The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.
The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.
The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.
The document provides instructions for a bioinformatics lab experiment involving multiple sequence alignment and molecular phylogeny. The objective is to understand and perform multiple sequence alignment using CLUSTAL W and phylogenetic analysis using PHYLIP. The experiment involves logging into the EMBL/EBI website, selecting CLUSTAL Omega, choosing the molecule type, appending sequences, running the default parameters, and pasting the alignment results. Multiple sequence alignment is used to find evolutionary relationships and common ancestry between three or more biological sequences.
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LABORATORY MANUAL
Exercise/Experiment Number: Lab Code / Lab : BTY412 Bioinformatics Lab Course / Branch : IV B.Tech. Biotechnology Title of the exercise/experiment : Multiple sequence alignment (CLUSTAL W) and Molecular phylogeny ______________________________________________________________
STEP 1: INTRODUCTION OBJECTIVE OF THE EXERCISE/EXPERIMENT To understand and perform the multiple sequence alignment and phylogenetic analysis using CLUSTAL W and PHYLIP, respectively.
STEP 2: ACQUISITION INTRODUCTION A multiple sequence alignment (MSA) is a sequence alignment of three or more biological sequences (protein, DNA, or RNA). From the query sequences we can find the evolutionary relationship by which they share a lineage and the descended from a common ancestor. Because three or more sequences of biologically relevant length can be difficult and are almost always time-consuming to align by hand, computational algorithms are used to produce and analyze the alignments. MSAs require more sophisticated methodologies than pairwise alignment because they are more computationally complex. Procedure: Step 1: Login to EMBL/ EBI website and select CLUSTAL Omega option under sequence analysis
Step 2: Select the type of the molecule to be clustered
Step 3: Select and append the sequences in the appropriate box
Step 4: Run the default parameters and submit. Paste the screenshot of the alignment produced
Result: The Clustal program was used to align related sequences
