FASTA Results

SUBMISSION PARAMETERS
Title Sequence Database uniprot
Sequence length 355 Sequence type p
Program fasta Version 35.04 Feb. 20, 2010
Expectation upper value 10.0 Matrix BL50
Sequence range 1- Number of scores 50
Number of alignments 50 Word size 2
Open gap penalty -10 Gap extension penalty -2
Histogram false  

Summary Table MView VisualFasta XML Printable SUBMIT ANOTHER JOB

FASTA searches a protein or DNA sequence data bank

version 35.04 Feb. 20, 2010
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448

Query: @
1>>>Sequence - 355 aa
Library: UniProt 4094566461 residues in 12649042 sequences

4094566461 residues in 12649042 sequences

Statistics: Expectation_n fit: rho(ln(x))= 6.8923+/-0.000233; mu= -6.1231+/- 0.013
mean_var=150.6391+/-27.482, 0's: 24 Z-trim: 1578 B-trim: 0 in 0/66
Lambda= 0.104497
statistics sampled from 60000 to 12626953 sequences
Algorithm: FASTA (3.5 Sept 2006) [optimized]
Parameters: BL50 matrix (15:-5) ktup: 2
join: 43, opt: 31, open/ext: -10/-2, width: 16
Scan time: 266.410

The best scores are: opt bits E(12649042)

UNIPROT:KRA51_HUMAN Q6L8H4 Keratin-associated prot ( 278) 715 120.3 7.7e-25
UNIPROT:A7MAW9_MOUSE A7MAW9 Putative uncharacteriz ( 238) 663 112.4 1.6e-22
UNIPROT:B9ZVX9_HUMAN B9ZVX9 Putative uncharacteriz ( 288) 621 106.1 1.5e-20
UNIPROT:KRA54_HUMAN Q6L8H1 Keratin-associated prot ( 288) 610 104.5 4.6e-20
UNIPROT:C9J1K0_HUMAN C9J1K0 Putative uncharacteriz ( 288) 601 103.1 1.2e-19
UNIPROT:C5XDQ6_SORBI C5XDQ6 Putative uncharacteriz ( 400) 603 103.6 1.2e-19
UNIPROT:U88_HHV6U Q69566 Uncharacterized protein U ( 413) 603 103.6 1.2e-19
UNIPROT:D4A2S7_RAT D4A2S7 Putative uncharacterized ( 287) 595 102.2 2.2e-19
UNIPROT:KRA55_MOUSE Q2TA51 Keratin-associated prot ( 241) 590 101.4 3.3e-19
UNIPROT:D3ZQU5_RAT D3ZQU5 Putative uncharacterized ( 197) 588 101.0 3.5e-19
UNIPROT:D3ZEA5_RAT D3ZEA5 Putative uncharacterized ( 236) 584 100.5 6e-19
UNIPROT:A6QQG8_BOVIN A6QQG8 KRTAP9-2 protein OS=Bo ( 296) 582 100.3 8.7e-19
UNIPROT:A2A5X3_MOUSE A2A5X3 Putative novel member ( 358) 583 100.5 9e-19
UNIPROT:B8Y995_ORYSI B8Y995 Keratin associated pro ( 426) 584 100.7 9.2e-19
UNIPROT:Q67UU9_ORYSJ Q67UU9 DENSE PANICLE 1 OS=Ory ( 426) 583 100.6 1e-18
UNIPROT:D3YZ38_MOUSE D3YZ38 Putative uncharacteriz ( 243) 577 99.4 1.3e-18
UNIPROT:A8MUN0_HUMAN A8MUN0 Putative uncharacteriz ( 228) 571 98.5 2.3e-18
UNIPROT:A6NFW2_HUMAN A6NFW2 Putative uncharacteriz ( 219) 570 98.3 2.5e-18
UNIPROT:B8BCF5_ORYSI B8BCF5 Putative uncharacteriz ( 426) 572 98.9 3.2e-18
UNIPROT:KRA52_MOUSE Q9D5Z7 Keratin-associated prot ( 189) 566 97.7 3.4e-18
UNIPROT:D3ZNF8_RAT D3ZNF8 Putative uncharacterized ( 193) 564 97.4 4.2e-18
UNIPROT:C5YLU2_SORBI C5YLU2 Putative uncharacteriz ( 290) 563 97.4 6.3e-18
UNIPROT:D4A3Q1_RAT D4A3Q1 Putative uncharacterized ( 305) 552 95.8 2e-17
UNIPROT:D3ZAY6_RAT D3ZAY6 Putative uncharacterized ( 224) 544 94.4 3.8e-17
>>UNIPROT:KRA51_HUMAN Q6L8H4 Keratin-associated protein (278 aa)
initn: 586 init1: 328 opt: 715 Z-score: 605.9 bits: 120.3 E(): 7.7e-25
Smith-Waterman score: 718; 36.1% identity (61.3% similar) in 269 aa overlap (104-
355:4-250)

80 90 100 110 120 130

Sequen CCTCATTCTTCGCTCTCATGTCCCTCCTTGCACTGGCGATGTCCGTGCCTC-TCCAGAAA
:.:.::::.. ::.:: : . :.:
UNIPRO MGCCGCSGGCGSSCGGCGSGCGGCGSGCGG---
10 20 30

140 150 160 170

Sequen CGAGATGTCTTCGTCCC---CCCGGTCC---TCGATCCTCA---CCAGGG--GAC--CGT
::.: : :. :: ::: .:: :: .: .:. : ..:: :.: ::

Multalin version 5.4.1

Copyright I.N.R.A. France 1989, 1991, 1994, 1996
Published research using this software should cite
Multiple sequence alignment with hierarchical clustering
F. CORPET, 1988, Nucl. Acids Res., 16 (22), 10881-10890
Symbol comparison table: blosum62
Gap weight: 12
Gap length weight: 2
Consensus levels: high=90% low=50%
Consensus symbols:
! is anyone of IV
$ is anyone of LM
% is anyone of FY
# is anyone of NDQEBZ

MSF: 689 Check: 0 ..

Name: pr1 Len: 689 Check: 7301 Weight: 1.34
Name: pr2 Len: 689 Check: 2948 Weight: 1.10
Name: pr3 Len: 689 Check: 7728 Weight: 0.56
Name: pr5 Len: 689 Check: 5132 Weight: 0.56
Name: pr4 Len: 689 Check: 4800 Weight: 1.44
Name: Consensus Len: 689 Check: 9844 Weight: 0.00

//
MAFFT-L-INS-i Result

CLUSTAL format alignment by MAFFT (v6.832b)

pr1 acctctctctcgaacacctccacatcctttacttactaccctcccaagatgatgttcacg
pr4 ------------------------------------------------------------
pr2 ttccgtaggtgaacctgcggaaggatcattatcgaaaccc-------gagggtgcagagg
pr3 ------------------ggaaggatcattatcgaaa-cc-------gagggtgcagagg
pr5 ---------------------------------------------------------atg

pr1 aagtctctcatgtcctcattcttcgctctcatgtccctccttgcactggcgatgtccgtg
pr4 atgcgcttcactatcgctctcttcgccgctcttccccttctcgtcgccgcgagcgccgt-
pr2 gggatcggagagatccgttcctccgaagcccttcgtttactctttgacac----------
pr3 gggatcggagagatccgttcctccgaagcccttcgtttactctttgacac----------
pr5 aagttcgccgccgtcgtcgtcttagctgctgctgccgctgccgtctctgctgagaccaat
* * ** * *

pr1 cctctccagaaacgagatgtcttcgtccccccggtcctcgatcctcaccaggggacc---
pr4 ------------------------------------------------------------
pr2 ------------------------------------------------------------
pr3 ------------------------------------------------------------
pr5 gctcagcgtatggctcgtggcctccctcccaaggcccccatccgtcgtcacggtactccc
pr1 -----------------gtctggtacgttgggca--------------------------
pr4 -----------------acctcgtgcgccgggtggtcagaagtgcc------gtgcggag
pr2 -----------------acctcacgtgcaccccattcgcaagggcc--------------
pr3 -----------------acctcacgtgcaccccattcgcaagggcc--------------
pr5 gctgacacggagaaaagatcccatccttcctctactggcggtggccagtgcaacactgga
*

pr1 ---------------------------acaagcagaagtcgtctgggacacgagtaacgc
pr4 tcggtccagtgctgcaacacgacttacagccacaaggactactcgcacgtcgaatacctc
pr2 --------------------cttcgggacccgcgcgtttcatctccgactcgcatgtcta
pr3 --------------------cttcgggacccgcgcgtttcatctccgactcgcatgtcta
pr5 ccaattcagtgctgcaacaccgtcgctacctctggttctcaatcgggcgttgacgagctt
* * *

pr1 ccctgcacaaatcaca-------------------------------aattcagagggac
pr4 cagggtctcggtatcagccctgaggtcctggccaaccccgacgctagcagttacgctgac
pr2 cagaatgtcgatagca----------tttgatatatataaaaaatacaactttcagcaac
pr3 cagaatgtcgatagca----------tttgatatatataaaaaatacaactttcagcaac
pr5 ctcaccctccttggcc---------tctcggtccccgtcggcacgcaagtcggtgctagc
* * * *

pr1 agattta----tctcgtcgtaaacaacctcatcgatttcgactacttgttggcaaatgat
pr4 tgcaccccattgtccgtcgtgggcggccacggctgtcaggcgtcgtctactgccgtctgt
pr2 ggatctc------------ttggctctcgcatcgatgaagaac--------gcagcgaat
pr3 ggatctc------------ttggctctcgcatcgatgaagaac--------gcagcgaat
pr5 tgctctccaatttctgctgtcggcactggcagcggtgcacaatgctcgggccagactgtt
* * * * * * *

pr1 ttcaatattctcgatgggagtgtgatggtca---cagtaccggacg--------------
pr4 tgcgagaacaactaccagcgcggcgctgt-----caacatgggatgcgtg----------
pr2 cgcgataagtaatgtgaattgcagattttccgtgaatcatcgaatctttgaacgcacc--
pr3 cgcgataagtaatgtgaattgcagattttccgtgaatcatcgaatctttgaacgcacctt
pr5 tgctgcgaacaaaatgagt--ggaatggtttggtcaacattggttgtatg----------
* * * * *

pr1 ------------------------------------------------------------
pr4 ------------------------------------------------------------
pr2 ------------------------------------------------------------
pr3 gtgctccttggtattccgaggagcatgcctgtttgagtgtcatcgaaatctcaaaccaag
pr5 ------------------------------------------------------------

pr1 ------------------------------------------------------------
pr4 ------cccattagcatggctgcttaa---------------------------------
pr2 ------------------------------------------------------------
pr3 cttttcttgacttcggtcgaaggctcgggtttggaccgttgggagtctgcgggcgacgca
pr5 ------cccatcaacctcaacgcttagttccccaatccatcgacgtgtccagcacgttcc

pr1 ------------------------------------------------------------
pr4 ------------------------------------------------------------
pr2 ------------------------------------------------------------
pr3 tgtcgtcggctctcctgaaatgcattagcggtgggcatgcaagtcttgcttggcaccagc
pr5 gctccttattcttgttgacacgacctcacaggtcatagacgcttcacacttaatactacc

pr1 ------------------------------------------------------------
pr4 ------------------------------------------------------------
pr2 ------------------------------------------------------------
pr3 ctctcccggcgtcatagtgatcgtcgcgggctgtccagctgcaagggacatgtcccatgc
pr5 tcgcatggtatcttttgttttcaatacccttcgtccatttttaggttgtttttgttgggt

pr1 ------------------------------------------------------------
pr4 ------------------------------------------------------------
pr2 ------------------------------------------------------------
pr3 ttctccaactttgcgagccctctcctgggctctgcgttcgaaggcttgacctcaaa----
pr5 gtgtccgtgcaatgcgttgacgtcttggttaccgtccgtaatgcttggcactggtagatt

pr1 ---------------------
pr4 ---------------------
pr2 ---------------------
pr3 -----------------tcag
pr5 gcaatggttaaaaggttcact

CLUSTAL 2.0.12 multiple sequence alignment

pr2 TTCCGTAGGTGAACCTGCGGAAGGATCATTATCGAAACCCGAGGGTGCAGAGGGGGATCG 60
pr3 ------------------GGAAGGATCATTATCGAAACC-GAGGGTGCAGAGGGGGATCG 41
pr1 ------------ACCTCTCTCTCGAACACCTCCACATCCTTTACTTACTACCCTCCCAAG 48
pr5 ---------ATGAAGTTCGCCGCCGTCGTCGTCTTAGCTGCTGCTGCCGCTGCCGTCTCT 51
pr4 ------------------------------------------------------------
pr2 GAGAGATCCGTT---CCTCCGAAGCCCTTCGTTTACTCTTTGACACACCTCACGTGCACC 117
pr3 GAGAGATCCGTT---CCTCCGAAGCCCTTCGTTTACTCTTTGACACACCTCACGTGCACC 98
pr1 ATGATGTTCACGAAGTCTCTCATGTCCTCATTCTTCGCTCTCATGTCCCTC-CTTGCACT 107
pr5 GCTGAGACCAATGCTCAGCGTATGGCTCGTGGCCTCCCTCCCAAGGCCCCCATCCGTCGT 111
pr4 ------------------------------ATGCGCTTCACTATCGCTCTCTTCGCCGCT 30
* * * *

pr2 CCATTCGCAAGGGCCCTTCGGGACCCGCGCGTTTCATCTCCGACTCGCA-----TGTCTA 172

pr3 CCATTCGCAAGGGCCCTTCGGGACCCGCGCGTTTCATCTCCGACTCGCA-----TGTCTA 153
pr1 GGCGATGTCCGTGCCTCTCCAGAAACGAG-ATGTCTTCGTCCCCCCGGT-----CCTCGA 161
pr5 CACGGTACTCCCGCTGACACGGAGAAAAG-ATCCCATCCTTCCTCTACTGGCGGTGGCCA 170
pr4 CTTCCCCTTCTCGTCGCCGC-GAGCGCCGTACCTCGTGCGCCGGGTGGTCAGAAGTGCCG 89
* ** * * * *

pr2 -CAGAATGTCGATAGCATTTGATAT-AT-----ATAAAAAATACAACTT-TCAGCAACGG 224

pr3 -CAGAATGTCGATAGCATTTGATAT-AT-----ATAAAAAATACAACTT-TCAGCAACGG 205
pr1 TCCTCACCAGGGGACCGTCTGGTAC-GTTGG--GCAACAAGCAGAAGTCGTCTGGGACAC 218
pr5 GTGCAACACTGGACCAATTCAGTGCTGCAAC--ACCGTCGCTACCTCTGGTTCTCAATCG 228
pr4 -TGCGGAGTCGGTCCAGTGCTGCAACACGACTTACAGCCACAAGGACTACTCGCACGTCG 148
* * * * *

pr2 ATC----TCTTGGCTCTCGCATCGATGAAGAACGCAGCGAATC-GCGATAAGTAATGTGA 279

pr3 ATC----TCTTGGCTCTCGCATCGATGAAGAACGCAGCGAATC-GCGATAAGTAATGTGA 260
pr1 GAG----TAACG-CCCCTGCACAAATCACAAATTCAGAGGGAC-A-GATTTATCTCGTCG 271
pr5 GGCG--TTGACGAGCTTCTCACCCTCCTTGGCCTCTCGGTCCCCGTCGGCACGCAAGTCG 286
pr4 AATACCTCCAGGGTCTCGGTATCAGCCCTGAGGTCCTGGCCAACCCCGACGCTAGCAGTT 208
* * * *

pr2 ATTGCAGATTTTCCGTGAATCATCGAATCTTTGAACGCACC------------------- 320

pr3 ATTGCAGATTTTCCGTGAATCATCGAATCTTTGAACGCACCTTGTGCTCCTTGGTATTCC 320
pr1 TAAACAA---CCTCATCGATT-TCGACTACTTGTTGGCAAATGAT-TTCAATATTCTCGA 326
pr5 GTGCTAGCTGCTCTCCAATTTCTGCTGTCGGCACTGGCAGC-GGTGCACAATGCTCGGGC 345
pr4 ACGCTGACTGCACCCCATTGTCCGTCGTGGGCGGCCACGGCTGTCAGGCGTCGTCTACT- 267
* *

pr2 ------------------------------------------------------------
pr3 GAGGA-GCATGCCTGTTTGAGTGTCATCGAAATCTCAAACCAAGCTTTTCTTGACTTCGG 379
pr1 TGGGA-GTGTGATGGTCACAGTACCGGACG------------------------------ 355
pr5 CAGACTGTTTGCTGCGAACAAAATGAGTGGAATGGTTTGGTCAACATTGGTTGTATGCCC 405
pr4 ---GCCGTCTGTTGCGAGAACAACTACCAGCGCGGCGCTGTCAACATGGGATGCGTGCCC 324

pr2 ------------------------------------------------------------
pr3 TCGAAGGCTCGGGTTTGG-------ACCGTTGGGAGTCTGCGGGCGACGCATGTCGTCGG 432
pr1 ------------------------------------------------------------
pr5 ATCAACCTCAACGCTTAGTTCCCCAATCCATCGACGTGTCCAGCACGTTCCGCTCCTTAT 465
pr4 ATTAGCATGGCTGCTTAA------------------------------------------ 342

pr2 ------------------------------------------------------------
pr3 CTCTCCTGAAATGCATTAGCGGTGGGCATGCAAGTCTTGCTTGGCACCAGCCTCTCCCGG 492
pr1 ------------------------------------------------------------
pr5 TCTTGTTGACACGACCTCACAGGTCATAGACGCTTCACACTTAATACTACCTCGCATGGT 525
pr4 ------------------------------------------------------------

pr2 ------------------------------------------------------------
pr3 CGTCATAGTGATCGTCGCGGGCTGTCCAGCTGCAAGGGACATGTCCCATGCTTCTCCAAC 552
pr1 ------------------------------------------------------------
pr5 ATCTTTTGTTTTCAATACCCTTCGTCCATTTTTAGGTTGTTTTTGTTGGGTGTGTCCGTG 585
pr4 ------------------------------------------------------------

pr2 ------------------------------------------------------------
pr3 TTTGCGAGCCCTCTCCTGGGCTCTGCGTTCGAAGGCTTGACCTCAAATCAG--------- 603
pr1 ------------------------------------------------------------
pr5 CAATGCGTTGACGTCTTGGTTACCGTCCGTAATGCTTGGCACTGGTAGATTGCAATGGTT 645
pr4 ------------------------------------------------------------

pr2 ------------
pr3 ------------
pr1 ------------
pr5 AAAAGGTTCACT 657
pr4 ------------