Los estudios sobre accidentología vial muestran preocupantes estadísticas de mortalidad y daños materiales. Mediante los mismos se busca establecer medidas preventivas a través del estudio de las causas. Pero en diversas ocasiones no es... more
Los estudios sobre accidentología vial muestran preocupantes estadísticas de mortalidad y daños materiales. Mediante los mismos se busca establecer medidas preventivas a través del estudio de las causas. Pero en diversas ocasiones no es posible tipificar las causas, porque se desconocen.
Por otro lado, los distintos modelos de percepción del riesgo fundamentan medidas de seguridad vial a fin de disminuir el riesgo asociado al tránsito, en general, centradas en la anticipación de los factores de riesgo o en el aspecto motivacional de la percepción del mismo.
Nos proponemos diseñar y construir dispositivos y sistemas que permitan la detección y registro de los factores de accidentes de tránsito en rutas, basados en aplicaciones móviles de sistemas de información geográfica (SIG), como un aporte a la seguridad vial, mediante medidas fundamentadas en los distintos modelos de percepción del riesgo.
MAPK Pmk1p is the central element of a cascade involved in the maintenance of cell integrity and other functions in Schizosaccharomyces pombe. Pmk1p becomes activated by multiple stressing situations and also during cell separation.... more
MAPK Pmk1p is the central element of a cascade involved in the maintenance of cell integrity and other functions in Schizosaccharomyces pombe. Pmk1p becomes activated by multiple stressing situations and also during cell separation. GTPase Rho2p acts upstream of the protein kinase C homolog Pck2p to activate the Pmk1 signalling pathway through direct interaction with MAPKKK Mkh1p. In this work we analyzed the functional significance of both Rho2p and Pck2p in the transduction of various stress signals by the cell integrity pathway. The results indicate that basal Pmk1p activity can be positively regulated by alternative mechanisms which are independent on the control by Rho2p and/or Pck2p. Unexpectedly, Pck1p, another protein kinase C homolog, negatively modulates Pmk1p basal activity by an unknown mechanism. Moreover, different elements appear to regulate the stress-induced activation of Pmk1p depending on the nature of the triggering stimuli. Whereas Pmk1p activation induced by hyper-or hypotonic stresses is channeled through Rho2p-Pck2p, other stressors, like glucose deprivation or cell wall disturbance, are transduced via other pathways in addition to that of Rho2p-Pck2p. On the contrary, Pmk1p activation observed during cell separation or after treatment with hydrogen peroxide does not involve Rho2p-Pck2p. Finally, Pck2p function is critical to maintain a Pmk1p basal activity that allows Pmk1p activation induced by heat stress. These data demonstrate the existence of a complex signalling network modulating Pmk1p activation in response to a variety of stresses in fission yeast.
