GENEBoy

Secuencia a estudiar: ATGTGCGACCATACCAAGCTGAAAATACTGCATCCCGTCTGATCTGCACAGTCAAGCAGCTTAGGGCCC AGTCAGTAGTGCGGTGGGGGACCATGCGCGAACATTGTGGTGTTGCACTTATGTGCGACCATACCAAG CTGAAAATACTGCATCCCGTCTGATCTGCACAGTCAAGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGG GGGACCATGCGCGAACATTGTGGTGTTGCACTTATGTGCGACCATACCAAGCTGAAAATACTGCATCCC GTCTGATCTGCACAGTCAAGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGGGGGACCATGCGCGAACAT TGTGGTGTTGCACTTATGTGCGACCATACCAAGCTGAAAATACTGCATCCCGTCTGATCTGCACAGTCA AGCAGCTTAGGGCCCAGTCAGTAGTGCGGTGGGGGACCATGCGCGAACATTGTGGTGTTGCACTT 1- Secuencia Reversa: TTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACTGACCCGGGATTCGACGAACT GACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTGTATTCACGTTGTGGTGTTAC AAGCGCGTACCAGGGGGTGGCGTGATGACTGACCCGGGATTCGACGAACTGACACGTCTAGTCTGCCC TACGTCATAAAAGTCGAACCATACCAGCGTGTATTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGG TGGCGTGATGACTGACCCGGGATTCGACGAACTGACACGTCTAGTCTGCCCTACGTCATAAAAGTCGA ACCATACCAGCGTGTATTCACGTTGTGGTGTTACAAGCGCGTACCAGGGGGTGGCGTGATGACTGACC CGGGATTCGACGAACTGACACGTCTAGTCTGCCCTACGTCATAAAAGTCGAACCATACCAGCGTGTA

2- Secuencia complementaria: TACACGCTGGTATGGTTCGACTTTTATGACGTAGGGCAGACTAGACGTGTCAGTTCGTCGAATCCCGGG TCAGTCATCACGCCACCCCCTGGTACGCGCTTGTAACACCACAACGTGAATACACGCTGGTATGGTTCG ACTTTTATGACGTAGGGCAGACTAGACGTGTCAGTTCGTCGAATCCCGGGTCAGTCATCACGCCACCCC CTGGTACGCGCTTGTAACACCACAACGTGAATACACGCTGGTATGGTTCGACTTTTATGACGTAGGGCA GACTAGACGTGTCAGTTCGTCGAATCCCGGGTCAGTCATCACGCCACCCCCTGGTACGCGCTTGTAACA CCACAACGTGAATACACGCTGGTATGGTTCGACTTTTATGACGTAGGGCAGACTAGACGTGTCAGTTCG TCGAATCCCGGGTCAGTCATCACGCCACCCCCTGGT ACGCGCTTGTAACACCACAACGTGAA

3- Transcrito: AUGUGCGACCAUACCAAGCUGAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGG GCCCAGUCAGUAGUGCGGUGGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACC AUACCAAGCUGAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAG UAGUGCGGUGGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACCAUACCAAGCU GAAAAUACUGCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAGUAGUGCGGU GGGGGACCAUGCGCGAACAUUGUGGUGUUGCACUUAUGUGCGACCAUACCAAGCUGAAAAUACU GCAUCCCGUCUGAUCUGCACAGUCAAGCAGCUUAGGGCCCAGUCAGUAGUGCGGUGGGGGACCA UGCGCGAACAUUGUGGUGUUGCACUU

4- Protena codificada (estructura primaria). Cantas solucins posibles hai para a secuencia problema? Porque? Como non sabemos se a cadena problema do ADN da hebra codificante ou da hebra complementaria temos xa duas posibilidades de cadeas de ARN, das cales cada unha pode formar tres tipos distintos de protenas, dependendo onde comece a transcripcins dos tripletes das protenas. Se a protena fose da hebra codificante teramos estas tres posibilidades, mirando o ARN do apartado 3:

Frame RF1 MCDHTKLKILHPV*SAQSSSLGPSQ*CGGGPCANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVSSAVGDHAR TLWCCTYVRPYQAENTASRLICTVKQLRAQSVVRWGTMREHCGVALMCDHTKLKILHPV*SAQSSSLGPS Q*CGGGPCANIVVLH

Frame RF2 CATIPS*KYCIPSDLHSQAA*GPVSSAVGDHARTLWCCTYVRPYQAENTASRLICTVKQLRAQSVVRWGTM REHCGVALMCDHTKLKILHPV*SAQSSSLGPSQ*CGGGPCANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVS SAVGDHARTLWCCT

Frame RF3 VRPYQAENTASRLICTVKQLRAQSVVRWGTMREHCGVALMCDHTKLKILHPV*SAQSSSLGPSQ*CGGGP CANIVVLHLCATIPS*KYCIPSDLHSQAA*GPVSSAVGDHARTLWCCTYVRPYQAENTASRLICTVKQLRAQS VVRWGTMREHCGVAL

Se fose da hebra complementara teramos que referirnos o apartado 2, cuxo ARN : AGUGCAACACCACAAUGUUCGCGCAUGGUCCCCCACCGCACUACUGACUGGGCCCUAAGCUGCUU GACUGUGCAGAUCAGACGGGAUGCAGUAUUUUCAGCUUGGUAUGGUCGCACAUAAGUGCAACAC CACAAUGUUCGCGCAUGGUCCCCCACCGCACUACUGACUGGGCCCUAAGCUGCUUGACUGUGCAG AUCAGACGGGAUGCAGUAUUUUCAGCUUGGUAUGGUCGCACAUAAGUGCAACACCACAAUGUUC GCGCAUGGUCCCCCACCGCACUACUGACUGGGCCCUAAGCUGCUUGACUGUGCAGAUCAGACGGG AUGCAGUAUUUUCAGCUUGGUAUGGUCGCACAUAAGUGCAACACCACAAUGUUCGCGCAUGGUC CCCCACCGCACUACUGACUGGGCCCUAAGCUGCUUGACUGUGCAGAUCAGACGGGAUGCAGUAUU UUCAGCUUGGUAUGGUCGCACAU Desta cadea de ARN seran estas as posibilidades de grupos de protenas:

Frame RF1 SATPQCSRMVPHRTTDWALSCLTVQIRRDAVFSAWYGRT*VQHHNVRAWSPTALLTGP*AA*LCRSDGM QYFQLGMVAHKCNTTMFAHGPPPHY*LGPKLLDCADQTGCSIFSLVWSHISATPQCSRMVPHRTTDWAL SCLTVQIRRDAVFSAWYGRT Frame RF2 VQHHNVRAWSPTALLTGP*AA*LCRSDGMQYFQLGMVAHKCNTTMFAHGPPPHY*LGPKLLDCADQTG CSIFSLVWSHISATPQCSRMVPHRTTDWALSCLTVQIRRDAVFSAWYGRT*VQHHNVRAWSPTALLTGP* AA*LCRSDGMQYFQLGMVAH Frame RF3 CNTTMFAHGPPPHY*LGPKLLDCADQTGCSIFSLVWSHISATPQCSRMVPHRTTDWALSCLTVQIRRDAVF SAWYGRT*VQHHNVRAWSPTALLTGP*AA*LCRSDGMQYFQLGMVAHKCNTTMFAHGPPPHY*LGPKL LDCADQTGCSIFSLVWSH.

5- Estadstica da secuencias a) Porcentaxe de nucletidos Adenina: 112 nucletidos Citosina: 120 nucletidos Guanina: 136 nucletidos Timina: 108 nucletidos

23,52% 25,21% 28,57% 22,68%

b) Porcentaxe de dmeros (parejas de bases diferentes) AA: AC: AG: AT: CA: CC: CG: CT: GA: GC: GG: GT: TA: TC: TG: TT: 24 dimers 28 dimers 32 dimers 28 dimers 48 dimers 28 dimers 20 dimers 24 dimers 20 dimers 44 dimers 32 dimers 40 dimers 19 dimers 20 dimers 52 dimers 16 dimers 5.04% 5.88% 6.72% 5.88% 10.08% 5.88% 4.2% 5.04% 4.2% 9.24% 6.72% 8.4% 3.99% 4.2% 10.92% 3.36%

c) Porcentaxe de tripletes (codones) AAA: AAC: AAG: AAT: ACA: ACC: 8 trimers 4 trimers 8 trimers 4 trimers 8 trimers 12 trimers 1.68% 0.84% 1.68% 0.84% 1.68% 2.52%

ACG: ACT: AGA: AGC: AGG: AGT: ATA: ATC: ATG: ATT: CAA: CAC: CAG: CAT: CCA: CCC: CCG: CCT: CGA: CGC: CGG: CGT: CTA: CTC: CTG: CTT: GAA: GAC: GAG: GAT: GCA: GCC: GCG: GCT: GGA: GGC: GGG: GGT: GTA: GTC: GTG: GTT: TAA: TAC: TAG:

0 trimers 8 trimers 0 trimers 12 trimers 4 trimers 16 trimers 8 trimers 8 trimers 8 trimers 4 trimers 8 trimers 8 trimers 16 trimers 16 trimers 16 trimers 8 trimers 4 trimers 0 trimers 8 trimers 4 trimers 4 trimers 4 trimers 0 trimers 0 trimers 16 trimers 8 trimers 8 trimers 8 trimers 0 trimers 4 trimers 16 trimers 4 trimers 16 trimers 8 trimers 4 trimers 4 trimers 16 trimers 8 trimers 4 trimers 12 trimers 20 trimers 4 trimers 0 trimers 8 trimers 8 trimers

0% 1.68% 0% 2.52% 0.84% 3.36% 1.68% 1.68% 1.68% 0.84% 1.68% 1.68% 3.36% 3.36% 3.36% 1.68% 0.84% 0% 1.68% 0.84% 0.84% 0.84% 0% 0% 3.36% 1.68% 1.68% 1.68% 0% 0.84% 3.36% 0.84% 3.36% 1.68% 0.84% 0.84% 3.36% 1.68% 0.84% 2.52% 4.2% 0.84% 0% 1.68% 1.68%

TAT: TCA: TCC: TCG: TCT: TGA: TGC: TGG: TGT: TTA: TTC: TTG: TTT:

3 trimers 8 trimers 4 trimers 0 trimers 8 trimers 8 trimers 24 trimers 8 trimers 12 trimers 7 trimers 0 trimers 8 trimers 0 trimers

0.63% 1.68% 0.84% 0% 1.68% 1.68% 5.04% 1.68% 2.52% 1.47% 0% 1.68% 0%

6- Enderezo usado para resolver o exercicio http://www.dnai.org/geneboy/