This work covers peculiarities of introduction of the hybrid form of hyacinth (Hyacinthus) introduced and spread in Georgia into in-vitro culture and induction of embryogenesis. Aiming to derive the sterile cultures, we matched the... more
This work covers peculiarities of introduction of the hybrid form of hyacinth (Hyacinthus) introduced and spread in Georgia into in-vitro culture and induction of embryogenesis. Aiming to derive the sterile cultures, we matched the sterilizing agent and its exposition. Besides, we used hyacinth bulb peel as an initial explant to derive the embryogenic callus. Aiming induction of callusogenesis and embryogenesis, we tested and researched concurrent exposition to benzylaminopurine and auxins (NAA and IBA) in concentrations correlation of 2:10; 2:15; 2:20. We derived the embryogenic calli and somatic embryoids, developed conditions for maturation of the embryoids and regeneration of the somatic embryos.
The effect of plant growth regulators on in vitro propagation of Fragaria x ananassa Duch. cv. Pharachatan 80 was conducted in sterile conditions. For shoot multiplication, shoot tip were cultured on Murashige and Skoog (MS, 1962) medium... more
The effect of plant growth regulators on in vitro propagation of Fragaria x ananassa Duch. cv. Pharachatan 80 was conducted in sterile conditions. For shoot multiplication, shoot tip were cultured on Murashige and Skoog (MS, 1962) medium for 6 weeks. The results indicated that shoot tips could induce shoots and roots 90%. Then shoot tips were cultured on MS medium supplemented with combination of cytokinin (BAP or Kinetin at 0, 0.1, 0.2 and 0.5 mg /l) and auxin (IBA at 0, 0.05, 0.1 and 0.2 mg/l) for 6 weeks. The results showed that shoot tips cultured on MS medium supplemented with 0.2 mg/l BAP and 0.2 mg/l IBA had the highest number of shoots (1.70 ± 0.82 shoots/explant) and shoots tips that cultured on MS medium supplemented with 0.2 mg/l IBA had the highest number of roots (5.40 ± 4.67 root/explant). From this study, it was found that MS medium supplemented with BAP and IBA gave the best results on shoot and root induction than MS medium supplemented with Kinetin and IBA. For the...
Background: C. cretica is a medicinal and ornamental halophytic plant which used for alterative, anthelmintic, tonic and aphrodisiac purposes. The purpose of this study was to detect the quercetin content in C. cretica and to evaluate the... more
Background: C. cretica is a medicinal and ornamental halophytic plant which used for alterative, anthelmintic, tonic and aphrodisiac purposes. The purpose of this study was to detect the quercetin content in C. cretica and to evaluate the effects of exogenous application of abscisic acid and cytokinin hormones on FLS gene expression. Methods: Abscisic acid and cytokinin (BAP) was sprayed in two-stages (8 days intervals) with two different concentration of 300 and 500 ppm. Quercetin content was confirmed by HPLC analysis in aerial parts of plant. Results: The findings revealed that gene expression of FLS increased in the second stage of abscisic acid treatment. Observation showed significant differences in gene expression between treated and control samples, at level of 1%. In the first phase, there were no significant differences between the abscisic acid treated and control samples. In addition, the cytokinin treatment (500 ppm) resulted to the expression of the FLS gene at 5% level, which implied that cytokinin affected the FLS gene expression. Conclusions: In this research, it was proved that the dihydroquercetin was present in C. cretica which changed to quercetin through the flavonol synthase enzyme.
