Wt

<p>A, Immunoblots of recombinant His tagged proteins in lysates of RosettaBlue (DE3) cells transformed with EX-Mm01963-B01 plasmid and exposed to 1 mM isopropyl β-Δ-thiogalactoside (IPTG) for different durations at 30 degrees C, B, Immunoblots of soluble His-tagged proteins from RosettaBlue (DE3) cells after purification with Ni-NTA resin. Different protein fractions (F) eluted from Ni-NTA resin by high salt buffer were tested. LF is the lost fraction that was collected just after the lysate was applied onto the resin before elution of the proteins using high salt buffer. Immunoblots are representative of four separate experiments with CS overexpression and purification. C, Citrate synthase activity in the first five fractions of proteins eluted from Ni-NTA resin using high salt buffer for B6 construct and A/J construct of Cs, respectively. Results are representative of four independent experiments. Data are shown as mean ± S.E.</p