Antiproliferative

Magnetic spinel ferrite nanoparticles possess high scientific attention for the researchers attributed to its broad area for biomedicine purposes, comprising cancer magnetic hyperthermia and targeted drug delivery. Herein, we report the ultrasound irritation assisted the sol-gel method for the spinel Zn 0.5 Co 0.5-x Ag 2x Fe 2 O 4 (x = 0.00, 0.10, 0.20, and 0.30) nanoparticles (NPs) synthesis. The Rietveld refinement patterns revealed the successful synthesis of the cubic structure Zn 0.5 Co 0.5-x Ag 2x Fe 2 O 4 and the crystallite size ranged from 14.1 nm to 18.4 nm. Also, the optical band gap decreased from 2.39 eV for Co-Zn ferrite to 2.20 eV for Zn 0.5 Co 0.2 Ag 0.6 Fe 2 O 4 sample. Electron paramagnetic resonance spectroscopy was used to study ferromagnetic resonance characteristics of the Zn 0.5 Co 0.5-x Ag 2x Fe 2 O 4 samples. The resonance field was increased from 2512.2 Gauss for Co-Zn ferrite sample to 2834.8 Gauss for Zn 0.5 Co 0.2 Ag 0.6 Fe 2 O 4 sample, while the line width decreased from 2401.3 to 1990.1 Gauss. Also, the saturation was reduced from 45.68 emu.g-1 for the pristine Zn 0.5 Co 0.5 Fe 2 O 4 sample to 22.20 emu.g-1 for Zn 0.5 Co 0.2 Ag 0.6 Fe 2 O 4 sample. Furthermore, cytotoxicity of Zn 0.5-Co 0.2 Ag 0.6 Fe 2 O 4 NPs against human breast cancer MCF-7, HepG2 liver cancer, and LoVo colorectal cancer cells was examined. The cytotoxicity of Zn 0.5 Co 0.2-Ag 0.6 Fe 2 O 4 on the previous cancer cell lines resulted in inhibition of cell growth estimated by MTT assay. The exposure of MCF-7, HepG2, and LoVo cancer cells

Novel substituted pyrazolo[3,4-d]pyrimidines 4-27 were synthesized starting with 1-substituted-5-amino-1Hpyrazole- 4-carbonitrile 1. Some of the prepared compounds were tested for their anticancer as well as antioxidant activities. As anticancer agents, the tested compounds were evaluated for their antiproliferative potency and their effects on the free-radical-metabolizing enzymes including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). The levels of the oxidative stress parameters including hydrogen peroxide (H2O2), nitric oxide (NO) and reduced glutathione (GSH) were also investigated in vitro on human breast (MCF-7), liver (HepG2) and lung (A549) cancer cell lines comparing with the reference anticancer drug cisplatin. The antioxidant ability of the tested compounds was measured by evaluating their radical scavenging ability (RSA%) using DPPH and ABTS radical scavenging assays. Results indicated that most of the compounds exhibited remarkable cyt...

Breast cancer is the second main cause of death among women. The use of medicinal plants has been common in many countries since ancient times. The aim of this study was to evaluate the antioxidant and anti-proliferative potential of Achillea millefolium L. The antioxidant activity of the aqueous and hydroalcoholic extracts of Achillea millefolium L. leaf and flower was measured by DPPH and FRAP method while its anti-proliferative activity on MCF-7 human breast cancer cell line was evaluated using MTT assay. The ethanolic extract of the leaf and the methanolic extract of the flower had the highest radical scavenging and ferric ion reducing activities. Time and dose-dependent cytotoxic effects of different extracts were observed on MCF-7 cells. The difference in cell viability between hydroalcholic (methanol and ethanol) and aqueous extracts of leaf and flower was significant (P< 0.05), but there was no significant difference in cell viability between methanolic and ethanolic extracts of leaf (P< 0.05). IC50 values varied between 7 and 93 μg/ ml with ethanolic extracts being more cytotoxic and flower extract exhibiting a higher antiproliferative effect than leaf extract. The presence of antioxidant activity as well as high cytotoxic effect of all examined extracts suggest that Achillea millefolium may possess a potential chemotherapeutic activity for breast cancer treatment.

This study investigated the antioxidant, immunomodulatory and antiproliferative potentials of gelatin hydrolysates from seabass skins in cell model systems. Gelatin hydrolysates were extracted from seabass skins using different processes and enzyme concentrations. The ability of the hydrolysates to protect against H 2 O 2-induced DNA damage was assessed on U937 cells using the Comet assay, and one of the samples showed DNA protective effects. All samples showed immunomodulatory potential by significantly (P < 0.05) reducing interleukin-6 (IL-6) and IL-1b production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Antiproliferative activities of seabass skin hydrolysates were measured using human colon cancer (Caco-2) and liver cancer (HepG2) cell lines as the model cell cultures. The inhibition of cell proliferation of Caco-2 and HepG2 cancer cells occurred in a dose-dependent manner at concentrations of 1–25 mg mL À1. Therefore, seabass skin hydrolysates prepared using an appropriate process could serve as a potential functional food ingredient with various health benefits.

The aim of the present study was to assess the effect of the aqueous extracts of Psidium guajava and Piper betle plants on the proliferation of cancerous cell lines, that is, KB and HeLa cell line. Using the neutral red cytotoxicity assay, the IC(50) of P. guajava and P. betle were determined at 29.0 +/- 0.4 and 29.5 +/- 0.3 mu g/ml, respectively, indicating both plant extracts equally potent for the treatment of cancerous oral epidermal lesions. However, a less potent anti-proliferative activity was recorded by P. guajava towards HeLa cell line with an IC(50) of 51.0 +/- 0.6 mu g/ml, whereas P. betle extract did not affect the proliferation of HeLa.

We report the synthesis, characterization, and biological properties of chitosan nanoaggregates from gladius of squid, Uroteuthis duvauceli. í µí»½-Chitin extracted from gladius was deacetylated to chitosan and further reduced to nanosize using ionic gelation process. The morphology and occurrence of chitosan nanoaggregates (CSNA) were observed using transmission electron microscopy (TEM). The degree of deacetylation (DD%) calculated from Fourier transform infrared (FTIR) spectrum showed high value (∼94 ± 1.25%) for chitosan. The CSNA depicts low molecular weight, stable positive zeta potential, and less ash and moisture content with high water and fat binding capacity. The antimicrobial activity was tested against pathogenic microorganisms, which depicted significant rate of inhibition against Staphylococcus aureus and Escherichia coli due to high cellular uptake. The antioxidant analysis for CSNA demonstrated high reducing power and scavenging activity towards superoxide radicals compared with the commercially available chitosan. Furthermore, nanoaggregates exhibited low cytotoxic behavior in biological in vitro tests performed using cervical cancer cell line. These results indicate that chitosan nanoaggregates synthesized from waste gladius will be highly efficient and safe candidate for biological applications as food packing film, drug carrier, and tissue engineering.

Hepatocellular carcinoma (HCC) is the fifth most common forms of cancer occurring worldwide and its incidence is on the rise. The treatment options available currently have certain limitations and research is on for discovering new molecules with a better outcome profile. Bioactive compounds from plants have shown promise in the treatment of HCC. Flavonoids are plant secondary metabolites and diets rich in these have been shown to decrease the incidence of various forms of cancer. Several studies have indicated the anticancer ability of various flavonoids to act by various mechanisms. In the present study  three dimethoxy flavones viz. 3,6-dimethoxy flavone (3,6-DMF), 6,2'-dimethoxy flavone (6,2'-DMF) and 6,3'-dimethoxy flavone (6,3'-DMF) were evaluated for anti proliferative activity and apoptosis inducing ability against HepG2 cell line. All the three compounds have shown dose dependent anti proliferative activity at the doses of 50, 100, 150,200 and 250 ìg/ml and the IC50 values  were 200 ìg/ml for 3,6-DMF and 6,2'-DMF and for 6,3'-DMF the value was 150 ìg/ml at 48h. The compounds were subjected to flow cytometry and it was seen that the three compounds caused an increase in the number of cells in the G1 phase. All the 3 compounds were shown to promote apoptosis as was seen in the nuclear morphological examination study using Propidium iodide staining and DNA fragmentation by the agar gel electrophoresis technique. Our study has shown that the three dihydroxy flavones may hold promise for development as a drug of use in the treatment of Hepatocellular carcinoma.

Chitosan was modified into nanoparticle chitosan in order to increase its absorptivity and solubility in water so that it can be applied in various fields. The objective of this research was to produce nanometer-sized chitosan, to characterizethe functional groups and the particle size of chitosan as well as to test its antimicrobial activity. Chitosan nanoparticles were synthesized through ionic gelation method by reacting chitosan with tripolyphosphate ions through ionic crosslinking reaction. The functional groups of chitosan were determined from its FTIR spectrum, the particle size was obtained from its X-ray diffractogram and particle size analysis (PSA). Chitosan nanoparticles with the smallest size based on XRD wereobtained at a concentration of 0.1% chitosan (CNPs 1) and chitosan 0.2% (CNPs 2) with the addition of 0.1 % tripolyphosphate ion. The sizes of chitosan nanoparticles,CNPs 1 and CNPs 2,obtained from the analysis of PSA were 224.68 and 204.32 nm, respectively. Polydi...