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20 pages, 2631 KiB  
Article
Effects of High Temperature at Grain Filling Stage on Grain Quality and Gene Transcription in Heat-Sensitive Versus Heat-Tolerant Rice Cultivars
by Yujian Guo, Hui Luo, Jiajie Yi, Yuqi Zhu, Xiaojie Ma, Yubing Jiang, Yanjiao Peng, Yunhua Xiao, Guilian Zhang, Xiong Liu and Huabing Deng
Agronomy 2025, 15(3), 668; https://doi.org/10.3390/agronomy15030668 - 7 Mar 2025
Viewed by 126
Abstract
There are many factors affecting rice yield and quality during cultivation, including temperature, light, water, and fertilization, among which high temperature (HT) is one of the main factors affecting rice yield and quality. However, less is known about the effects and potential mechanisms [...] Read more.
There are many factors affecting rice yield and quality during cultivation, including temperature, light, water, and fertilization, among which high temperature (HT) is one of the main factors affecting rice yield and quality. However, less is known about the effects and potential mechanisms of different durations of HT stress during the grain filling stage on grain quality. In this study, the differences in rice quality and starch rapid viscosity analyzer (RVA) characteristics of eight indica rice varieties under different high-temperature treatment times were studied by simulating high temperature in an artificial climate chamber. The prolonged duration of HT leads to an overall deterioration in the milling quality, appearance quality, and cooking quality of rice. The impact of HT duration on the starch RVA characteristics of rice is more complex and is mainly related to the varieties. Among them, the starch RVA characteristics of R313 were more stable. It is worth noting that there is a significant difference in the sensitivity of the appearance quality of 8XR274 and 5W0076 to HT duration, with 8XR272 being more sensitive and 5W0076 being the opposite. We selected these two varieties for transcriptome analysis after 14 days of HT treatment and found that the number of differentially expressed genes (DEGs) in 8XR274 was significantly less than that in 5W0076. The DEGs of 8XR274 were mainly enriched in pathways related to carbohydrates, while 5W0076 was mainly enriched in pathways related to photosynthesis. Our study provides a new perspective on the molecular response and related genes of different rice varieties under high temperature, as well as the high-quality rice breeding under high temperature. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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17 pages, 1978 KiB  
Review
Multiple Solutions of Ore-Forming Fluids of Carbonate Rock-Related Nephrite Deposits Constrained by Hydrogen and Oxygen Isotopes
by Ping Li, Zongting Liao, Qi Chen, Lijian Qi and Yungui Liu
Minerals 2025, 15(3), 272; https://doi.org/10.3390/min15030272 - 7 Mar 2025
Viewed by 38
Abstract
Hydrogen and oxygen isotopes of ore-forming fluid of nephrite deposits have always been changing due to mixings between different fluids and oxygen isotope exchanges between the ore-forming fluid and country rocks, resulting in that the tremolite (or actinolite) has to constantly re-establish new [...] Read more.
Hydrogen and oxygen isotopes of ore-forming fluid of nephrite deposits have always been changing due to mixings between different fluids and oxygen isotope exchanges between the ore-forming fluid and country rocks, resulting in that the tremolite (or actinolite) has to constantly re-establish new isotope fractionation equilibriums with the dynamic fluid, which is of great significance to understand the genesis of hydrogen and oxygen isotopes of nephrite. Based on this, Taylor’s closed model and fluid mixing model are used to unravel the control of multi-stage evolution of ore-forming fluid on the δD and δ18O of nephrite. Although Taylor’s closed model is conducive to interpreting the genesis of nephrite with light δD and δ18O, such as Vitim nephrite, Russia, and Chuncheon nephrite, South Korea, it is unable to be effectively used in other nephrite. The fluid mixing model can quantitatively constrain proportions of different fluids during different ore-forming stages. Multiple solutions of ore-forming fluids of carbonate rock-related nephrite result from the absence of external constraints, such as isotope compositions of intrusive rocks, carbonate rocks, and meteoric water. Due to the generally heavy δ18O of country rocks, a small amount of meteoric water that enters the hydrothermal system in the later ore-forming stage is insufficient to offset the δ18O increment of nephrite caused by the oxygen isotope exchange between country rocks and water, which should be responsible for the abnormal heavy δ18O of Luodian nephrite, Dahua nephrite, Sanchakou nephrite, Xiaomeiling nephrite, etc., and not metamorphic water dominating their formation. Full article
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17 pages, 3657 KiB  
Article
Influence of Miscanthus floridulus on Heavy Metal Distribution and Phytoremediation in Coal Gangue Dump Soils: Implications for Ecological Risk Mitigation
by Jiaolong Wang, Yan Jiang, Yuanying Peng, Xiaoyong Chen, Wende Yan, Xiaocui Liang, Qian Wu and Jingjie Fang
Plants 2025, 14(6), 836; https://doi.org/10.3390/plants14060836 - 7 Mar 2025
Viewed by 78
Abstract
Coal gangue dumps, a byproduct of coal mining, contribute significantly to heavy metal contamination, impacting soil and water quality. In order to assess the levels of heavy metal contamination in soils at different stages of abandonment, this study investigated the role of Miscanthus [...] Read more.
Coal gangue dumps, a byproduct of coal mining, contribute significantly to heavy metal contamination, impacting soil and water quality. In order to assess the levels of heavy metal contamination in soils at different stages of abandonment, this study investigated the role of Miscanthus floridulus (M. floridulus) in the spatial distribution and remediation of six heavy metals (Cd, Cr, Mn, Ni, Cu, and Pb) in coal gangue dump soils abandoned for 0, 8, and 12 years in Pingxiang City, Jiangxi Province, China. Fieldwork was conducted at three sites operated by the Pingxiang Mining Group: Anyuan (active, barren), Gaokeng (8 years, natural vegetation), and Qingshan (12 years, partially remediated). Anyuan remains largely barren, while Gaokeng supports natural vegetation without formal remediation. In contrast, Qingshan supports diverse plant species, including M. floridulus, due to partial remediation. Using a randomized design, root exudates, heavy metal concentrations, and soil properties were analyzed. The results showed that Cd poses the highest ecological risk, with concentrations of 64.56 mg kg−1 at the active site, 25.57 mg kg−1 at the 8-year site, and 39.13 mg kg−1 at the 12-year site. Cu and Pb showed accumulation, while Cr and Mn decreased over time. Root exudates from M. floridulus enhanced metal bioavailability, influencing Cd, Cr, and Ni concentrations. These findings highlight the importance of rhizosphere processes in metal mobility and inform sustainable remediation strategies for post-mining landscapes. Full article
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20 pages, 2224 KiB  
Article
Sustainable Hydrogen Production from Plastic Waste: Optimizing Pyrolysis for a Circular Economy
by Fiyinfoluwa Joan Medaiyese, Hamid Reza Nasriani, Khalid Khan and Leila Khajenoori
Hydrogen 2025, 6(1), 15; https://doi.org/10.3390/hydrogen6010015 - 7 Mar 2025
Viewed by 160
Abstract
Hydrogen is a clean, non-polluting fuel and a key player in decarbonizing the energy sector. Interest in hydrogen production has grown due to climate change concerns and the need for sustainable alternatives. Despite advancements in waste-to-hydrogen technologies, the efficient conversion of mixed plastic [...] Read more.
Hydrogen is a clean, non-polluting fuel and a key player in decarbonizing the energy sector. Interest in hydrogen production has grown due to climate change concerns and the need for sustainable alternatives. Despite advancements in waste-to-hydrogen technologies, the efficient conversion of mixed plastic waste via an integrated thermochemical process remains insufficiently explored. This study introduces a novel multi-stage pyrolysis-reforming framework to maximize hydrogen yield from mixed plastic waste, including polyethylene (HDPE), polypropylene (PP), and polystyrene (PS). Hydrogen yield optimization is achieved through the integration of two water–gas shift reactors and a pressure swing adsorption unit, enabling hydrogen production rates of up to 31.85 kmol/h (64.21 kg/h) from 300 kg/h of mixed plastic wastes, consisting of 100 kg/h each of HDPE, PP, and PS. Key process parameters were evaluated, revealing that increasing reforming temperature from 500 °C to 1000 °C boosts hydrogen yield by 83.53%, although gains beyond 700 °C are minimal. Higher reforming pressures reduce hydrogen and carbon monoxide yields, while a steam-to-plastic ratio of two enhances production efficiency. This work highlights a novel, scalable, and thermochemically efficient strategy for valorizing mixed plastic waste into hydrogen, contributing to circular economy goals and sustainable energy transition. Full article
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21 pages, 11630 KiB  
Article
Assessment of the Maize Crop Water Stress Index (CWSI) Using Drone-Acquired Data Across Different Phenological Stages
by Mpho Kapari, Mbulisi Sibanda, James Magidi, Tafadzwanashe Mabhaudhi, Sylvester Mpandeli and Luxon Nhamo
Drones 2025, 9(3), 192; https://doi.org/10.3390/drones9030192 - 6 Mar 2025
Viewed by 84
Abstract
The temperature-based crop water stress index (CWSI) is the most robust metric among precise techniques that assess the severity of crop water stress, particularly in susceptible crops like maize. This study used a unmanned aerial vehicle (UAV) to remotely collect data, to use [...] Read more.
The temperature-based crop water stress index (CWSI) is the most robust metric among precise techniques that assess the severity of crop water stress, particularly in susceptible crops like maize. This study used a unmanned aerial vehicle (UAV) to remotely collect data, to use in combination with the random forest regression algorithm to detect the maize CWSI in smallholder croplands. This study sought to predict a foliar temperature-derived maize CWSI as a proxy for crop water stress using UAV-acquired spectral variables together with random forest regression throughout the vegetative and reproductive growth stages. The CWSI was derived after computing the non-water-stress baseline (NWSB) and non-transpiration baseline (NTB) using the field-measured canopy temperature, air temperature, and humidity data during the vegetative growth stages (V5, V10, and V14) and the reproductive growth stage (R1 stage). The results showed that the CWSI (CWSI < 0.3) could be estimated to an R2 of 0.86, RMSE of 0.12, and MAE of 0.10 for the 5th vegetative stage; an R2 of 0.85, RMSE of 0.03, and MAE of 0.02 for the 10th vegetative stage; an R2 of 0.85, RMSE of 0.05, and MAE of 0.04 for the 14th vegetative stage; and an R2 of 0.82, RMSE of 0.09, and MAE of 0.08 for the 1st reproductive stage. The Red, RedEdge, NIR, and TIR UAV-bands and their associated indices (CCCI, MTCI, GNDVI, NDRE, Red, TIR) were the most influential variables across all the growth stages. The vegetative V10 stage exhibited the most optimal prediction accuracies (RMSE = 0.03, MAE = 0.02), with the Red band being the most influential predictor variable. Unmanned aerial vehicles are essential for collecting data on the small and fragmented croplands predominant in southern Africa. The procedure facilitates determining crop water stress at different phenological stages to develop timeous response interventions, acting as an early warning system for crops. Full article
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21 pages, 1770 KiB  
Article
A Cyclic Graywater Treatment Model for Sustainable Wastewater Management Applied in a Small Scale
by Hanen Filali, Malak Moussa, Narcis Barsan, Valentin Nedeff, Oana Irimia and Mohamed Hachicha
Appl. Sci. 2025, 15(5), 2836; https://doi.org/10.3390/app15052836 - 6 Mar 2025
Viewed by 135
Abstract
Water scarcity presents a critical challenge to global sustainability, exacerbated by population growth, climate change, and environmental pollution. In this context, graywater reuse has emerged as a promising solution, offering substantial water savings with significant potential for agricultural applications. However, efficient treatment methods [...] Read more.
Water scarcity presents a critical challenge to global sustainability, exacerbated by population growth, climate change, and environmental pollution. In this context, graywater reuse has emerged as a promising solution, offering substantial water savings with significant potential for agricultural applications. However, efficient treatment methods are essential to ensure safe reuse, as contaminants vary depending on the source. This study introduces a cyclic graywater treatment system that integrates both mechanical and biological filtration processes. A key feature of this system is the inclusion of Chenopodium quinoa, a resilient plant known for its phytoremediation potential, which enhances filtration efficiency and facilitates contaminant removal. The study examines the impact of treated graywater on soil and quinoa properties, focusing on its suitability for irrigation. The results show that the cyclic treatment system significantly improves graywater quality, enhancing the removal of biological and microbiological contaminants, such as BOD, with a significant decrease ranging from 31.33 mg O2/L to 15.74 mg O2/L is observed after treatment. For COD, the average values decreased from 102.64 mg O2/L to 54.19 mg O2/L after treatment, making the treated graywater compliant with Tunisian regulation NT 106.03 and WHO guidelines. Cyclic treatment significantly reduced the microbial load of graywater. For example, for E. coli, the average decreased from 0.87 log 10/100 mL in RGW to 0.58 log 10/100 mL in GWT3. The results demonstrate that the cyclic treatment process can predict the graywater quality beyond the three tested stages. This study highlights the potential of plant-based cyclic graywater treatment systems as an eco-friendly and scalable approach for sustainable water management in agriculture. Full article
(This article belongs to the Special Issue Sustainable Environmental Engineering)
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19 pages, 2268 KiB  
Article
Environmental Assessment of Tannin Extraction from Bark Residues for Application in Water Treatment
by Carla L. Simões, Alice B. P. Santos Neto, Ana C. Rodrigues, Ricardo Ferreira and Ricardo Simoes
Biomass 2025, 5(1), 15; https://doi.org/10.3390/biomass5010015 - 6 Mar 2025
Viewed by 155
Abstract
This study explores the extraction and utilization of tannins from Acacia sp. bark residues for water treatment applications. As a by-product of forest management, Acacia sp. bark is valorized through tannin-based coagulant production, contributing to the circular (bio)economy. A systematic review with bibliometric [...] Read more.
This study explores the extraction and utilization of tannins from Acacia sp. bark residues for water treatment applications. As a by-product of forest management, Acacia sp. bark is valorized through tannin-based coagulant production, contributing to the circular (bio)economy. A systematic review with bibliometric analysis was first conducted to assess the technical–scientific landscape, identifying methodologies and technologies applied to extract and produce natural tannin-based coagulants from Acacia sp. bark residues for water treatment. From the portfolio of analyzed publications, and which followed the thematic axis addressed and the inclusion criteria, only a single study focuses on performing a life cycle assessment (LCA). Due to the relevance of the topic and the clear lack of existing literature, an environmental assessment of the extraction and production of condensed tannins was performed using the LCA methodology from a gate-to-gate perspective. Among the six process stages, spray drying and adsorption (purification) were the primary sources of environmental impact due to their high energy consumption and makeup ethanol use, respectively. The most effective strategy to enhance environmental performance would be reducing water consumption in extraction, thereby lowering energy demand in spray drying. Since both extraction and spray drying require significant energy, decreasing water use and allowing higher moisture content in the condensed tannin extract would mitigate energy consumption. The LCA study thus proved essential in guiding process development toward a reduced environmental footprint. Full article
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17 pages, 769 KiB  
Article
The Role of Foliar-Applied Silicon in Improving the Growth and Productivity of Early Potatoes
by Wanda Wadas and Tomasz Kondraciuk
Agriculture 2025, 15(5), 556; https://doi.org/10.3390/agriculture15050556 - 5 Mar 2025
Viewed by 284
Abstract
Climate change is leading to a decline in global potato production. To ensure food security, it is essential to adapt cultivation practices to the changing climate. The effects of foliar-applied silicon on potato growth and productivity under various hydrothermal conditions were investigated. Potato [...] Read more.
Climate change is leading to a decline in global potato production. To ensure food security, it is essential to adapt cultivation practices to the changing climate. The effects of foliar-applied silicon on potato growth and productivity under various hydrothermal conditions were investigated. Potato plants were treated with three Si-based biostimulants: Actisil (6 g of Si and 20 g of Ca per liter; choline-stabilized orthosilicic acid; Chol-sSa + Ca); Krzemix (6 g of Si per liter; choline-stabilized ammonium metasilicate; Chol-sNH4-Sil); and Optysil (93 g of Si and 24 g of Fe per liter; sodium metasilicate and iron chelate Fe-EDTA; Na-Sil + Fe-EDTA). Biostimulants were foliar-applied twice, at the leaf development stage (BBCH 13–15) and two weeks after the first treatment, at 0.5 L/ha in each treatment. The plants treated with biostimulants were taller and produced greater above-ground biomass and a higher tuber weight than the control plants (without a biostimulant). As a result, the total tuber yield was higher, on average, by 10% to 13% and the marketable tuber yield by 11% to 15%. The plant-growth-promoting and yield-increasing effects of the Si-based biostimulants depended on the hydrothermal conditions during potato growth. Chol-sSA + Ca (Actisil) applications were the most effective. Na-Sil + Fe-EDTA (Optysil) produced better results during a warm and very dry year, while Chol-sNH4-Sil (Krzemix) was effective during colder years with a periodic water deficit. Silicon foliar application can be a new method for increasing early crop potato yields under water shortage conditions. Full article
(This article belongs to the Special Issue The Role of Silicon in Improving Crop Growth Under Abiotic Stress)
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28 pages, 7947 KiB  
Article
Evaluation of Kabuli Chickpea Genotypes for Terminal Drought Tolerance in Tropical Growing Environment
by Megha Subedi, Mani Naiker, Ryan du Preez, Dante L. Adorada and Surya Bhattarai
Plants 2025, 14(5), 806; https://doi.org/10.3390/plants14050806 - 5 Mar 2025
Viewed by 138
Abstract
Terminal drought is the major constraint for chickpea production, leading to yield losses of up to 90% in tropical environments. Understanding the morphological, phenological, and physiological traits underlying drought tolerance is crucial for developing resilient chickpea genotypes. This study elucidates the drought-tolerant traits [...] Read more.
Terminal drought is the major constraint for chickpea production, leading to yield losses of up to 90% in tropical environments. Understanding the morphological, phenological, and physiological traits underlying drought tolerance is crucial for developing resilient chickpea genotypes. This study elucidates the drought-tolerant traits of eight kabuli chickpea genotypes under a controlled environment using polyvinyl chloride (PVC) lysimeters. Terminal drought was imposed after the flowering stage, and the response was assessed against non-stress (well-watered) treatment. Drought stress significantly impacted gas-exchange parameters, reducing the stomatal conductance (16–35%), chlorophyll content (10–22%), carbon assimilation rate (21–40%) and internal carbon concentration (7–14%). Principal component analysis (PCA) indicated three groups among these eight genotypes. The drought-tolerant group included two genotypes (AVTCPK#6 and AVTCPK#19) with higher water use efficiency (WUE), deep-rooted plants, longer maturity, and seed yield stability under drought stress. In contrast, the drought-susceptible group included two genotypes (AVTCPK#1 and AVTCPK#12) that were early-maturing and low-yielding with poor assimilation rates. The intermediate group included four genotypes (AVTCPK#3, AVTCPK8, AVTCPK#24, and AVTCPK#25) that exhibited medium maturity and medium yield, conferring intermediate tolerance to terminal drought. A significantly strong positive correlation was observed between seed yield and key physiological traits (stomatal conductance (gsw), leaf chlorophyll content (SPAD) and carbon assimilation rate (Asat)) and morphological traits (plant height, number of pods, and root biomass). Conversely, carbon discrimination (Δ13C) and intrinsic WUE (iWUE) showed a strong negative correlation with seed yield, supporting Δ13C as a surrogate for WUE and drought tolerance and a trait suitable for the selection of kabuli chickpea genotypes for drought resilience. Full article
(This article belongs to the Section Horticultural Science and Ornamental Plants)
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26 pages, 7119 KiB  
Article
Simultaneous Electrochemical Removal of Selenium and Strontium from Aqueous Solution
by Katarina Licht, Ivan Halkijević, Hana Posavčić and Domagoj Nakić
Appl. Sci. 2025, 15(5), 2786; https://doi.org/10.3390/app15052786 - 5 Mar 2025
Viewed by 210
Abstract
This study investigated the removal of selenium (Se) and strontium (Sr) from water using a three-stage electrochemical reactor with integrated pH control. A total of 102 experiments were performed following a Box–Behnken design that varied the electrode material, applied current, number of electrodes, [...] Read more.
This study investigated the removal of selenium (Se) and strontium (Sr) from water using a three-stage electrochemical reactor with integrated pH control. A total of 102 experiments were performed following a Box–Behnken design that varied the electrode material, applied current, number of electrodes, operating time, and initial pH to evaluate their effects on the Se and Sr removal efficiencies. The complete removal of Se was achieved under multiple conditions, even without pH control, while effective Sr removal required a high current and initial pH adjustment. The top performance for both elements was achieved with a 25 A current, four Fe electrodes, 15 min of operational time per phase (cycle), and a middle range of pH values, which resulted in 97.92% and 99.96% removals of Sr and Se, respectively. This research highlighted the novel approach of using electrochemical pH control to achieve high removal efficiencies of Se and Sr from water in a short operating time, which surpassed the efficiencies reported in previous studies. Full article
(This article belongs to the Special Issue New Approaches to Water Treatment: Challenges and Trends)
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10 pages, 636 KiB  
Article
Competitiveness Strategies and Technical Innovations in Light-Water Small Modular Reactor Projects
by Ludwik Pieńkowski
Energies 2025, 18(5), 1268; https://doi.org/10.3390/en18051268 - 5 Mar 2025
Viewed by 180
Abstract
It is widely recognized that economies of scale enhance the competitiveness of large-scale nuclear reactors compared to light-water small modular reactors (SMRs). As such, choosing an appropriate strategy to enhance competitiveness is crucial for the future of SMRs. Their development is still in [...] Read more.
It is widely recognized that economies of scale enhance the competitiveness of large-scale nuclear reactors compared to light-water small modular reactors (SMRs). As such, choosing an appropriate strategy to enhance competitiveness is crucial for the future of SMRs. Their development is still in the early stages, and among the leading projects, two distinct approaches to technical innovation can be observed. In some projects, technical innovations are rejected because they are perceived as triggers for risky, costly, and long-term processes. In short, this means that the competitive advantage is based primarily on modular design and the benefits of long production runs, which might require at least a few successful implementations. Examples of this approach include the Westinghouse AP300 and Rolls-Royce SMR designs. In other projects, technical innovations are viewed as a means to achieve substantial cost reductions. Here, the initial challenge is to prove that the proposed solutions are safe. Next, it must be demonstrated that their implementation and operation meet the designers’ expectations. These goals can be achieved with the first implementation. Such an approach is exemplified, for instance, in the NuScale and GEH BWRX-300 projects. Currently, available economic analyses show that it is challenging not only to identify the most promising SMR projects but also to determine which approach to technical innovation will ultimately be more effective. Therefore, it is worth examining how leading SMR projects have improved their competitiveness. Additionally, it is important to remember that, even if light-water SMRs are not deployed, it is likely that some of their innovative solutions will be incorporated into other advanced nuclear power plant designs and potentially applied beyond the nuclear industry. Full article
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12 pages, 1513 KiB  
Article
Identification of Different Ecomorphotypes of Coilia nasus in the Dawanzhou Section of the Yangtze River
by Jiahao Liu, Tao Jiang, Junren Xue, Xiubao Chen, Hongbo Liu and Jian Yang
Fishes 2025, 10(3), 113; https://doi.org/10.3390/fishes10030113 - 5 Mar 2025
Viewed by 88
Abstract
X-ray electron probe microanalyzer technology was used to study the microchemistry and habitat history of Coilia nasus collected from the Dawanzhou section of the Yangtze River between May and June 2023. The Sr/Ca ratio from the otolith core to the otolith diameter was [...] Read more.
X-ray electron probe microanalyzer technology was used to study the microchemistry and habitat history of Coilia nasus collected from the Dawanzhou section of the Yangtze River between May and June 2023. The Sr/Ca ratio from the otolith core to the otolith diameter was low (640–1100 µm), representing the first stage of development. In the second stage, C. nasus exhibited two distinct types. The first type, which included individuals 5HK05 and 6HK03, exclusively inhabited brackish estuarine waters. The second type, comprising 13 individuals, resided in higher-salinity seawater environments (Sr/Ca > 7). Furthermore, individuals 5HK01, 5HK03, 5HK07, and 6HK05 displayed a phase with a high Sr/Ca ratio compared to other fish. Freshwater coefficient analysis indicated that C. nasus in the Dawanzhou water area was unlikely to continue upstream to Dongting Lake in the middle reaches of the Yangtze River but may have entered Poyang Lake through its mouth or reproduced in its upper reaches. Analysis of sexual maturity and migration history suggested that the Dawanzhou section primarily serves as a migration channel for C. nasus, with a potential spawning ground for this high-value fish located nearby. Full article
(This article belongs to the Section Biology and Ecology)
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25 pages, 3629 KiB  
Article
Exploring the Diversity of Microbial Communities Associated with Two Anopheles Species During Dry Season in an Indigenous Community from the Colombian Amazon
by Daniela Duque-Granda, Rafael José Vivero-Gómez, Laura Alejandra González Ceballos, Howard Junca, Santiago R. Duque, María Camila Aroca Aguilera, Alejandro Castañeda-Espinosa, Gloria Cadavid-Restrepo, Giovan F. Gómez and Claudia Ximena Moreno-Herrera
Insects 2025, 16(3), 269; https://doi.org/10.3390/insects16030269 - 4 Mar 2025
Viewed by 310
Abstract
Malaria disease affects millions of people annually, making the Amazon Basin a major hotspot in the Americas. While traditional control strategies rely on physical and chemical methods, the Anopheles microbiome offers a promising avenue for biological control, as certain bacteria can inhibit parasite [...] Read more.
Malaria disease affects millions of people annually, making the Amazon Basin a major hotspot in the Americas. While traditional control strategies rely on physical and chemical methods, the Anopheles microbiome offers a promising avenue for biological control, as certain bacteria can inhibit parasite development and alter vector immune and reproductive systems, disrupting the transmission cycle. For this reason, this study aimed to explore the bacterial communities in An. darlingi and An. triannulatus s.l., including breeding sites, immature stages, and adults from San Pedro de los Lagos (Leticia, Amazonas) through next-generation sequencing of the 16S rRNA gene. The results revealed a higher bacterial genus richness in the L1–L2 larvae of An. triannulatus s.l. Aeromonas and Enterobacter were prevalent in most samples, with abundances of 52.51% in L3–L4 larvae and 48.88% in pupae of An. triannulatus s.l., respectively. In breeding site water, Verrucomicrobiota bacteria were the most dominant (52.39%). We also identified Delftia (15.46%) in An. triannulatus s.l. pupae and Asaia (98.22%) in An. darlingi, linked to Plasmodium inhibition, and Elizabethkingia, in low abundances, along with Klebsiella and Serratia, known for paratransgenesis potential. Considering the high bacterial diversity observed across the different mosquito life stages, identifying bacterial composition is the first step towards developing new strategies for malaria control. However, the specific roles of these bacteria in anophelines and the malaria transmission cycle remain to be elucidated. Full article
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28 pages, 13856 KiB  
Article
Utilizing Multi-Omics Analysis to Elucidate the Molecular Mechanisms of Oat Responses to Drought Stress
by Xiaojing Chen, Jinghui Liu, Baoping Zhao, Junzhen Mi and Zhongshan Xu
Plants 2025, 14(5), 792; https://doi.org/10.3390/plants14050792 - 4 Mar 2025
Viewed by 107
Abstract
The oat is a crop and forage species with rich nutritional value, capable of adapting to various harsh growing environments, including dry and poor soils. It plays an important role in agricultural production and sustainable development. However, the molecular mechanisms underlying the responses [...] Read more.
The oat is a crop and forage species with rich nutritional value, capable of adapting to various harsh growing environments, including dry and poor soils. It plays an important role in agricultural production and sustainable development. However, the molecular mechanisms underlying the responses of oat to drought stress remain unclear, warranting further research. In this study, we conducted a pot experiment with the drought-resistant cultivar JiaYan 2 (JIA2) and water-sensitive cultivar BaYou 9 (BA9) during the booting stage under three water gradient treatment conditions: 30% field capacity (severe stress), 45% field capacity (moderate stress), and 70% field capacity (normal water supply). After 7 days of stress, root samples were collected for transcriptome and proteome analyses. Transcriptome analysis revealed that under moderate stress, JIA2 upregulated 1086 differential genes and downregulated 2919 differential genes, while under severe stress, it upregulated 1792 differential genes and downregulated 4729 differential genes. Under moderate stress, BA9 exhibited an upregulation of 395 differential genes, a downregulation of 669, and an upregulation of 886 differential genes, and it exhibited 439 downregulations under severe stress. Under drought stress, most of the differentially expressed genes (DEGs) specific to JIA2 were downregulated, mainly involving redox reactions, carbohydrate metabolism, plant hormone signal regulation, and secondary metabolism. Proteomic analysis revealed that in JIA2, under moderate stress, 489 differential proteins were upregulated and 394 were downregulated, while 493 differential proteins were upregulated and 701 were downregulated under severe stress. In BA9, 590 and 397 differential proteins were upregulated under moderate stress, with 126 and 75 upregulated differential proteins under severe stress. Correlation analysis between transcriptomics and proteomics demonstrated that compared with no drought stress, four types of differentially expressed proteins (DEPs) were identified in the JIA2 differential gene–protein interaction network analysis under severe stress. These included 13 key cor DEGs and DEPs related to plant hormone signal transduction, biosynthesis of secondary metabolites, carbohydrate metabolism processes, and metabolic pathways. The consistency of gene and protein expression was validated using qRT-PCR, indicating their key roles in the strong drought resistance of JIA2. Full article
(This article belongs to the Section Plant Molecular Biology)
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13 pages, 4529 KiB  
Article
Graft Reaction of Furfural with Polyvinyl Chloride and Its Effect on Thermal Stability of Polyvinyl Chloride
by Mengying Kou and Kanshe Li
Organics 2025, 6(1), 12; https://doi.org/10.3390/org6010012 - 4 Mar 2025
Viewed by 123
Abstract
A graft reaction of polyvinyl chloride (PVC) with furfural was conducted in a tetrahydrofuran solution. The resulting graft structure (FF-g-PVC) was characterized using UV spectroscopy, photoluminescence (PL), Fourier Transform Infrared (FTIR) spectroscopy, Raman spectroscopy, and proton nuclear magnetic resonance (1H NMR) [...] Read more.
A graft reaction of polyvinyl chloride (PVC) with furfural was conducted in a tetrahydrofuran solution. The resulting graft structure (FF-g-PVC) was characterized using UV spectroscopy, photoluminescence (PL), Fourier Transform Infrared (FTIR) spectroscopy, Raman spectroscopy, and proton nuclear magnetic resonance (1H NMR) spectroscopy. The grafting efficiency was determined through ultraviolet spectrophotometry. Thermal stability analysis via thermogravimetric (TG) testing revealed that furfural was successfully grafted onto the PVC chain. In a nitrogen atmosphere, the temperature of the maximum weight loss rate during the first stage of pyrolysis increased from 296.3 °C to 301.7 °C, while the activation energy for the second stage increased from 199.4 kJ/mol to 294.4 kJ/mol, indicating enhanced stability and delayed degradation of the PVC. Additionally, microwave irradiation markedly improved the graft reaction, achieving a grafting rate of 57.76‰ compared to only 1.808‰ with water bath heating. The optimal conditions were found to be a PVC/FF/Zn ratio of 1:1:0.9, with microwave irradiation for 20 min at 40 °C. Full article
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