Search Results (2,966)

The premium connection is an important section of the tubing column. Under intricate downhole conditions, axial vibration generates alternating loads that cause energy dissipation between the sealing surfaces of the premium connection, reducing sealing performance. To investigate this issue, the mutual conversion process of sticking, slipping, and macroscopic slipping stages between the sealing surfaces of the premium connection under axial loads must be assessed. In this study, a finite element analysis model of a taper–taper Φ88.9 mm × 6.45 mm P110 premium connection is developed based on the discrete Iwan model’s ontological relationship, and the sealing surface’s force–displacement hysteresis curve is obtained. The equivalent Iwan model for this particular premium connection is constructed by discretizing the hysteresis curve and identifying the model’s four sets of parameters. The correctness of the parameter identification method of the equivalent Iwan model is verified by comparing and analyzing the similarity of the two models. The energy dissipation in the sealing surfaces of the premium connection for different working conditions under dynamic loading is analyzed. This study reveals that the area similarity of the hysteresis curves of the two models is more than 92%, while the positional error is less than 2%. The sealing surface displacement amplitude of the premium connection is between 0.04 mm and 0.07 mm, while the sealing surface energy dissipation increases linearly, which may lead to a decline in sealing performance. Full article

Hypoxia is one of the factors severely affect renal function, and, in severe cases, it can lead to renal fibrosis. Although much progress has been made in identifying the molecular mediators of fibrosis, the mechanisms that govern renal fibrosis remain unclear, and there have been no effective therapeutic anti-fibrotic strategies to date. Mammals exposed to low oxygen in the plateau environment for a long time are prone to high-altitude disease, while yaks have been living in the plateau for generations do not develop kidney fibrosis caused by low oxygen. It has been suggested that metabolic reprogramming occurs in renal fibrosis and that pyruvate dehydrogenase kinase 1 (PDK1) plays a crucial role in metabolic reprogramming as an important node between glycolysis and the tricarboxylic acid cycle. The aim of this study was to investigate the effects of hypoxia on the renal tissues and renal interstitial fibroblasts of yaks. We found that, at the tissue level, HIF-1α, PDK1, TGF-β1, Smad2, Smad3, and α-SMA were mainly distributed and expressed in tubular epithelial cells but were barely present in the renal mesenchymal fibroblasts of healthy cattle and yak kidneys. Anoptical density analysis showed that in healthy cattle kidneys, TGF-β1, Smad2, and Smad3 expression was significantly higher than in yak kidneys (p < 0.05), and HIF-1α and PDK1 expression was significantly lower than in yak kidneys (p < 0.05). The results at the protein and gene levels showed the same trend. At the cellular level, prolonged hypoxia significantly elevated PDK1 expression in the renal mesangial fibroblasts of cattle and yak kidneys compared with normoxia (p < 0.05) and was proportional to the degree of cellular fibrosis. However, PDK1 expression remained stable in yaks compared with renal interstitial fibroblast-like cells in cattle during the same hypoxic time period. At the same time, prolonged hypoxia also promoted changes in cellular phenotype, promoting the proliferation, activation, glucose consumption, lactate production, and anti-apoptosis in the both of cattle and yaks renal interstitial fibroblasts The differences in kidney structure and expression of PDK1 and HIF-1α in kidney tissue and renal interstitial fibroblasts induced by different oxygen concentrations suggest that there may be a regulatory relationship between yak kidney adaptation and hypoxic environment at high altitude. This provides strong support for the elucidation of the regulatory relationship between PDK1 and HIF-1α, as well as a new direction for the treatment or delay of hypoxic renal fibrosis; additionally, these findings provide a basis for further analysis of the molecular mechanism of hypoxia adaptation-related factors and the adaptation of yaks to plateau hypoxia. Full article

In order to study the corrosion resistance of 904L composite plate pressure vessels under a high-temperature and high-pressure gas field environment, the pitting corrosion and stress corrosion cracking resistance of a 904L composite plate body and weld material were compared with those of a 2205 composite plate and 825 composite plate, which are used in high-temperature and high-pressure gas field environments. The results showed that the pitting resistance of the 904L composite plate was lower than that of the 825 composite plate and higher than that of a 2205 solid-solution pure material plate and a 2205 composite plate. The corrosion resistance of the 625 welding material is higher than that of the E385 welding material. In the simulation of the corrosion environment of a high-temperature and high-pressure gas field, the corrosion rates of the 904L composite plate body, welding seam, and surfacing welding were all less than 0.025 mm/a, indicating slight corrosion, and the sensitivity coefficient of chloride stress corrosion cracking was less than 25%, indicating low sensitivity. The 904L composite plate met the requirements of corrosion resistance for pressure vessel materials in a high-temperature and high-pressure gas field environment. Full article

Hydrogen doping using existing natural gas pipelines is a promising solution for hydrogen transportation. A large number of non-metallic seals are currently used in long-distance natural gas pipelines. Compared with metallic seals, non-metallic seals have the advantages of corrosion resistance, light weight, and easy processing, which can improve the safety and economy of pipelines. In order to ensure the long-term safe use of seals in hydrogen-doped natural gas pipelines, this paper selects the non-metallic seals commonly used in long-distance natural gas pipelines and carries out the hydrogen-doped sealing test, hydrogen-doped aging test, and hydrogen-doped anti-explosion test on the non-metallic seals under the conditions of different hydrogen-doped ratios. At the same time, combined with the actual working conditions of a hydrogen-doped natural gas pipeline, the external environment, and other factors, the applicability evaluation index system was established, and the applicability evaluation model based on hydrogen-doped physical and chemical properties, fuzzy comprehensive evaluation, and the structural entropy weight method was developed and applied in the field. The results show that the evaluation result of nitrile rubber in soft seals is 1.7845, and the evaluation result of graphite-polytetrafluoroethylene material in hard seals is 1.5988, and both of them are at a good level. This paper provides technical support and judging strategies for the selection of non-metallic sealing materials for hydrogen-doped natural gas pipelines. Full article

Exposure to even low levels of the environmental pollutant cadmium (Cd) increases the risk of kidney damage and malfunction. The body burden of Cd at which these outcomes occur is not, however, reliably defined. Here, multiple-regression and mediation analyses were applied to data from 737 non-diabetic Thai nationals, of which 9.1% had an estimated glomerular filtration rate (eGFR) ≤ 60 mL/min/1.73 m² (a low eGFR). The excretion of Cd (E_Cd), and renal-effect biomarkers, namely β₂-microglobulin (E_β2M), albumin (E_alb), and N-acetylglucosaminidase (E_NAG), were normalized to creatinine clearance (C_cr) as E_Cd/C_cr E_β2M/C_cr, E_alb/C_cr, and E_NAG/C_cr. After adjustment for potential confounders, the risks of having a low eGFR and albuminuria rose twofold per doubling E_Cd/C_cr rates and they both varied directly with the severity of β₂-microglobulinuria. Doubling E_Cd/C_cr rates also increased the risk of having a severe tubular injury, evident from E_NAG/C_cr increments [POR = 4.80, p = 0.015]. E_NAG/C_cr was strongly associated with E_Cd/C_cr in both men (β = 0.447) and women (β = 0.394), while showing a moderate inverse association with eGFR only in women (β = −0.178). A moderate association of E_NAG/C_cr and E_Cd/C_cr was found in the low- (β = 0.287), and the high-Cd body burden groups (β = 0.145), but E_NAG/C_cr was inversely associated with eGFR only in the high-Cd body burden group (β = −0.223). These discrepancies together with mediation analysis suggest that Cd-induced nephron destruction, which reduces GFR and the tubular release of NAG by Cd, involves different mechanisms and kinetics. Full article

CO₂ injection composite fracturing is an effective method for shale oil and gas well development. The downhole casing is prone to uniform corrosion, pitting, perforation, and even corrosion fracture in the CO₂ environment. Therefore, it is particularly important to reveal the physical characteristics of CO₂ under actual geological conditions and the impact of CO₂ corrosion on the performance of casing. A mathematical model for the temperature and pressure field of CO₂ in the wellbore under fracturing conditions is established in this paper, and the temperature and pressure distribution along the depth of the well is calculated. By optimizing the CO₂ state equation and using the S-W equation, Lee model, and RK model to calculate the CO₂ density, viscosity and compression factor, respectively, the phase distribution pattern of CO₂ along the actual wellbore is obtained. Through CO₂ corrosion tests on the casing, the influence of temperature and CO₂ concentration on the corrosion rate of the casing is clarified. The peak corrosion rate of Q125 steel corresponds to 80 °C, and the corrosion rate increases with the increase in CO₂ concentration. Finally, a prediction model for the uniform corrosion rate of casing under different temperatures and CO₂ concentration conditions is obtained, which can provide technical support for the design of CO₂-enhanced fracturing technology. Full article

As is widely known, the construction industry is one of the sectors with a large contribution to global carbon emissions. Despite numerous efforts in the construction industry to develop low-carbon materials, there is a limited number of studies quantifying and presenting the overall environmental impact when these materials are applied in a construction project as structural members. To address this gap, this study focuses on assessing the life-cycle performance of novel structural aluminium alloy–concrete composite columns. In this paper, the environmental impacts and economic aspects of a concrete-filled aluminium alloy tubular (CFAT) column and a concrete-filled double-skin aluminium alloy tubular (CFDSAT) column were assessed using life-cycle assessment (LCA) and life-cycle cost analysis (LCCA) approaches, respectively. The cradle-to-grave system boundary is considered for these analyses to cover the entire life-cycle. A concrete-filled steel tubular (CFST) column is also assessed for reference. All columns are designed to have the same load-carrying capacity and, thus, are compared on a level-playing basis. A comparison is also made of the self-weight of these columns. In particular, the self-weight of the CFST column is reduced by around 17% when the steel tube is replaced by an aluminium alloy tube, and decreased by 47% when the double-skin technique is adopted in CFDSAT columns. The LCA results indicate that the CO₂ emission of CFST and CFAT is almost the same, which is 21% less than the CFDSAT columns due to the use of high aluminium in the latter. The LCCA results show that the total life-cycle cost of CFAT and CFDSAT columns is around 29% and 14% lower, respectively, than that of the CFST column. Finally, a sensitivity analysis was carried out to evaluate the effects of data and assumptions on the life-cycle performance of the examined columns. Full article

A tapered concrete-filled double-skin steel tubular (TCFDST) structure has been used as the main framework in transmission towers, offshore facility platforms, and turbine towers owing to its excellent mechanical properties. In order to solve the difficulties of calculating the axial compressive capacity of TCFDST members due to the variations in cross-section, this paper applied heuristic optimization algorithms such as Genetic Algorithms (GAs), Particle Swarm Optimization (PSO), Simulated Annealing (SA), and Ant Colony Optimization (ACO) to enhance a Backpropagation Neural Network (BPNN) model. A predictive model incorporating both global and local optimization strategies for the axial compressive capacity of a TCFDST structure is proposed. A comprehensive axial database for TCFDST members, comprising 1327 sets of experimental and finite element analysis results, was established, with ten types of component dimensions and material parameters selected as input variables and compressive bearing capacity as the output variable. This study developed and assessed four BPNN models, each optimized by a different heuristic algorithm, against various machine learning algorithms and standards. The heuristic-algorithm-optimized BPNN models demonstrated superior accuracy in predicting the axial compressive capacity of TCFDST members. Through parametric analysis, this study identified the relationship between the model’s bearing capacity predictions and each input parameter, confirming the model’s broad applicability. The optimized BPNN model, refined with heuristic algorithms, provides a significant reference for addressing the computational challenges associated with the load-bearing capacity of TCFDST structures and facilitating their application. Full article

Antioxidants are essential for mitigating oxidative stress and maintaining vascular health. Endothelial colony-forming cells (ECFCs) are pivotal in endothelial regeneration and angiogenesis and serve as a model to study the diversity of endothelial cells across various organs. This study evaluated the effects of peel, pulp, and seed extracts from Diospyros digyna Jacq. fruit (black persimmon) on human cord blood-derived ECFCs (CB-ECFCs) to determine how the distinct antioxidant profiles of the fruit’s different parts influence cellular functions. The extracts did not affect endothelial marker expression, cell proliferation, or nitric oxide production, indicating no cytotoxic or inflammatory effects. However, functional assays revealed that the seed extract significantly enhanced tube formation, increasing closed tubular networks by 1.5-fold. All extracts promoted cell migration, with the seed extract demonstrating the most substantial effect, surpassing even vascular endothelial growth factor (VEGF). Additionally, the seed extract exhibited the strongest reduction in cellular senescence, both before and after oxidative stress induction with H₂O₂. These findings underscore the potential of black persimmon extracts, especially from the seed, to enhance the regenerative capabilities of CB-ECFCs and reduce cellular senescence without affecting the normal endothelial phenotype. This positions them as promising candidates for developing endothelial cell therapies and advancing vascular regeneration. Full article

The in-plane loading conditions of carbon fiber/epoxy composite (CFRP) and aluminum nested-tube-reinforced expanded polypropylene (EPP) blocks were empirically examined. This study used crashworthiness metrics to estimate the best design configuration under quasi-static loading rates. The experimental phase began with lateral loading testing of single and nested aluminum and CFRP specimen. In-plane crushing experiments were performed on EPP foam blocks reinforced with nested tubes. Both single and nested aluminum tubes had comparable force–response curves and maintained their load-bearing capacity throughout testing. Despite a load-carrying capacity drop above a particular displacement threshold, the CFRP specimens had superior specific energy absorption (SEA) values due to their lightweight nature. The triple-tube nested specimens with two smaller tubes exhibited the best SEA results (1.72 and 1.88 J/g, respectively, for the aluminum and CFRP nested samples). During concurrent tube deformation, the nested samples showed a synergistic connection that increased energy absorption, especially in the EPP foam blocks with reinforced tubes. The study also examined the effects of building nested specimens with aluminum exterior tubes and CFRP inner tubes, and vice versa. This method showed that CFRP tubes within aluminum outer tubes lowered specimen weight (from 93.1 g to 67.7 g) and energy absorption (from 160.2 J to 153.3 J). However, the weight reduction outweighed the energy absorption, increasing SEA values for certain composite material configurations (from 1.72 J/g to 2.26 J/g). Full article

DNA-binding protein A (DbpA) belongs to the Y-box family of cold shock domain (CSD) proteins that bind RNA/DNA and exert intracellular functions in cell stress, proliferation, and differentiation. Given the pattern of DbpA staining in inflammatory glomerular diseases, without adherence to cell boundaries, we hypothesized extracellular protein occurrence and specific functions. Lipopolysaccharide and ionomycin induce DbpA expression and secretion from melanoma and mesangial cells. Unlike its homologue Y-box-binding protein 1 (YB-1), DbpA secretion requires inflammasome activation, as secretion is blocked upon the addition of a NOD-like receptor protein-3 (NLRP3) inhibitor. The addition of recombinant DbpA enhances melanoma cell proliferation, migration, and competes with tumor necrosis factor (TNF) binding to its receptor (TNFR1). In TNF-induced cell death assays, rDbpA initially blocks TNF-induced apoptosis, whereas at later time points (>24 h), cells are more prone to die. Given that CSD proteins YB-1 and DbpA fulfill the criteria of alarmins, we propose that their release signals an inherent danger to the host. Some data hint at an extracellular complex formation at a ratio of 10:1 (DbpA:YB-1) of both proteins. Full article

Invertebrate striated muscle myosin filaments are highly variable in structure. The best characterized myosin filaments are those found in insect indirect flight muscle (IFM) in which the flight-powering muscles are not attached directly to the wings. Four insect orders, Hemiptera, Diptera, Hymenoptera, and Coleoptera, have evolved IFM. IFM thick filaments from the first three orders have highly similar myosin arrangements but differ significantly among their non-myosin proteins. The cryo-electron microscopy of isolated IFM myosin filaments from the Dipteran Drosophila melanogaster described here revealed the coexistence of two distinct filament types, one presenting a tubular backbone like in previous work and the other a solid backbone. Inside an annulus of myosin tails, tubular filaments show no noticeable densities; solid filaments show four paired paramyosin densities. Both myosin heads of the tubular filaments are disordered; solid filaments have one completely and one partially immobilized head. Tubular filaments have the protein stretchin-klp on their surface; solid filaments do not. Two proteins, flightin and myofilin, are identifiable in all the IFM filaments previously determined. In Drosophila, flightin assumes two conformations, being compact in solid filaments and extended in tubular filaments. Nearly identical solid filaments occur in the large water bug Lethocerus indicus, which flies infrequently. The Drosophila tubular filaments occur in younger flies, and the solid filaments appear in older flies, which fly less frequently if at all, suggesting that the solid filament form is correlated with infrequent muscle use. We suggest that the solid form is designed to conserve ATP when the muscle is not in active use. Full article

Introduction: A 51-year-old male was seen complaining of pneumaturia and bowel complaints, including blood per rectum. The patient related a history of an open left inguinal hernia repair utilizing a Kugel mesh ten years before. Case Presentation: Cystoscopy and colonoscopy demonstrated a hemorrhagic mass due to a prosthetic mesh protruding into the bladder and colon. Following colonoscopy and cystoscopy, a large inflammatory mass involving both the colon and urinary bladder was resected, which contained a rolled-up “tubular” mesh structure. After primary repair of the urinary bladder and placement of a Foley catheter, the sigmoid colon and mesh were resected, and the colonic anastomosis was completed. Outcome: Postoperatively, the patient progressed well with normal colon and bladder function after the removal of the Foley catheter. Discussion: Historically, the patient demonstrated the risk of major multiorgan surgical complications of a newer inguinal hernia repair technique, which may occur even a decade or more after the initial surgical correction and is, therefore, presented. Full article

This paper presents the results of research from the renovation of functional parts of crane wheel surfaces. The aim of the research was to verify the possibilities of changing the chemical composition of the additive materials for submerged arc cladding, in order to increase the resistance of the wheel surfaces to wear. The base material of the crane wheel was heat-treated carbon steel for castings, mat. no. 1.0553. The renovation process was carried out on three equal wheels. Conventionally used additive material, the same one used for the interlayer and two covering layers, was used on one wheel. On two other wheels, newly increased tubular wires with a higher proportion of carbide-forming additives (Cr, Mo) were used for the carbide coating of two covering layers, in addition to their conventional additive material. Low-alloy additive material was applied to the newly elevated wires. The quality of the clads was assessed using non-destructive tests. Subsequently, microstructural analysis was carried out on the test samples taken from the renovated wheels, by means of light microscopy. On the cross cuttings, the course of hardness was evaluated using Vickers analysis. The resistance of functional surfaces to adhesion wear was evaluated based on weight losses measured using the AMSler experimental equipment. The results of the experiments showed an increase in the tribological resistance of the surfaces, specifically by 45% due to the newly developed wire C1 and by 18% due to wire B1, and it is therefore possible to recommend renovation. Full article

The construction duration of long-span arch bridges is excessively prolonged due to insufficient closing precision and the non-convergence of traditional cable adjustment calculation methods. This study investigates cable force management in long-span concrete-filled steel tubular (CFST) arch bridges during cable-stayed buckle construction, aiming to improve construction safety and precision in arch rib alignment. Using the Pingnan Third Bridge and Tian’e Longtan Bridge as practical examples, the research develops a multi-objective optimization method for cable forces that integrates influence matrices, constrained minimization, and a forward iterative approach. This method offers a robust strategy for tensioning and cable-stayed buckling, enabling real-time monitoring, calculation, and adjustment during the construction of large-span CFST arch bridges. The results reveal that the iterative approach notably enhances calculation efficiency compared to conventional methods. For instance, field measurements at the Pingnan Third Bridge show a minimal arch closure error of only 3 mm. Additionally, the study addresses concerns about excessive stress in exposed steel tubes during concrete casting. By optimizing the sequence of main arch closure and concrete casting, stress in the exposed steel tube is reduced from 373 MPa to 316 MPa, thus meeting specification requirements. In summary, the multi-objective cable force optimization method demonstrates superior efficiency in determining cable tension and controlling rib alignment during cable-stayed buckle construction of long-span CFST arch bridges. Full article