IOSR Journal of Biotechnology and Biochemistry

Abstract: This paper proposes modeling of canine cardiac action potential using fractional differential equations (FDE). The movement of ions across cardiac myocytes (heart muscle) generates a voltage signal which propagates along the body of the cell as action potential. The complex behavior of such physiological events can be understood by developing mathematical models that describe the biological phenomena. Typically, ordinary linear differential equations are used for constructing these mathematical models. In this paper we have shown that a non-integer order differential equation model can capture these events more precisely. Using FDE we are able to model cardiac action potential with a higher degree of accuracy compared to ordinary differential equations (ODE). Keywords: Action potential (AP), Cardiac arrhythmia, Cardiac myocytes, Electrical model, Fractional differential equations (FDE), Ion exchange mechanism

Abstract: Considering the constraints due to unavailability of uniform quality chicken manure in local market, three different combinations of wheat and mustard meal (de-oiled cake) were tested for their ability to affect yield and cost of production of Button Mushroom (Agaricus bisporus). Compost was prepared by mixing 18, 22 and 25% mustard meal with wheat straw by short method of composting. 80% chicken manure with wheat straw was the control. All the ingredients composted for 18 days of two phases composting using tunnel for peak heating as advocated by Shandilya et al, 1976, fresh grain spawn was obtained from Bharat Mushrooms, Delhi, India. Formaldehyde treated casing soil (2:1 mixture of 2 year old farm yard manure and garden soil) was used for casing of beds. The results indicate that all the combinations affected yield and proved to be promising factors for low cost production of mushroom. Combination with 22% mustard meal gave the maximum production of Mushroom (226 Kg/ton of compost). Subject: Basic science Keywords: Mustard meal, Agaricus bisporous, Compost

Occurrence of somaclonal variants is a common and persistent feature of TC industry. The somaclonal variants are important source of genetic variation in asexually propagated plant species and thus are considered as a potential mean for development of new crop variety. Present study showed identification of DNA markers (SSR) linked to off-type traits in micropropagated banana. For which three category of off-type plants (O1,O2 & O3) were characterized and used for DNA isolation. A total of 150 SSR and ISSR markers were screened, all one hundred fifty SSR markers showed monomorphic banding pattern. Out of these markers only nine of them were screened and selected for CAPS marking, out of which only one marker MaCIR112 showed slight polymorphism. The PCR product of the same marker was then digested using restriction enzyme, and only Pst I showed polymorphic bands between off-types and normal plants.Digested product of MaCIR112 and some undigested product of off-types were then sequenc...

Dendrobium fimbriatum var.oculatum is an epiphytic orchid of of immense floricultural apppeal as well as some local medicinal values. It is a native to north-eastern India and parts of Indo-Chinese peninsula with a height of about 1m bearing beautiful bunches of bright yellow flowers having a reddish brown blotch in the middle and reddish streaks at the base, mildly scented and flowers lasting for 8-10 days. Like many other orchids of this region, this species is also found to be in the verge of extinction, due to large-scale denundation of forest areas. So in order to preserve it from possible extinction, attempts are made to multiply it through in vitro culture of nodular portions. NAA (1 mg/l) in MS medium was found to be most effective for early initiation of bud and multiple shoot induction.Full strength liquid MS medium proved to be the best for nodal explant culture.