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    ICSI

    Uploaded by

    le sage
    ICSI is a form of IVF that involves directly injecting a single sperm into a human egg to facilitate fertilization. It is commonly used to treat male factor infertility issues like oligozoospermia, azoospermia, or sperm motility problems. ICSI can also be used when standard IVF has failed repeatedly or is the preferred method at some fertility clinics. Genetic testing of both partners is important for cases of severe male factor infertility due to risks of passing on genetic infertility. ICSI has comparable success rates to standard IVF and has largely replaced other microinjection techniques. Multiple pregnancies are a risk if more than one embryo is transferred.

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    ICSI

    Uploaded by

    le sage
    57 views7 pages
    ICSI is a form of IVF that involves directly injecting a single sperm into a human egg to facilitate fertilization. It is commonly used to treat male factor infertility issues like oligozoospermia, azoospermia, or sperm motility problems. ICSI can also be used when standard IVF has failed repeatedly or is the preferred method at some fertility clinics. Genetic testing of both partners is important for cases of severe male factor infertility due to risks of passing on genetic infertility. ICSI has comparable success rates to standard IVF and has largely replaced other microinjection techniques. Multiple pregnancies are a risk if more than one embryo is transferred.

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    ICSI is a form of IVF that involves directly injecting a single sperm into a human egg to facilitate fertilization. It is commonly used to treat male factor infertility issues like oligozoospermia, azoospermia, or sperm motility problems. ICSI can also be used when standard IVF has failed repeatedly or is the preferred method at some fertility clinics. Genetic testing of both partners is important for cases of severe male factor infertility due to risks of passing on genetic infertility. ICSI has comparable success rates to standard IVF and has largely replaced other microinjection techniques. Multiple pregnancies are a risk if more than one embryo is transferred.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    57 views7 pages

    ICSI

    Uploaded by

    le sage
    ICSI is a form of IVF that involves directly injecting a single sperm into a human egg to facilitate fertilization. It is commonly used to treat male factor infertility issues like oligozoospermia, azoospermia, or sperm motility problems. ICSI can also be used when standard IVF has failed repeatedly or is the preferred method at some fertility clinics. Genetic testing of both partners is important for cases of severe male factor infertility due to risks of passing on genetic infertility. ICSI has comparable success rates to standard IVF and has largely replaced other microinjection techniques. Multiple pregnancies are a risk if more than one embryo is transferred.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 7

    ICSI

    Indications and results


    Intracytoplasmic Sperm Injection

     Is a form of IVF (‘IVF with ICSI’)


     Male factor infertility
    • Oligozoospermia
    • Azoospermia
    • Sperm motility problems
    – Epididymal and testicular sperm (non-mature and
    non-motile) PESA, MESA, MESE
    – Cogenital BAVD (ciliary defect)
     Repeated failed standard IVF
     Changing indications : some labs now only offer ICSI. Most still
    offer IVF or IVF with ICSI
    Genetics
     Karyotype analysis is essential in
    – severe oligozoospermia (6-7%)
    – azoospermia (15%)
    • mutations in the long arm of the y chromosome
    Yq in the AZFc (DAZ gene). Offspring if male
    will also be infertile.
    – CBAVD : check for cystic fibrosis gene in patient
    plus partner
    ICSI
     Oocyte collection. Stimulate superovulation with human
    menopausal gonadotrophin (hMG) or pure recombinant FSH,
    then hCG

     Ovaries enlarge to tennis ball size. Oocytes removed by needle


    aspiration through posterior fornix with TVUSS guidance

     Remove cumulus cells. Inject a paralysed sperm into the


    cytoplasm.

     Fertilization rate is 60-70% with < 10 % ova damaged during


    ICSI
    IVF / ICSI
    ICSI
    Partial
    Zona
    Diossection
    PZD

    Standard IVF

    Subzonal
    Injection
    SUZI
    Results
     Sperm can be retrieved in 100% of men with obstructive
    azoospermia and 50% of men with non obstructive azoospermia.
    Even in sertoli-cell only etc : pockets with PESA/MESA and MESE

     In year 2000 in the UK


    – 12051 cycles of IVF : 2861 live births = 23.7 % THBR
    – 9322 cycles of ICSI : 2246 live births = 24.1% THBR
    – < 10% oocytes were damaged during ICSI

     30-40% multiple pregnancy rate (2-3 zygotes used <35 yrs female)

     In 1998 in the UK
    – 7937 babies born with IVF / ICSI : congenital abnormality rate almost
    identical to general population 1.1 % / 1.6%
    ICSI MCQ
     Has a significant risk of multiple pregnancy because more than
    one sperm can be accidentally injected
     Has superceded partial zona dissection (PZD) and subzonal
    injection (SUZI)
     Is regulated by the Human Fertilization and Embryology
    Authority (HFEA) after passing of the Human Fertilization and
    Embryology Act in 1990
     Is indicated only in male factor infertility
     Has had a negative impact on the thorough treatment of
    obstructive azoospermia by Urologists

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