Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 106 views

    VTT Plant Biotech

    Uploaded by

    vttevent
    Plants are an important source of pharmaceutical compounds due to their chemical diversity and ability to produce compounds that are difficult to synthesize. Plant biotechnology provides sustainable methods for producing these high-value compounds through plant cell and tissue cultures, which allow controlled production without environmental impacts. VTT has extensive capabilities in plant biotechnology including establishing cell cultures, characterizing biosynthetic pathways, enhancing production through elicitation and genetic engineering, optimizing bioreactor production, and scaling up to industrial levels. As an example, VTT developed an Arctic cloudberry cell culture to produce compounds for use in cosmetics.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd

    VTT Plant Biotech

    Uploaded by

    vttevent
    106 views20 pages
    Plants are an important source of pharmaceutical compounds due to their chemical diversity and ability to produce compounds that are difficult to synthesize. Plant biotechnology provides sustainable methods for producing these high-value compounds through plant cell and tissue cultures, which allow controlled production without environmental impacts. VTT has extensive capabilities in plant biotechnology including establishing cell cultures, characterizing biosynthetic pathways, enhancing production through elicitation and genetic engineering, optimizing bioreactor production, and scaling up to industrial levels. As an example, VTT developed an Arctic cloudberry cell culture to produce compounds for use in cosmetics.

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Plants are an important source of pharmaceutical compounds due to their chemical diversity and ability to produce compounds that are difficult to synthesize. Plant biotechnology provides sustainable methods for producing these high-value compounds through plant cell and tissue cultures, which allow controlled production without environmental impacts. VTT has extensive capabilities in plant biotechnology including establishing cell cultures, characterizing biosynthetic pathways, enhancing production through elicitation and genetic engineering, optimizing bioreactor production, and scaling up to industrial levels. As an example, VTT developed an Arctic cloudberry cell culture to produce compounds for use in cosmetics.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    106 views20 pages

    VTT Plant Biotech

    Uploaded by

    vttevent
    Plants are an important source of pharmaceutical compounds due to their chemical diversity and ability to produce compounds that are difficult to synthesize. Plant biotechnology provides sustainable methods for producing these high-value compounds through plant cell and tissue cultures, which allow controlled production without environmental impacts. VTT has extensive capabilities in plant biotechnology including establishing cell cultures, characterizing biosynthetic pathways, enhancing production through elicitation and genetic engineering, optimizing bioreactor production, and scaling up to industrial levels. As an example, VTT developed an Arctic cloudberry cell culture to produce compounds for use in cosmetics.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 20

    VTT Plant biotechnology

    VTT Technical Research Centre of Finland

    03/09/2012

    Why plant biotechnology


    Plants are an important source of high-value compounds
    Excellent chemical diversity Chemical space different from chemically synthesized compounds

    Plants have versatile properties for e.g. pharmaceutical production


    Small molecules (Complex structures often with stereochemistry impossible to synthetize) Proteins (natural and recombinant)

    03/09/2012

    Plants are important sources of pharmaceuticals

    Opiumalkaloid: Morfine Analgesic

    Terpene: Artemisine Antimalarial

    Lignane: Podofyllotoxin derivative Anticancer

    Quinoline alkaloid: Quinine Antimalarial

    Terpene: Paclitaxel Anticancer

    Steroidglycoside: Digoxin Cardiac drug

    Today about a quarter of all pharmaceuticals on the market are of plant origin

    03/09/2012

    Why use plant biotechnology for the production of food and cosmetic raw materials

    Sustainability preservation of rare plants in nature Flexibility the whole plant biodiversity can be utilized Advantages of using cell cultures
    Produced in controlled sterile conditions in bioreactors High purity, no environmental pollutants High quality, good batch to batch repeatibility Scalable production process VTT has proprietary dry fractionation technology No extraction solvents used

    03/09/2012

    Definition of plant biotechnology

    Techniques to culture in vitro tissue fragments, cell aggregates and individual cells in synthetic growth medium in controlled, sterile conditions Methods by which biological processes can be controlled

    Rate of production enables economical industrial production

    03/09/2012

    Problems of traditional production methods


    Plants as producers (wild or cultivated)

    Low contents/growth Stage/organ specific accumulation Cultivation problems Over-collection of endangered species

    Chemical synthesis

    Complex structures, chemical synthesis not possible or economically unfeasible

    Biotechnological production in plant cell and tissue cultures

    03/09/2012

    Taxus brevifolia Pacific yew

    Paclitaxel
    Anti-cancer drug

    27 000 tons of Taxus brevifolia bark and harvesting of 12 000 trees would be needed to obtain 2.5 kg paclitaxel Currently pacitaxel is produced in plant cell cultures by Phyton

    VTT plant biotechnology platform


    Characterization of biosynthetic pathways to desired molecule

    03/09/2012

    Establishment of tissue or cell culture from a plant

    Functional genomics Gene discovery e.g. through deep sequencing Proteomics, metabolomics Data integration Functional analysis

    Enhancement of production by elicitation

    Enhancement of production by biotransformation

    Enhancement of production by genetic engineering

    Optimization of production conditions in bioreactor environment

    Scale-up to 1200 L in biomanufacturing pilot plant

    03/09/2012

    The biosynthetic pathway of morphine


    Tyrosine 5 steps S-Norcolaurine Berberine 4 steps ps l S-Reticuline era Sev ps 2 steps ste R-Reticuline 4 steps Naloxone Etorphine Buprenorphine Several steps Several steps Papaverine

    4 ste

    The enzymology of the entire morphine pathway is now largely elucidated after 25 years of research For most of the enzymes, genes have been cloned

    Noscapine

    Sanguinarine

    1 step Oripavine 1 step Morphinone

    Thebaine 3 steps Codeine 1 step Morphine

    Semisynthetic derivatives

    1 step

    Semisynthetic derivatives

    Ethylmorphine Diamorphine

    VTT plant biotechnology platform


    Characterization of biosynthetic pathways to desired molecule

    03/09/2012

    10

    Establishment of tissue or cell culture from a plant

    Functional genomics Gene discovery e.g. through deep sequencing Proteomics, metabolomics Data integration Functional analysis

    Enhancement of production by elicitation

    Enhancement of production by biotransformation

    Enhancement of production by genetic engineering

    Optimization of production conditions in bioreactor environment

    Scale-up to 1200 L in biomanufacturing pilot plant

    03/09/2012

    11

    VTT Metabolomics platform


    Samples Profiling experiments Automated extraction methods (NMR, (UP)LC/MS, GC/GC-ToF/MS) and analytical platforms
    Global screening (hydrophilic fraction) Global screening (lipid fraction) Eicosanoids and fatty acids Others

    Multivariate statistical analyses Data integration Bio-/chemo-informatics knowledge mining

    Biological insight

    Data processing Identification

    VTT plant biotechnology platform


    Characterization of biosynthetic pathways to desired molecule

    03/09/2012

    12

    Establishment of tissue or cell culture from a plant

    Functional genomics Gene discovery e.g. through deep sequencing Proteomics, metabolomics Data integration Functional analysis

    Enhancement of production by elicitation

    Enhancement of production by biotransformation

    Enhancement of production by genetic engineering

    Optimization of production conditions in bioreactor environment

    Scale-up to 1200 L in biomanufacturing pilot plant

    03/09/2012

    13

    Establishment of tissue or cell culture from a plant

    Plant / Berry Initiation of the culture

    Callus formation Cell line selection Suspension culture

    VTT plant biotechnology platform


    Characterization of biosynthetic pathways to desired molecule

    03/09/2012

    14

    Establishment of tissue or cell culture from a plant

    Functional genomics Gene discovery e.g. through deep sequencing Proteomics, metabolomics Data integration Functional analysis

    Enhancement of production by elicitation

    Enhancement of production by biotransformation

    Enhancement of production by genetic engineering

    Optimization of production conditions in bioreactor environment

    Scale-up to 1200 L in biomanufacturing pilot plant

    03/09/2012

    15

    Enhancement of production with different methods


    Elicitation
    Biotransformation

    Genetic engineering
    Stressing the cells with methyl jasmonate Increases production of a secondary metabolite. The difference can be visually seen. -methyl digoxine is easy to produce with biotransformation from a cheap precursor.
    N CH3
    H6H

    N H O H

    CH3
    H6H

    N H O

    CH3

    O OH

    HO

    O OH H

    H O

    O OH H

    Hyoscyamine

    6-Hydroxyhyoscyamine

    Scopolamine

    Overexperssion of H6H gene causes 100 fold increase in scopolamine production in transgenic cells.

    VTT plant biotechnology platform


    Characterization of biosynthetic pathways to desired molecule

    03/09/2012

    16

    Establishment of tissue or cell culture from a plant

    Functional genomics Gene discovery e.g. through deep sequencing Proteomics, metabolomics Data integration Functional analysis

    Enhancement of production by elicitation

    Enhancement of production by biotransformation

    Enhancement of production by genetic engineering

    Optimization of production conditions in bioreactor environment

    Scale-up to 1200 L in biomanufacturing pilot plant

    03/09/2012

    17

    Up-stream processing photobioreactor disposable wave bioreactors 43 stirred-tank bioreactors in 0.2 20 L scale fully equipped biomanufacturing pilot facility (30 300 1200 L) Down-stream processing drum filter, press filter continuous centrifuge chromatographic separation techniques micro and ultrafiltration equipment

    03/09/2012

    18

    VTT Biotechnology selected references


    Berry stem cells for cosmetics
    VTT Plant Biotechnology developed an Arctic cloudberry cell culture to be utilized for cosmetics raw material Product launched by Lumene Oy is the first commercial product in the world to utilize the cell culture of Arctic berries

    03/09/2012

    19

    Cloudberry stem cell culture for cosmetics


    Experimental data of berry bioactivites

    Suspension cell culture established from berry plant

    Production in small scale

    Bioactivity, cytotoxicity and formulation testing

    Production in 300 L bioreactor environment

    Biomass harvest and filtering

    Lyophilization and packing

    Raw material ready for a cosmetic product

    Photo Jouko Lehmuskallio

    03/09/2012

    20

    VTT - 70 years of

    technology for business and society

    You might also like