oroxylum indicum
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Oroxylum indicum, of the Bignoniaceae family, has various ethnomedical uses such as an astringent, anti-inflammatory, anti-bronchitis, anti-helminthic and anti-microbial, including anticancer properties. The druggability of OI stem bark extract was determined by its molecular docking interactions with PARP and Caspase-3, two proteins involved in cell survival and death. Note that 50 µg/mL of Oroxylum indicum extract (OIE) showed a significant (p < 0.05%) toxicity to HSC-3 cells. MTT aided cell viability and proliferation assay demonstrated that 50 µg/mL of OIE displayed significant (p < 0.5%) reduction in cell number at 4 h of incubation time. Cell elongation and spindle formation was noticed when HSC-3 cells were treated with 50 µg/mL of OIE. OIE initiated DNA breakage and apoptosis in HSC-3 cells, as evident from DNA ladder assay and calcein/EB staining. Apoptosis potential of OIE is confirmed by flow cytometer and triple-staining (live cell/apoptosis/necrosis) assay. Caspase-3/7 fluorescence quenching (LANCE) assay demonstrated that 50 µg/mL of OIE significantly enhanced the RFU of caspases-3/7, indicating that the apoptosis potential of OIE is probably through the activation of caspases. Immuno-cytochemistry of HSC-3 cells treated with 50 µg/mL of OIE showed a significant reduction in mitochondrial bodies as well as a reduction in RFU in 60 min of incubation time. Immunoblotting studies clearly showed that treatment of HSC-3 cells with OI extract caused caspase-3 activation and PARP deactivation, resulting in apoptotic cell death. Overall, our data indicate that OIE is an effective apoptotic agent for human squamous carcinoma cells and it could be a future cancer chemotherapeutic target.

Root bark of sonapatha is an astringent, tonic, anti-diarrhoeal, diuretic, anodyne, and is used to cure dropsy. It is an ingredient of ‘dashamoolarishta’ of Ayurvedic medicine. Stem bark is anti-rheumatic. An infusion of bark powder is diaphoretic. Tender fruits have spas- molytic, carminative, and stomachic properties, while seeds are purgative.it is a medium-sized, soft-wooded tree attaining a height of 10–16 m. Stem bark is dull brown in colour; leaves are broad, 60–120 cm in length and pinnately compound. Leaflets are ovate, wavy, and acuminate. Leaf fall occurs during winter season (January) each year. The tree is recognized by ternately bipinnate leaves. The root bark contains chrysin, baicalein, dehydrobaicalein, and orozylin. Stem bark possesses flavonoids such as oroxylin, baicalein, scutelarin and 7-rutinoside, chrysin, and p-coumaric acid. Heartwood yields β-sitosterol and isoflavone-prunetin. Root bark of sonapatha is an astringent, tonic, anti-diarrhoeal, diuretic, anodyne, and is used to cure dropsy. It is an ingredient of ‘dashamoolarishta’ of Ayurvedic medicine. Stem bark is anti-rheumatic. An infusion of bark powder is diaphoretic. Tender fruits have spas- molytic, carminative, and stomachic properties, while seeds are purgative. It is a medium-sized, soft-wooded tree attaining a height of 10–16 m. Stem bark is dull brown in colour; leaves are broad, 60–120 cm in length and pinnately compound. Leaflets are ovate, wavy, and acuminate. Leaf fall occurs during winter season (January) each year. The tree is recognized by ternately bipinnate leaves. The root bark contains chrysin, baicalein, dehydrobaicalein, and orozylin. Stem bark possesses flavonoids such as oroxylin, baicalein, scutelarin.

Background: Oroxylum indicum has been used in traditional Ayurvedic medicine for the prevention and treatment of several diseases and may have neuroprotective effects.Purpose: Examine the effects of Oroxylum indicum on cognitive function in older adults with self-reported cognitive complaints.Study Design: Two-arm, parallel-group, 12-week, randomized, double-blind, placebo-controlled trial.Methods: Eighty-two volunteers received either 500 mg, twice daily of a standardized Oroxylum indicum extract or placebo. Outcome measures included several computer-based cognitive tasks, the Control, Autonomy, Self-Realization, and Pleasure scale (CASP-19), Cognitive Failures Questionnaire (CFQ), and the Montreal Cognitive Assessment (MoCA). Changes in the concentration of brain-derived neurotrophic factor (BDNF) were also examined.Results: Compared to the placebo, Oroxylum indicum was associated with greater improvements in episodic memory, and on several computer-based cognitive tasks such as immediate word recall and numeric working memory, and a faster rate of learning on the location learning task. However, there were no other significant differences in performance on the other assessed cognitive tests, the MoCA total score, or other self-report questionnaires. BDNF concentrations increased significantly in both groups, with no statistically-significant between-group differences. Oroxylum indicum was well tolerated except for an increased tendency for mild digestive complaints and headaches.Conclusion: The results of this first human trial on the cognitive-enhancing effects of Oroxylum indicum suggest that it is a promising herbal candidate for the improvement of cognitive function in older adults with self-reported cognitive complaints.

Bungli (Oroxylum indicum) has been used for generations in Asia as traditional medicine. The seeds of the bungli are commonly used as traditional medicine to treat typhoid, cough, hypertension, internal fever, fever and diarrhea. The active compounds in this plant are known to have anti-inflammatory, antirheumatic, antifungal and antibacterial activities. Our research on methanol extract of bungli fruit seeds showed its potential to inhibit the growth of Escherichia coli bacteria that causes diarrhea. Bungli seed extract samples with a concentration of 25% showed an inhibition zone with a diameter of 9.22 mm, while at a concentration of 50% the diameter was 11.16 mm and 75% had a diameter of 12.88 mm. This size is indeed smaller than chloramphenicol (26 mm), but this potential can be greater if the extraction is carried out more intensively to extract the active compound, the sample concentration is increased, and the part or age of the plant with the highest content of the active compound is used; so that the Bungli plant is expected to be an alternative in overcoming Escherichia coli infection.

While chemotherapy is the most effective therapeutic approach for treating a variety of cancer patients, commonly used chemotherapeutic agents, often induce several adverse effects. Escalating evidence indicates that chemotherapeutics, particularly doxorubicin (DOX) and cyclophosphamide (CPS), induce cognitive impairment associated with central nervous system toxicity. This study was performed to determine neuroprotective effects of Oroxylum indicum extract (OIE) in regard to preventing chemotherapy induced cognitive impairment (CICI) occurring after 4 cycles of DOX (2mg/kg) and CPS (50mg/kg) combination chemotherapy in male C57BL/6J mice. OIE significantly prevented the chemotherapy impaired short-term cognitive performance, exploratory behavior associated with cognitive performance, cognitive performance, and spatial learning and memory in the Y-maze, Open-Field, Novel Object Recognition, and Morris Water Maze tests, respectively. These data suggest that OIE protects from the CICI. OIE decreased the reactive oxygen species and lipid peroxide generated by the chemotherapy treatment in the brain, while also blocking the chemotherapy-induced glutathione depletion. These results establish that OIE exhibits potent antioxidant activity in chemotherapy treated mice. Notably, OIE significantly increased the Complex-I and Complex-IV activities in the brain, indicating that OIE enhances mitochondrial function in the brain. In silico analysis of the major active chemical constituents (Oroxylin A, Baicalein and Chrysin) of OIE indicated that OIE has a favorable absorption, distribution, metabolism and excretion (ADME) profile. Taken together, our results are consistent with the conclusion that OIE prevents CICI by counteracting oxidative stress and perhaps by improving mitochondrial function.