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ABSTRACT Introduction: The main purpose of aerobic exercise is to enhance cardiopulmonary endurance, so it is necessary to build cardiopulmonary endurance response models based on different frequencies of aerobic exercise. Objective: To study the cardiopulmonary endurance response of women to different frequencies of aerobic exercise. Methods: Twenty young female desk workers (female teachers and civil servants) who worked out at a fitness club were randomly divided into two groups. Cardiopulmonary function, both before and after 16 weeks of aerobic exercise at different exercise loads, was studied and analyzed. Results: After 16 weeks of aerobic exercise at different exercise loads, all the young women had significantly improved their vital capacity (VC), and their maximum oxygen uptake ability was improved to a certain extent. Compared with the 45-minute aerobic exercise group, the vital capacity (VC)of 90-minute aerobic exercise group was significantly increased (P>0.05). Conclusions: When performed at a consistent frequency level, aerobic exercise with a relatively high exercise load can better develop the body’s respiratory system function. This may be due to deep stimulation of the respiratory system from high-load aerobic exercise, and ultimately to the intensive exercising of lung function. Level of evidence II; Therapeutic studies - investigation of treatment results.

The objective of the current research is to validate the microbiological quality of Emitron against MS2 phage, Corona virus, bacteria, yeast, and molds. Unimech healthcare designed PMEE based Emitron which is mostly used to reduce the microbial count and virus count in an area. To perform microbiological testing of the Emitron, environmental monitoring was performed for MS2 phage, Corona virus, bacteria, yeast, and molds before and after treatment by settle plate method. Petri plates of sterilized Phage agar, SCDA and PDA media were prepared and exposed for environment monitoring before and after treatment by settle plate method. The phage agar and SCDA plates were incubated at 37°C for 72 hours and PDA plates incubated at 25°C for 5 days. Total of 99% reduction was reported in microbiology lab with respect to the microorganisms after installing Emitron. We can conclude that the Unimech healthcare’s Emitron is virus attenuation device so one can use it to purify the air and kill the viruses, bacteria, fungi, and other microbes.

ABSTRACT Introduction: Using gene therapy to transfer specific genes to implant therapeutic proteins into damaged tissues is a more promising way to treat sports injuries. The combination of tissue engineering and gene therapy will potentially promote the regeneration and repair of various damaged tissues. Objective: This article explores the adaptive relationship between gene selection therapy and athletes in sports. Methods: We selected students of related majors in sports schools to conduct specific genetic testing and measure the muscle area, fatigue level, muscle damage, and other related indicators before and after exercise. Results: After a series of physical fitness assessments, an increase in the gene sequence, as well as changes in the biochemical indices, were confirmed Conclusions: The muscle gain of the test subject during training is better than other genotypes. Level of evidence II; Therapeutic studies - investigation of treatment results.

The objective of the current research is to validate the microbiological quality of Emitron against MS2 phage, Corona virus, bacteria, yeast, and molds. Unimech healthcare designed PMEE based Emitron which is mostly used to reduce the microbial count and virus count in an area. To perform microbiological testing of the Emitron, environmental monitoring was performed for MS2 phage, Corona virus, bacteria, yeast, and molds before and after treatment by settle plate method. Petri plates of sterilized Phage agar, SCDA and PDA media were prepared and exposed for environment monitoring before and after treatment by settle plate method. The phage agar and SCDA plates were incubated at 37°C for 72 hours and PDA plates incubated at 25°C for 5 days. Total of 99% reduction was reported in microbiology lab with respect to the microorganisms after installing Emitron. We can conclude that the Unimech healthcare’s Emitron is virus attenuation device so one can use it to purify the air and kill the viruses, bacteria, fungi, and other microbes.

Abstract The essential oil of citronella (Cymbopogon winterianus) has several biological activities, among them the insect repellent action. Some studies showed that cinnamic acid esters can be applied as natural pesticides, insecticides and fungicides. In this context, the objective of the present work was to evaluate the production of esters from citronella essential oil with cinnamic acid via enzymatic esterification. Besides, the essential oil toxicity before and after esterification against Artemia salina and larvicidal action on Aedes aegypti was investigated. Esters were produced using cinnamic acid as the acylating agent and citronella essential oil (3:1) in heptane and 15 wt% NS 88011 enzyme as biocatalysts, at 70 °C and 150 rpm. Conversion rates of citronellyl and geranyl cinnamates were 58.7 and 69.0% for NS 88011, respectively. For the toxicity to Artemia salina LC50 results of 5.29 μg mL-1 were obtained for the essential oil and 4.36 μg mL-1 for the esterified oils obtained with NS 88011. In the insecticidal activity against Aedes aegypti larvae, was obtained LC50 of 111.84 μg mL-1 for the essential oil of citronella and 86.30 μg mL-1 for the esterified oils obtained with the enzyme NS 88011, indicating high toxicity of the esters. The results demonstrated that the evaluated samples present potential of application as bioinsecticide.