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AbstractThe potential for a Trichoderma-based compost activator was tested for in-situ rice straw decomposition, under both laboratory and field conditions. Inoculation of Trichoderma caused a 50% reduction in the indigenous fungal population after 2 weeks of incubation for both laboratory and field experiments. However, the Trichoderma population declined during the latter part of the incubation. Despite the significant reduction in fungal population during the first 2 weeks of incubation, inoculated samples were found to have higher indigenous and total fungal population at the end of the experiments with as much as a 300% increase in the laboratory experiment and 50% during day-21 and day-28 samplings in the field experiment. The laboratory incubation experiment revealed that inoculated samples released an average of 16% higher amounts of CO2 compared to uninoculated straw in sterile soil samples. Unsterile soil inoculated with Trichoderma released the highest amount of CO2 in the laboratory experiment. In the field experiment, improved decomposition was observed in samples inoculated with Trichoderma and placed below ground (WTBG). From the initial value of around 35%, the C content in WTBG was down to 28.63% after 42 days of incubation and was the lowest among treatments. This is significantly lower compared with NTBG (No Trichoderma placed below ground, 31.1% C), WTSS (With Trichoderma placed on soil surface, 33.83% C), and NTSS (No Trichoderma placed on soil surface, 34.30% carbon). The WTBG treatment also had the highest N content of 1.1%. The C:N ratio of WTBG was only 26.27, 39.51% lower than the C:N ratio of NTBG, which is 43.43. These results prove that the Trichoderma-based inoculant has the potential to hasten the decomposition of incorporated rice straw.

AbstractOld forests containing ancient trees are essential ecosystems for life on earth. Mechanisms that happen both deep in the root systems and in the highest canopies ensure the viability of our planet. Old forests fix large quantities of atmospheric CO2, produce oxygen, create micro-climates and irreplaceable habitats, in sharp contrast to young forests and monoculture forests. The current intense logging activities induce rapid, adverse effects on our ecosystems and climate. Here we review large old trees with a focus on ecosystem preservation, climate issues, and therapeutic potential. We found that old forests continue to sequester carbon and fix nitrogen. Old trees control below-ground conditions that are essential for tree regeneration. Old forests create micro-climates that slow global warming and are irreplaceable habitats for many endangered species. Old trees produce phytochemicals with many biomedical properties. Old trees also host particular fungi with untapped medicinal potential, including the Agarikon, Fomitopsis officinalis, which is currently being tested against the coronavirus disease 2019 (COVID-19). Large old trees are an important part of our combined cultural heritage, providing people with aesthetic, symbolic, religious, and historical cues. Bringing their numerous environmental, oceanic, ecological, therapeutic, and socio-cultural benefits to the fore, and learning to appreciate old trees in a holistic manner could contribute to halting the worldwide decline of old-growth forests.

Abstract Chilling is an environmental phenomenon that hampers the plant growth. Related studies are mainly on based on the aerial plant parts. While, below ground to rhizosphere microbiome have been neglected under low temperature stress. The overexpression of Lsi1 in Dular significantly enhances its proline concentration compared with wild type Dular. This overexpression phenomenon maintains the osmotic balance of cells through influx of Ca2+, K+, H+ and efflux of Na+. The calcium deposition and the activity of plasma membrane H+-ATPase determined in root tip was consistent with the obtained ion flux results. In addition, the high-throughput sequencing results showed significant variation among identified 84 genera in different rhizocompartments (rhizosphere, rhizoplane and endosphere). The identified bacteria were associated with photosynthesis, energy metabolism, nitrogen fixation and defense, which were significantly increased in overexpressed plant. While, the number of pathogenic microorganisms associating were significantly reduced. Our findings suggests that the stability of ion balance in overexpressed plant roots affects the structure of microbial community in soil. To conclude, the over expression of Lsi1 gene enhanced cold tolerance of rice and its underlying mechanisms were comprehensively analyzed which can further impart its role in the stress tolerant rice plants.

AbstractRapid climate change imperils many small-ranged endemic species as the climate envelopes of their native ranges shift poleward. In addition to abiotic changes, biotic interactions are expected to play a critical role in plant species’ responses. Below-ground interactions are of particular interest given increasing evidence of microbial effects on plant performance and the prevalence of mycorrhizal mutualisms. We used greenhouse mesocosm experiments to investigate how natural northward migration/assisted colonization of Rhododendron catawbiense, a small-ranged endemic eastern U.S. shrub, might be influenced by novel below-ground biotic interactions from soils north of its native range, particularly with ericoid mycorrhizal fungi (ERM). We compared germination, leaf size, survival, and ERM colonization rates of endemic R. catawbiense and widespread R. maximum when sown on different soil inoculum treatments: a sterilized control; a non-ERM biotic control; ERM communities from northern R. maximum populations; and ERM communities collected from the native range of R. catawbiense. Germination rates for both species when inoculated with congeners' novel soils were significantly higher than when inoculated with conspecific soils, or non-mycorrhizal controls. Mortality rates were unaffected by treatment, suggesting that the unexpected reciprocal effect of each species’ increased establishment in association with heterospecific ERM could have lasting demographic effects. Our results suggest that seedling establishment of R. catawbiense in northern regions outside its native range could be facilitated by the presence of extant congeners like R. maximum and their associated soil microbiota. These findings have direct relevance to the potential for successful poleward migration or future assisted colonization efforts.

We demonstrate the application of seismic methods using in-mine infrastructure such as exploration tunnels to image platinum deposits and geologic structures using different acquisition configurations. In 2020, seismic experiments were conducted underground at the Maseve platinum mine in the Bushveld Complex of South Africa. These seismic experiments were part of the Advanced Orebody Knowledge project titled “Developing technologies that will be used to obtain information ahead of the mine face.” In these experiments, we recorded active and passive seismic data using surface nodal arrays and an in-mine seismic land streamer. We focus on analyzing only the in-mine active seismic portion of the survey. The tunnel seismic survey consisted of seven 2D profiles in exploration tunnels, located approximately 550 m below ground surface and a few meters above known platinum deposits. A careful data-processing approach was adopted to enhance high-quality reflections and suppress infrastructure-generated noise. Despite challenges presented by the in-mine noisy environment, we successfully imaged the platinum deposits with the aid of borehole data and geologic models. The results open opportunities to adapt surface-based geophysical instruments to address challenging in-mine environments for mineral exploration.

Abstract We investigate and analyze site specific systematics for the MAGIS-100 atomic interferometry experiment at Fermi National Accelerator Laboratory. As atom interferometers move out of the laboratory environment passive and active mitigation for noise sources must be implemented. To inform the research and development of the experiment design, we measure ambient temperature, humidity, and vibrations of the installation site. We find that temperature fluctuations will necessitate enclosures for critical subsystems and a temperature controlled laser room for the laser system. We also measure and analyze the vibration spectrum above and below ground for the installation site. The seismic vibration effect of gravity gradient noise is also modeled using input from a low-noise seismometer at multiple locations and a mitigation scheme is studied using a stochastic simulation and characterized by a suppression factor.

AbstractSoil temperature (ST) is an important property of soils and driver of below ground biogeochemical processes. Global change is responsible that besides variable meteorological conditions, climate-driven shifts in ST are observed throughout the world. In this study, we examined long-term records in ST by a trend decomposition procedure from eleven stations in western Germany starting from earliest in 1951 until 2018. Concomitantly to ST data from multiple depths (5, 10, 20, 50, and 100 cm), various meteorological variables were measured and included in the multivariate statistical analysis to explain spatiotemporal trends in soil warming. A significant positive increase in temperature was more pronounced for ST (1.76 ± 0.59 °C) compared with air temperature (AT; 1.35 ± 0.35 °C) among all study sites. Air temperature was the best explanatory variable to explain trends in soil warming by an average 0.29 ± 0.21 °C per decade and the trend peaked during the period from 1991–2000. Especially, the summer months (June to August) contributed most to the soil warming effect, whereby the increase in maximum ST (STmax) was nearby fivefold with 4.89 °C compared with an increase of minimum ST (STmin) of 1.02 °C. This widening between STmax and STmin fostered enhanced diurnal ST fluctuations at ten out of eleven stations. Subsoil warming up to + 2.3 °C in 100-cm depth is critical in many ways for ecosystem behavior, e.g., by enhanced mineral weathering or organic carbon decomposition rates. Thus, spatiotemporal patterns of soil warming need to be evaluated by trend decomposition procedures under a changing climate. Graphical abstract

The study was conducted to find the net carbon stock in the Sal (Shorea robusta) forest in Bagdaila Chisapani community forest of Dang district, Nepal. The inventory was done by a stratified sampling technique with 0.5% sampling intensity taking into account the woody plants with ≥ 5cm DBH. A total of 49 sample plots of radius 8.94m and 5.64 were established to measure tree biomass and sapling biomass respectively in the forest. The mean above-ground carbon (AGC) was 160.4 t ha-1 and the mean below-ground carbon (BGC) was 24.1t ha-1. The mean total carbon stock in the study area was estimated to be 99.02 t ha-1; of which maximum carbon stock of 143.51 t ha-1 was found in block number 5. Sal was the major tree species in the CF. The biomass and carbon content in this forest is found quite low as compared to other studies in the Sal forest and other tropical forests which were due to the presence of tree stands of less diameter and height. This study would be helpful in the long-term management of forests, planning, and research purposes. The data from this study could also be taken as a reference document for the participation of community forests in carbon accounting under the REDD+ scheme in Nepal.