specific probe for the allosteric site. Maximal activity of the nonactivated holoenzyme at pH 6.8 is achieved at an optimal ratio of ATP to ADP, such that the inhibitory actions of ATP at the allosteric site and of ADP at the catalytic site are balanced. Various potential molecular mechanisms to explain the allosteric activation by ADP were examined and ruled out, thus...