We used radiotelemetry during 2004 and 2005 to investigate seasonal movements and habitat use and to improve our understanding of how land management practices were affecting Western Massasauga (Sistrurus tergeminus) populations in... more
We used radiotelemetry during 2004 and 2005 to investigate seasonal movements and habitat use and to improve our understanding of how land management practices were affecting Western Massasauga (Sistrurus tergeminus) populations in Nebraska. We found Western Massasaugas to brumate in crayfish burrows 100% of the time, and spring emergence occurred during March-April. The longest movements occurred during May when snakes were moving away from brumation sites to summer habitat, after which movements decreased until a second peak in August. We did not find significant differences between male and female activity area, range length, total distance moved, daily movement, or distance from spring and fall brumation sites. However the core activity range of females was significantly less than that of males. During the active season, grassland habitat was used 75.6% of the time compared to habitats of grassland-shrubs (10.7%), hydrophilic vegetation (9.1%), woodlands (3.1%), and shrublands (1.5%). Identifying variations in a species spatial ecology is important for achieving long-term conservation goals because life history events, resource availability, and land management practices may have an effect on seasonal movements and habitat use. The results of our study demonstrate the need for, and some of the necessary data for, improved land management planning in order to meet conservation needs of the Western Massasauga in Nebraska.
The protein composition of the venoms of the three subspecies of Sistrurus catenatus (S. c. catenatus, tergeminus, and edwardsii) and a basal species, Sistrurus miliarius barbouri, were analyzed by RPHPLC, N-terminal sequencing, MALDI-TOF... more
The protein composition of the venoms of the three subspecies of Sistrurus catenatus (S. c. catenatus, tergeminus, and edwardsii) and a basal species, Sistrurus miliarius barbouri, were analyzed by RPHPLC, N-terminal sequencing, MALDI-TOF peptide mass fingerprinting, and CID-MS/MS. The venoms of the four Sistrurus taxa contain proteins from 11 families. The protein family profile and the relative abundance of each protein group in the different venoms are not conserved. Myotoxins and 2-chain PLA2s were detected only in S.c. catenatus and S.c. tergeminus, whereas C-type BPP and Kunitz-type inhibitors were exclusively found in S.c. edwardsii and Sistrurus miliarius barbouri. Among major protein families, taxa were most similar in their metalloproteases (protein similarity coefficient value: 34%) and most divergent in PLA2s (12%), with values for disintegrins and serine proteases lying between these extremes (25 and 20%, respectively). The patterns of venom diversity points to either a gain in complexity in S. catenatus taxa or a loss of venom diversity occurring early on in the evolution of the group involving the lineage connecting S. milarius to the other taxa. The high degree of differentiation in the venom proteome among recently evolved congeneric taxa emphasizes the uniqueness of the venom composition of even closely related species that have different diets. Comparative proteomic analysis of Sistrurus venoms provides a comprehensive catalog of secreted proteins, which may contribute to a deeper understanding of the biology and ecology of these North American snakes and may also serve as a starting point for studying structure-function correlations of individual toxins.
We used radiotelemetry during 2004 and 2005 to investigate seasonal movements and habitat use and to improve our understanding of how land management practices were affecting Western Massasauga (Sistrurus tergeminus) populations in... more
We used radiotelemetry during 2004 and 2005 to investigate seasonal movements and habitat use and to improve our understanding of how land management practices were affecting Western Massasauga (Sistrurus tergeminus) populations in Nebraska. We found Western Massasaugas to brumate in crayfish burrows 100% of the time, and spring emergence occurred during March April. The longest movements occurred during May when snakes were moving away from brumation sites to summer habitat, after which movements decreased until a second peak in August. We did not find significant differences between male and female activity area, range length, total distance moved, daily movement, or distance from spring and fall brumation sites. However the core activity range of females was significantly less than that of males. During the active season, grassland habitat was used 75.6% of the time compared to habitats of grassland-shrubs (10.7%), hydrophilic vegetation (9.1%), woodlands (3.1%), and shrublands (...
