This article aims to show the changes occurring during cryopreservation of embryos and plumules of coconut which are responsible of their death or survival. Embryos have been cryopreserved by preculture-dehydration for 24h, 28h, 30h,... more
This article aims to show the changes occurring during cryopreservation of embryos and plumules of coconut
which are responsible of their death or survival. Embryos have been cryopreserved by preculture-dehydration for 24h, 28h, 30h, 34h, 38h and 48h on agar medium containing 600g/L of glucose combined with silica gel. The plumules were cryopreserved by encapsulation dehydration on solid medium containing 0.5 M, 0.75 M and 1M followed by dehydration with 40g of silica gel for different durations before rapid freezing. This study indicates
that the damages undergone by seed samples can be divided into three types. The first stage of changes concerned
the plasmolysis of cells with small vacuoles, condensation of chromatin, changing in the conformation of the DNA
and the nucleus and stopping of mitosis. These types of changes are described in general in the context of a
desiccation tolerance. The second degree of the changes was the retraction of the cytoplasm inside the cell, the
increase in the periplasmic volume. The third degree of modification concerned the deformation of the walls, the
invagination or the lysis of the plasma membrane resulting in the observation of distorted cells and and the
bursting of the nucleus. These two types of modifications are irreversible and correspond to an absence of
regrowth of the samples. Understanding the damage or changes that occur in cryopreserved cells is an important
part of understanding how dehydration and frozen affect the viability of recalcitrant plants cells. These changes
are made by dehydration and accentuated by freezing.
This is the outline: 1. Quelques points de divergence 1. Quelques points de divergence 1.1 L'anthropologie du christianisme 2. Synthèse de cette vision, une théorie générale du réel 2.1 De nouveau, quelques points de divergence 2.2... more
This is the outline: 1. Quelques points de divergence
1. Quelques points de divergence
1.1 L'anthropologie du christianisme
2. Synthèse de cette vision, une théorie générale du réel
2.1 De nouveau, quelques points de divergence
2.2 Limites de la théorie de l'âme chez Tresmontant
3. De la signification philosophique et théologique de la théorie de l'information
3.1 Sur la nature ultime de l'information
4. Tresmontant et la métaphysique du vinculum
5. À propos de la question de la résurrection
6. Conclusion
This article aims to show the changes occurring during cryopreservation of embryos and plumules of coconut which are responsible of their death or survival. Embryos have been cryopreserved by preculture-dehydration for 24h, 28h, 30h, 34h,... more
This article aims to show the changes occurring during cryopreservation of embryos and plumules of coconut which are responsible of their death or survival. Embryos have been cryopreserved by preculture-dehydration for 24h, 28h, 30h, 34h, 38h and 48h on agar medium containing 600g/L of glucose combined with silica gel. The plumules were cryopreserved by encapsulation dehydration on solid medium containing 0.5 M, 0.75 M and 1M followed by dehydration with 40g of silica gel for different durations before rapid freezing. This study indicates that the damages undergone by seed samples can be divided into three types. The first stage of changes concerned the plasmolysis of cells with small vacuoles, condensation of chromatin, changing in the conformation of the DNA and the nucleus and stopping of mitosis. These types of changes are described in general in the context of a desiccation tolerance. The second degree of the changes was the retraction of the cytoplasm inside the cell, the incr...
Abstract: The production of whole plant of Kelussia odoratissima Mozaff. from zygotic embryo culture is an efficient and rapid strategy. Escaping in vitro contamination and long-term seed dormancy, seed embryos can be used for mass... more
Abstract: The production of whole plant of Kelussia odoratissima Mozaff. from zygotic embryo culture is an efficient and rapid strategy. Escaping in vitro contamination and long-term seed dormancy, seed embryos can be used for mass production of this species. After seeds were sterilized, zygotic embryo was excised and cultured on MS, MS1/2 and B5 basal media. Embryos which were grown on B5 efficiently induced root growing and plantlets shape were more natural. By identifying well grown embryos on B5, it was used to determine the most effective nitrogen sources in medium. Media supplemented with 50 mg L-1 casein hydrolysate and 100 mg L-1 arginine were found more proficient to produce whole plant. By applying 50 mg L-1 casein hydrolysate, embryos fresh weight reached its crescendo, whereas 100 mg L-1 arginine induced root growth more efficiently.
