1. Students will observe plant, animal, protist and bacterial cells under a microscope. They will examine Elodea plant cells and cheek cells from their own mouths, with and without staining.
2. For Elodea cells, students will draw cells at different magnifications and identify chloroplasts and cell walls. They will compare their observations to textbook diagrams of plant cells.
3. For cheek cells, students will sketch unlabeled and stained cells, noting differences between the two preparations. They will also compare cheek cells to Elodea cells and describe the differences between plant and animal cells.
1. Students will observe plant, animal, protist and bacterial cells under a microscope. They will examine Elodea plant cells and cheek cells from their own mouths, with and without staining.
2. For Elodea cells, students will draw cells at different magnifications and identify chloroplasts and cell walls. They will compare their observations to textbook diagrams of plant cells.
3. For cheek cells, students will sketch unlabeled and stained cells, noting differences between the two preparations. They will also compare cheek cells to Elodea cells and describe the differences between plant and animal cells.
1. Students will observe plant, animal, protist and bacterial cells under a microscope. They will examine Elodea plant cells and cheek cells from their own mouths, with and without staining.
2. For Elodea cells, students will draw cells at different magnifications and identify chloroplasts and cell walls. They will compare their observations to textbook diagrams of plant cells.
3. For cheek cells, students will sketch unlabeled and stained cells, noting differences between the two preparations. They will also compare cheek cells to Elodea cells and describe the differences between plant and animal cells.
1. Students will observe plant, animal, protist and bacterial cells under a microscope. They will examine Elodea plant cells and cheek cells from their own mouths, with and without staining.
2. For Elodea cells, students will draw cells at different magnifications and identify chloroplasts and cell walls. They will compare their observations to textbook diagrams of plant cells.
3. For cheek cells, students will sketch unlabeled and stained cells, noting differences between the two preparations. They will also compare cheek cells to Elodea cells and describe the differences between plant and animal cells.
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Unit 3 Lab
Today you will: Observe plant, animal, protist and bacterial cells. Be able to identify cellular structures (membrane, nucleus, etc) Advice: Do not rush through this lab! Materials: Paper Lab eport Pen!Pencil Microscope Microscope "lides #over "lips $lodea Leaves Met%ylene Blue Part I. Observations of Plant Cells &irst, become familiar wit% t%e cellular structure of a leaf of Elodea, a water plant, by studyin' a prepared slide. T%e leaf %as many layers of cells, especially in t%e t%ic(est central part of t%e leaf. )%en many layers of cells overlap, t%e details of small structures wit%in eac% cell become %idden. Therefore, it is most important for you to find cells near the edge of the leaf. Choose cells that are large and easy for you to observe clearly. Make a hypothesis s(etc% w%at you t%in( t%e plant cell will loo( li(e. Procedure )et Mount: *et a leaf of livin' Elodea. #%oose one of t%e youn'est (smallest) leaves from a spri' of Elodea. Ma(e sure t%at t%e leaf is bri'%t 'reen and %as t%in, almost see+t%rou'% ed'es. Tear one leaf off t%e Elodea, tear it in %alf, and place it on a clean microscope slide. ,dd -+. drops of water to t%e leaf and place one cover glass on top of t%e leaf. ! CA"!#$%& cover glasses tend to stick together. /f you %ave two cover 'lasses stuc( to'et%er you will not be able to use t%e -001 ob2ective on your specimen. Collect Data $1amine t%e wet mount slide of Elodea, first usin' t%e 31, ob2ective, t%en wit% t%e -01, and t%en t%e 301. 4otice t%at t%ere are many different layers of cells present. ,t eac% level of t%e microscope (31, -01 and 301) ma(e a drawin' of . cells. Ma(e sure to identify c%loroplasts and cell walls. Analy'e the Data 5o t%e cells loo( li(e t%e plant cells t%at you see in t%e te1tboo(6 )%y or w%y not6 Conclusions )%at is t%e benefit of bein' able to see t%e cells wit% %i'%er ma'nification6
,.
B.
,n Elodea #ell. (,) 7pper surface of t%e cell (B) Middle of t%e cell. T%e dis(+s%aped t%in's are c%loroplasts located in t%e cytoplasm alon' t%e cell wall. 4otice t%e lac( of c%loroplasts in t%e center of t%e cell w%ic% is t%e vacuole.
#.
,n Elodea #ell. (#) Middle of t%e cell, %i'%er ma'nification t%an , and B Below: epresentative c%ee( cells after Met%ylene Blue stainin'.
Part II. Observations of Ani(al Cells $nstained ,. T%e 8uman #%ee( #ell Make a )ypothesis )%at do you t%in( your c%ee( cells will loo( li(e6 Ma(e a s(etc%. Procedure Prepare a wet mount of your c%ee( cells: -. Put a drop of water on a slide. .. *ently scrape t%e inside of your c%ee( wit% t%e flat side of a toot%pic(. "crape li'%tly. 9. "tir t%e end of t%e toot%pic( into t%e water and t%row t%e toot%pic( away. 3. Place a coverslip onto t%e slide. (put one ed'e of t%e coverslip on t%e slide and t%en 'ently drop t%e ot%er ed'e into place. :. Locate cells under low power (-01). #ells may be %ard to see, but t%ey can be found. Ma(e sure to use t%e ad2ustment (nob. ;. Once you t%in( you %ave located a cell, switc% to %i'% power. ( emember, do 4OT use t%e coarse ad2ustment (nob). Collect Data "(etc% two cells. Ma(e sure to label t%e cell membrane and t%e nucleus. #urther *uestions 8ow are t%e elodea cells and t%e c%ee( cells different6 Ma(e sure you answer t%e <uestion in words and use pictures to furt%er e1plain t%e answers. Part II. Observations of Ani(al Cells +tained Make a )ypothesis )%at do you t%in( your c%ee( cells will loo( li(e wit% stain6 Ma(e a s(etc%. Procedure Prepare a wet mount of your c%ee( cells, wit% stain: -. Put two drops of water on a slide. "ee Ms. #ornic( to %ave a drop of met%ylene blue (0..=) put on a new slide. #aution: met%ylene blue will stain clot%es and s(in. Put two drops of water on t%e slide too. .. ,'ain, 'ently scrape t%e inside of your c%ee( wit% t%e flat side of a toot%pic( and stir t%e end of t%e toot%pic( into t%e stain and t%row t%e toot%pic( away. 9. Place a coverslip onto t%e slide. 3. "witc% to low power (-01). #ells s%ould be visible, but t%ey will be small and loo( li(e nearly clear purplis% blobs. /f you are loo(in' at somet%in' dar( purple, it is probably not a cell. :. Once you t%in( you %ave located a cell, switc% to %i'% power. Collect Data "(etc% at least two cells. Label t%e nucleus, cytoplasm, and cell membrane.
Analy'e the data )%at did t%e stain allow you to do6 5id t%e cells loo( li(e t%e animal cells in t%e te1tboo(6 Wash off all slides and coverslips. Clean up your work area!
