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This protocol covers the production and construction of new-generation transmorphic phage/adeno-associated virus vectors for the systemic delivery of nucleic acid payloads. In vitro and in vivo applications of transmorphic phage/adeno-associated virus particles are also discussed.
This protocol for the precise tracking of nanoparticles in plant roots uses CLSM for macroscopic tissue examination and TEM for cellular-level analysis and provides methodologies for preparing plant materials before imaging.
DiMeLo-seq uses long-read, single-molecule sequencing to map proteinâDNA interactions genome wide. This allows mapping of multiple interaction sites on single DNA molecules and profiling protein binding in the context of endogenous DNA methylation.
The three methods present visualize epigenetic modifications and their spatial proximities in single cells; base-encoded amplifying FISH, pairwise proximity-differentiated amplifying FISH and cellular macromolecules-tethered DNA walking indexing.
This protocol extension details two approaches to creating a bioswitchable delivery system for microRNA therapeutics, based on a tetrahedral DNA nanostructure with a ribonuclease H-sensitive sequence as a bioswitchable apparatus for the controlled release of cargo.
High-resolution diffusion uses a dense optical flow algorithm to quantify and classify the motion of nuclear macromolecules, assigning biophysical parameters such as the diffusion constant, the anomalous exponent and the drift velocity.
STRAIGHT-IN is a platform for rapidly generating panels of genetically modified human pluripotent stem cell lines through targeted integration of a DNA payload into a landing pad, which is achieved with minimal scarring.
Nitrogen heterocycles are a large class of chemically and biologically relevant compounds. This protocol describes their synthesis from alcohols using homogeneous iridium/ruthenium catalysts and heterogeneous cobalt/copper catalysts.
Native mass spectrometry can be combined with lipidomic experiments to determine the structural and functional lipids of receptor and transporter assemblies. This protocol describes how to use initial native mass spectrometry results to guide experimental design.
This protocol describes the generation of cyborg bacterial cells by using intracellular hydrogelation. These nondividing, stressor-resistant engineered bacteria chassis can find application as living environmental biosensors and in disease treatment.
Developing optimal nanozymes requires standardized methods for measuring their catalytic activity and reaction kinetics. This protocol integrates enzyme based MichaelisâMenten kinetics with measured physical properties and computational methods.
Proximity sequencing uses a panel of antibodies to probe single cells for up to hundreds of targets simultaneously. The approach is capable of detecting targets that are located within 50â70 nm of each other and thus likely to form complexes.
nPOP is a method for parallel preparation of thousands of single cells in nanoliter-volume droplets deposited on glass slides by using the CellenONE instrument. This protocol describes the liquid handling for multiplexed mass spectrometry proteomics.
This protocol describes a flux synthesis approach for two-dimensional covalent organic frameworks. Compared with other approaches, this method does not use solvents, making it environmentally friendly, and is scalable up to the kilogram scale.
BANC-seq enables quantification of genome-wide transcription factor binding affinities in the native chromatin context. This protocol describes implementations based on chromatin immunoprecipitation or cleavage under target and release using nuclease, followed by library preparation, sequencing and data analysis.
The transplantation of human cortical organoids in rats enables maturation and integration of human neural cells that can engage with the host circuitry, providing a framework to study alterations in morphology and physiology of patient-derived tissue.
This protocol describes the use of iPhone LiDAR to generate high-resolution 3D models for topographic surveying and to measure their changes over time. The protocol simplifies the use of the iPhone LiDAR sensor, making it accessible to non-experts.
This protocol describes a comprehensive framework for applying network control theory to the human structural connectome to study its topology and show how that topology affects the dynamics of neural activity states, using a software package written in Python.
This protocol describes the in vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis. Given the ethical limitations restricting the use of human embryos beyond 14 days, nonhuman primate embryos are an ideal model.
Engineered and anthropogenic nanoscale materials in the environment acquire a coating of biomolecules (biocorona) that modulates their properties, uptake and biodistribution. This protocol streamlines biocorona analysis to support the development of safe and sustainable nanotechnology.