A simple and sensitive method for Cu
2+ detection was developed using the Cu
+-catalyzed alkyne–azide cycloaddition reaction, Fe
3O
4 magnetic nanoparticles (MNPs) as the reaction platform, and a portable blood glucose meter (PGM) as the detection method. Gold nanoparticles
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A simple and sensitive method for Cu
2+ detection was developed using the Cu
+-catalyzed alkyne–azide cycloaddition reaction, Fe
3O
4 magnetic nanoparticles (MNPs) as the reaction platform, and a portable blood glucose meter (PGM) as the detection method. Gold nanoparticles (AuNPs) were labeled with glucose oxidase (GOx) and alkyne-functionalized, terminally thiolated ssDNA (C2). In the presence of Cu
2+ and ascorbate, the functionalized AuNPs were captured onto MNPs modified with azide-functionalized ssDNA (C1) via the Cu
+-catalyzed alkyne–azide cycloaddition reaction. The GOx on the AuNPs’ surface oxidized glucose (Glu) into gluconic acid and H
2O
2, reducing the Glu content in the reaction solution, which was quantitatively detected by the PGM. Under optimal conditions, the PGM response of the system showed a good linear relationship with the logarithm of Cu
2+ concentration in the range of 0.05 to 10.00 μmol/L, with a detection limit of 0.03 μmol/L (3σ). In actual tap water samples, the spiked recovery rate of Cu
2+ was between 92.30% and 113.33%, and the relative standard deviation was between 0.14% and 0.34%, meeting the detection requirements for Cu
2+ in real water samples.
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