Search Results (624)

The effect of different treatments on the seed quality of crimson clover (Trifolium incarnatum L.) from six localities in eastern Serbia was investigated. The aim of this study was to improve seed quality and seedling growth of T. incarnatum using eco-friendly treatments. Tests were carried out under laboratory and field conditions, using hot water and air-drying temperatures. Seed quality parameters included germinated seeds, dormant seeds, dead seeds, seedling growth, and abnormal seedlings, all expressed as percentages. The water absorption test confirmed the presence of physical seed dormancy in crimson clover. The best results were achieved with a 30-min hot water treatment, which increased water absorption and reduced the percentage of dormant seeds. Longer exposure times increased the percentage of dead seeds and abnormal seedlings, whereas shorter exposure times increased the percentage of dormant seeds and reduced germination. There was strong agreement between the germination results obtained under laboratory and field conditions. These findings illustrate new biotechnological approaches to enhancing seed quality. Full article

The use of water-efficient soil amendments has gained increasing importance in agriculture, particularly in regions facing water scarcity. So, this study evaluates the impact of silica and nano-silica hydrogels on soil water retention, crop yield, and crop water productivity under variable irrigation regimes. Using a randomized complete block design with furrow irrigation, the experiment tested different hydrogel application rates and irrigation levels in rice (Oryza sativa L.) and clover (Trifolium alexandrinum L.) across two growing seasons. Statistical tests, including ANOVA and t-tests, confirm that nano-silica hydrogel significantly improves soil properties, yield, and crop water productivity (CWP), especially at moderate irrigation levels (70–90% of water requirements). In the first season, nano-silica hydrogel enhanced rice yield, with a maximum yield of 10.76 tons ha⁻¹ with 90% irrigation and 119 kg ha⁻¹ of hydrogel compared with other treatments. In the second season, clover yields were also positively affected, with the highest fresh forage yield of 5.02 tons ha⁻¹ with 90% irrigation and 119 kg ha⁻¹ nano-silica hydrogel. Despite seasonal variation, nano-silica hydrogel consistently outperformed silica hydrogel in terms of improving soil water retention, reducing bulk density, and enhancing hydraulic conductivity across different irrigation levels. Principal Component Analysis (PCA) revealed that nano-silica hydrogel significantly improved soil water retention properties, including the water-holding capacity (WHC), field capacity (FC), and available water (AW), and reduced the wilting point (WP). These improvements, in turn, led to increased crop yield and water productivity, particularly at moderate irrigation levels (70–90% of the crop’s total water requirements. These findings highlight the potential of nano-silica hydrogel as an effective amendment for improving soil water retention, enhancing crop productivity, and increasing crop water productivity under reduced irrigation conditions. Full article

Mixed legume/grass grasslands are the most significant type of artificial grassland in rain-fed semi-arid regions. Understanding the contributions of legumes and grasses to grassland productivity, as well as the nitrogen-sharing mechanisms between them, is crucial to maintaining the sustainability, stability, and high yield of mixed grasslands. In this study, four commonly used cultivated species were selected: smooth bromegrass (Bromus inermis Leyss.), orchardgrass (Dactylis glomerata L.), sainfoin (Onobrychis viciifolia Scop.), and red clover (Trifolium pratense L.). Combinations of two and three species of legumes and grasses were established, with monoculture serving as the control. The results revealed that in all the monocultures and mixed grasslands comprising two or three species, the average dry matter yield (DMY) of mowed grasslands in 2017 was significantly higher than in 2018, while the average DMY of grazed summer regrowth in 2018 surpassed that of 2016 and 2017. Over the period from 2016 to 2018, smooth bromegrass and sainfoin gradually dominated the mixed grasslands, while orchardgrass and red clover exhibited a declining abundance. Over time, the ratio and amount of nitrogen (N) fixation in legumes significantly increased in both the monoculture and mixed grasslands. Similarly, the amount of nitrogen (N) received by grasses also increased significantly in mixed grasslands. However, the proportion of nitrogen fixed by legumes remained below 10% in 2016, 20% in 2017, and 30% in 2018. In contrast, nitrogen transfer from legumes to smooth bromegrass was less than 10%, while in orchardgrass, it was even lower, at less than 2%. The interannual variability in dry matter yield (DMY) and nitrogen contribution in the mixed grasslands of rain-fed semi-arid areas is primarily influenced by forage adaptability and average annual precipitation. Increasing the proportion of grazed forage relative to hay in annual forage consumption should be considered, as more extensive grazing can reduce damage from field rodents and provide higher forage quality at lower costs and energy consumption. To maintain grassland productivity, targeted grazing should be carefully planned and implemented. Full article

The hybrid progeny (1-1) resulting from the cross between Caucasian clover and white clover initially demonstrated an inability to fix nitrogen naturally via spontaneous nodulation. However, following inoculation with specific rhizobia strains derived from the Trifolium genus, successful nodulation and nitrogen fixation were observed in the 1-1 progeny, resulting in enhanced biomass production and adaptability. To explore in greater depth the mechanisms driving nitrogen fixation in these hybrid progeny, the inoculation was carried out using the dominant rhizobia strain (No. 5), isolated from Mengnong Clover No. 1. Root samples were collected at 3, 6, and 9 days post inoculation for RNA sequencing. A total of 1755 differentially expressed unigenes were identified between the control and treatment groups. KEGG pathway analysis highlighted key pathways associated with nodule nitrogen fixation. In combination with Weighted Gene Co-expression Network Analysis (WGCNA) and Gene Set Enrichment Analysis (GSEA), several differentially expressed genes were identified, suggesting their potential contribution to nitrogen fixation. Noteworthy among these, the gene TRINITY_DN7551_c0_g1 in the Phenylpropanoid biosynthesis pathway (MAP00940) emerged as a key candidate. This study offers valuable RNA-seq data, contributing significantly to the understanding of the molecular regulatory mechanisms underpinning nodule nitrogen fixation in legumes, thereby laying a solid foundation for future investigations into the hybrid progeny of Caucasian and white clover crosses. Full article

A field experiment was conducted in two seasons (2019–2020 and 2020–2021) at three locations in Slovenia (Rogoza, Fala, and Brežice) to evaluate the yield and silage quality of winter cover crops (WCCs). The experiment included Italian ryegrass (IR) in pure stands, fertilized with nitrogen in spring, and mixtures of crimson clover (CRC), red clover (RC), and IR+CRC+RC without nitrogen fertilization in spring. The highest dry matter yield (DMY) was observed in IR+CRC+RC (4.98 t ha⁻¹). For fresh feed, the CRC+RC treatment had significantly higher (p < 0.05) crude protein (208 g kg⁻¹ DM), nitrate nitrogen (116.7 mg kg⁻¹ DM), and buffering capacity (1290 mmol kg⁻¹ DM) but significantly lower (p < 0.05) dry matter (128 g kg⁻¹) and water-soluble carbohydrates (121 g kg⁻¹ DM). For silage, the CRC+RC treatment had significantly lower (p < 0.05) dry matter (476 g kg⁻¹ silage), metabolic energy (9.65 MJ kg⁻¹ DM), net energy of lactation (5.77 MJ kg⁻¹ DM), and neutral detergent fiber (375 g kg⁻¹ DM) but higher ammonia nitrogen (66.5 g kg⁻¹ of total nitrogen), crude protein (158 g kg⁻¹ DM), and acid detergent fiber (279 g kg⁻¹ DM). No significant differences (p > 0.05) were found among treatments for acetic, lactic, and butyric acid, crude fat, pH, and soil mineral nitrogen (N_min). The results of the study show that the same or higher DMY and a comparable quality of highly wilted silage can be produced with mixed Italian ryegrass and clovers compared with those of Italian ryegrass in pure stands. The experiment aimed to determine whether clover-based mixtures can achieve comparable silage quality and dry matter yield without spring N fertilizers compared with those of pure stands of Italian ryegrass fertilized in spring. Full article

White clover (Trifolium repens L.) is cultivated worldwide as a forage crop, green manure, and turfgrass, valued for its adaptability and broad distribution. Although numerous studies have investigated the adverse effects of drought stress on white clover growth and yield, a comprehensive bibliometric review has been lacking. To address this gap, we analyzed relevant publications from the Web of Science Core Collection (1990–2024) using VOSviewer (1.6.19.0) and R (4.3.1) software. Our findings reveal a consistent annual increase in research outputs, indicating sustained scholarly efforts to enhance white clover’s drought tolerance. China, New Zealand, Australia, the United States, and France lead in publication volume and maintain active international collaborations. Keyword co-occurrence analysis underscores the importance of phenotypic, physiological, and molecular mechanisms linked to drought resistance, particularly regarding plant growth and yield. Emerging directions include further exploration of transgenic technologies and molecular pathways to bolster white clover’s resilience under water-limited conditions. Overall, these insights offer a theoretical foundation for future research and provide a valuable reference for advancing sustainable agricultural practices in arid and semi-arid environments. Full article

Groundwater pollution negatively impacts aquatic ecosystems and human health. On the other hand, conservation practices can help reduce groundwater and surface water pollution. Baseflow from agricultural fields can be an important source of nitrate-nitrite (NN) loads in lakes and other surface water bodies. Riparian agroforestry buffers can be an effective barrier between groundwater NN and surface water bodies. The study aimed to determine the effects of agroforestry buffers and widths on groundwater nitrate-nitrite (NN) exports from an agricultural grazing area into a farm lake using flow and solute transport models. The flow and solute models were calibrated and validated for the weather and land use (grazing) conditions observed during the monitoring period, and these conditions were repeated throughout the 10-year projection. The calibration and validation of the flow and solute transport models were satisfactory, yielding determination coefficients R² > 0.95 and Nash-Sutcliffe coefficients > 0.94. The area of study was modeled under four scenarios: tree-only buffers [cottonwood (Populus deltoides Bortr. ex Marsh.)]; grass-only buffers ([Tall fescue Schedonorus phoenix (Scop.) Holub, Red clover (Trifolium pretense L.), and Lespedeza (Lespedeza Michx)]); tree + grass buffers (a combination of the same tree and grass species of the other two scenarios; and a no-buffer scenario. The tree-only, grass-only, and tree + grass buffers reduced the total mass of NN discharged from the study unit to the lake by 98%, 97%, and 99%, respectively, compared to the no-buffer scenario. Doubling the buffer width from 15 m to 30 m decreased the NN discharge to the lake by 16-fold. Moreover, 7.5 m wide buffers had up to nine times greater NN discharge than 15 m buffers. Results show that agroforestry buffers with trees and grasses in riparian areas significantly remove NN exports in groundwater from agricultural fields, protecting the environment and human health. Full article

The aim of this experiment was to investigate the differences in the uptake and accumulation of rare earth elements (REEs) between selected plant species and the substrates used (soil with increased REE content, ash, and smelter waste). Eight plant species were included in the study: common yarrow (Achillea millefolium), false mayweed (Triplerosperum maritimum), tall fescue (Festuca arundinacea), marigold (Tagetes sp.), maize (Zea mays), white mustard (Sinapis alba), red clover (Trifolium pratense L.), and autumn fern (Dryopteris erythrosora). The study focused on the following REE representatives: lanthanum (La), cerium (Ce), europium (Eu), and gadolinium (Gd). Plant samples, divided into roots and shoots, were analyzed by ICP-MS. The obtained REE concentrations in plant tissues ranged from 9 to 697 µg kg⁻¹ (La), 10 to 1518 µg kg⁻¹ (Ce), 9 to 69 µg kg⁻¹ (Eu), and 9 to 189 µg kg⁻¹ (Gd). To determine the ability of plants to phytoextract REE, two factors were calculated: the translocation factor (TF) and the bioconcentration factor (BCF). The highest TF value was obtained for D. erythrosora growing on a substrate consisting of soil with increased REE content (Gd, TF = 4.03). Additionally, TF > 1 was obtained for all REEs in T. pratense L. In the experiment, the BCF was lower than 1 for all the plants tested. The study provided insight into the varying ability of plants to accumulate REEs, depending on both the plant species and the chemical properties of the substrate. Full article

It is generally recognized that clovers represent a major nutrient used in ruminants’ diets due to their composition, which is high in protein content and low in fiber content. Investigating the nutritional quality of red and white clover genotypes, and classifying the genetic materials according to their primary quality attributes were the main goals of the current study. During a two-year experiment, we assessed their performance stability. Twelve red clover (Trifolium pratense L.) and twelve white clover (Trifolium repens L.) genetic materials were cultivated in a randomized complete block experimental design. Crude protein (CP%), acid detergent fiber (ADF%) and neutral detergent fiber (NDF%) concentrations were measured, and the relative feed value (RFV) was calculated using the estimates of digestible dry matter (DDM%) and potential dry matter intake (DMI% of body weight) of the forage. The average CP% content for white clover varied between 17.18% (REP-4) and 20.55% (REP-9) during the two years of testing. Regarding the red clover, the populations PRA-4, PRA-3, PRA-5, and PRA-10 exhibited the highest CP% content (19.98%) and the lowest ADF% (less than 29%). Although the variation was stable across the years, the profile of this variation of CP, ADF, and NDF for each clover species differs. The CP% was related negatively to ADF% and thus positively to DDM in both species but not with the same allocation, forming different clusters. PRA-3 and PRA-4 for the red clover and REP-5 for the white clover genetic materials exhibited characteristics belonging to the other species studied regarding their content in protein and fiber. Because of their relationship, CP and DDM could be improved simultaneously by breeders. This study revealed that ROZETA and PRA-4 were more stable genetic materials in red clover, and GRASSLANDS HUIA, RIVENDEL, and REP-5 in white clover. Full article

Conservation Agriculture practices in Organic Farming can enhance the sustainability of these farming systems. However, these practices have economic and environmental implications for farmers, which must be considered. In the present study, eight technical itineraries were compared in tomato cultivation. These differed in how reduced and no-tillage practices were used to manage four soil cover types and to control weeds. The itinerary’s gross salable production (GSP), gross income (GI), and CO₂ emissions were evaluated. In the second growing season, the no-tillage itinerary values of both GSP and GI were lower than those based on reduced tillage (34,681.03 and 71,891.58 EUR ha⁻¹, respectively). The use of cover crops tendentially resulted in an increase in GSP in both growing seasons compared to cultivation on bare soil (8190.00 and 41,959.89 EUR ha⁻¹ in 2020 and 2021, respectively), particularly with clover monoculture and a clover–rye mix in 2020 (25,326.60 and 25,818.97 EUR ha⁻¹, respectively) and with clover monoculture in 2021 (69,310.18 EUR ha⁻¹). A similar trend was also observed for GI. Cover crop adoption was related to a higher CO₂ emissions (642.73 and 234.84 kg ha⁻¹ in 2020 and 353.23 and 213.30 kg ha⁻¹ in 2021, for itineraries based on reduced-tillage and no-tillage, respectively). Further studies could focus on the economic and environmental evaluation of these systems in the same pedoclimatic conditions but over the long term, quantifying the various environmental benefits of cover crops. Full article

A two-year field trial (2021–2023) was conducted to evaluate the impacts of cover crop (CC) inclusion (cereal rye, crimson clover, mixtures of cereal rye and crimson clover, and fallow control) and nitrogen (N) fertilization (0, 22, 45, 90, 135, and 180 kg N ha⁻¹) in cotton production in sandy soils. Cover crops were planted in October and terminated two weeks before cotton planting in May. The N was applied in split applications. Cover crop aboveground biomass was collected, oven dried, and weighed, and then used for C and N analyses. Soils were sampled at CC termination and analyzed for biogeochemical properties. Cotton lint yields and agronomic nutrient use efficiency (aNUE) were estimated. The CC mixtures provided higher organic C and N inputs as residue returns than individual species. Integrating CCs had limited impacts on measured soil properties. Integrating CCs resulted in positive, neutral, and adverse effects on lint yield and aNUE depending on species and growing seasons. Applying N at 22 kg ha⁻¹ increased lint yields in 2022, while higher rates did not improve the yields further. Similar patterns of impacts were observed at the N rate of 45 kg ha⁻¹ in 2023. The results indicated that integrating CC mixtures can favor long-term C and N sequestration in sandy soils. However, optimal management is essential to realize their benefits. Relevant research to better understand the decomposition of their residues would be beneficial in improving the management of desirable outcomes. Full article

Idiopathic pulmonary fibrosis (IPF) has been associated with mitochondrial dysfunction. We investigated whether mitochondrial DNA variants in peripheral blood leukocytes (PBLs), which affect proteins of the respiratory chain and mitochondrial function, could be associated with an increased risk and poor prognosis of IPF. From 2020 to 2022, we recruited 36 patients (age: 75.3 ± 8.5; female: 19%) with IPF, and 80 control subjects (age: 72.3 ± 9.0; female: 27%). The mitochondrial genome of peripheral blood leukocytes was determined using next-generation sequencing. During a 45-month follow-up, 10 (28%) patients with IPF remained stable and the other 26 (72%) progressed, with 12 (33%) mortalities. IPF patients had more non-synonymous (NS) variants (substitution/deletion/insertion) in mitochondrial COX3 gene (coding for subunit 3 of complex IV of the respiratory chain), and more mitochondrial tRNA variants located in the anticodon (AC) stem, AC loop, variable loop, T-arm, and T-loop of the tRNA clover-leaf structure in PBLs than the control group. The succumbed IPF patients were older, had lower initial diffusion capacity, and higher initial fibrosis score on high-resolution computerized tomography (HRCT) than the alive group. NS variants in mitochondrial COX3 gene and tRNA variants in PBLs were associated with shorter survival. Our study shows that (1) leukocyte mitochondrial COX3 NS variants are associated with risk and prognosis of IPF; (2) leukocyte mitochondrial tRNA variants located in the AC stem, AC loop, variable loop, T-arm, and T-loop of the tRNA clover-leaf structure are associated with risk, and the presence of tRNA variants is associated with poor prognosis of IPF. Full article

Energy consumption in buildings is a major contributor to greenhouse gas emissions, primarily due to the extensive burning of fossil fuels. This study focuses on an innovatively designed building named The Clover and utilises IES-VE software (2024) to create a digital twin for the building’s performance prediction. The goal is to achieve a zero-carbon-emission building through energy-efficient strategies, including the use of air-source heat pumps and renewable energy systems for sustainable heating, cooling, and electricity. Dynamic simulations conducted with the software analyse key performance metrics, including annual heating and cooling demands, electricity consumption, carbon emissions, and renewable energy supply. The results indicate that a 53% reduction in CO₂ emission is achieved when a heat pump system is applied instead of boiler and chiller systems. A total of 1243.96 MWh and 41.18 MWh of electricity can be generated by PV panels and wind energy systems. The net annual electricity generation from the energy system of the building is 191.64 MWh. Therefore, the results demonstrate that the building’s energy needs can be successfully met through on-site electricity generation using advanced perovskite–silicon tandem solar PV panels and wind turbines. This case study provides valuable insights for architects and building services engineers, offering a practical framework for designing green, energy-efficient, zero-carbon buildings and advancing the path to net zero. Full article

Background/Objectives: The most common type of breast cancer (BRC) in women is estrogen/progesterone receptor positive. First-line treatment includes endocrine therapy, either with aromatase inhibitors or tamoxifen to reduce estrogen levels. Among the side effects produced by this treatment, aromatase inhibitor-induced arthralgia is the most common, affecting the patients’ overall health and quality of life (QoL). The objectives here were to evaluate interventions examining the impact of modified diets, supplements, and/or some food components on health outcomes in BRC patients undergoing endocrine therapy. Methods: The literature search was performed in PubMed, Scopus, and Web of Science from June 2024, as well as manually, through the end of November 2024. The search was limited to studies of women diagnosed with estrogen/progesterone-receptor-positive BRC with selected articles reporting interventions with diet, food, or supplement intake and examining the relevant health outcomes. Studies not focusing on BRC patients undergoing endocrine therapy or not including specific health outcomes were excluded. Results: The search uncovered 1028 studies; after the removal of duplicates, abstracts, and irrelevant studies, 53 were closely examined, with 26 evaluated and presented here. The outcomes were changes in bone and body composition, cardiovascular disease risks, inflammation, and QoL. Conclusions: The examined evidence suggests that adherence to dietary patterns such as the Mediterranean or a low-fat diet, and a higher intake of fruits and vegetables were beneficial for various outcomes. Additionally, supplementation with some foods/components (dried plum, red clover) contributed to improving/maintaining bone and body composition, especially in overweight/obese patients. Supplementation with vitamin D or omega-3 improved lipid and angiogenic parameters and QoL. Although these results are promising, the effects of each supplement/food cannot be summarized due to the diverse nature of study designs, patients, and supplement dosages. Further studies are needed to explore the effects of specific nutritional interventions (including the newest, like fasting-mimicking diets and whole-grain cereal diets) on various health outcomes in BRC survivors during endocrine therapy, and to derive universal recommendations. Full article

Caucasian clover (Trifolium ambiguum M.Bieb.) is a perennial legume known for its exceptional cold tolerance, commonly used in agriculture and ecosystems in cold climates. Given the impact of climate change, enhancing the cold adaptation of Caucasian clover is crucial for sustaining agricultural productivity. This study employs metabolomics, transcriptomics, and Weighted Gene Co-expression Network Analysis (WGCNA) to investigate the molecular mechanisms of Caucasian clover’s response to low-temperature stress. Metabolomic analysis showed that low-temperature stress triggered the accumulation of fatty acids, amino acids, and antioxidants, which are critical for maintaining membrane stability and antioxidant capacity, thus protecting the plant from oxidative damage. Transcriptomic analysis revealed significant upregulation of genes involved in cold adaptation, particularly those related to antioxidant defense, membrane lipid repair, and signal transduction, including genes in the ABA signaling pathway and antioxidant enzymes, thereby improving cold tolerance. WGCNA identified gene modules closely linked to cold adaptation, especially those involved in antioxidant defense, fatty acid metabolism, signal transduction, and membrane repair. These modules function synergistically, with coordinated gene expression enhancing cold resistance. This study also investigated the isoflavonoid biosynthesis pathway under low-temperature stress, highlighting its role in enhancing antioxidant capacity and cold tolerance. Low-temperature stress induced upregulation of key enzyme genes, such as Isoflavone Synthase (IFS) and Isoflavone-7-O-Glucosyltransferase (IF7GT), promoting antioxidant metabolite accumulation and further enhancing the plant’s cold adaptation. Overall, this study offers novel molecular insights into the cold tolerance mechanisms of Caucasian clover and provides valuable theoretical support for breeding cold-resistant crops in cold climates. Full article