Vitexin
0 Followers
Recent papers in Vitexin
CHAPTER 3 - Materials and Methods
3.1 Introduction
3.2 Sample Preparation
3.3 Extraction of Vitexin and Isovitexin from Ficus Deltoidea Leaves
3.4 Method of Analysis
3.1 Introduction
3.2 Sample Preparation
3.3 Extraction of Vitexin and Isovitexin from Ficus Deltoidea Leaves
3.4 Method of Analysis
Mas Cotek or Ficus deltoidea is regarded as one of the most precious herbal plants in Malaysia due to its content of beneficial active compounds and appreciation as a traditional remedy. This research investigated performance of aqueous... more
Mas Cotek or Ficus deltoidea is regarded as one of the most precious herbal plants in Malaysia due to its content of beneficial active compounds and appreciation as a traditional remedy. This research investigated performance of aqueous or water extraction of dried Mas Cotek leaves for vitexin and isovitexin compounds using three regiments of temperatures (50, 70 and 100°C) in sample-to-water ratios of 1:10, 1:20 and 1:30g/mL during 8 hours of extraction. The optimum yield recorded for vitexin compound was 0.463 ± 0.045 (%w/w) from; 1:30 g/mL sampleto-water ratio, 50°C temperature at 4th hour of extraction. While for isovitexin compound, the optimum value recorded was 0.136 ± 0.015 (%w/w) from; 1:20 (g/mL) sample-to-water ratio, 50°C temperature at 5th hour of extraction. The morphological characterization of the extracted dried leaves particles performed using FE-SEM showed an intact and less damaged surface of extracted sample after 8 hours of extraction. The experimental values u...
Ficus deltoidea or Mas cotek is one of the most popular plant herbs that have been widely used traditionally as postpartum treatment and health tonic. Preliminary or existing extraction method has been used to extract the plant herbs that... more
Ficus deltoidea or Mas cotek is one of the most popular plant herbs that have been widely used traditionally as postpartum treatment and health tonic. Preliminary or existing extraction method has been used to extract the plant herbs that have low productivity range. This method however can still produce the desired products, but the work can be laborious and not efficient in large scale processing. Besides, the sample preparation has no evidence in advances because obtaining such extraction products at sufficient concentrations usually involves extraction with large amounts of organic solvents and toxic, followed by evaporation. Production of Vitexin from Ficus deltoidea extraction under various sonication regiments is reported. Batch extractions were carried at low intensity sonication (8.66 W cm-2 sonication intensity at the sonotrode tip) using 10%, 20% and 40% duty cycles. (A duty cycle of 10%, for example, was equivalent to sonication for 1 s followed by a rest period (no sonication) of 10 s.) The extracts were compared with those obtained by conventional boiling extraction, in terms of bioactive constituents yield and chemical composition. In the aqueous (conventional) extracts, the actual percentage weight of Vitexin varied in the range 0.122 ± 0.013 ~ 0.386 ± 0.018 (%w/w) compared to sonicated extracts with 2-fold increases at a range of 0.127 ± 0.001 ~ 0.738 ± 0.050 (%w/w) after 8 hours of extraction with sample-to-water ratio of 10:1 (g/ml) at 50, 70 and 100°C. Both ultrasonic-assisted and aqueous extracts were characterized and standardized by HPLC-diode array detector using a pharmacologically active marker, Vitexin. The experimental values under optimal conditions were in good consistent with the predicted values, which suggested that ultrasonic-assisted extraction (UAE) is more efficient process as compared to conventional boiling extraction (AE). It recommends that ultrasound extraction of F. deltoidea leaves is substantially feasible to replace the traditional time-consuming and low efficiency preparation procedure in the future modernized and commercialized manufacture of this highly valuable herbal medicine.
Developing a new agent in the anti-inflammatory and analgesic field, plants secondary metabolites can be a good source for the Non-Steroidal Anti-inflammatory Drugs (NSAID) drug development. For this purpose we subjected the active... more
Developing a new agent in the anti-inflammatory and analgesic field, plants secondary metabolites can be a good
source for the Non-Steroidal Anti-inflammatory Drugs (NSAID) drug development. For this purpose we
subjected the active compounds of Mimosa pudica Linn. to reveal its potentiality by molecular docking analysis
to find out its potent compound against COX which was done by GOLD docking analysis. Docking studies by
GOLD showed that vitexin of Mimosa pudica had the highest fitness score against the COX-1 which is 60.43 and
63.49 for COX-2 enzyme. Vitexin of Mimosa pudica detected with significant fitness score and hydrogen
bonding against COX-1 and COX-2 which may be a potent analgesic compound.
source for the Non-Steroidal Anti-inflammatory Drugs (NSAID) drug development. For this purpose we
subjected the active compounds of Mimosa pudica Linn. to reveal its potentiality by molecular docking analysis
to find out its potent compound against COX which was done by GOLD docking analysis. Docking studies by
GOLD showed that vitexin of Mimosa pudica had the highest fitness score against the COX-1 which is 60.43 and
63.49 for COX-2 enzyme. Vitexin of Mimosa pudica detected with significant fitness score and hydrogen
bonding against COX-1 and COX-2 which may be a potent analgesic compound.
Vitexin is an apigenin flavone glycoside isolated from natural sources. It is considered as important as other flavonoids such as quercetin, rutin, kaempferol. In this review, we have documented some major occurrence and its major... more
Vitexin is an apigenin flavone glycoside isolated from natural sources. It is considered as important as other flavonoids such as quercetin, rutin, kaempferol. In this review, we have documented some major occurrence and its major pharmacological activities such as anti-inflammatory, anti-oxidant, cardioprotective, anti-cancer, antinociceptive, anti-convulsant, memory enhancing potential and anti-diabetic activities. We have also drawn its 3D structure,total charge density by huckel using ChemDraw 3D and its theoretical chemical properties and analysis by ChemDraw.
Mas Cotek or Ficus deltoidea var. deltoidea leaves extracts contain various beneficial properties such as antidiabetic, antihypoglycemic and antioxidant activities, which can be enzymatically extracted in water. Vitexin is one of the... more
Mas Cotek or Ficus deltoidea var. deltoidea leaves extracts contain various beneficial properties such as antidiabetic, antihypoglycemic and antioxidant activities, which can be enzymatically extracted in water. Vitexin is one of the antioxidants and remarkable compound of flavone C-glycosides found in Mas Cotek leaves. Cellulase is one of the essential enzymes applied to hydrolyze cellulose into fermentable sugars and therefore, this enzyme was used to extract vitexin in this study. Single parameter approach was used to determine factors influencing the enzymatic-mediated extraction. These factors were enzyme concentration, sample-to-water ratio, temperature, incubation time and agitation. The maximum yield of vitexin found was 0.547% under the optimum conditions: sample-to-water ratio 1:10 g/mL, enzyme concentration 0.4%, incubation time of 240 min, temperature of 50 ± 0.02°C and stirring speed of 200 rpm. The highest amount of reducing sugar obtained was 4.039 mg/mL with the use of 0.4% cellulose. A correlation was also observed between the yield of vitexin and reducing sugars.
- by Aimi Syairah and +1
- •
- Enzyme Technology, Cellulase, Antioxidants, EXTRACTION
Ovarian cancer happens as the result of change in gene and molecular metabolites. Because of chemotherapy side effects in cancer treatment such as drug resistance, using of complementary therapy with herbal and their derivatives is... more
Ovarian cancer happens as the result of change in gene and molecular metabolites. Because of chemotherapy side effects in cancer treatment such as drug resistance, using of complementary therapy with herbal and their derivatives is increased. This study evaluates anti-tumor effects of vitexin on AHRI, p53 and Kras genes expression by Real time PCR. Also metabolite changes due to these variations are measured by 1D NOESY, 1 HNMR. SKOV3 cells treated whit different concentrations of vitexin and determined IC50 by MTT assay. The IC50 was measured as 520µg /ml. Then RNA extracting and building of cDNA done to determine level of genes expression changes. Metabolites extracted by water, chloroformed and methanol and lyophilized samples evaluated by 1HNMR. The expression of AHRI and p53 tumor suppressor genes in the treated cells increased by 1.93 and 1.76 times, respectively, and the expression of Kras oncogene gene decreased by 0.23 times. Maximum changes in metabolites pathways observed in Aminoacyl-tRNA biosynthesis, Biotin, cysteine, methionine, branch amino acids, lysine metabolism, and steroids biosynthesis. Vitexin shows its anti-tumor effects by targeting of several biochemical pathways and reload of metabolites by change in genes which have roll in ovarian cancer. So to confirm this study more evaluations in pathway signaling is needed.