Chromatography Intro
Chromatography Intro
Chromatography Intro
CHROMATOGRAPHY
Chromatography basically involves the separation of mixtures due to differences in the distribution coefficient (equilibrium distribution) of sample components between 2 different phases which are immiscible. One of these phases is a mobile phase and the other is a stationary phase.
DUE DIFFERNCE IN DISTRIBUTION COEFFICIENT OF VARIOUS COMPONENT OF MIXTURE, THERE MOBILITY IN TWO PHASES DIFFER HENCE, SEPARATION MEASURE OF THIS MOBILITY IS CALLED RF VALUE I.E. RELATIVE FRACTION VALUE
Rf=
STATIONARY PHASE: IN SOME CASES SOLID SUPPORT IT SELF FORM THE STATIONARY PHASE , WHILE IN OTHERS , IT ABSORBS LIQUID PHASE WHICH IN TURN ACTS AS STATIONARY PHASE. IT IS SOLID OR LIQUID.
MOBILE PHASE: PHASE WHICH MOVE OVER OR THROUGH STATIONARY PHASE AND CARRIES SAMPLE ALONG WITH IT, THUS RESULTING IN THE SEPARATION OF ITS COMPONENT. IT IS EITHER LIQUID OR GAS.
Kinds of Chromatography
1. Liquid-Liquid Column Chromatography
TLC, HPTLC
Elution
Eluent Chromatogram Chamber Saturation Detection Affinity
Partition Phenomena
This form of chromatography is based on a thin film formed on the surface of a
solid support by a liquid stationary phase. Solute equilibriates between the mobile phase and the stationary liquid. Examples- PLC, HPLC, GC, CC