Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 19 views

    Dna Extraction Jariel

    Uploaded by

    Jariel Atillo
    This document describes an experiment to extract and compare DNA from bananas and kiwis. The experiment involved mashing banana and kiwi samples of similar weight, adding an extraction solution, and filtering the mixtures. Alcohol was added and DNA was observed to rise to the top layer. Trial results showed variable amounts of extracted DNA between fruits. The conclusion is that banana generally had a higher DNA yield than kiwi. Controlling variables like equal ingredient amounts could improve the experiment.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Dna Extraction Jariel

    Uploaded by

    Jariel Atillo
    19 views6 pages
    This document describes an experiment to extract and compare DNA from bananas and kiwis. The experiment involved mashing banana and kiwi samples of similar weight, adding an extraction solution, and filtering the mixtures. Alcohol was added and DNA was observed to rise to the top layer. Trial results showed variable amounts of extracted DNA between fruits. The conclusion is that banana generally had a higher DNA yield than kiwi. Controlling variables like equal ingredient amounts could improve the experiment.

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document describes an experiment to extract and compare DNA from bananas and kiwis. The experiment involved mashing banana and kiwi samples of similar weight, adding an extraction solution, and filtering the mixtures. Alcohol was added and DNA was observed to rise to the top layer. Trial results showed variable amounts of extracted DNA between fruits. The conclusion is that banana generally had a higher DNA yield than kiwi. Controlling variables like equal ingredient amounts could improve the experiment.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    19 views6 pages

    Dna Extraction Jariel

    Uploaded by

    Jariel Atillo
    This document describes an experiment to extract and compare DNA from bananas and kiwis. The experiment involved mashing banana and kiwi samples of similar weight, adding an extraction solution, and filtering the mixtures. Alcohol was added and DNA was observed to rise to the top layer. Trial results showed variable amounts of extracted DNA between fruits. The conclusion is that banana generally had a higher DNA yield than kiwi. Controlling variables like equal ingredient amounts could improve the experiment.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 6

    1

    DNA EXTRACTION

    Jariel D. Atillo

    STEM 04-General Biology, ACT Bulacao—Cebu

    STEM-11 Innovativeness

    Ms. Patrice Duane E. Vergara

    December 01,2022
    2

    I. INTRODUCTION

    In molecular biology, DNA replication is the biological process of producing two identical replicas

    of DNA from one original DNA molecule. DNA replication occurs in all living organisms acting as

    the most essential part for biological inheritance. This is essential for cell division during growth

    and repair of damaged tissues, while it also ensures that each of the new cells receives its own copy

    of the DNA. The cell possesses the distinctive property of division, which makes replication of

    DNA essential.

    A gene means manufacturing its corresponding protein, and this multilayered process has

    two major steps. In the first step, the information in DNA is transferred to a messenger RNA

    (mRNA) molecule by way of a process called transcription. During transcription, the DNA of a

    gene serves as a template for complementary base-pairing, and an enzyme called RNA polymerase

    II catalyzes the formation of a pre-mRNA molecule, which is then processed to form mature

    mRNA. The resulting mRNA is a single-stranded copy of the gene, which next must be translated

    into a protein molecule.

    Hypothesis

    •The DNA count of banana and kiwi could be the same or not.

    •The appearance of DNA in the banana is the same with kiwi.

    II. METHODS

    Step 1: Mash up the fruit of your choice in a bowl. The amount of fruit placed in the bag should be the
    3

    same weight to compare the DNA yields from different fruits. Below is the amount of fruit that we

    used had

    similar weight:

    - Banana = 1/3 of banana

    - Kiwi = ½ of kiwi

    Step 2: Add 3 tablespoons of extraction solution (Extraction Solution = 1 cup of water, 1 tablespoon of

    salt, 1 tablespoon of dish detergent)

    Step 3: Place strainer or coffee filter over a small beaker (paper cups can be used in place of a beaker).

    Step 4: : Pour the fruit mixture over the strainer and into a small beaker.

    Step 5: Add the same volume of chilled Alcohol to the small beaker. Be sure to pour alcohol down the

    inside of the beaker so the liquids don’t mix.

    Step 6: Allow the beaker to sit for 3 minutes and observe what happens to the alcohol layer. Time

    allows DNA to rise as an insoluble layer. During the 3 minutes, students can observe the DNA

    move to the top layer of the mixture.

    Step 7: Use tweezers to pull out the white thread-like DNA from the solution. You may not be able to

    pull out DNA in some fruit mixtures. You will see a white thread-like cloud appear at the top layer

    of the mixture. This is the fruit's DNA.


    4
    III. RESULTS

    BANANA
    TRIAL 1 TRIAL 2 TRIAL 3

    KIWI

    TRIAL 1 TRIAL 2 TRIAL 3

    IV. Discussion and conclusion


    The experiment involves the grinding and mashing of the fruit. This is to breakdown the

    fruit's cells thereby enlarging the surface area of the DNA. My experiment included many

    different part, therefore i needed to control my variables to make sure they do not affect the
    5

    amount of DNA I would extract from each fruit as the slices of fruit i cut. However we had not

    ensure that the amount of cold isopropyl we put in the experiment was equal to what was needed

    as well as the dishwashing, water, and the amount of salt. Some problems we encountered with

    the experiment was that the first trial of our banana. We mashed the banana first before we

    started the experiment. Due to the long process of lowering the temperature of the alcohol that

    almost reached the freezing point and the high exposure of open air that affected the appearance

    of the banana, it turned brown. It can be concluded that the amount of DNA between Kiwi and

    the Banana fruit can be vary. The DNA was extracted due to the dishwashing liquid bursting

    open the cells of the fruit, releasing the DNA that was inside and the salt helped ensure that the

    proteins in the cell are not separated from the rest of the solution of the DNA. The isopropyl

    alcohol allowed us to see due to it being insoluble after it was filtered with a cloth and strainer.

    My hypothesis proved to be correct that the DNA count of the Banana and Kiwi could be the

    same or not as seen at the picture within the Result section of the paper, additionally our second

    hypothesis is not correct. also to measure all the ingredients briefly. In our observation during the

    experiment, banana is the most dominant DNA count in which the appearance is yellowish color.

    V. REFERENCE

    Kiwifruit and Banana DNA Extraction.docx - Kiwifruit and Banana DNA Extraction

    Name& I'd: Shafa Salahudin Al Agusi & 21034970 aim: To: Course hero. DNA

    Extraction from Kiwi Fruit and Banana.docx - DNA Extraction from Kiwi Fruit and Banana

    Name & ID: Shafa Salahudin Al Agusi & 21034970 Aim: To | Course Hero. (n.d.).
    6

    Retrieved November 28, 2022, from https://www.coursehero.com/file/129801589/DNA-

    Extraction-from-Kiwi-Fruit-and-Bananadocx/ Zhou, C., & Ling, X. (2011, May 23). DNA

    binding and DNA extraction: Methods, applications, and limitations. Amazon. Retrieved

    November 28, 2022,

    from https://www.amazon.com/DNA-BindingExtraction-Modifications-Recombination/dp/1

    https://www.studocu.com/my/document/sunway-university/monash-university-foundation-year-

    614709580

    You might also like