The Suppressive Activity of Fucofuroeckol-A Derived from Brown Algal Ecklonia stolonifera Okamura on UVB-Induced Mast Cell Degranulation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structure Elucidation of Phlorotannin
2.2. Effect of F-A on Mast Cell Degranulation Induced by UVB
2.3. Effect of F-A on Intracellular Ca2+ Elevation in UVB-Exposed RBL-2H3 Mast Cells
2.4. Effect of F-A on Cytokine Production in UVB-Exposed RBL-2H3 Mast Cells
2.5. Effect of F-A on ROS Production in UVB-Exposed RBL-2H3 Mast Cells
3. Materials and Methods
3.1. Reagents and Materials
3.2. Cell Culture and Cell Viability Assay
3.3. Histamine Release Assay
3.4. Measurement of the Intracellular Ca2+ Level
3.5. Measurement of Cytokine Production
3.6. Measurement of ROS Production
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Vo, T.S.; Kim, S.-K.; Ryu, B.; Ngo, D.H.; Yoon, N.-Y.; Bach, L.G.; Hang, N.T.N.; Ngo, D.N. The Suppressive Activity of Fucofuroeckol-A Derived from Brown Algal Ecklonia stolonifera Okamura on UVB-Induced Mast Cell Degranulation. Mar. Drugs 2018, 16, 1. https://doi.org/10.3390/md16010001
Vo TS, Kim S-K, Ryu B, Ngo DH, Yoon N-Y, Bach LG, Hang NTN, Ngo DN. The Suppressive Activity of Fucofuroeckol-A Derived from Brown Algal Ecklonia stolonifera Okamura on UVB-Induced Mast Cell Degranulation. Marine Drugs. 2018; 16(1):1. https://doi.org/10.3390/md16010001
Chicago/Turabian StyleVo, Thanh Sang, Se-Kwon Kim, BoMi Ryu, Dai Hung Ngo, Na-Young Yoon, Long Giang Bach, Nguyen Thi Nhat Hang, and Dai Nghiep Ngo. 2018. "The Suppressive Activity of Fucofuroeckol-A Derived from Brown Algal Ecklonia stolonifera Okamura on UVB-Induced Mast Cell Degranulation" Marine Drugs 16, no. 1: 1. https://doi.org/10.3390/md16010001