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    DNA Extraction Practical

    Uploaded by

    Sharizah Bte Md Amin
    Students are encouraged to bring fruit of their choice to extract DNA from for a classroom activity. The fruit should be easy to mash by hand. The document provides instructions for extracting DNA from fruit by mashing the fruit in a ziplock bag, filtering out solids, and precipitating the DNA from solution using ethanol.

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    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd

    DNA Extraction Practical

    Uploaded by

    Sharizah Bte Md Amin
    667 views8 pages
    Students are encouraged to bring fruit of their choice to extract DNA from for a classroom activity. The fruit should be easy to mash by hand. The document provides instructions for extracting DNA from fruit by mashing the fruit in a ziplock bag, filtering out solids, and precipitating the DNA from solution using ethanol.

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    Students are encouraged to bring fruit of their choice to extract DNA from for a classroom activity. The fruit should be easy to mash by hand. The document provides instructions for extracting DNA from fruit by mashing the fruit in a ziplock bag, filtering out solids, and precipitating the DNA from solution using ethanol.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    667 views8 pages

    DNA Extraction Practical

    Uploaded by

    Sharizah Bte Md Amin
    Students are encouraged to bring fruit of their choice to extract DNA from for a classroom activity. The fruit should be easy to mash by hand. The document provides instructions for extracting DNA from fruit by mashing the fruit in a ziplock bag, filtering out solids, and precipitating the DNA from solution using ethanol.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 8

    Encourage your students to

    bring their own choice of fruit


    for the DNA extraction
    Reminder: Bring fruits that are easy to
    mash with their bare hands and
    compare the amounts of DNA extracted.

    HAPPY TRYING!
    DNA Extraction
    Which is the most important part of a
    cell?
    A place which keeps all the important
    documents - housed the genetic
    material called DNA

    Contain instructions to make proteins.

    Instructions can be communicated to


    cytoplasm through chemical
    messengers (e.g. letters / email /
    photocopy documents)

    Nucleus
    Play 2-parts video entitled
    DNA Extraction Intro (PART 1)
    DNA Extraction Home Experiment
    (PART 2)
    Too small to be seen?
    The DNA in each cell is very long and

    is packed into the small space within each


    nucleus as chromosomes.

    Although DNA is an incredibly small


    molecule, in large quantities, it can be
    seen.
    Steps in DNA extraction
    1. The cells must be lysed (breaking the
    physical membranes that enclosed them) to
    release the nucleus using extraction buffer.

    2. Meat tenderizer is used to partly degrade


    soluble proteins from DNA. (optional)

    3. DNA must be precipitated in ice cold


    ethanol.
    Procedure
    Every student will receive a ziplock bag.

    Add the fruit, sufficient water, ½ a teaspoon of salt and 2


    teaspoonful of detergent.

    Mash the contents of the ziplock bag till fine. Apply only sufficient
    force to do this so as not to break the ziplock bag.

    Let the contents of ziplock bag settle for 5 mins. Pour out the top
    most portion of the contents into the clear vial provided. (This is a
    simplified filtration process to separate the chunks of tissues
    from the soluble DNA and proteins.)

    Tilt the vial to 45º and very slowly add the 10 ml of ice cold 95%
    ethanol.
    What does DNA look like?

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