Ti-E Instruction Manual

Inverted Microscope
Instructions
M450 E 08.1.NF.2
*M450EN02*

1
Introduction

Thank you for purchasing a Nikon product.
This instruction manual is written for users of Nikon Inverted Microscope Eclipse Ti-E and Ti-E/B.
To ensure correct usage, read this manual carefully before operating the product.
No part of this manual may be reproduced or transmitted in any form without prior written permission from
Nikon.
The contents of this manual are subject to change without notice.
Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may
remain. If you note any points that are unclear or incorrect, please contact your nearest Nikon
representative.
Some of the equipment described in this manual may not be included in the set you have purchased.
If you intend to use any other equipment with this product, read the manual for that equipment too.
When using TI-HUBC/A Hub Controller A with the microscope, also refer to the instructions manual
provided with the hub controller.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the
equipment may be impaired.

2
Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

Warning and Caution Symbols in this Manual
Although this product is designed and manufactured to be completely safe during use, incorrect usage or
failure to follow the safety instructions provided may cause personal injury or property damage. To ensure
correct usage, read this manual carefully before using the product. Do not discard this manual and keep it
handy for easy reference.
Safety instructions in this manual are marked with the following symbols to highlight their importance. For
your safety, always follow the instructions marked with these symbols.
Symbol Description

Warning
Disregarding instructions marked with this symbol may lead to serious injury or death.

Caution
Disregarding instructions marked with this symbol may lead to injury or property
damage.

Symbols on the Product
The symbols on the product indicate the need for caution at all times during use. Before operating a part
labeled with the following symbols, refer to the instruction manual and read the relevant instructions.
Symbol Meaning

CAUTION: HOT
This symbol can be found on the top of the dia pillar illuminator and on the 12V 100W
lamphouse, and cautions the following:
The lamp and the lamphouse will be extremely hot while and immediately after using
the lamp.
To avoid the risk of burns, do not touch the lamp and the lamphouse while or
immediately after using the lamp.
When replacing the lamp, wait for the lamp and the lamphouse to cool sufficiently.

Biohazard
This symbol can be found on the upper part of the microscope, and cautions the
following:
The product may become biohazardous if a specimen is spilled onto the product.
To avoid exposure to biohazard, do not touch contaminated parts with your bare
hands.
Decontaminate the contaminated parts according to the standard procedures for your
facility.

General Caution
This symbol can be found on the top of the nosepiece protection plate on Ti-E and
Ti-E/B, and on top of the PFS6 nosepiece protection plate on the TI-ND6-PFS PFS
Motorized Nosepiece, and cautions the following:
To avoid injury (i.e. by getting your fingers caught), be sure to attach the protection
plate correctly.

Safety Precautions

3
LED Safety
The PFS Motorized Nosepiece (TI-ND6-PFS Perfect Focus Unit) uses light in the near-infrared region (IR
wavelength) emitted from an infrared LED to control the focus. When using the PFS Motorized Nosepiece
with the product, the microscope system conforms to the EU standard EN60825-1: 2001 and the
international standard IEC60825-1: 2001. The product is classified as a Class 1 LED product.
Attempting to control or adjust the product in manners not described in this manual may result in exposure to
hazardous LED light.
Safety Labels on TI-ND6-PFS Perfect Focus Unit
Safety labels are affixed to the bottom of the TI-ND6-PFS Perfect Focus Unit.

CLASS 1 LED PRODUCT
IEC/EN60825-1 : 2001
CAUTION - CLASS 1M INVISIBLE
LED RADIATION WHEN OPEN
DO NOT VIEWDIRECTRLY WITH
OPTICAL INSTRUMENTS
5 9 3 9 0 1
JAPAN
TI-ND6-PFS

PFS Motorized Nosepiece (bottom view)
(1) Caution label
CAUTION - CLASS 1M INVISIBLE
LED RADIATION WHEN OPEN
DO NOT VIEW DIRECTRLY WITH
OPTICAL INSTRUMENTS

(2) Class 1 LED product label
CLASS 1 LED PRODUCT
IEC/EN60825-1 : 2001

(3) Safety standards label
5 9 3 9 0 1
JAPAN
TI
-
ND6
-
PFS

Note that under normal use, infrared light is emitted from the position shown in the figure below.
OUT
DICHROIC MIRROR
IN

Infrared light emission port
(3) Safety standards label (2) Class1 LED
product label
(1) Caution label
Safety Precautions

4

Warning

1. Intended application of the product
The product is intended mainly for microscopic observations and micromanipulations of living
cells and tissues under diascopic or episcopic illumination. It is designed for the purposes of
experimentations and observations at hospitals or laboratories in the fields of genetics,
immunology, physiology, pharmacology, neurology, cellular biology, and molecular biology.
The product is classified as an in-vitro diagnostic medical device.
2. Do not disassemble.
Disassembling this product may result in electric shock or malfunction. Malfunctions and damage
due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated
in this manual. If you experience problems with the product, contact Nikon.
3. Read the instructions thoroughly.
To ensure safety, thoroughly read this manual and the manuals for other equipment to be used
with this product. In particular, be sure to follow the warnings and cautions at the beginning of the
manuals.
4. Check the input voltage.
The microscope body uses an AC adapter as its power source. The dia illumination lamp uses
one of two types of power supply devices.
AC adapter for the microscope body:
The AC adapter for the microscope can be used with 100 to 240 VAC at 50-60 Hz, and can be
used with most wall outlets in the world. Under normal use, you will not need to pay particular
attention to the supplied power voltage.
TI-PS100W Power Supply:
TI-PS100W Power Supply can be used with 100 to 240 VAC at 50/60 Hz, and can be used
with most wall outlets in the world. Under normal use, you will not need to pay particular
attention to the supplied power voltage.
TE-PS30W Power Supply A or TE-PSE30 Power Supply A:
The input voltage ratings are indicated on the rear panel of the power supply device. Before
connecting the power cord, check that the indicated input voltage matches the voltage of the
wall outlet. If the indicated input voltage does not match your regional voltage supply, do not
use the power supply device, and contact Nikon. Use of a power supply device with the
inappropriate voltage rating may result in overheating or fire due to overcurrent, and may also
cause damage the power supply device and connected devices.
5. Use the specified AC adapter only.
The product is powered by an AC adapter. Be sure to use the specified AC adapter with the
product. Use of other AC adapters may result in malfunction, overheat, or fire.
See Chapter 7, Specifications for the specified AC adapter.
Place the AC adapter in a well-ventilated area to prevent malfunction and fire. Obstacle
covering or placed on the AC adapter may hinder the dissipation of heat and cause the AC
adapter to become abnormally hot.
To prevent malfunctions and errors, turn off the POWER switch on the Ti-E or Ti-E/B
microscope body (set the POWER switch to the OFF side) before connecting the AC
adapter.

Safety Precautions

5

Warning

6. Cautions on the power cord
Be sure to use the specific power cords for the AC adapter and the power supply device. Use of
other power cords may result in malfunction, overheat, or fire.
See Chapter 7, Specifications for the specified power cord.
To prevent electric shock, turn off the power switches on the Ti-E or Ti-E/B microscope body
and on the power supply device before connecting or disconnecting the power cord. On Ti-E
and Ti-E/B, set the POWER switch to the OFF side. On the power supply, set the POWER
switch to the O side.
Note that the power supply device is classified as Class I for electric shock protection. Be sure
to connect it to a protective ground terminal.
7. Check the combination of dia pillar illuminator, lamp, and power supply device.
The combination of the dia pillar illuminator and the power supply device is specified based on
the lamp ratings (12 V 100 W or 6 V 30 W) and the power voltage. Use them in the correct
combination according to the instructions on page 56. Use of the devices in a wrong combination
may result in malfunction, overheat, or fire.
8. Cautions on heat from the light source
The lamp and the lamphouse become extremely hot when the lamp is turned on. Follow the
cautions below to prevent burns and fire.
To avoid burns, do not touch the lamp and the lamphouse while the lamp is on or for
approximately thirty minutes after it has been turned off.
To avoid the risk of fire, do not place fabric, paper, or highly flammable volatile materials (i.e.
gasoline, petroleum benzine, paint thinner, and alcohol) near the lamphouse while the lamp is
on or for about thirty minutes after it has been turned off.
Do not block the air vents on the lamphouse. If any obstacle covers the lamphouse or
something is placed on the lamphouse, the heat dissipation is inhibited and the lamphouse
becomes abnormally hot.
The bottom of the power supply device becomes hot during use. Do not cover the air vents on
the side of the power supply device.
9. Cautions on lamp replacement
When replacing the lamp, wait approximately thirty minutes after turning off the lamp, and
make sure that the lamp and the lamphouse have cooled sufficiently.
To prevent electric shock and product damage, turn off the power switch on the microscope
body and on the power supply (set each POWER switch to the OFF/O side) and unplug
the power cord from the wall outlet before replacing the lamp.
After replacing the lamp, close and secure the lamphouse cover. Never use the product with
the lamphouse cover left open.
Do not break the used lamp. It should be disposed of as an industrial waste, according to the
local regulations and rules.

Safety Precautions

6

Warning

10. Cautions on motorized devices
The product can rotate and focus (raise and lower) the PFS Motorized Nosepiece with motors.
The product can also have various motorized devices attached. When TI-HUBC/A Hub Controller
A is used with the product, the system can be controlled with a Ti series controller or a PC.
To prevent injuries, pay attention to the following when operating motorized devices:
Before operating the product, check the whole microscope system, and make sure that all
moving parts can be operated safely.
Your hands and fingers may be caught and injured by the nosepiece, objectives, stage, parts
arranged on the stage, or specimen containers. Keep your hands away from these devices
during operation.
Check the optical path of the whole microscope system before operating the shutter on the
illuminator or adjusting the brightness. The light source emits a very strong light. If the optical
path is not set up correctly, the illumination light may leak out or enter the objective, and
cause an eye injury.
11. Notes on handling hazardous specimens
The product is intended mainly for microscopic observations and micromanipulations of
specimens such as living cells and tissues in a Petri dish. Note the following when handling the
specimens:
Before handling a specimen, check whether it is hazardous. Wear rubber gloves when
handling hazardous (i.e. potentially infectious) specimens.
Be careful not to spill the specimen. If a specimen is spilt onto the microscope, decontaminate
according to the standard procedures for your facility.
12. Notes on handling flammable solvents
The following flammable solvents are used with the product:
Immersion oil (Nikon Immersion Oil for oil immersion objectives)
Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)
Petroleum benzine (for wiping off the immersion oil)
Medical alcohol (for disinfecting the microscope)
Never hold a flame near these solvents. When using a solvent, thoroughly read the instructions
provided by the manufacturer, and handle correctly and safely. Note the following precautions
when using solvents with the product.
Keep solvents away from the lamp, the lamphouse, the power supply device, and any other
parts that may become hot.
Keep solvents away from the product and its surroundings when turning on/off the power
switch or plugging/unplugging the power cord.
Be careful not to spill the solvents.

Safety Precautions

7

Caution

1. Turn off the power during installation, assembly, connection/disconnection of cables,
and maintenance.
To prevent electric shock and fire, be sure to turn off the power switch on the microscope body
and the power supply (set the POWER switches to the OFF side or the O side) and unplug
the power cords before installing or assembling the product, connecting or disconnecting cables,
replacing the lamp, or performing maintenance tasks such as cleaning of the lenses.
2. Do not wet the product or allow intrusion of foreign matters.
Do not wet or spill liquids onto the product, as it may result in malfunction, overheat, or electric
shock. If water or other liquids are accidentally spilled onto the product, immediately turn off the
power switch (set the POWER switch to the OFF side or the O side) and unplug the power
cord from the wall outlet. Then, wipe off the liquid with a piece of dry cloth. Intrusion of foreign
matters into the product may also result in malfunctions. If liquids or foreign matters enter the
product, do not use the product, and contact Nikon.
3. Weak electromagnetic waves
The product emits weak electromagnetic waves. Do not install the product near precision
electronic devices to avoid affecting their performance. If signal reception by a TV or radio is
affected, move the TV or radio set away from the product.
4. Cautions on moving the product
When carrying the product, hold the product firmly by the bottom front recess and the bottom
rear.
When moving the product, do not hold by the focusing knobs, eyepiece tube, stage, or dia
pillar illuminator. The parts may become detached and cause the product to fall, and may also
result in malfunctions and loss of precision.
5. Cautions on assembling the product
Take care to avoid pinching your fingers and hands.
Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will
degrade the microscope image. When assembling the product, be careful not to scratch or
directly touch the optical components.
6. Cautions on the protruding rack of the Rectangular Mechanical Stage
The stage rack of TI-SR Rectangular Mechanical Stage will protrude when the stage is operated.
When operating the focus knobs or the nosepiece, be careful not to strike your hands against the
rack. Contact with the edges of the rack may result in injury.
7. Disposal
To avoid biohazard risks, dispose of the product as contaminated equipment, according to the
standard procedures for your facility.

Safety Precautions

8
Notes on Handling the Product
1. Handle with care.
The product is a precision optical instrument. Handle the product with care and avoid physical shocks
and vibrations.
In particular, the precision of objectives may be lost by even weak physical shocks.
2. Installation location and storage location
The product is a precision optical instrument. Use or storage of the product under inappropriate
conditions may result in malfunctions or loss of precision. The following conditions must be considered
for the installation location and the storage location.
Install the product in a location with a temperature of 0 to +40C and a relative humidity of 85% or
less (no condensation).
Store the product in a location with a temperature of -20 to +60C and a relative humidity of 90% or
less (no condensation).
Use or storage of the product in a hot or humid location may result in molding of or condensation on
the lenses, loss of precision, and malfunctions.
Install the product in a place with little dust and dirt.
When storing the product, place a cover over the product to protect it from dust.
Install the product in a place with little vibration.
Install and store the product on a level and sturdy table or stage that can bear the weight of the
product.
Install the product in a location with minimal exposure to hazards in the event of earthquakes and
other potential disasters. If necessary, secure the product to the working desk or other heavy and
stable items with a strong rope or other means, so as to prevent it from falling.
Avoid placing the product in direct sunlight or immediately under the room lights.
The image quality is degraded in a bright environment due to the extraneous light entering the
objective. A room light immediately above the microscope may also enter the objective as
extraneous light, especially when using a condenser lens with a longer working distance (i.e. ELWD
and LWD). In this case, it is recommended that you turn off the room light above the microscope.
Install the product at least 10 cm away from the surrounding walls.
When using TI-DH Dia Pillar Illuminator 100W, install the product at least 15 cm away from the wall,
so that the caution labels on the dia pillar illuminator and the lamphouse are visible. Note that TI-DH
Dia Pillar Illuminator 100W can be inclined backward to secure working space. To utilize this function,
install the product so that there is enough space between the product and the wall for the dia pillar
illuminator to be inclined.
Do not install the product in a closed space such as a locker or a cabinet.
Do not place items on the product.
Install the product so that the power cords to the AC adapter and the power supply can be
unplugged immediately in case of an emergency.
3. Notes on handling optical parts
Scratches and dirt (i.e. fingerprints) on optical components such as lenses and filters will degrade the
microscope image.
Handle the optical components with care, so as not to damage them. If they require cleaning, see
Chapter 6, Daily Maintenance.
4. Notes on handling the lamp
Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp may result in
uneven illumination and reduce the service life of the lamp. Wear gloves or use other pieces of clothes
when handling the lamp.
Safety Precautions

9
5. Notes on using the focus knobs
Never rotate the left and right focus knobs on the microscopes in the opposite directions at the same
time. Doing so may damage the product.
Do not rotate the focus knobs past their limit. Doing so may damage the product.
6. Protecting the ports.
The microscope has multiple ports. To keep out extraneous light and dust, attach the provided caps to
the ports that are not in use.
7. Caution on motorized devices
Do not force movements on the motorized parts by hand.
8. Note on vibrations caused by motorized operations
This product is designed to minimize the amount of vibration during motorized operation. However,
depending on the application, the vibration may have an effect on the microscopy.
9. Note on the Z-axis position display
Ti-E and Ti-E/B microscopes can display the Z-axis position on the front status display panel. Note,
however, that the precision of the value is not guaranteed, as Ti-E and Ti-E/B are not designed as
measurement machines.

10
Contents
Introduction...................................................................................................................................................... 1
Safety Precautions .......................................................................................................................................... 2
Warning and Caution Symbols in this Manual ............................................................................................................ 2
Symbols on the Product ............................................................................................................................................. 2
LED Safety................................................................................................................................................................. 3
Notes on Handling the Product .................................................................................................................................. 8
1. Part Names............................................................................................................................................. 12
1.1 Ti-E, Ti-E/B.................................................................................................................................................... 13
1.2 Microscope Base .......................................................................................................................................... 14
1.2.1 Microscope Base............................................................................................................................ 14
1.2.2 Operation Panels............................................................................................................................ 15
1.2.3 Connector Panels........................................................................................................................... 16
1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces.................................................................................... 17
1.4 PFS Motorized Nosepiece and PFS Offset Controller .................................................................................. 19
1.5 Stage............................................................................................................................................................. 20
1.6 Dia Pillar Illuminator ...................................................................................................................................... 21
1.6.1 TI-DH Dia Pillar Illuminator 100W................................................................................................... 21
1.6.2 TI-DS Dia Pillar Illuminator 30W..................................................................................................... 21
1.7 Condenser .................................................................................................................................................... 22
1.7.1 TI-C Condenser Turret (System Condenser).................................................................................. 22
1.7.2 ELWD-S Condenser ....................................................................................................................... 23
1.7.3 High NA Condenser Holder ............................................................................................................ 23
1.8 Power Supply................................................................................................................................................ 24
1.8.1 TI-PS100W Power Supply.............................................................................................................. 24
1.8.2 TE-PS30W Power Supply A (for 100-120V) TE-PSE30 Power Supply A (for 230V)....................... 25
1.9 AC Adapter ................................................................................................................................................... 26
2. Microscopy............................................................................................................................................. 27
2.1 Introduction to Microscopy ............................................................................................................................ 28
2.2 Bright-Field (BF) Microscopy......................................................................................................................... 30
2.3 Phase Contrast (Ph) Microscopy .................................................................................................................. 38
2.4 External Phase Contrast Microscopy............................................................................................................ 43
2.5 In-focus Observation with PFS...................................................................................................................... 50
3. Operation................................................................................................................................................ 54
3.1 Power On/Off ................................................................................................................................................ 55
3.1.1 Power On ....................................................................................................................................... 55
3.1.2 Power Off ....................................................................................................................................... 55
3.2 Dia Illumination Operation............................................................................................................................. 56
3.2.1 Combination of Lamp, Dia Pillar Illuminator, and Power Supply..................................................... 56
3.2.2 Power Supply Setting..................................................................................................................... 56
3.2.3 Dia Illumination Lamp Operation .................................................................................................... 57
3.2.4 Brightness Adjustment with ND Filters ........................................................................................... 57
3.3 Controls on the Microscope Body ................................................................................................................. 58
3.3.1 Front Operation Panel .................................................................................................................... 58
3.3.2 Left Operation Panel ...................................................................................................................... 60
3.3.3 Right Operation Panel .................................................................................................................... 61
3.4 Optical Path Selection................................................................................................................................... 62
3.4.1 For Ti-E .......................................................................................................................................... 62
3.4.2 For Ti-E/B....................................................................................................................................... 62
3.4.3 Eyepiece Base Unit Options........................................................................................................... 62
3.5 Filter Operation ............................................................................................................................................. 63
3.6 Field Diaphragm Operation........................................................................................................................... 64
3.7 Aperture Diaphragm Operation..................................................................................................................... 65

Contents

11
3.8 Condenser Operation.................................................................................................................................... 67
3.8.1 TI-C Condenser Turret (System Condenser).................................................................................. 67
3.8.2 ELWD-S Condenser ....................................................................................................................... 68
3.8.3 High NA Condenser Holder ............................................................................................................ 68
3.9 Eyepiece Tube Operation ............................................................................................................................. 69
3.9.1 Diopter Adjustment ......................................................................................................................... 69
3.9.2 Interpupillary Distance Adjustment ................................................................................................. 69
3.9.3 Eyepiece Tube Shutter Operation .................................................................................................. 70
3.9.4 Bertrand Lens Operation................................................................................................................ 70
3.10 Focusing Mechanism Operation ................................................................................................................... 71
3.10.1 Focus Knob Operation ................................................................................................................... 71
3.10.2 Z-RESET Switch Operation............................................................................................................ 72
3.10.3 Retraction and Refocusing of Objective ......................................................................................... 73
3.11 Objective Operation ...................................................................................................................................... 74
3.11.1 Phase Contrast Objectives............................................................................................................. 74
3.11.2 Cover Glass Thickness .................................................................................................................. 74
3.11.3 Objectives with Correction Ring ..................................................................................................... 75
3.11.4 Oil Immersion Objectives ............................................................................................................... 76
3.11.5 Water Immersion Objectives .......................................................................................................... 78
3.12 Pillar Illuminator 100W Operation ................................................................................................................. 79
3.12.1 Condenser Refocusing Clamp ....................................................................................................... 79
3.12.2 Condenser Mount Rotation ............................................................................................................ 79
3.12.3 Pillar Inclination .............................................................................................................................. 80
3.12.4 Device Attachment Screw Holes .................................................................................................... 80
3.13 Rectangular Mechanical Stage Operation..................................................................................................... 81
3.14 PFS (Perfect Focus System) Operation........................................................................................................ 82
3.14.1 PFS Overview ................................................................................................................................ 82
3.14.2 Starting and Stopping In-focus Observation with the PFS.............................................................. 85
3.14.3 Offset Adjustment ........................................................................................................................... 87
3.14.4 Registration and Restoration of Offset ........................................................................................... 88
3.14.5 If the Objective is Changed ............................................................................................................ 89
3.14.6 If the PFS Function Does Not Start ................................................................................................ 89
4. Assembly................................................................................................................................................ 90
5. Troubleshooting ...................................................................................................................................119
5.1 Image Viewing ............................................................................................................................................ 119
5.2 Operation.................................................................................................................................................... 121
5.3 Electrical System ........................................................................................................................................ 121
5.4 Perfect Focus System................................................................................................................................. 122
6. Daily Maintenance ............................................................................................................................... 124
6.1 Cleaning Optical Components .................................................................................................................... 124
6.2 Cleaning the Microscope Body ................................................................................................................... 124
6.3 Disinfecting the Microscope........................................................................................................................ 124
6.4 Storage ....................................................................................................................................................... 124
6.5 Periodic Inspections (Paid Service) ............................................................................................................ 124
7. Specifications ...................................................................................................................................... 125
7.1 Microscope (Ti-E or Ti-E/B) with TI-DH Dia Pillar Illuminator 100W............................................................... 125
7.2 Microscope (Ti-E or Ti-E/B) with TI-DS Dia Pillar Illuminator 30W................................................................. 126
7.3 PFS Motorized Nosepiece and PFS Offset Controller ................................................................................ 127
7.4 AC Adapter ................................................................................................................................................. 129
7.5 Power Cord................................................................................................................................................. 129
7.5.1 Power Cord for the AC Adapter .................................................................................................... 129
7.5.2 Power Cord for the Power Supply ................................................................................................ 129
7.6 Safety Standards Compliance..................................................................................................................... 130

12
Part Names
1

This chapter describes the name of each part of the product.
When using the product for the first time, refer to this chapter and check the name and the position of each
part. Also refer to this chapter for names and positions of the controls whenever necessary.
The components of the microscope can be selected to suit the application. However, the lamp, the dia
pillar illuminator, and the power supply device must be used in specific combinations. Do not use these
devices in an inappropriate combination. For combinations of the lamp, the dia pillar illuminator, and the
power supply device, refer to page 56.
For details on microscopy procedures, see Chapter 2, Microscopy. For details on the operation of each
part, see Chapter 3, Operation.
If the microscope has not been assembled yet, first refer to Chapter 4, Assembly.
When using the TI-HUBC/A Hub Controller A with the Ti-E or Ti-E/B microscope body, motorized devices
can be controlled with an external controller. For details, refer to the instruction manual provided with
TI-HUBC/A Hub Controller A.

Chapter 1 Part Names
1.1 Ti-E, Ti-E/B
13
1.1 Ti-E, Ti-E/B
The illustrations below show the Ti-E microscope body with the following accessories:
TI-DH Dia Pillar Illuminator 100W, TI-C Condenser Turret, D-LH/LC Precentered Lamphouse LC, 12 V
100 W halogen lamp, TI-PS100W Power Supply, TI-SR Rectangular Mechanical Stage, TI-T-B Eyepiece
Base Unit, TI-TD Eyepiece Tube B, CFI 10x eyepieces, TI-ND6-PFS Perfect Focus Unit, objectives, etc.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
P
F
S
D O W
N
U
P
P
F
S
OFFSET
CONTROLLER
FINE
COARSE

Figure 1.1-1 Ti-E

L80
EYE
DISPLAY
MEMORY
PFS
ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse
Fine
ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 1.1-2 Ti-E (left view)
* Attach a protective cap on the side port when the side port is not in use.

Left operation panel
Left side port *
Focus knob
Dia illumination lamphouse
(Figure: D-LH/LC)
Filter sliders
(both sides, 4 total)
Dia pillar illuminator
(Figure: TI-DH)
Condenser mount
Nosepiece (Figure: TI-ND6-PFS)
Stage knob
Right side port *
Focus knob
PFS Offset Controller
Front operation panel
Power supply
(Figure: TI-PS100W)
Eyepiece base unit
(Figure: TI-T-B)
Eyepiece tube (Figure: TI-TD)
Eyepieces (both sides)
Field diaphragm knob
Condenser lens
Condenser turret (Figure: TI-C)
Condenser centering knob
Aperture diaphragm open/close lever
Condenser focus knobs
(both sides)
Objectives
Stage ring (concentric ring)
Right operation panel
Microscope base
(Figure: Ti-E)
Right connector panel
Stage (Figure: TI-SR)
1.2 Microscope Base
14
1.2 Microscope Base
1.2.1 Microscope Base
To keep out extraneous light and dust, be sure to attach the provided caps to all ports not in use.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS
OFFSET

Figure 1.2-1 Microscope base

Left view
Coarse
Fine
ExFine
Obj.
ON/ OFF
6V30W
MAX. MIN.
12V100W

Figure 1.2-2 Microscope base (left view)

Epi illumination
field diaphragm
slider mount
Focus knob
Left side port
Dia pillar illuminator mount
(A carrying handle is
attached for transportation.)
Connector panel (rear)
Right side port
Focus knob
Bottom port (on the bottom, Ti-E/B only)
Status display panel
Eyepiece base unit mount
Nosepiece mount
Cover
Analyzer slot
(A dummy slider is attached
for transportation.)
Connector panel (right side)
Filter turret mount
Intermediate magnification
selector knob (1.5x 1x)
1.2 Microscope Base
15
1.2.2 Operation Panels


L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1
.5
X

Figure 1.2-3 Front operation panel

Coarse
Fine
ExFine
Obj.
ON/ OFF
6V30W
MAX. MIN.
12V100W

Figure 1.2-4 Left operation panel

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape

Figure 1.2-5 Right operation panel

Z-RESET switch
(Z-axis position reset)
Intermediate magnification selector dial
(1.5X 1X
Optical path selector switches/indicators
Ti-E : EYE, L100, R100, L80
Ti-E/B : EYE, L100, R100, B100
BRIGHTNESS switch
(display brightness selector)
Status display panel (20x2)
DISPLAY switches
(display item selector)
Obj. switch
(objective selector)
Coarse/Fine/ExFine
switch/indicator
(focus knob resolution
selector/indicator)
Dia illumination lamp
ON/OFF switch
brightness control knob
Focus knob
Epi Shutter switch
(epi illumination shutter operation)
Coarse/Fine/ExFine
switch/indicator
selector/indicator)
Focus knob
FL Block switch
(filter block selector)
Refocus switch
(objective refocusing)
Escape switch
(objective retraction)
PFS control switches/indicators
FOCUS indicator
(PFS function status)
PFS-ON switch/indicator
(PFS ON/OFF selector/indicator)
PFS-MEMORY switch/indicator
(offset memory/indicator)
PFS-RECALL switch/indicator
(offset recall)

1.2 Microscope Base
16
1.2.3 Connector Panels

INTERLOCK
PIEZO
PFS
OFFSET

Figure 1.2-6 Right connector panel
Rear connector panel

MIC CONTROL
POWER
OFF ON
LAMP CTRL
DC24V IN
USB
Gepruftes Medizinprodukt Approved medical device Freiwillige Produktprufung R
5 3 1 0 0 1
This device complies with Part 15 of the FCC Rules.Operation is subject to the
following two conditions:
(1) this device may not cause harmful interference,and
(2)
EQUIPMENT 4N75 MODEL : Ti-E
INSPECTION TUV Rheinland Product Safety JAPAN
this device must accept any interference received, including interference
that may cause undesired operation.
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numrique de la classe A est conforme la norme NMB-003 du Canada. TOKYO, JAPAN

Figure 1.2-7 Microscope base (rear view)

Epi-fl illumination mount
DC24V IN connector
(for AC adapter)
MIC CONTROL connector
(for Hub Controller A)
PFS connector
(for PFS motorized nosepiece)
OFFSET connector
(for PFS offset controller)
INTERLOCK connector
(for external laser controller)
PIEZO connector
(for PIEZO motorized stage)
USB connector (for PC)
LAMP CTRL connector
(for dia illumination power
supply)
POWER switch
Hub Controller A attachment
screw holes (x4)

Epi-fl illumination attachment
screw holes (x4)
Grooves for
motorized units wiring
(both sides)
1.3 Eyepiece Base Unit, Eyepiece Tube, and Eyepieces
17
The following eyepiece base units, eyepiece tubes, and eyepieces can be mounted on the observation port
of the microscope.

TI-T-B Eyepiece Base Unit

Figure 1.3-1 TI-T-B Eyepiece Base Unit

This is a basic type eyepiece tube base.
There is a dovetail (truncated cone) joint at the top to
attach an eyepiece tube, which is used for visible
observation.
If you dont need visible observation, the mount can
be left unused. In this case, attach a cover to the
dovetail joint.

TI-T-BS Eyepiece Base Unit (with Side Port)

S
ID
E
EYE

Figure 1.3-2 TI-T-BS Eyepiece Base Unit
(with Side Port)

This is an eyepiece tube base with a camera port.
Use the optical path selector knot at right to change
the optical path between the eyepiece side and the
side port side.
A direct C-mount adapter is attached to the side port
for camera connection.

TI-T-BPH Eyepiece Base Unit (for External Phase Contrast)

S
ID
E
EYE

Figure 1.3-3 TI-T-BPH Eyepiece Base Unit
(for External Phase Contrast)

This is an eyepiece tube base for external phase
contrast microscopy.
A turret is provided at the front to attach external
phase plates. Three positions, A to C, are
available. Focus settings can be adjusted for three
positions individually. Besides, phase plates can be
centered with the screws at both side and clamped
with the screw at the back.
A side port with a C-mount adapter is provided at
left. Use the optical path selector knot at right to
change the optical path between the eyepiece side
and the side port side.
Use the side port for photomicroscopy of external
phase contrast microscopy.

Dovetail joint
Eyepiece tube
clamp screw
Centering adjustment
screws (2 places)
Focus adjustment screw
Eyepiece tube
clamp screw
Optical path
selector knob
Centering
clamp screws
(rear, 2 places)
Dovetail joint
Eyepiece tube
clamp screw
Side port
(with C-mount adapter)
Optical path
selector knob
Stage mount
Eyepiece tube
mount
Microscope attachment
side (bottom)
Stage mount
Eyepiece tube
mount
side (bottom)
Stage mount Eyepiece tube mount
Side port
(with C-mount adapter)
side (bottom)
Turret
Dovetail joint
18
TI-TS Eyepiece Tube B

Figure 1.3-4 TI-TS Eyepiece Tube B

This is a basic type eyepiece tube.

TI-TD Eyepiece Tube B
B

Figure 1.3-5 TI-TD Eyepiece Tube B

This is an eyepiece tube with a manual shutter and a
Bertrand lens.
The optical path for the binocular part can be
blocked with the shutter for photomicrography.
Besides, the objective pupil can be observed when
the Bertrand lens is placed in the optical path.

TI-TERG Ergonomic Eyepiece Tube
B

Figure 1.3-6 TI-TERG Ergonomic Eyepiece Tube

This is an eyepiece tube with a tilt mechanism for
user's physical attribute.
This eyepiece tube has a manual shutter and a
Bertrand lens. The optical path for the binocular part
can be blocked with the shutter for
photomicrography. Besides, the objective pupil can
be observed when the Bertrand lens is placed in the
optical path.

Eyepiece
(both sides,
sold separately)
Binocular part
Eyepiece
(both sides,
sold separately)
Binocular part
Shutter
open/close lever

Bertrand lens
focus knob
Bertrand lens
in/out lever
Eyepiece
(both sides,
sold separately)
Binocular part
Shutter
open/close lever

Bertrand lens
focus knob
Bertrand lens
in/out lever
1.4 PFS Motorized Nosepiece and PFS Offset Controller
19
The PFS Motorized Nosepiece (TI-ND6-PFS Perfect Focus Unit) is an integrated combination of a
motorized sextuple DIC nosepiece and a perfect focus system (PFS). The offset can be controlled with the
PFS Offset Controller.

PFS Motorized Nosepiece

OUT
DICHROIC MIRROR
IN

Figure 1.4-1 PFS Motorized Nosepiece

P
F
S
D
O
W
N
U
P
P
F
S
O
F
F
S
E
T
C
O
N
T
R
O
L
L
E
R
F
I
N
E
C
O
A
R
S
E

Figure 1.4-2 PFS Offset Controller

* The offset controller body is magnetically attached to the base plate.
Use caution when lifting PFS Offset Controller, as the base plate may
become detached and fall.

DICHROIC MIRROR - IN/OUT lever
(dichroic mirror operation lever)
Optical component housing
(Do not place items on the box.
Do not apply excessive force.)
Objective sockets (x6)
DIC prism ports (x6)
Interface cable
(Connect to OFFSET connector
on the Ti-E microscope body.)
Base plate
(detachable, magnetic)
FINE/COARSE selector switch
4x and 10x objectives can be
operated in FINE mode only.
The FINE/COARSE selector
switch will be disabled for
these objectives.
Offset dial
Mounting screws
Main body connection cable
(bottom)
1.5 Stage
20
1.5 Stage
The following stages can be attached to the product.

TI-SR Rectangular Mechanical Stage

Figure 1.5-1 Rectangular Mechanical Stage

The specimen can be moved in the X and Y
directions by operating the stage knob.
The rectangular mechanical stage comes with two
stage clips for culture vessels, and the following two
concentric rings.

25 mm bore
(with oiling notch)
40 mm bore

TI-SP Plain Stage

Figure 1.5-2 Plain Stage

Simple, fixed stage for easy operation of specimens.
The plain stage comes with the following two
concentric rings.

25 mm bore
(with oiling notch)
40 mm bore

TI-SAM Attachable Mechanical Stage

Figure 1.5-3 Attachable Mechanical Stage
(on Plain Stage)

This stage is attached to the plain stage when using
specimen holders. The specimen can be moved in
the X and Y directions by operating the stage knob.
Various specimen holders can be used by attaching
the following adapters to the provided microplate.
MA60 microplate holder
MA60 Petri dish holder
MA glass slide holder
35 Petri dish holder

Stage ring
(concentric ring)

Rack

Stage knob
Y-axis direction
X-axis direction
Stage ring
(concentric ring)
Stage knob
Y-axis direction
X-axis direction
Plain stage
Attachable
mechanical stage
1.6 Dia Pillar Illuminator
21
1.6 Dia Pillar Illuminator
Ti series microscopes can be used with either of the following two types of dia pillar illuminators. The two
dia pillar illuminators differ in lamp rating (12 V 100 W or 6 V 30 W) and support different microscopy
methods. Select the dia pillar illuminator to suit your application.
1.6.1 TI-DH Dia Pillar Illuminator 100W
The TI-DH Dia Pillar Illuminator 100W is used with a separate lamphouse and condenser.
The following illustration shows the TI-DH Dia Pillar Illuminator 100W with the D-LH/LC Precentered
Lamphouse and the TI-C Condenser Turret (System Condenser) attached.

Figure 1.6-1 TI-DH Dia Pillar Illuminator 100W
(with lamphouse and system condenser)

1.6.2 TI-DS Dia Pillar Illuminator 30W
The TI-DS Dia Pillar Illuminator 30W has a built-in lamphouse, but requires a separate condenser.
The following illustration shows the TI-DS Dia Pillar Illuminator 30W with the ELWD-S Condenser.

EN
TI - DS 30W
6V
30W
HALOGEN

Figure 1.6-2 TI-DS Dia Pillar Illuminator 30W
(with ELWD-S Condenser)
D-LH/LC Precentered
Lamphouse LC
(Requires TI-PS100W
power supply)
Lamp cable
Condenser mount
(rotatable)
Condenser mount
rotation clamp screw
Condenser
centering knobs
Condenser lens
TI-C Condenser Turret
(system condenser)
Condenser clamp screw
Pillar locking clamp screw
Filter sliders (both sides, 4 total)
Condenser
refocusing clamp
(both sides)
Condenser holder
(detachable)
Condenser holder
clamp screw
Field diaphragm slider
Lamphouse (6 V 30 W lamp sold separately)
(Requires TE-PS30W or TE-PSE30
Power Supply A.)
Filter sliders (3 total)
Dust-proof slider
Device attachment screw holes (M4, x8)
Condenser mount
Condenser holder
ELWD-S Condenser
1.7 Condenser
22
1.7 Condenser
1.7.1 TI-C Condenser Turret (System Condenser)
The TI-C Condenser Turret allows you to attach various optical elements to a turret and select them as
necessary for different microscopy methods. This type of condenser is referred to as a system condenser.

System condenser (mounted on TI-DH Dia Pillar Illuminator 100W)

Figure 1.7-1 TI-C Condenser Turret
(mounted on TI-DH Dia Pillar Illuminator 100W)
* To use a condenser lens of CLWD, oil immersion type for dark-field,
or dry type for dark-field, attach it with the TI-DF condenser adapter.

System condenser (mounted on TI-DS Dia Pillar Illuminator 30W)
The system condenser can be mounted on the TI-DS Dia Pillar Illuminator 30W for HMC observation only.

(mounted on TI-DS Dia Pillar Illuminator 30W)
Condenser focus knob
Condenser focus knob
Condenser centering knobs
Condenser lens
(6 types: ELWD, LWD, HMC, CLWD*,
oil immersion type* for dark-field, and
dry type* for dark-field)
Condenser cassette clamp screws
(both sides of cassette)
Condenser turret
Condenser cassettes
(Ph condenser cassettes have
phase contrast ring centering
screws on the sides.)
Aperture diaphragm
open/close lever
Condenser holder
Condenser lens
(for HMC only)
Aperture diaphragm
open/close lever
Condenser cassette clamp screws
(both sides of cassette)
Condenser turret
Condenser cassettes
Condenser refocusing clamp
1.7 Condenser
23
1.7.2 ELWD-S Condenser

Figure 1.7-3 ELWD-S Condenser

The ELWD-S Condenser supports bright-field
microscopy and phase contrast microscopy. It can
be used with both 100W and 30W dia pillar
illuminators.

1.7.3 High NA Condenser Holder

Figure 1.7-4 High NA condenser holder

The high NA condenser holder is used with the high
NA condenser lens for differential interference
contrast (DIC) microscopy, phase contrast
microscopy, and bright-field microscopy under high
magnification.
The holder can be attached only to the Dia Pillar
Illuminator 100W. And the high NA common
condenser lens is required, too.
The dust-proof plate can be attached also to the
back side by detaching the dust-plate clamp screws
from the original positions and reattaching them to
the opposite side clamp screw holes.

Condenser lens
Turret
Centering knob
Clamp screws
(both sides)
Condenser
centering knobs
(both sides)
Slider lever
Extension tube
(provided with the
high NA common
condenser lens)
Aperture
diaphragm knob
Condenser cassette
clamp screws
Condenser cassette
clamp screws
Dust-proof plate
clamp screws
Dust-proof plate
Front side
Back side
High NA condenser lens
(dry type or oil immersion
type)
Slider clamp
screw
Cassette
slider
1.8 Power Supply
24
1.8 Power Supply
1.8.1 TI-PS100W Power Supply

Warning

The bottom of the power supply device becomes hot during use. Do not block the air vents on the side of
the product.

Figure 1.8-1 TI-PS100W Power Supply

POWER switch
This is the power switch for the power supply. Press
the I side of the switch to turn on the power supply
and output DC power from the 12 VDC output on the
rear. Press the O side of the switch to turn off the
power supply.
POWER indicator
Lit when the power supply is on.
Brightness control knob
When the EXTERNAL switch is turned off, the knob
controls the brightness of the lamp by adjusting the
voltage supplied from the 12 VDC output connector.
AC inlet
This is the connector for connecting the power
supply device to a wall outlet. Be sure to use the
specified power cord for the connection.
EXTERNAL (external control) ON/OFF switch
Turn this switch on to use the brightness control
knob on the microscope for output voltage control.
When this switch is turned off, the brightness control
knob on the front of the power supply becomes
enabled, and the brightness control knob on the
microscope becomes disabled.
EXTERNAL (external control signal input)
connector
Connect the control cable to this
connector and the LAMP CTRL
connector on the rear of the
microscope.
1 2
6
8
7
3 4 5

Pin Signal
1
External resistor terminal for output voltage adjustment
2
3
Output voltage ON/OFF switch (input)
4
GND (0 V)
5
External voltage input for output voltage adjustment
6
EXTERNAL switch on/off detect signal (output)
7
GND (0 V)
8
Output voltage monitor terminal (output)
Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.
12 VDC output connector
This connector supplies power to the 12 V 100 W
halogen lamp. Connect the lamp cable for the pillar
illuminator.
Pin Signal
1 Output +
2 Output -
1 2
3

3 Not used
Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

POWER switch
POWER indicator
Brightness
control knob
AC inlet
12 VDC output
connector
EXTERNAL connector
EXTERNAL ON/OFF switch
1.8 Power Supply
25
1.8.2 TE-PS30W Power Supply A (for 100-120V)
TE-PSE30 Power Supply A (for 230V)

Warning

Before turning on the power supply, check that the input voltage indicator matches the
power voltage in your area. If the voltages do not match, do not turn on the product, and
contact Nikon. Use of the product under a wrong voltage may result in malfunction or fire.
The bottom of the power supply device becomes hot during use. Do not block the air vents
on the side of the product.

Figure 1.8-2 TE-PS30W, TE-PSE30 Power Supply A

POWER switch/indicator
This is the power switch for the power supply. Press
the I side of the switch to turn on the power supply
and output DC power from the 6 VDC output on the
rear. The switch is lit when the power supply is on.
Press the O side of the switch to turn off the power
supply.
When the CTRL switch is turned off, this knob
controls the brightness of the lamp by adjusting the
voltage output from the 6 VDC output connector.
AC inlet
This is the connector for connecting the power
supply device to a wall outlet. Be sure to use the
specified power cord for the connection.
CTRL (external control) ON/OFF switch
Turn this switch on to use the brightness control
knob on the microscope for output voltage control.
When this switch is turned off, the brightness control
knob on the front of the power supply becomes
enabled, and the brightness control knob on the
microscope becomes disabled.

CTRL (external control signal input) connector
Connect the control cable to this
connector and the LAMP CTRL
connector on the rear of the
microscope.
1 2
6
8
7
3 4 5

Pin Signal
1
2
3
Output voltage ON/OFF switch (input)
4
GND (0 V)
5
Not used
6
Not used
7
Not used
8
Not used
Connector: HR12-10R-8SC by Hirose Electric Co., Ltd.
This connector supplies power to the 6 V 30 W
halogen lamp. Connect the lamp cable for the pillar
illuminator.
Pin Signal
1
Output -
2
Not used
1 2
3

3
Output +
Connector: SRCN2A13-3S by Japan Aviation Electronics Industry, Ltd.

Brightness
control knob
AC inlet
CTRL connector
CTRL ON/OFF switch
POWER
switch/indicator
1.9 AC Adapter
26
1.9 AC Adapter

Figure 1.9-1 AC adapter

Power to the microscope is supplied via an AC
adapter.
The AC adapter can be used with 100 to 240 VAC at
50-60 Hz, and can be used with most wall outlets in
the world.
Use the AC adapter included with the product.
Use a power cord specified in Chapter 7,
Specifications.

AC inlet
AC adapter
DC output cable

27
Microscopy
2

Warning

Before using the product, thoroughly read the Safety Precautions at the beginning of this
manual, and heed all warnings and cautions written therein.
To use other equipment such as epi-fl attachment or differential interference contrast
attachment, refer to the respective manuals and heed all warnings and cautions written
therein.

Caution

When using the PFS Motorized Nosepiece, use the Ti Control setup software for Ti series to
register the objective information from a PC. PFS Motorized Nosepiece will not function
properly unless the objective information has been registered correctly. For details on using
Ti Control, refer to the Ti Control instruction manual.

When using Ti-E or Ti-E/B with
TI-HUBC/A Hub Controller A attached on the back

Refer to the instruction manual provided with TI-HUBA/B Hub Controller A
The TI-HUBC/A Hub Controller A is used to control the motorized components.
When the TI-HUBC/A Hub Controller A is attached to the back of the microscope, operation
procedures for the motorized devices will change. Refer to the instruction manual for the TI-HUBC/A
Hub Controller A, and prepare and observe accordingly.
Note that the following two operations cannot be performed electrically even if the TI-HUBC/A Hub
Controller A is attached:
6 V 30 W lamp operation: Use the dia illumination lamp ON/OFF switch.
6 V 30 W lamp voltage adjustment: Use the brightness control knob on the microscope body or
on the power supply device.

Chapter 2 Microscopy
2.1 Introduction to Microscopy
28
The Ti series microscopes are system microscopes that offer a high degree of flexibility in system building for
various purposes. A wide range of options is available for various parts, including the main body, dia
illuminator, and eyepiece tube. For this reason, the operation procedures will vary depending on the system
configuration.
In this chapter, the following common configuration is used as an example to explain the microscopy
procedures.
Sample configuration
Ti-E, PFS Motorized Nosepiece, TI-DH Dia Pillar Illuminator 100W, System Condenser,
and TI-TD Eyepiece Tube B
Bright-field microscopy (see 2.2)
Phase contrast microscopy (without external phase contrast devices) (see 2.3)
External phase contrast microscopy (see 2.4)
In-focus observation with PFS (see 2.5)

For the name and position of each part, refer to Chapter 1, Part Names.
For details on operation methods, refer to Chapter 3, Operation.
If the configuration of your microscope system differs from the examples used in this chapter, refer to the
relevant sections of Chapter 3, Operation.
If your microscope system has the epi-fl attachment or the differential interference contrast attachment,
refer to the respective instruction manuals.
If your microscope system is equipped with the TI-HUBC/A Hub Controller A, refer to the instruction
manual provided with the hub controller.
29
Sample configuration
This section describes the microscopy procedures under the assumption that the following devices are
attached to the microscope.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
PFS
D O W N
UP
PFS
OFFSET
CONTROLLER
FINE
COARSE

Figure 2.1-1 Sample configuration

1. Ti-E microscope body
2. AC adapter, power cord *
3. TI-T-B Eyepiece Base Unit
4. TI-TD Eyepiece Tube B
5. CFI 10x eyepiece (x2)
6. TI-DH Dia Pillar Illuminator 100W,
filters (NCB11, ND4, GIF)
7. D-LH/LC Precentered Lamphouse LC,
12 V 100 W Halogen Lamp
8. TI-PS100W Power Supply, power cord
9. TI-C Condenser Turret,
LWD Condenser Lens,
condenser cassette for bright-field
microscopy,
condenser cassette for phase contrast
microscopy
10. TI-SR Rectangular Mechanical Stage
11. TI-ND6-PFS PFS Motorized Nosepiece,
PFS Offset Controller,
10x and 40x objectives,
phase contrast objective
* 2. AC adapter, power cord not shown in Figure 2.1-1.

9
3
4
5
6
7
8
10
11
1
11
2.2 Bright-Field (BF) Microscopy
30
Bright-field (BF) microscopy workflow
Outline: Remove all unnecessary optical elements from the optical path. Focus and center the
condenser. Adjust the aperture diaphragm for a better image.

1
Reset convenient functions. Page 31

2
Turn on the dia illuminator. Page 31

3
Adjust the illumination for optimal color reproduction. Page 32

4
Prepare the optical path. Page 32

5
Set up for bright-field microscopy. Page 33

7
Adjust the diopters and the interpupillary distance. Page 34

8
Re-adjust the focus. Page 34

9
Center the condenser. Page 35

10
Observe the specimen. Page 36

11
Change the specimen. Page 37

12
End the observation. Page 37

31
1

Reset convenient functions.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.2-1 Resetting convenient functions

1. Release the condenser refocusing clamp on
the dia pillar illuminator by rotating it
counterclockwise.
2. Open the shutter at the eyepiece by pushing
in the shutter operation lever on the right
side of the eyepiece tube.
3. Move the Bertrand lens out of the optical path
by moving the Bertrand lens operation lever on
the front of the eyepiece tube to position O.
4. Rotate the intermediate magnification
selector dial on the front of the microscope
to the 1x side.

2

Turn on the dia illuminator.

Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10
L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS

Figure 2.2-2 Turning on the dia illuminator

1. Set the EXTERNAL switch on the back of the
power supply to ON.
2. Turn on the power supply by pressing the I
side of the POWER switch on the power supply.
3. Turn on the Ti-E microscope by pressing the
ON side of the POWER switch on the back
of the microscope.
The Ti-E microscope is turned on, and the
current status is displayed on the status display
panel.
4 Turn on the lamp by pressing the dia
illumination lamp ON/OFF switch on the left
side of the microscope.
The display content of the status display panel
can be switched between the following three
screens by pressing the DISPLAY switch. Switch
the screen as necessary to display the
information required for the operation.
Display content of the status display panel
Z:____0.000um
E100_Coarse

Upper: Z-axis position
Lower: Optical path | Focus knob resolution
____________10x/0.25
E100_Coarse__PFS:Out

Upper: Objective data
Lower: Optical path | Focus knob resolution | PFS
___10x_Z:____0.000um

Upper: Magnification | Z-axis position
Lower: Optical path | Focus knob resolution | PFS
* Additional display patterns will become available when
using motorized option devices. For details of the display
content, refer to the instruction manual provided with Hub
Controller A.

1-1
1-2
1-3
1-4
2-2
2-1
2-4
2-3
32
3

Adjust the illumination for optimal color reproduction.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 2.2-3 Adjusting the illumination

1. Rotate the brightness control knob on the left
side of the microscope to the 12 V 100 W
position.
2. Move the NCB11 filter on the dia pillar
illuminator into the optical path.
3. Move the ND4 filter on the dia pillar
illuminator into the optical path.

4

Prepare the optical path.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 2.2-4 Setting the optical path

1. Move the 10x objective into the optical path
by pressing the Obj. switch on the left
operation panel.
The status display panel can be used to confirm
which objective is in the optical path.
Display example for objective (10x, NA 0.25)
____________10x/0.25
2. Direct a 100% light towards the eyepiece
observation port by pressing the EYE switch
on the front operation panel.
The pressed switch will light up in green, and the
port name E100 will be displayed on the status
display panel.
Display example for output port
Z:____0.000um
E100_Coarse
3. Fully open the aperture diaphragm by
rotating the aperture diaphragm knob on the
dia pillar illuminator clockwise to the limit.
4. Fully open the aperture diaphragm by moving
the aperture diaphragm open/close lever on
the system condenser to the limit.
5. Lower the condenser mount to the limit by
rotating the condenser focus knob on the dia
pillar illuminator.
If an ELWD condenser lens is attached to the
system condenser, place the condenser mount
1 cm below the upper limit.
If using the ELWD-S condenser, position the
condenser mount approximately 2 cm below
the upper limit.

3-1
3-2, 3-3
4-3
4-5
4-1
4-2
4-4
33
5

Set up for bright-field microscopy.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.2-5
Selecting the condenser cassette for bright-field microscopy

1. Move the condenser cassette for bright-field
microscopy into the optical path by rotating
the condenser turret to position A.

6

Set specimen and adjust the focus.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.2-6
Setting the specimen and focusing on it

1. Place the specimen onto the stage.
2. Move the stage to bring the observation
target into the center of the field of view.
3. Look into the eyepiece. Adjust the focus onto
the specimen by using the Coarse/Fine/
ExFine switches and the focus knobs on the
sides of the microscope.
Use the Coarse/Fine/ExFine switches to change
the focus knob resolution (speed of vertical
movement) for easier focus adjustment. There
are three resolutions: Coarse, Fine, and ExFine
(extra fine). The current setting will be displayed
by an indicator.
Focus knob resolution and Z-axis position can be
displayed on the status display panel.
Display example for focus knob resolution
and Z-axis position
Z:_ 124.225um
E100_Fine

The Z-axis position information on the status
display panel can be reset to zero by pressing the
Z-RESET switch on the front operation panel.
By pressing the Z-RESET switch when the focus
is on the specimen, you will be able to use the
Z-axis position value as reference when
readjusting the focus.

5-1
6-2
6-3
6-1
6-3
34
7

Adjust the diopters and the interpupillary distance.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Diopter adjustment on eyepieces

Interpupillary adjustment on binocular

Figure 2.2-7
Diopter adjustment and interpupillary distance adjustment

1. On each eyepiece, rotate the diopter
adjustment ring to align its lower end with the
marking on the eyepiece.
This will be the reference position for diopter
adjustment.
by pressing the Obj. switch.
3. Look into the left eyepiece with your left eye.
Adjust the focus onto the specimen by
rotating the focus knobs.
the focus knob resolution for easier focus
adjustment.
rotating the diopter adjustment ring on the
left eyepiece.
Do not touch the focus knobs at this time.
6. Repeat steps 2 through 5 two times.
7. Adjust the right eyepiece.
Repeat steps 2 through 6, but this time using the
right eyepiece instead of the left.
8. Adjust the interpupillary distance of the
binocular part to merge the two fields of view.

8

Re-adjust the focus.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.2-8 Focusing on the specimen again

1. Look into the eyepiece. Move the stage to
bring the observation target into the center of
the field of view.
2. Focus on the target by rotating the focus
knobs.
the focus knob resolution for easier focus
adjustment.

Marking
Adjust interpupillary
distance of binocular part
to merge fields of view.
7-1, 7-5,
7-8
7-2, 7-4
7-3
8-1
8-2
8-2
Diopter
adjustment ring
35
9

Center the condenser.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Field diaphragm adjustment

Move field diaphragm image into center of field of
view. Adjust its size to match field of view.
Figure 2.2-9 Centering the condenser

1. Check that the 10x objective is in the optical
path.
The status display panel can be used to confirm
which objective is in the optical path.
Display example for objective (10x, NA 0.25)
____________10x/0.25
If not, move the 10x objective into the optical
path by pressing the Obj. switch on the left side
of the microscope.
2. Rotate the field diaphragm knob on the dia
pillar illuminator until the field diaphragm
image is visible in the field of view.
3. Adjust the focus onto the field diaphragm
image by rotating the condenser focus knob
on the dia pillar illuminator.
4. Move the field diaphragm image to the center
of the field of view by turning the two
condenser centering screws on the dia pillar
illuminator.
6. Adjust the size of the field diaphragm image
to closely match the size of the field of view,
by rotating the field diaphragm knob on the
dia pillar illuminator.
of the field of view by turning the two
condenser centering screws on the dia pillar
illuminator.

9-1
9-2, 9-6
9-4, 9-7
9-3
(9-1), 9-5
Field diaphragm image
Field of view
36
10

Observe the specimen.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Aperture diaphragm adjustment
70~80
100

Adjust the size of the aperture diaphragm image to
70-80% the size of the objective pupil plane.
Figure 2.2-10 Observing the specimen

1. Move an objective with the desired
magnification into the optical path by
pressing the Obj. switch.
2. Adjust the size of the aperture diaphragm to
70-80% the size of the NA of the objective
by moving the aperture diaphragm
open/close lever on the system condenser.
Move the Bertrand lens into the optical path by
moving the Bertrand lens in/out lever on the
eyepiece tube to position B. This will allow you
to observe the objective pupil plane and the
aperture diaphragm image. Adjust the focus by
rotating the Bertrand lens focusing knob on the
right side of the eyepiece tube, and then adjust
the size of the aperture diaphragm image to
70-80% the size of the objective pupil plane.
When done, move the Bertrand lens out of the
optical path by moving the operation lever to
position O.
4. Adjust the brightness for the field of view by
moving the ND filters on the dia pillar
illuminator in and out of the optical path.
If color reproducibility is not required, brightness
adjustment can be performed by changing the
lamp voltage with the brightness control knob on
the left side of the microscope.
Adjust the focus by rotating the focus knobs.

10-3
10-4
10-1
10-2
(10-2)
10-5
10-5
Objective pupil plane
Aperture diaphragm image

37
11

Change the specimen.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.2-11 Changing the specimen

Use the following functions as necessary.
Inclining of dia pillar illuminator
When using the 100W dia pillar illuminator, the
entire dia pillar illuminator can be inclined
backward by loosening the fixing knob on its
back side, so as to secure more working space.
Escape switch, Refocus switch
If there is a need to lower the objective, the
objective can be retracted temporarily by
pressing the Escape switch on the right
operation panel.
To return the objective to approximately the
same height, press the Refocus switch without
If there is a need to move the condenser up and
down, tighten the condenser refocusing clamp
before moving the condenser. This will make it
easier to restore the condenser to the original
position.

12

End the observation.

Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10
L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS

Figure 2.2-12 Ending the observation

1. Turn off the dia illumination by pressing the
dia illumination lamp ON/OFF switch on the
left side of the microscope.
2. Turn off the microscope by pressing the
OFF side of the POWER switch on the back
of the microscope.
3. Turn off the power supply by pressing the
O side of the POWER switch on the back of
the power supply.
If placing a cover on the microscope, wait until
the lamp has cooled sufficiently.

12-3
12-1
12-2
Condenser refocusing
clamp
Escape switch,
Refocus switch
Inclining of
dia pillar
illuminator
2.3 Phase Contrast (Ph) Microscopy
38
Phase contrast (Ph) microscopy workflow
Outline: Place a phase contrast objective and a condenser cassette that have the same Ph code
into the optical path. Center the position of the condenser annular diaphragm in the
condenser cassette so that it is aligned with the phase plate ring in the objective.
1
Adjust the focus onto the specimen with BF microscopy. Page 39

2
Set up for phase contrast microscopy. Page 39

3
Center the condenser annular diaphragm. Page 40

4

5
Change the objective. Page 41

6

7

39
1

Adjust the focus onto the specimen with BF microscopy.

For the BF microscopy procedure, refer to Section
2.2 Bright-Field (BF) Microscopy.

2

Set up for phase contrast microscopy.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 2.3-1
Setting up for phase contrast microscopy

1. Move the phase contrast objective into the
optical path by pressing the Obj. switch on
the left operation panel.
Check the Ph code of the objective.
2. Rotate the condenser turret to the position
for the Ph code of the objective.
3. Fully open the aperture diaphragm by moving
the aperture diaphragm open/close lever on
the condenser clockwise to the limit.
If the aperture diaphragm is not fully open, the
optical path of the annular diaphragm will overlap
with the aperture diaphragm, and the phase
contrast effect cannot be achieved.
Be sure to fully open the aperture diaphragm
when performing phase contrast microscopy.
4. Fully open the aperture diaphragm by
rotating the aperture diaphragm knob on the
dia pillar illuminator clockwise to the limit.

When using the ELWD-S condenser

Fig. 2.3-2 ELWD-S condenser
The aperture diaphragm of the ELWD-S condenser
is used only for bright-field microscopy. It does not
need to by fully opened in step 3, as it has no effect
on the optical path for phase contrast.
The ELWD-S condenser is designed so that all
annular diaphragms become centered when the
annular diaphragm is centered at the PhL position.
Move the PhL objective into the optical path,
rotate the condenser turret to the PhL position,
and proceed to the next step.

2-4
2-1
2-2, 2-3
40
3

Center the condenser annular diaphragm.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Centering the annular diaphragm

Fig. 2.3-3 Centering the annular diaphragm

1. Move the Bertrand lens into the optical path
by moving the Bertrand lens in/out lever to
position B. Adjust the focus onto the
annular diaphragm image by rotating the
Bertrand lens focusing knob on the right side
of the eyepiece tube.
2. Using the provided hex screwdriver, rotate
the two annular diaphragm centering screws
on the condenser cassette, and adjust the
position of the annular diaphragm image so
that it overlaps with the phase contrast ring
image within the objective.
3. Move the Bertrand lens in/out lever back to
position O.

4


L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.3-4 Phase contrast microscopy

1. Check that the aperture diaphragm of the
condenser is fully open.
illuminator out of the optical path, and move
the GIF filter into the optical path.
The GIF filter improves the contrast for phase
lamp voltage with the brightness control knob on
the left side of the microscope.

3-1, 3-3
3-2
Overlap annular diaphragm image
onto phase contrast ring image within objective.
Annular
diaphragm
image
(4-4)
4-3, 4-4
4-2
4-1
4-5
Phase contrast
ring image
41
5

Change the objective.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 2.3-5 Changing objectives

1. Move a phase contrast objective with the
desired magnification into the optical path by
pressing the Obj. switch.
3. Center the annular diaphragm that is now in
the optical path.
For the centering procedure, refer to step 3 on
page 40.
When attaching phase contrast cassettes to the
system condenser, you will need to center all
phase contrast cassettes.
When using the ELWD-S condenser, all annular
diaphragms can be centered simultaneously by
centering the annular diaphragm at the PhL
position.

6


B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS


operation panel.
position.

5-1
5-2, 5-3
Condenser
refocusing clamp
Escape switch,
Refocus switch
Inclining of
dia pillar
illuminator
42
7


Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10
L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS

Figure 2.3-7 Ending phase contrast microscopy

of the microscope.
the power supply.

7-3
7-1
7-2
2.4 External Phase Contrast Microscopy
43
External phase contrast microscopy is a microscopy method proposed by Nikon to be a new standard
function of inverted research microscopes.
Traditionally, phase contrast microscopy required the use of special objectives with a built-in phase ring (See
Figure 2.4-1). The phase ring is coated with a phase film, as well as an ND film (light reduction film) for
reducing the amount of zero-order light (direct light) that passes. These films sometimes affect imaging and
laser transmission, making the phase contrast objectives difficult to use for other applications (i.e. optical
tweezers and total reflection fluorescence microscopy).

Figure 2.4-1 Optical path for traditional phase contrast microscopy
An external phase contrast system takes an image on the primary image plane, and refocuses it on the
secondary image plane via relay optics. The adoption of such an optical system allows the phase ring to be
placed midway in the relay optics, instead of in the objective. In other words, the phase ring can be moved
out of the objective (See Figure 2.4-2). As a result, phase contrast microscopy can now be performed with
normal objectives (without a phase ring), and phase contrast objectives are no longer required.

Figure 2.4-2 Optical path for external phase contrast microscopy
A camera port is also provided on the external phase contrast optics system, enabling photomicroscopy of
phase contrast images. For example, a primary image plane camera port (i.e. side port of an inverted
microscope) can be use to capture bright fluorescent images unaffected by the phase ring, while by
switching the optical path, the camera port on the external phase contrast eyepiece base unit can be used to
capture phase contrast images.
The external phase contrast system also supports high-NA TIRF (total internal reflection fluorescence)
objectives such as Apo TIRF 60x H/1.49 and Apo TIRF 100x H/1.49, allowing TIRF objectives to be fully
utilized for applications that require bright fluorescence images (i.e. monomolecular fluorescence
microscopy), while also allowing morphological observations to be performed without changing the objective.
Annular diaphragm
Condenser
Specimen
Objective Secondary
objective
Primary image plane
Phase ring
Secondary
image plane
Secondary image
plane (camera port)
Epi-fl, TIRF illumination,
optical tweezers, etc.
Relay optics
Annular diaphragm
Condenser
Specimen
Objective Secondary
objective
Phase ring
Epi-fl, TIRF illumination,
optical tweezers, etc.
Image plane
44

Selecting objectives and phase plates
To perform external phase contrast microscopy, you will need to use the TI-T-BPH External Phase Contrast
Eyepiece Base Unit.
You will also need to select a phase plate that is suitable for the objective being used, and attach it to the
eyepiece base unit. Refer to the following table to select an objective and an appropriate phase plate.
Up to three phase contrast plates (A, B, and C) can be attached to the TI-T-BPH External Phase Contrast
Eyepiece Base Unit.
Table 2.4-1 Objectives and phase plates

Condenser phase contrast code
(Supported condenser lens)
Objective
External phase
contrast ring
1 Ph3 (LWD, CLWD) P Apo 60x WI (NA1.2) 60x/Ph3
2 Ph3 (LWD, CLWD) P Apo VC 60x WI (NA1.2) 60x/Ph3
3 Ph3 (LWD, CLWD) P Apo VC 60x H (NA1.4) 60x/Ph3
4 Ph4 (CLWD) Apo TIRF 60x H (NA1.49) 60x/Ph4
5 Ph4 (CLWD) P Apo TIRF 60x H (NA1.45) 60x/Ph4

External phase contrast microscopy workflow
Outline: Place the objective, the condenser lens, and the condenser cassette into the optical
path as a combination shown in the above table. Center the positions of the phase
contrast ring in the eyepiece base unit for external phase contrast microscopy
(TI-T-BPH) and the ring in the condenser cassette.
1

Attach the centering tool for external phase contrast
microscopy to the nosepiece.
Page 45

2
Focus on the specimen with bright-field microscopy. Page 45

3
Center the external phase contrast ring. Page 45

4
Center the condenser annular diaphragm. Page 47

5

6
Change the objective. Page 48

7

8

45

1

Attach the centering tool for external phase contrast microscopy to the nosepiece.

Figure 2.4-3
Centering tool for external phase contrast microscopy

When a phase contrast objective is used, the
external phase contrast can be adjusted with
reference to the phase contrast ring of the phase
contrast objective as well.

2

Focus on the specimen with bright-field microscopy.

For the bright-field microscopy procedure, refer to
Section 2.2 Bright-Field (BF) Microscopy

3

Center the external phase contrast ring.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
SIDE
EYE

1. Move the centering tool for external phase
contrast microscopy into the optical path by
pressing the Obj. switch on the left operation
panel.
2. Move the Bertrand lens into the optical path
by moving the Bertrand lens operation lever
on the front of the eyepiece tube to position
B. Focus on the ring image of the centering
tool for external phase contrast microscopy
placed into the optical path, by rotating the
Bertrand lens focusing knob.
The image position of the annular diaphragm will
shift depending on the filter block to be used.
Therefore, if a filter block is necessary for phase
contrast microscopy, place the filter block into the
optical path before the adjustment.
3. Rotate the eyepiece base unit turret to
place the external phase contrast ring of
the largest Ph code into the optical path.
If multiple rings have the same largest Ph
code, use the ring that has the higher
magnification.
4. Insert the provided hex screwdriver into the
focus adjustment screw hole on the front of
the turret and adjust the screw to focus on
the phase contrast ring.
3-1
3-2
3-2
3-3
3-4
46
Centering the external phase contrast ring
Figure 2.4-4
Centering the external phase contrast ring

centering screw hole on the front of the
eyepiece base unit, and adjust the external
phase contrast ring so that its image is
concentrically aligned to the ring image of
the centering tool.
After adjusting, tighten the two centering clamp
screws on the back of the eyepiece base unit.
When centering with the eyepiece base unit
turret, the whole turret will be moved, unlike
when adjusting the condenser turret. Center the
turret with the external phase contrast ring of the
largest Ph code (and the higher magnification if
multiple rings have the same largest Ph code).
Use the annular diaphragm on the condenser to
center each ring.
However, in the cases below, the eyepiece base
unit turret must be centered each time when
objectives are changed. Be aware of it.
(1) The LWD condenser lens is used.
(2) Objectives of Ph4 ready are changed and
used successively.

Move external phase contrast ring image into
center of field of view.
Phase contrast ring
image
Field of view
Phase contrast ring
47

4

Center the condenser annular diaphragm.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
SIDE
EYE

Centering the annular diaphragm
Fig. 2.4-5 Centering the annular diaphragm

1. Move an external phase contrast objective
into the optical path by pressing the Obj.
switch on the left operation panel.
2. Rotate the condenser turret to place the
annular diaphragm compliant with the
objective into the optical path. Fully open the
aperture diaphragm by rotating the aperture
diaphragm operation lever clockwise to the
limit.
If the aperture diaphragm is not fully open, the
optical path of the annular diaphragm will overlap
with the aperture diaphragm, and the phase
contrast effect cannot be achieved.
3. Focus on the annular diaphragm by rotating
the Bertrand lens focusing knob on the
eyepiece tube.
4. Rotate the eyepiece base unit turret to place
the phase contrast ring that is suitable for the
objective in the optical path.
focus adjustment screw hole on the front of
the turret and adjust the screw to focus on
the phase contrast ring.
6. Use the provided hex screwdriver to rotate
the two annular diaphragm centering screws
on the condenser cassette, and adjust the
annular diaphragm position so that its image
is aligned to the external phase contrast ring
image.
Check that the two images are concentric.
7. Move the Bertrand lens out of the optical
path by moving the Bertrand lens in/out lever
back to position O.

4-3
4-2
Align the annular diaphragm image
with the external phase contrast ring image.
Annular diaphragm
image
External phase
contrast ring image
4-7
4-4
4-5
4-6
48

5


L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
SIDE
EYE

Figure 2.4-6 External phase contrast microscopy

illuminator out of the optical path, and move
the GIF filter into the optical path.
The GIF filter improves the contrast for phase
lamp voltage with the dia illumination brightness
control knob on the left side of the microscope.

To photograph observation images, attach a
C-mount type camera to the side port of the
eyepiece base unit.

6

Change the objective.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 2.4-7 Changing objectives

1. Move another external phase contrast
objective into the optical path by pressing the
Obj. switch on the left operation panel.
2. Rotate the eyepiece base unit turret to the
position for the external phase contrast ring
that is suitable for the objective in the optical
path.
4. Center the annular diaphragm of the
condenser.
For the centering procedure, refer to step 4 on
page 47.

6-1
6-3, 6-4
6-2
5-2, 5-3
(5-3)
5-1
5-4
49

7


B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
SIDE
EYE


operation panel.
position.

8


L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10

Figure 2.4-9
Ending external phase contrast microscopy

of the microscope.
the power supply.

8-3
8-1
8-2
Condenser
refocusing clamp
Escape switch,
Refocus switch
Inclining of
dia pillar
illuminator
2.5 In-focus Observation with PFS
50
In-focus observation is an observation method implemented by combining a Ti-E or Ti-E/B microscope with a
PFS Motorized Nosepiece. The system detects the boundary surface between the cover glass and the
aqueous solution of the specimen (for water immersion or oil immersion objectives)
(NOTE 1)
or between the
cover glass and air (for dry objectives)
(NOTE 1)
, and maintains the focus by controlling the focusing
mechanism to track the vertical movement of the boundary surface. This method achieves a constant focal
distance by compensating for minor focus shifts that may result over time or by stage movements. In this
manual, this method will be referred to as in-focus observation (or PFS observation
(NOTE 2)
).
NOTE 1: The boundary surface is also referred to as an interface.
NOTE 2: PFS = Perfect Focus System
In-focus observation workflow
Outline: Perform bright-field microscopy or phase contrast microscopy with the TI-ND6-PFS PFS
Motorized Nosepiece.
* To perform in-focus observation, you must register the objective information on the microscope.
For registration details, refer to Chapter 4, Assembly. This section describes the microscopy
procedures under the assumption that the objective information has already been registered.
1

Focus on the specimen with bright-field microscopy or
phase contrast microscopy.
Page 51

2
Set up the perfect focus system. Page 51

3
Start the operation of the perfect focus system. Page 52

4

5
End the operation of the perfect focus system. Page 53

6

51
1

Focus on the specimen with bright-field microscopy or phase contrast microscopy.

Set up for bright-field microscopy or phase contrast
microscopy, as described in Section 2.2,
Bright-Field (BF) Microscopy, Section 2.3, Phase
Contrast (Ph) Microscopy, or Section 2.4, External
Phase Contrast Microscopy.

2

Set up the perfect focus system.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 2.5-1 Setting up for PFS observation

1. Move a PFS objective with the desired
magnification into the optical path by
pressing the Obj. switch on the left operation
panel.
2. Attach an IR filter (infrared filter) to the filter
sliders on the pillar illuminator, and move it
into the optical path.
PFS uses near-infrared light for focus
management. Be sure to place the IR filter into
the optical path to minimize the effect of the heat
rays (infrared light) emitted by the illumination.
3. Display the PFS information on the status
display panel by pressing the DISPLAY
switch on the front operation panel.
At this point, PFS: Out will be displayed.
Display example for PFS information
____________10x/0.25

4. Move the dichroic mirror into the optical path
by moving the DICHROIC MIRROR - IN/OUT
lever on the top of the PFS Motorized
Nosepiece to the IN position.
The PFS information display will change to PFS:
Off.
Display example for PFS information
____________10x/0.25
E100_Coarse__PFS:Off
If PFS: ER1 is displayed, the objective has not
been configured properly. Check and register the
objective. Refer to the list of supported
objectives on page 128, and to Chapter 4,
Assembly.

2-1
2-2
2-3
2-4
52
3

Start the operation of the perfect focus system.

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse Fine ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1
.5
X

Figure 2.5-2 Starting up the PFS operation

2. Check that the FOCUS indicator on the front
operation panel is lit.
If not, try moving the focus slightly.
3. Start the PFS function by pressing the
PFS-ON switch on the front operation panel.
The PFS-ON switch will be lit, and the PFS
information will change to PFS: On.
PFS control started
____________10x/0.25
E100_Coarse__PFS:On

4


P
F
S
D
O
W
N
U
P
P
F
S
O
F
F
S
E
T
C
O
N
T
R
O
L
L
E
R
F
IN
E
C
O
A
R
S
E

Figure 2.5-3 Offset adjustment

1. The observation surface can be moved up
and down without losing the focus, by
rotating the offset dial on the PFS Offset
Controller.
The offset traveling distance for the dial can be
switched between Fine and Coarse with the
FINE/COARSE switch on the side of the PFS
Offset Controller.
4x and 10x objectives can be operated in FINE
mode only. The FINE/COARSE selector switch
will be disabled for these objectives.

3-1
3-2, 3-3
(4-1)
4-1
3-2
3-3
3-1
53
5

End the operation of the perfect focus system.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1
.5
X

Figure 2.5-4 Ending the PFS operation

1. Turn off the PFS function by pressing the
illuminated PFS-ON switch.
Off.
PFS control stopped
____________10x/0.25
E100_Coarse__PFS:Off

2. Move the dichroic mirror out of the optical
path by moving the DICHROIC MIRROR -
IN/OUT lever on the top of the PFS Motorized
Nosepiece to the OUT position.
Out.
Dichroic mirror: Out
____________10x/0.25

Be sure to stop the PFS function before changing
the specimen or ending the observation.

6


Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10
L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS

Figure 2.5-5 Ending the observation

of the microscope.
the power supply.

5-2
5-1
5-1
6-3
6-1
6-2

54
Operation
3

Warning

Before using the product, thoroughly read the Safety Precautions at the beginning of this
manual, and heed all warnings and cautions written therein.
To use other equipment such as epi-fl attachment or differential interference contrast
attachment, refer to the respective manuals and heed all warnings and cautions written
therein.

Caution

When using the PFS Motorized Nosepiece, use the Ti Control setup software for Ti series to
register the objective information from a PC. PFS Motorized Nosepiece will not function
properly unless the objective information has been registered correctly. For details on using
Ti Control, refer to the Ti Control instruction manual.

This chapter describes the operation methods for each part of the product.
For the name and position of each part, refer to Chapter 1, Part Names.
For the microscopy procedure, refer to Chapter 2, Microscopy.
If your microscope system has the epi-fl attachment or the differential interference contrast attachment,
refer to the respective instruction manuals.
If your microscope system is equipped with the TI-HUBC/A Hub Controller A, refer to the instruction
manual provided with the hub controller.
Chapter 3 Operation
3.1 Power On/Off
55
3.1 Power On/Off
3.1.1 Power On
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 3.1-1 Power switch locations

Turn on the dia illumination power supply and the
microscope, as described below.
1. Check that the power supply and the
microscope are connected correctly.
For details on the connection, refer to Chapter 4,
Assembly.
2. Check the setting of the EXTERNAL switch
(or the CTRL switch) on the back of the
power supply.
To use the brightness control knob on the
microscope, set the switch to ON. To use the
brightness control knob on the power supply, set
the switch to OFF.
For details, refer to Section 3.2.2, Power Supply
Setting.
3. Turn on the power supply by pressing the I
side of the POWER switch on the front of the
power supply.
When the power is ON, the POWER indicator
will be lit.
* On TE-PS30W and TE-PSE30, the POWER
switch has a built-in power indicator.
MIC CONTROL
POWER
OFF ON
LAMP CTRL
DC24V IN
USB
5 3 1 0 0 1
(2)

Figure 3.1-2 Ti-E (rear view)

4. Turn on the microscope by pressing the
ON side of the POWER switch on the back
of the microscope.
Power to the Ti-E or Ti-E/B microscope is
supplied via the AC adapter. Check the
connection of the AC adapter before turning
on the power.
See Chapter 4, Assembly.
When the power is turned on, indicator lamps on
the front and the side of the microscope will be
lit, and the system status is displayed on the
status display panel on the front.
3.1.2 Power Off

To turn the microscope off, first turn off the
microscope, and then turn off the dia illumination
power supply.
The microscope can be turned off by pressing the
OFF side of the POWER switch on the back of the
microscope body. The power supply can be turned
off by pressing the O side of the POWER switch on
the front of the power supply.
POWER
switch
(back side)
POWER
switch
POWER switch DC24V IN connector
(for AC adapter)
Chapter 3 Operation
3.2 Dia Illumination Operation
56
3.2.1 Combination of Lamp, Dia Pillar Illuminator, and Power Supply
The combination of the dia pillar illuminator and the power supply will differ depending on the rating of the
lamp used (12 V 100 W or 6 V 30 W). Refer to the following table for the correct combination of lamp, dia
pillar illuminator, and power supply. Do not use the devices in any other combination.
Lamp rating Dia pillar illuminator Power supply
12 V 100 W halogen lamp
(OSRAM HLX 64623
or PHILIPS 77241)
TI-DH Dia Pillar Illuminator 100W

TI-PS100W Power Supply
(for 100-240 V)

TE-PS30W
Power Supply A
(for 100-120 V)
TE-PSE30
Power Supply A
(for 230 V)
6 V 30 W halogen lamp
(PHILIPS 5761)
TI-DS Dia Pillar Illuminator 30W
EN
TI - DS 30W
6V 30W
HALOGEN

* Both power supply units have the same appearance.

3.2.2 Power Supply Setting

ON
OFF
E
X
T
E
R
N
A
L
MADE IN JAPAN MODEL TI-PS100W

Figure 3.2-1 Power supply (rear view)

The EXTERNAL switch (or CTRL switch) on the
back of the power supply can be set to allow
switching dia illumination lamp on/off and controlling
brightness from the left operation panel of the
microscope.
If the EXTERNAL/CTRL switch is set to ON, the
operation panel on the microscope will be enabled; if
the switch is set to OFF, the brightness control knob
on the power supply will be enabled. Only one or the
other of the brightness control knobs can be enabled
at a time.
The two knobs are not calibrated. A particular
setting on one knob may result in a different
brightness than for the same setting on the other
knob.

EXTERNAL switch/CTRL switch
ON : Use the brightness control knob
on the microscope.
OFF : Use the brightness control knob
on the power supply device.
Chapter 3 Operation
57
3.2.3 Dia Illumination Lamp Operation

Coarse Fine ExFine
Obj. ON/ OFF
MAX.
12V10
L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
ON/ OFF
6V30W
MAX. MIN.
12V100W

Figure 3.2-2 Dia illumination lamp operation

Switching dia illumination lamp ON/OFF
When the EXTERNAL/CTRL switch on the power
supply is set to ON, the dia illumination can be
turned on/off with the dia illumination ON/OFF switch
on the left operation panel of the microscope.
Push the switch in to turn on the dia illumination.
Push the switch again to turn off the dia illumination.
Adjusting brightness of dia illumination lamp
Two brightness control knobs are provided for the
dia illumination: one on the left side of the
microscope, and the other on the front of the power
supply.
When the brightness control knob is rotated, the
lamp voltage changes, changing the brightness and
the color of the lamp. When the voltage is increased,
the light becomes bright and bluish. When the
voltage is decreased, the light becomes dark and
reddish.
White light
When accurate color reproduction is critical, set the
brightness adjustment knob to the 6 V 30 W or 12
V 100 W position depending on the lamp used, then
move the NCB11 filter into the optical path. This
setting will provide the whitest light. To adjust the
brightness, adjust the ND filters on the dia pillar
illuminator.

3.2.4 Brightness Adjustment with ND Filters

Figure 3.2-3 Brightness adjustment with ND filters

Filters for brightness adjustment are referred to as
ND filters (ND: Neutral Density).
Filters with higher ratings have lower transmittance,
and produces darker images. These filters are useful
for adjusting the brightness when color
reproducibility is critical (i.e. for photomicroscopy), as
they do not affect the color of the light.
Use the ND filters by attaching them to the filter
sliders on the dia pillar illuminator.
ND2: Reduces the light intensity to 1/2.
(50 % transmittance)
(25 % transmittance)
(12.5 % transmittance)
(6.3 % transmittance)
Brightness
control knob
(on power supply)
ON/OFF switch
Filter sliders
Chapter 3 Operation
3.3 Controls on the Microscope Body
58
Ti-E and Ti-E/B microscope bodies have operation panels on the front, left, and right for operation of various
motorized parts.

3.3.1 Front Operation Panel
L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1
.5
X

Figure 3.3-1 Front operation panel

(1) Status display panel
This display shows the microscope status in 2
lines by 20 columns of text. The microscope
status appears here. Multiple display patterns
are provided. Select the pattern that best suits
your need. When the microscope is turned on,
the initial pattern will be displayed. (See page
59.)
(2) DISPLAY switches (display item selection)
The up/down arrow switches change the display
content of the status display panel. Multiple
display patterns are provided. (See page 59.)
(3) BRIGHTNESS switch (display brightness
selection)
This switch changes the illumination of the status
display panel and LEDs on the body between
bright, dim, and off. Use this switch to darken the
room for epi-fl microscopy or so on.
When the switch is pressed, the status will cycle
in the following order: [Panel ON, LED ON]
[Panel dim, LED DIM] [Panel OFF, LED OFF].
(4) Z-RESET switch (Z-axis position reset)
Press this switch to reset the Z-axis position on
the status display panel to zero. The Z-axis
position display will be increased when the
nosepiece is elevated, and decreased when the
nosepiece is lowered. By resetting the Z-axis
position display after setting the focus, you will
be to use the set position as a reference point.
For details, refer to Section 3.10, Focusing
Mechanism Operation.

(5) PFS control switches and indicators
These controls are used for in-focus observation
with the PFS (Perfect Focus System). For
details, refer to Section 3.14, PFS Operation.
FOCUS indicator: The indicator shows the
in-focus status. When the objective is placed in
the focus range, the indicator blinks. When the
PFS focuses on the reference position, the
indicator turns on.
ON switch: This switch turns on/off the in-focus
(PFS) function. The switch body is lit when the
PFS function is ON.
MEMORY switch: Press this switch to register
the distance (offset) from the reference position
to a given focal position. When an offset is
registered, the switch body is lit.
RECALL switch: Press this switch to restore the
offset.
(6) Optical path selector switch/indicator
This switch changes the output port for the
image. The switch for the selected port will be lit.
For details, refer to Section 3.4, Optical Path
Selection.
(7) Intermediate magnification selector knob
(1.5x 1x)
Rotate the knob to change the magnification of
the microscope body between 1x (1x objective
magnification) and 1.5x (1.5x objective
magnification). The setting of this knob is applied
for all output ports.
(1) Status display panel
(5) PFS control switches/indicators
(6) Optical path selector
switch/indicator
(4) Z-RESET switch
(Z-axis position reset)
(3) BRIGHTNESS switch
(display brightness selection)
(2) DISPLAY switches
(display item selection)
(7) Intermediate magnification
selector knob (1.5x 1x)
Chapter 3 Operation
59
Display pattern selection for the status display panel
The display content of the status display panel can be switched between the following three display patterns
by pressing the DISPLAY switch. The display content differs for each display pattern. Select the pattern that
best suits your need.
Pattern Display contents Display example
A
Upper: Z-axis position (unit: m)
Lower: Output port, Focus knob resolution

Z:__124.375um_______
E100_Coarse

B
Upper: Objective status (1)
Lower: Output port, Focus knob resolution, PFS status

P_Fluor____100x/1.30
E100_Coarse_PFS:Off_

C
Upper: Objective status (2), Z-axis position (unit: m)
Lower: Output port, Focus knob resolution, PFS status

PF100x_Z:__124.375um
E100_Coarse_PFS:Off_

* Additional display patterns will become available when using TI-HUBC/A Hub Controller A. For details,
refer to the instruction manual provided with TI-HUBC/A Hub Controller A.

Display content details
Objective status (1)
[name, magnification, NA]
000x/0.00 (Blank) :Achromat
Apo_TIRF 000x/0.00 Apo TIRF :Apo TIRF
Plan 000x/0.00 Plan :Plan
P_Apo 000x/0.00 P Apo :Plan Apo
P_Apo_TIRF 000x/0.00 P Apo TIRF :Plan Apo TIRF
P_Apo_VC 000x/0.00 P Apo VC :Plan Apo VC
P_Fluor 000x/0.00 P Fluor :Plan Fluor
Plan_UW 000x/0.00 Plan UW :Plan UW
S_Fluor 000x/0.00 S Fluor :S Fluor
S_P_Fluor 000x/0.00 S P Fluor :S P Fluor
HMC 000x/0.00 HMC :HMC
Other 000x/0.00 Other :Other information
Objective status (2)
[name, magnification]
000x (Blank) :Achromat
A_000x A :Apo TIRF
P_000x P :Plan
PA000x PA :Plan Apo (TIRF/VC)
PT000x PT :Plan Apo TIRF
PV000x PV :Plan Apo VC
PF000x PF :Plan Fluor
P_000x P :Plan UW
SF000x SF :S Fluor
SP000x SP :S P Fluor
HM000x HM :HMC
OT000x OT :Other information
Output port
E100 Observation port 100%
L80 Left port 80%, observation port 20%
L100 Left port 100%
R80 Right port 80%, observation port 20%
R100 Right port 100%
B100 Bottom port 100%
Z-axis position
Z: 0000.000um + direction: Direction of upward objective movement
Z:-0000.000um - direction: Direction of downward objective movement
Focus knob resolution
Coarse Coarse
Fine Fine
ExFine Extra fine
PFS status
PFS:On PFS function ON
PFS:Off PFS function OFF
PFS:Out Dichroic mirror not in optical path
PFS:DIS Out of PFS function range
PFS:ERn Error (See Chapter 5, Troubleshooting)
Chapter 3 Operation
60
3.3.2 Left Operation Panel
Coarse
Fine
ExFine
Obj.
ON/ OFF
6V30W
MAX. MIN.
12V100W

Figure 3.3-2 Left operation panel

(1) Coarse/Fine/ExFine switch/indicator
(focus knob resolution selector/indicator)
Selects the resolution for the vertical movement
of the nosepiece for when the focus knobs on
the sides are rotated. Press the upper switch to
cycle in the following order: ExFine (extra fine)
Fine Coarse. Press the lower switch to
cycle in the opposite order.
The selected resolution will be indicated by the
indicator on the switch, as well as on the status
display panel.
(2) Focus knob
This knob moves the nosepiece vertically for
focus adjustment. Viewing the microscope from
its left side, a clockwise rotation elevates the
nosepiece, while a counterclockwise rotation
lowers the nosepiece.

(3) Obj. switch (objective selector)
Changes the objective in the optical path by
rotating the PFS Motorized Nosepiece or the
Motorized Sextuple DIC Nosepiece. Press the
front switch for a clockwise rotation, or the rear
switch for a counterclockwise rotation.
TI-HUBC/A Hub Controller A is required to use
the TI-ND6-E Motorized Sextuple DIC
Nosepiece. TI-N6 Sextuple Nosepiece and
TI-ND6 Sextuple Nosepiece are manually
operated, and cannot be controlled by the
Obj. switch.
(4) Dia illumination lamp ON/OFF switch
Turns the dia illumination on/off. Push the switch
in to turn on the dia illumination. Push the switch
again to turn off the dia illumination.
For details, refer to Section 3.2.3, Dia
Illumination Lamp Operation.
(5) Dia illumination lamp brightness control knob
Adjusts the brightness of the dia illumination by
changing the voltage for the lamp. When the
voltage is increased, the light becomes bright
and bluish. When the voltage is decreased, the
light becomes dark and reddish.
When accurate color reproduction is critical, set
the brightness adjustment knob to the 6 V 30 W
or 12 V 100 W position depending on the lamp
used, then move the NCB11 filter into the optical
path.
For details, refer to Section 3.2.3, Dia
Illumination Lamp Operation.

(4) Dia illumination lamp
ON/OFF switch
(1) Coarse/Fine/ExFine
switch/indicator
selector/indicator)
(2) Focus knob
(5) Dia illumination lamp
(3) Obj. switch
(objective selector switch)
Chapter 3 Operation
61
3.3.3 Right Operation Panel
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape

Figure 3.3-3 Right operation panel

(1) Coarse/Fine/ExFine switch/indicator
(focus knob resolution selector/indicator)
Selects the resolution for the vertical movement
of the nosepiece for when the focus knobs on
the sides are rotated. Press the upper switch to
cycle in the following order: ExFine (extra fine)
Fine Coarse. Press the lower switch to
cycle in the opposite order.
The selected resolution will be indicated by the
indicator on the switch, as well as on the status
display panel.
(2) Focus knob
This knob moves the nosepiece vertically for
focus adjustment. Viewing the microscope from
its right side, a clockwise rotation lowers the
nosepiece, while a counterclockwise rotation
elevates the nosepiece.
(3) Epi Shutter switch
(epi illumination shutter operation)
This switch opens/closes the motorized shutter
for the epi illumination.
The Epi Shutter switch becomes enabled
when TI-HUBC/A Hub Controller A and a
motorized shutter for epi illumination are
attached. For details, refer to the instruction
manual provided with Hub Controller A.

(4) FL Block switch (filter block selector)
Changes the filter block in the optical path by
rotating the motorized filter turret.
The FL Block switch becomes enabled when
TI-HUBC/A Hub Controller A and a motorized
filter turret are attached. For details, refer to
the instruction manual provided with Hub
Controller A.
(5) Refocus switch (objective refocusing)
Moves an objective retracted with the Escape
switch back to the original position.
(6) Escape switch (objective retraction)
Moves the objective to the retracted position
(approximately 1.5 mm below the reference
position).

(3) Epi Shutter switch
(epi illumination shutter
operation)
(1) Coarse/Fine/ExFine
switch/indicator
selector/indicator)
(6) Escape switch
(objective retraction)
(5) Refocus switch
(objective refocusing)
(4) FL Block switch
(filter block selector)
(2) Focus knob
Chapter 3 Operation
3.4 Optical Path Selection
62
3.4 Optical Path Selection
The microscope has several observation ports. Use the optical path selector switches on front operation
panel to distribute the optic image to the ports. The switch for the selected port will be lit, and the port name
will be displayed on the status display panel.

3.4.1 For Ti-E
L80
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1.5X
L80
EYE
R100 L100

Figure 3.4-1 Ti-E optical path selector switches

Switch Light distribution
(1) EYE Eyepiece observation port 100%
(2) L100 Left side port 100%
(3) R100 Right side port 100%
(4) L80
Left side port 80%,
eyepiece observation port 20%
* (3) or (4) may be set to R80 (right side port 80%,
eyepiece observation port 20%) depending on your
purchased configuration.

3.4.2 For Ti-E/B
B100
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1.5X
B100
EYE
R100 L100

Figure 3.4-2 Ti-E/B optical path selector switches

Switch Light distribution
(1) EYE Eyepiece observation port 100%
(2) L100 Left side port 100%
(3) R100 Right side port 100%
(4) B100 Bottom port 100%
* (3) may be set to R80 (right side port 80%, eyepiece
observation port 20%) or L80 (left side port 80%,
eyepiece observation port 20%), depending on your
purchased configuration.

3.4.3 Eyepiece Base Unit Options
S
ID
E
EYE

S
ID
E
EYE

Figure 3.4-3 Eyepiece base unit side ports

TI-T-BS Eyepiece Base Unit with Side Port and
TI-T-BPH External Phase Contrast Eyepiece Base
Unit have a side port equipped with a C-mount
adapter.
When using these eyepiece base units, you will be
able to use both the eyepiece observation port and
the base unit side port. To do so, select the eyepiece
observation port with the optical selector switches.
To switch between the eyepiece observation port
and the side port, use the optical path selector knob
on the right side of the eyepiece base unit.

Optical path
selector knob
TI-T-BS Eyepiece Base
Unit with Side Port
TI-T-BPH External Phase
Contrast Eyepiece Base Unit
Optical path
selector knob
Side port Side port
Chapter 3 Operation
3.5 Filter Operation
63
3.5 Filter Operation

Figure 3.5-1
Filter sliders (Dia Pillar Illuminator 100W)

Filter sliders can be attached to the pillar illuminator
(up to four on the 100W model, and three on the
30W model).
Use the necessary filters by attaching them to the
filter sliders.
The following filters are available:

Table 3.5-1 List of filters
Filter name Description
ND2
Adjusts the brightness for normal microscopy or photomicroscopy (ND: Neutral Density).
Reduces the light intensity to 1/2. (Transmittance: Approx. 50%)
ND4
Reduces the light intensity to 1/4. (Transmittance: Approx. 25%)
ND8
Reduces the light intensity to 1/8. (Transmittance: Approx. 12.5%)
ND16
Adjusts the brightness for normal microscopy or photomicroscopy. (ND: Neutral Density)
Reduces the light intensity to 1/16. (Transmittance: Approx. 6.3%)
NCB11
Corrects the color temperature for normal microscopy or filming by daylight type color TV
cameras (NCB: Neutral Color Balance). Color reproducibility is optimal when this filter is
placed in the optical path and the lamp voltage is matched to the lamp rating. Keep this
filter out of the optical path when filming in black and white.
GIF
Green interference filter. Improves the contrast for when observing under a monochrome
light or when filming in black and white.
D
Diffusion filter. This filter is made of frosted glass and will diffuse light. Use this filter to
equalize the illumination.
HA
Absorbs the heat rays in the illumination (HA: Heat Absorption). This filter reduces the
effect of heat on the specimen. While the dia pillar illuminators have a built-in heat
insulation filter, it is recommended that this filter be used for specimens that are sensitive to
heat.
IR
Infrared filter for PFS (IR: Infra-Red). Use this filter when using the PFS function with dia
illumination.

Filter sliders
Chapter 3 Operation
3.6 Field Diaphragm Operation
64
3.6 Field Diaphragm Operation
(TI-DH Dia Pillar Illuminator 100W only)

Figure 3.6-1 Field diaphragm knob

The field diaphragm is used to limit the irradiation
area of the lamp to the microscope's field of view.
As viewed from the top of the pillar illuminator, a
counterclockwise rotation of the field diaphragm
knob increases the diameter of the irradiation area,
and a clockwise rotation decreases the diameter of
the irradiation area.
Usually, the irradiation area is adjusted to a size that
circumscribes (or inscribes) the field of view. An
unnecessarily large irradiation area will result in stray
light and flare, thereby reducing the contrast of the
optic image.
Field diaphragm adjustment is particularly important
when performing photomicroscopy. Typically,
adjusting the irradiation area to be slightly larger
than the size of the image sensor (indicated by the
capture area frame) will yield favorable results. Avoid
making the size of the irradiation area too close to
the size of the image sensor, as it may result in
vignetting.
The field diaphragm of the TI-DS Dia Pillar
Illuminator 30W has a fixed opening size. It
cannot be adjusted.

Field diaphragm adjustment

Move field diaphragm image into center of field of
view. Adjust its size to match field of view.
Figure 3.6-2 Field diaphragm adjustment

1. Move the 10x objective into the optical path.
2. Rotate the field diaphragm knob on the dia
pillar illuminator until the field diaphragm
image is visible in the field of view.
3. Adjust the focus onto the field diaphragm
image by rotating the condenser focus knob
on the dia pillar illuminator.
of the field of view by rotating the two
condenser centering knobs on the dia pillar
illuminator.
of the field of view by rotating the two
condenser centering knobs on the dia pillar
illuminator.
Condenser
centering knobs
Condenser
focus knob
Condenser
focus knob
Field diaphragm
knob
Field diaphragm image
Field of view
Chapter 3 Operation
3.7 Aperture Diaphragm Operation
65

Figure 3.7-1 Aperture diaphragm open/close lever

70~80
100

Adjust the size of the aperture diaphragm image
to 70-80% the size of the objective pupil plane.
Figure 3.7-2 Aperture diaphragm adjustment

The aperture diaphragm adjusts the numerical
aperture of the illumination system.
By adjusting the aperture diaphragm, you can adjust
the resolution, brightness, contrast, and focal depth
of the microscope image. Closing the aperture
diaphragm will reduce the resolution and brightness,
and increase the contrast and focal depth. These
properties are interrelated, and cannot be adjusted
independently. Adjust the aperture diaphragm
according to the specimen and purpose.
Aperture diaphragm adjustment is particularly
important for bright-field microscopy, DIC
microscopy, and photomicroscopy. Typically,
adjusting the aperture diaphragm to 70-80% of the
numerical aperture of the objective will result in an
appropriate contrast and a favorable image.
Adjust the aperture diaphragm while looking at the
actual diaphragm image. Move the aperture
diaphragm open/close lever to the left to reduce the
aperture size, or to the right to increase the aperture
size. Adjust the aperture diaphragm so that the size
of the diaphragm image is at 70-80% of the size of
the objective pupil plane.

Adjustment with Bertrand lens on eyepiece tube

B

Figure 3.7-3 Adjustment with Bertrand lens

When using TI-TD Eyepiece Tube B or TI-TERG
Ergonomic Eyepiece Tube, use the Bertrand lens in
the eyepiece tube for adjustment.
moving the Bertrand lens in/out lever at the lower
front of the eyepiece tube to position B. Rotate the
Bertrand lens focusing knob on the right to adjust the
focus. This will allow you to view the objective pupil
plane (a bright circle) and the aperture diaphragm
image.

Adjustment with centering telescope

Figure 3.7-4 Adjustment with centering telescope

When using TI-TS Eyepiece Tube B, use the
centering telescope for adjustment.
Remove one of the eyepieces. Using the adapter,
attach a centering telescope instead. Rotate the
eyepiece of the centering telescope to adjust the
focus. This will allow you to view the objective pupil
plane (a bright circle) and the aperture diaphragm
image.

Aperture diaphragm
open/close lever
Objective pupil
plane
Aperture diaphragm
image
Bertrand lens
focusing knob
Set to position B
Flange Eyepiece
Chapter 3 Operation
66
Precautions on condenser and aperture diaphragm
Be sure to fully open the aperture diaphragm when performing phase contrast microscopy with the TI-C
Condenser Turret. If the aperture diaphragm is not fully open, the optical path will be blocked.
The aperture diaphragm of the ELWD-S condenser is used only for bright-field microscopy. This aperture
diaphragm is independent of the phase contrast annular diaphragm. Adjustment of the aperture diaphragm
will not affect the annular diaphragm.

Chapter 3 Operation
3.8 Condenser Operation
67
3.8.1 TI-C Condenser Turret (System Condenser)

(system condenser)

The condenser has two functions: the first is to focus
the dia illumination light, and the second is to apply
optical modulation to enable the various microscopy
methods.
Traditionally, the condenser had to be changed
depending on the microscopy method.
On the other hand, a system condenser can have up
to five condenser cassettes in its turret, each with
different optical elements. Thus, the microscopy
method can be changed simply by rotating the turret.
The condenser cassettes can be freely arranged on
the turret, as long as they are compatible with the
condenser lens (there are six types). Condenser
cassettes can be changed without removing the
condenser from the microscope, allowing several
microscopy methods to be used within a short time
span.
When using the TI-CT-E Motorized Condenser
Turret, refer to the instruction manual provided
with TI-HUBC/A Hub Controller A.

Phase contrast microscopy
When performing phase contrast microscopy, use a condenser cassette that has the same Ph code as the
objective and the external phase contrast ring, and center the annular diaphragm after moving the condenser
cassette into the optical path. Be sure to fully open the aperture diaphragm. If the aperture diaphragm is not
fully open, the optical path will be blocked.

Comparison of condenser lenses
Table 3.8-1 Comparison of condenser lenses
LWD condenser lens CLWD condenser lens ELWD condenser lens
NA 0.52 0.72 0.3
Working distance 30 mm 13 mm 75 mm
Supported
microscopy methods
Bright-field, phase
contrast, DIC
Bright-field, phase contrast Bright-field, phase contrast
Supported
condenser cassettes
Bright-field: A
Phase contrast:
PhL, Ph1, Ph2, Ph3
DIC: DIC L, DIC M, DIC H
HMC: MC1, MC2, MC3
Bright-field: A
Phase contrast:
Ph1, Ph2, Ph3, Ph4
Bright-field: A
Phase contrast:
PhL, Ph1, Ph2
Remarks
Supporting condenser
refocus clamp
Requires TI-DF condenser
adapter.

* Condenser lenses of HMC, oil immersion type for dark-field, and dry type for
dark-field are also available.
Refocusing clamp
Condenser
centering knobs
Condenser
focus knob
Condenser
focus knob
Condenser lens
Aperture diaphragm
open/close lever
Turret and
condenser cassette
Chapter 3 Operation
68
3.8.2 ELWD-S Condenser

Figure 3.8-2 ELWD-S Condenser

The ELWD-S Condenser supports bright-field
microscopy and phase contrast microscopy. The
ELWD-S Condenser can be used with both 100W
and 30W pillar illuminators.

3.8.3 High NA Condenser Holder

Figure 3.8-3 High NA condenser holder

The high NA condenser holder is used with the high
NA condenser lens for differential interference
contrast (DIC) microscopy, phase contrast
microscopy, and bright-field microscopy under high
magnification.
The holder can be attached only to the Dia Pillar
Illuminator 100W. And the high NA common
condenser lens is required, too.
The dust-proof plate can be attached also to the
back side by detaching the dust-plate clamp screws
from the original positions and reattaching them to
the opposite side clamp screw holes.
Condenser lens
Turret
Condenser
centering knob
(both sides)
Centering knob
clamp screw
(both sides)
Slider lever
Extension tube
(provided with the
high NA common
condenser lens)
Aperture
diaphragm knob
Condenser cassette
clamp screws
Condenser cassette
clamp screws
Dust-proof plate
clamp screws
Dust-proof plate
Front side
Back side
(dry type or oil immersion
type)
Slider clamp
screw
Cassette
slider
Chapter 3 Operation
3.9 Eyepiece Tube Operation
69
3.9.1 Diopter Adjustment
Diopter adjustment corrects the difference in the left and right fields of view, making binocular observation
easier. The eyepiece tube length will be maintained, allowing for the objective to perform optimally with
minimal focus loss upon objective change.
To perform diopter adjustment, follow the instructions below.

B

Figure 3.9-1 Diopter adjustment

1. Under bright-field microscopy, adjust the
focus of the 10x objective onto the specimen.
2. On each eyepiece, rotate the diopter
adjustment ring to align its lower end with the
marking on the eyepiece.
This will be the reference position for diopter
adjustment.
rotating the diopter adjustment ring on the
left eyepiece.
Do not touch the focus knobs at this time.
7. Repeat steps 3 through 6 two times.
8. Adjust the right eyepiece.
Repeat steps 2 through 7, but this time using the
right eyepiece instead of the left.

3.9.2 Interpupillary Distance Adjustment

Figure 3.9-2 Interpupillary distance adjustment

Interpupillary distance adjustment adjusts the
distance between the eyepieces to better suit the
user. This will make binocular observation easier.
When diopter adjustment is complete, move the 10x
objective into the optical path, and adjust the focus
onto the specimen. Look into the eyepieces with
both eyes, and adjust the interpupillary distance of
the binocular part so that the two fields of view
overlap into one.
The binocular part has an interpupillary distance
scale. It is recommended that you remember your
own interpupillary distance for easier adjustment in
the future.

Diopter
adjustment ring
Marking
Adjust interpupillary
distance of binocular part
to merge fields of view.
Chapter 3 Operation
70
3.9.3 Eyepiece Tube Shutter Operation
B

Figure 3.9-3 Shutter open/close lever

TI-TD Eyepiece Tube B and TI-TERG Ergonomic
Eyepiece Tube have a built-in manual shutter
mechanism.
Pull out the shutter operation lever on the right side
of the eyepiece tube to push the shutter into the
optical path. Push in the lever to the limit to open the
shutter.
* TI-TS Eyepiece Tube B is not equipped with a
built-in shutter.

3.9.4 Bertrand Lens Operation
B

Figure 3.9-4 Bertrand lens operation

TI-TD Eyepiece Tube B and TI-TERG Ergonomic
Eyepiece Tube have a built-in Bertrand lens.
moving the Bertrand lens operation lever at the lower
front to position B. Rotate the Bertrand lens
focusing knob on the right to adjust the focus. This
will allow you to view the objective pupil plane (a
bright circle) and the aperture diaphragm image.

Position Optical element Description
O Blank Use to observe the microscope image with the eyepiece tube.
B Bertrand lens
Use to observe the objective pupil plane. Use the focusing knob to
adjust the focus onto the pupil plane. The Bertrand lens is used for
aperture diaphragm adjustment, as well as for centering during phase
contrast microscopy. It can also be used to observe the tip of the
manipulator above the objective, if using a manipulator.

* TI-TS Eyepiece Tube B is not equipped with a
Bertrand lens.

Bertrand lens
focusing knob
Set to position B
Shutter open/close lever
Push: shutter open
Pull: shutter close
Chapter 3 Operation
3.10 Focusing Mechanism Operation
71
3.10.1 Focus Knob Operation
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
B

Figure 3.10-1 Focus knobs and nosepiece movement

Ti-E has a motorized focusing mechanism. The
nosepiece can be moved up and down by rotating
the focus knobs on the sides of the microscope
body. The figure on the left illustrates the relationship
between the rotational direction of the focus knobs
and the vertical motion of the objective.
* The stroke for focus adjustment is approximately
9.5 mm.
Focus knob resolution selector
The resolution for focus knob rotation can be set to
one of the following three levels:
Setting Distance traveled per rotation
Coarse
300 m per rotation
(reference value) *
Fine 50 m per rotation
ExFine (extra fine) 6.25 m per rotation
* When set to COARSE mode, the distance
traveled by rotating a focus knob will vary
depending on how fast the knob is rotated.
Focus knob resolution, Z-axis position display
The current focus knob resolution and Z-axis
position will be displayed on the status display panel
on the front.
Display example for Z-axis position and
focus knob resolution
Z:_ 124.225um
E100_Fine

Coarse/Fine/ExFine
switch/indicator
selector/indicator)
Focus knob
Chapter 3 Operation
72
3.10.2 Z-RESET Switch Operation
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Z:__128.735um
E100_Coarse

B100
EYE
DISPLAY
MEMORY
PFS
ON RECALL
Z-RESET BRIGHTNESS
R100 L100
FOCUS
1X
1.5X

Z:____0.000um
E100_Coarse

Figure 3.10-2 Z-RESET switch operation

The Z-axis position of the focus mechanism,
displayed on the status display panel, increases
when the nosepiece is elevated, and decreases
when the nosepiece is lowered.
Once you have set the focus at the desired height,
pressing the Z-RESET switch on the front operation
panel and resetting the Z-axis position display will
allow you to use the set position as a reference point
for focus adjustment.

Adjust focus.
Z-axis position
is displayed.
Press Z-RESET switch.
Z-axis position
display is reset.
Chapter 3 Operation
73
3.10.3 Retraction and Refocusing of Objective
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 3.10-3 Escape switch, Refocus switch

(1) Adjust the focus.

(2) Press the Escape switch.

(3) Press the Refocus switch.

Figure 3.10-4
Retraction and refocusing of objective

The Escape switch (objective retraction) and
Refocus switch (objective refocusing) on the right
side of the microscope can be used to move the
objective to and back from the retracted position
(approximately 1.5 mm below the reference
position).
With the focus on the specimen, press the Escape
switch to record the current position and lower the
objective to the retracted position. The objective can
be brought back up to the focused position by
pressing the Refocus switch, making refocusing
easier. This function is useful for temporarily moving
the objective out of the way, for example when
replacing the specimen or changing the objective.
When the objective is at the retracted position, its
height cannot be adjusted by rotating the focus
knobs.
When the objective at the retracted position and
the Escape and the Refocus switches are pressed
together, the focus position memory is cleared
and the objective can be moved with the focus
knob.
When using the TI-ND6-PFS Perfect Focus Unit
or the TI-ND6-E Motorized Sextuple DIC
Nosepiece in combination with the TI-RCP
Remote Control Pad, the system can be set to
retract and refocus the objective automatically
when the objective is switched. For details, refer
to the instruction manual provided with the
TI-RCP Remote Control Pad.

Escape switch
Refocus switch
Objective
lowered to
retracted
position.
Objective
returned to
original
position.
Chapter 3 Operation
3.11 Objective Operation
74
3.11.1 Phase Contrast Objectives

Figure 3.11-1 Phase contrast objective (example)

Phase contrast objectives are labeled with a Ph
code (PhL, Ph1, Ph2, or Ph3). When performing
phase contrast microscopy, use an annular
diaphragm or condenser cassette that has the same
Ph code as the objective, regardless of the
magnification of the objective.

3.11.2 Cover Glass Thickness

Figure 3.11-2 Cover glass thickness (example)

Objectives are labeled with the supported cover
glass thickness. For example, /0.17 indicates a
cover glass thickness of 0.17 mm.
When observing at high magnification through
glass that is thicker than the supported glass
thickness (i.e. when observing a specimen in a
Petri dish), it is recommended that you use an
objective with a correction ring so that the optical
system can be adjusted accordingly. (See Section
3.11.3, Objectives with Correction Ring)

Glass thickness of 0.17 mm

Figure 3.11-3 Glass thickness of 0.17 mm

When using an objective labeled 0.17, face the
cover glass downward, and set the specimen so that
the cover glass faces the objective. (The cover glass
has a thickness of 0.17 mm.)

Glass thickness of 1.2 mm

Figure 3.11-4 Glass thickness of 1.2 mm

When using an objective labeled 1.2, face the
cover glass upward, and set the specimen so that
the glass slide faces the objective. (The glass slide
has a thickness of 1.2 mm.)

Ph code
(example: Ph2)
Cover glass
Glass slide
Glass slide
Cover glass
Cover glass thickness
(example: /0.13-0.20)
Chapter 3 Operation
75
3.11.3 Objectives with Correction Ring

Figure 3.11-5 Objective with correction ring
(example)

Inverted microscopes are sometimes used to
observe though the bottom plate (glass or plastic) of
a Petri dish or a culture vessel. In such a case, the
microscope may not perform optimally with standard
objectives (for glass covers with a thickness of 0.17
mm), as the thickness of the bottom plate varies
from container to container.
By using an objective with a correction ring, you will
be able to compensate for the thickness of the
bottom plate.
Note, however, that correction is not possible where
there is a change in the thickness of the bottom plate
(i.e. around the periphery of the container). Use the
correction function where the thickness of the bottom
plate is uniform.

Correction ring adjustment
1. Adjust the objective correction ring to match
the reading on the scale to the thickness of
the containers bottom plate.
For the thickness of the bottom plate, take an
actual measurement or refer to the specifications
provided by the manufacturer.
An acrylic concentric ring is useful as it will allow
you to view the operating parts from above the
stage as you work.
2. Focus on the specimen by rotating the focus
knobs.
3. If the resolution and contrast of the image are
poor, slightly rotate the correction ring on the
objective in either direction.
This will shift the focus slightly. Readjust the
focus by turning the focus knob.

4. If resolution and contrast are improved,
rotate the correction ring slightly in the same
direction, and readjust the focus.
If resolution and contrast are lost, rotate the
correction ring in the opposite direction by
approximately twice the amount rotated in the
previous step. Readjust the focus.
Repeat the process to obtain the optimal image.
It is recommended to note the optimal
correction ring setting for use as reference in
using containers of different thicknesses.
The 0 mm position of the correction ring
corresponds to the position for observing a
no-cover-glass specimen on an upright
microscope.

Scale
Correction ring
Chapter 3 Operation
76
3.11.4 Oil Immersion Objectives

Figure 3.11-6 Oil immersion objective (example)

Objectives with the Oil label are oil immersion
objectives.
When using an oil immersion objective, fill the space
between the objective tip and the specimen with oil
(Nikon Immersion Oil). When performing epi-fl
microscopy with an epi-fl oil immersion objective,
use non-fluorescent oil.

Applying Oil

Figure 3.11-7 Applying oil immersion

1. Lower the objective by rotating the focus
knobs.
2. Taking care not to let bubbles form, apply the
bare minimum amount of oil onto the tip of
the objective.
If too much oil is applied, the excess oil may
overflow onto the stage and other parts. Use as
little oil as possible (just enough to fill the space
between the objective tip and the specimen), and
take care not to allow the oil to get on other
parts.
3. Place the specimen onto the stage.
4. Slowly raise the objective by rotating the
focus knobs, allowing the oil to fill the space
between the objective tip and the specimen.
5. Check that no air bubbles have formed in the
oil.
Bubbles in the oil will adversely affect the
viewing of the image. Refer to the following
section and check for bubbles.
Oil
marking
Chapter 3 Operation
77
Checking for air bubbles
(1) Objective pupil plane under observation
with Bertrand lens

(2) Field of view with focus of Bertrand lens
shifted from the above state

Figure 3.11-8
Air bubble observation with Bertrand lens (example)

To check for air bubbles, observe the objective pupil
plane. The objective pupil plane can be observed by
rotating the Bertrand lens operation lever to position
B and adjusting the focus with the Bertrand lens
focusing knob, or by replacing an objective with a
centering telescope and adjusting the focus with its
eyepiece.
If you detect bubbles in the oil, attempt to remove
them by rotating the nosepiece slightly to move the
oil-immersed objective back and forth in the oil one
or two times. If the bubbles cannot be removed, wipe
off the oil, and reapply new oil.

Removing oil
After using an oil immersion objective, wipe the oil
off from its tip.
To remove the oil, gently wipe two or three times with
a lens tissue or gauze dampened with petroleum
benzine. It is recommended that you avoid using the
same area of the tissue or gauze repeatedly. After
wiping with petroleum benzine, wipe with absolute
alcohol (ethyl or methyl) for a better finish.

If petroleum benzine is unavailable, use methyl
alcohol. However, as methyl alcohol does not clean
as well as petroleum benzine, it will be necessary to
wipe a few more times. (Three to four wipes are
usually sufficient.)
When wiping oil off of the specimen, take care not
to damage the specimen.

Caution

Residual oil on an oil-immersion objective or oil adhered to the tip of a dry-type objective
will degrade the image quality significantly. After use, thoroughly wipe off all oil, and make
sure that no oil adheres to the tips of other objectives.
Absolute alcohol and petroleum benzene are highly flammable. Handle with care. Do not
use near an open flame, or operate a power switch in the vicinity.

Reapplying oil
When performing oil immersion repeatedly, use the
Escape and Refocus switches for faster focus
adjustment.

A 25 mm or acrylic concentric ring is useful as it will
allow you to apply the oil via its oiling notch, without
needing to remove the specimen (i.e. Petri dish). Set
the concentric ring so that its notch matches the
rotational direction of the nosepiece, hold the
nosepiece so that the objective is aligned with the
notch, and apply the oil.

Light partially blocked
in upper section of
field of view.
Air bubble recognized
in upper section of
field of view.
Chapter 3 Operation
78
3.11.5 Water Immersion Objectives

Figure 3.11-9 Water immersion objective (example)

Objectives with the WI label are water immersion
objectives. (Those with long operating distances are
for upright microscopes.)
When using a water immersion objective, fill the
space between the objective tip and the specimen
with deionized or distilled water.

Caution

Do NOT use tap water. If tap water is used, impurities may adhere to and solidify on the
lens, causing the lens to become scratched when being cleaned.
Plan Apo 60xWI (NA=1.2) is equipped with a correction ring to achieve optimal aberration
on cover glasses of different thicknesses. A reading of 17 on the scale indicates 0.17 mm.
When using a cover glass, measure the thickness of the cover glass with a micrometer, and
adjust the correction ring for the thickness for a more accurate correction.

WI marking
Chapter 3 Operation
3.12 Pillar Illuminator 100W Operation
79
This section describes operations specific to TI-DH Dia Pillar Illuminator 100W.

3.12.1 Condenser Refocusing Clamp

Figure 3.12-1 Pillar Illuminator 100W

Form the field diaphragm image on the specimen
surface by rotating the condenser focus knob. Rotate
the condenser refocusing clamp clockwise to the
limit to mark this position.
When the condenser is elevated to change the
specimen, it can easily be brought back down to the
initial position (at which the field diaphragm image is
formed) by rotating the condenser focus knob to the
limit. This function is useful for use with high
magnification condensers or when the pillar cannot
be inclined. The condenser refocusing clamp has a
range of motion of 13 mm.

3.12.2 Condenser Mount Rotation

Figure 3.12-2 Condenser mount

The condenser mount can be rotated if the
condenser mount rotation clamp screw is loosened.
Use this function to adjust the orientation of the turret
when using the DIC attachment.
When using the system condenser without a
polarizer on the condenser holder (i.e. for bright-field
or phase contrast microscopy), this function can also
be used to rotate and fix the turret. This will allow
you to secure a space for manipulator attachment.

Condenser
refocusing clamp
Condenser
focus knob
Condenser
focus knob
Condenser mount
rotation clamp screw
Condenser mount
Chapter 3 Operation
80
3.12.3 Pillar Inclination

Figure 3.12-3 Pillar inclination

When replacing a large specimen, the pillar can be
inclined to secure working space.
To incline the pillar, loosen and release the clamp
screw on its back. Hold the front side of the dia
illuminator, and slowly let the pillar incline backward.
Under normal use, the clamp screw on the pillar
can be left released. However, be sure to tighten
the clamp screw when attaching relatively heavy
objects to the pillar, so as to prevent it from
falling.

Caution

When moving the pillar into and out of an incline, take caution not to get your hands and
fingers caught in the hinge.
When attaching relatively heavy objects to the pillar, be sure to secure them properly. A
loose screw may result in the attachment falling off when the pillar is inclined. In particular,
be sure to properly secure high-intensity lamphouses and lamphouse adapters.

3.12.4 Device Attachment Screw Holes

Figure 3.12-4 Device attachment screw holes

Four M4 screw-taps are provided on the front of the
pillar for attaching devices such as manipulators.
Use the upper two holes to attach devices that
should be moved out of the way when the pillar is
inclined. Use the lower two holes to attach devices
that should remain positioned over the stage when
the pillar is inclined.

Clamp screw
M4 screw-taps
(x4)
Chapter 3 Operation
3.13 Rectangular Mechanical Stage Operation
81
3.13 Rectangular Mechanical Stage Operation

Figure 3.13-1 Rectangular Mechanical Stage

The knob on the rectangular mechanical stage uses
a universal joint, and can be operated freely at any
angle.
The stage is provided with screw-taps on both the
top and bottom surfaces for attaching devices such
as manipulators.
The rectangular mechanical stage is typically
attached with the knob positioned in the far right.
It is also possible to rotate the stage 180 degrees
and attach it with the knob in the near left.

25 mm bore
(with oiling notch)
40 mm bore
Figure 3.13-2 Concentric rings

Two concentric rings of different sizes are provided
with the microscope (25 mm and 40 mm). Use
whichever is appropriate depending on the size of
the specimen.
When using the 40 mm ring, the objective may
collide with the bottom surface of the stage if the
nosepiece is rotated with the stage moved out of the
observation area. In such a case, first lower the
nosepiece to the limit, and then switch the objective.

Caution

The stage rack will protrude when the stage is operated. When operating the focus knobs or
the nosepiece, take caution not to strike your hands against the rack. Contact with the edges
of the rack may result in injury.

Figure 3.13-3 Rectangular Mechanical Stage Rack

Knob
Rack remains protruded.
Chapter 3 Operation
3.14 PFS (Perfect Focus System) Operation
82
This section describes the operation of the PFS (Perfect Focus System).
To use PFS, attach the PFS Motorized Nosepiece and the PFS offset controller to Ti-E or Ti-E/B, and use
PFS ready objectives. You will also need to register the objective information on the microscope. For
assembly details, refer to Chapter 4, Assembly.

3.14.1 PFS Overview
The PFS is used to keep focus for long-term observations. It automatically corrects focus drift caused by
thermal expansion of the stage or temperature change of the specimen.
The PFS uses near-infrared rays (IR wavelength, 870 nm) that do not interfere with normal observations to
detect the boundary surface
(NOTE 1)
between the cover glass
(NOTE 2)
and the aqueous solution of the
specimen, and then maintains focus by automatically tracking the vertical shift of that surface.
It can be useful, for example when continuously observing or photographing changes in living cells, as it is
not affected by changes in the specimen or by discoloration under fluorescent light, and is capable of
maintaining the focus by compensating for minor focus shifts that may occur with time or by stage
movements.
NOTE 1 : When using a dry objective, the boundary surface between the cover glass and air will be detected.
NOTE 2 : The cover glass refers to the glass on the bottom surface of a glass-bottomed dish.

Features of PFS
The PFS uses infrared rays to keep focus. As infrared rays have minimal effect on the specimen, and do
not interfere with fluorescent observation, the focus can be maintained while performing fluorescent
observation.
The optical offset function allows for manual adjustment of the focus position.
The vertical position (Z-axis position) of the nosepiece and the focus offset can be registered
independently for each objective.
Focus can be maintained for any target within the field of view.
Focus can be maintained with any observation camera or method (including macroscopic), unlike with the
image contrast method that can only be used with certain cameras.
Chapter 3 Operation
83
Supported specimens
The PFS function can be used with any specimen that satisfies the following conditions.
Cells attached to the cover glass of a glass-bottomed dish.
Cover glass Thickness: 150 to 180 m (No.1S)
Refractive index: 1.5
Culture solution Depth: 3 mm or greater
Refractive index: Approximately 1.33

Figure 3.14-1 PFS overview

Unsupported specimens
In-focus observation with the PFS may be difficult on the following specimens, for reasons such as weak IR
reflectivity or excessive scattered light.
1. Fixed specimens
In general, fixed specimens are filled with an encapsulating medium. Due to the high refractive index of
this medium, the difference in the refractive index between the cover glass and the specimen is
decreased. Thus, the amount of reflected light may be insufficient for surface detection.
2. Sliced specimens
Sliced specimens are thick, and scatter a large amount of light. This makes it difficult to capture the
relatively small amount of reflective light from the boundary surface.
3. Specimens with large amounts of scattered or reflected light
For the same reason as for the sliced specimens, non-sliced specimens that scatter a large amount of
light are also unsupported.
4. Cells attached to a container with a glass bottom thicker than 170 m
When using a dry objective with a specimen in thick glass, the boundary surface may not be detectable
due to insufficient offset. (The boundary surface may be detectable if using an oil immersion objective.
However, use of No.1S cover glass is recommended.)
5. Plastic dishes
The use of plastic dishes is not recommended, as their boundary surfaces are not as precise as those of
glass.
6. Dirty cover glasses (oil stains, dust)
The PFS maintains focus by detecting the boundary surface of the cover glass. Contaminations on the
cover glass will degrade the precision of the detection. Be sure to clean the cover glass before use.
Boundary surface
(Water/oil immersion)
Boundary surface
(Dry)
Refractive index
Oil: 1.514
Air: 1.0
Water: 1.33
Cover glass: approx. 1.5
Culture solution: approx. 1.35
t = 165 15 m
(Standard: No.1S)
t
3 mm
Chapter 3 Operation
84
Supported objectives
The following objectives can be used for in-focus observation with the PFS. Use of other objectives will result
in an error, and PFS: ER1 will be displayed on the status display panel.
Table 3.14-1 PFS ready objectives
Model name NA
WD
(mm)
Type Model name NA
WD
(mm)
Type
Apo TIRF 60xH 1.49 0.12 Oil Plan Fluor 10x 0.3 16 Dry
Apo TIRF 100xH 1.49 0.12 Oil Plan Fluor DLL 10x 0.3 16 Dry
Plan Apo 4x 0.2 15.7 Dry Plan Fluor ELWD 20xC 0.45 7.4 Dry
Plan Apo 10x 0.45 4 Dry Plan Fluor ELWD ADL 20xC 0.45 7.4 Dry
Plan Apo 20x 0.75 1 Dry Plan Fluor ELWD DM 20xC 0.45 7.4 Dry
Plan Apo 40x 0.95 0.14 Dry Plan Fluor 40x 0.75 0.72 Dry
Plan Apo DM 40x 0.95 0.14 Dry Plan Fluor 40xH 1.3 0.2 Oil
Plan Apo 60xHA 1.4 0.21 Oil Plan Fluor DLL 40x 0.75 0.72 Dry
Plan Apo 60xWI 1.2 0.22 WI Plan Fluor ELWD 40xC 0.6 3.7-2.7 Dry
Plan Apo DM 60xHA 1.4 0.21 Oil Plan Fluor ELWD ADL 40xC 0.6 3.7-2.7 Dry
Plan Apo DM 60xH 1.4 0.21 Oil Plan Fluor ELWD DM 40xC 0.6 3.7-2.7 Dry
Plan Apo 100xH 1.4 0.13 Oil Plan Fluor 100x H 1.3 0.2 Oil
Plan Apo DM 100xH 1.4 0.13 Oil Plan Fluor ADH 100x H 1.3 0.19 Oil
Plan Apo TIRF 60xH 1.45 0.13 Oil Plan Fluor DLL 100x H 1.3 0.2 Oil
Plan Apo TIRF 100xH 1.45 0.13 Oil S Fluor 4x 0.2 15.5 Dry
Plan Apo VC 60xWI 1.2 0.27 WI S Fluor 10x 0.5 1.2 Dry
Plan Apo VC 60xH 1.4 0.13 Oil S Fluor 20x 0.75 1.00 Dry
Plan Apo VC 100xH 1.4 0.13 Oil S Fluor DL 20x 0.75 1.00 Dry
S Fluor 40xH 1.3 0.22 Oil
S Fluor DL 40xC 0.9 0.3 Dry
S Plan Fluor ELWD 20xC 0.45 8.2-6.9 Dry
S Plan Fluor ELWD ADM 20xC 0.45 8.2-6.9 Dry

To perform the PFS function, you will need to register the objective information on the microscope.
For registration details, refer to Chapter 4, Assembly.

Chapter 3 Operation
85
3.14.2 Starting and Stopping In-focus Observation with the PFS
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 3.14-2 Preparation for in-focus observation

(1) Start the PFS function
MEMORY
PFS
ON RECALL
FOCUS
MEMORY
PFS
ON RECALL
FOCUS

(2) Place objective into focusable range
MEMORY
PFS
ON RECALL
FOCUS

(3) Focus on the reference position (boundary
surface)
MEMORY
PFS
ON RECALL
FOCUS

Figure 3.14-3 Starting the PFS function

1. Select an objective, and place a specimen
onto the stage.
Place a PFS ready objective into the optical
path. (See PFS ready objectives on page 84.)
Perform water/oil-immersion procedures as
necessary.
2. Place an IR filter into the optical path.
Attach an IR filter (45 mm) to the filter slider on
the pillar illuminator, and then place the IR filter
into the optical path.
For epi illumination, attach an IR filter (25 mm)
to the filter sliders on the epi illuminator.
3. Place the dichroic mirror into the optical path
by moving the DICHROIC MIRROR - IN/OUT
lever on the top of the PFS Motorized
Nosepiece to the IN position.
4. Focus on the specimen by rotating the focus
knobs on the microscope.
5. Start the PFS function by pressing the
PFS-ON switch on the front operation panel.
The PFS-ON switch indicator lights up, and the
PFS function will start. The focus knobs will be
disabled while the PFS function is running.
PFS function is running
PF100x_Z:__124.375um
E100_Coarse_PFS:On_
When the objective enters the focusable range,
the FOCUS indicator on the front operation panel
will begin to blink. When the focus is on the
reference position (boundary surface), the
indicator will light up.
When the PFS function is enabled, the focus
mechanism will be controlled automatically to
maintain the focus on the reference position.
* The focusable range is defined for each
objective, and is the region within which the
PFS function is enabled for that objective.

4
3
1
2
ON switch lit
FOCUS
blinking
FOCUS lit
Chapter 3 Operation
86

P
F
S
D
O
W
N
U
P
P
F
S
O
F
F
S
E
T
C
O
N
T
R
O
L
L
E
R
F
IN
E
C
O
A
R
S
E


6. Focus on the target of the specimen, by
rotating the offset dial on the PFS offset
controller.
In step 5, the focus is set on the reference
position, not on the actual target.
To set the focus on the actual target, adjust the
offset by rotating the offset dial on the PFS offset
controller. For details, refer to Section 3.14.3,
Offset Adjustment.

MEMORY
PFS
ON RECALL
FOCUS
MEMORY
PFS
ON RECALL
FOCUS

Figure 3.14-5 Stopping the PFS function

7. To stop the PFS function, press the
illuminated PFS-ON switch.
The PFS-ON switch indicator turns off, and the
PFS function stops.
Be sure to stop the PFS function before
replacing the specimen or changing the
objective.
8. When observing without using the PFS
function, remove the dichroic mirror out of
the optical path by rotating the DICHROIC
MIRROR - IN/OUT lever on the top of the PFS
Motorized Nosepiece to the OUT position.

Offset dial
FINE/COARSE
selector switch
ON switch not
lit
ON switch lit
Chapter 3 Operation
87
3.14.3 Offset Adjustment
When the PFS function starts, the Z-axis position of the objective will track the vertical shift of the boundary
surface. To shift the focus onto the desired position, rotate the offset dial on the PFS offset controller.
The offset dial moves the mirror in the PFS Motorized Nosepiece, allowing you to adjust the focus within the
field of view, while maintaining the focused state.
The distance (i.e. the amount by which the focus was moved) from the reference position (boundary surface)
to the position moved to with the offset dial is referred to as offset. This offset will be maintained as the PFS
function tracks the vertical shift of the boundary surface.
* The maximum offset varies depending on the objective. In particular, water/oil immersion objectives are
limited in their downward offset.

P
F
S
D
O
W
N
U
P
P
F
S
O
F
F
S
E
T
C
O
N
T
R
O
L
L
E
R
F
IN
E
C
O
A
R
S
E


PFS Offset Controller operation
The PFS offset controller has an offset dial
component and a base plate that are magnetically
attached.
By changing the orientation of the dial component on
the base plate, the dial can be faced to the left or the
right. The dial can also be faced upward, if used
alone without the base plate.

D O W N
U
P
PFS
OFFSET CONTROLLER
FINE
COARSE

Figure 3.14-7 Offset adjustment

Offset adjustment
FINE/COARSE selector switch
The distance traveled by rotating the offset dial can
be switched between FINE and COARSE. The
selected state can be checked by whether the switch
is pressed in.
Offset dial:
Adjusts the offset. Rotate clockwise to increase the
offset (move the objective closer to the specimen), or
counterclockwise to decrease the offset (move the
objective further form the specimen).

The offset dial is enabled only if the PFS
function is ON (PFS-ON switch is lit).
When using the 4x or the 10x objective, the
offset can be adjusted with fine movements
only, regardless of the FINE/COARSE switch
setting.

Offset dial unit
Base plate
Offset dial
FINE/COARSE
selector switch
Chapter 3 Operation
88
3.14.4 Registration and Restoration of Offset
The offset set during observation can be registered on the microscope. By registering the offset, the offset
can be easily restored after manual adjustments.

Registering the offset

MEMORY
PFS
ON RECALL
FOCUS
MEMORY
PFS
ON RECALL
FOCUS

Figure 3.14-8 Registering the offset

1. Start the PFS function. Focus on the target by
rotating the offset dial on the PFS offset
controller.
2. Press the MEMORY switch.
The MEMORY switch indicator is lit, and the
offset is registered.
The registered offset will be overwritten each
time the MEMORY switch is pressed.
The offset can be registered independently
for each objective on the nosepiece (max. 6).
The registered offsets will be retained until
the system is turned off.
Restoring the offset

MEMORY
PFS
ON RECALL
FOCUS

Figure 3.14-9 Restoring the offset

1. Check that the MEMORY switch is lit.
2 Press the RECALL switch.
The offset lens automatically moves to the
registered offset position.
Manual operation with the focus knobs will be
disabled during the restoration of the offset.
If an offset is not registered (MEMORY switch is
not lit), the RECALL switch will restore the
objective to the zero offset position (initial
position).
Note that the focus position may not match the
boundary surface exactly, depending on the
thickness and material of the cover glass, as well
as on other environment variables.

Press
MEMORY
switch.
MEMORY
switch lit
Press
RECALL
switch.
Chapter 3 Operation
89
3.14.5 If the Objective is Changed
If the objective is changed while the PFS function is running, the PFS function will stop, and the objective will
move as described below.
If an offset is registered:
The objective moves to the registered offset position.
If an offset is not registered:
The objective moves to the zero offset position (initial position). Note that the focus position may not
match the boundary surface exactly, depending on the thickness and material of the cover glass, as well
as on other environment variables.
* The objective will also move in the same manner if the objective is changed while the PFS function is
running.

3.14.6 If the PFS Function Does Not Start
If PFS: DIS is displayed on the status display
panel
There is no reference position (boundary surface)
within the focusable range. Move the objective up
and down by rotating the focus knobs. The PFS
function will start once the boundary surface enters
the focusable region.

If PFS: Out is displayed on the status display
panel
The dichroic mirror is not in the optical path. Place
the dichroic mirror into the optical path by moving the
dichroic mirror operation lever on the top of the PFS
nosepiece to the IN position.
Even if the focus is set on the boundary surface,
the focus may not be set on the observation
target. Adjust the offset as necessary, as
described in Section 3.14.3, Offset adjustment.
In particular, when using a dry objective,
difference in cover glass thickness may cause the
initial position to differ from the boundary surface.
In this case, focus on the observation target with
the PFS offset controller, after the FOCUS
indicator and the PFS-ON switch indicator light
up.

Timeout function (dry objectives only)
If the boundary surface cannot be detected within 5
seconds after pressing the PFS-ON switch to start
the PFS function, the vertical movement of the
objective will stop to prevent collision with the
specimen. This is the timeout function. For
reference, the objective will move approximately 400
m during the 5 seconds.
When the timeout function is activated, the system
will beep, the PFS-ON switch indicator will turn off,
and the vertical movement of the objective will stop.

If PFS: ER2 is displayed on the status display
panel
The timeout function is activated. As there is no
reference position (boundary surface) within the
detectable range, manually focus on the specimen,
and then press the PFS-ON switch again.

90
Assembly
4

Warning

Before assembling or connecting devices, thoroughly read the Safety Precautions at the
beginning of this manual, and heed all warnings and cautions written therein.
To prevent electric shock, fire, and product damage, turn off the power switch on all
devices, and unplug the power cords.

Caution

Take care to avoid pinching your fingers and hands.
Scratches and dirt on optical components (i.e. lenses and filters) will degrade the
microscope image. Keep them free of scratches, dust, fingerprints, and other dirt.
The product is a precision optical instrument. Handle the product with care, and avoid
subjecting it to strong physical shocks. In particular, the accuracy of objectives may be lost
by even weak physical shocks.

Caution

Notes on setup
To use the PFS Motorized Nosepiece, you will need to register the objective information on
the microscope. After assembling the product, connect a PC and set up the microscope
before use.
For setup, use the Ti Control setup software for Ti series. For details on using the
software and setting up the microscope, refer to the Ti Control instruction manual.

This chapter describes the installation, assembly, and setup of the product in the actual sequence.
Assemble the product as described in this chapter.

When using Ti-E or Ti-E/B with TI-HUBC/A Hub Controller A

Refer to the instruction manual provided with TI-HUBC/A Hub Controller A

Chapter 4 Assembly

91
Required tools
2 mm hex screwdriver (x2) (included with product)
3 mm hex wrench (x1) (included with product)
4 mm hex screwdriver (x1) (included with product)

Installation conditions
Refer to the Notes on Handling the Product at the beginning of this manual and install the product in an
appropriate location.

Checking the power supply voltage
When using TE-PS30W Power Supply A or TE-PSE30 Power Supply A with the product, check that the input
voltage indicated on the power supply device matches the power voltage in your area. If the voltages do not
match, do not use the power supply, and contact Nikon. Use of a power supply with the incorrect voltage
rating may result in malfunctions, electric shock, or fire.

Assembly diagram
Stage
Eyepiece
tube
Eyepiece
base unit
Power supply device
To a wall outlet
To a wall outlet
Nosepiece
Objective
Eyepieces
Main body
(microscope base)
TI-DH Dia Pillar Illuminator 100W and
system condenser configuration
TI-DS Dia Pillar Illuminator 30W and
ELWD-S Condenser configuration
AC adapter PFS offset
controller
Side port
Bottom port
Condernser
Dia pillar
illuminator
Filter
slider
Condenser
holder
Lamp
Condenser
turret
Lamphouse
Dia pillar
illuminator
Condenser
cassette
Condenser
lens
Condenser
holder
Filter
slider
Lamp
1
5
5
2
3
3
4
5
10
10
10
5
6
6
7
8
9
9
10
10
11 14 15
13
12
Figure 4-1 Assembly diagram
Chapter 4 Assembly

92
1

Installing the microscope body (microscope base)

Caution

Notes on installation
The microscope base is heavy. Be sure to move the microscope in a group of two or more
people.
When lifting the microscope base, hold it securely by the recess at the bottom front and the
carrying handle at the rear.

Install the microscope in an appropriate location.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET

Figure 4-2 Microscope body installation

Coarse
Fine
ExFine
Obj.
ON/ OFF
6V30W
MAX. MIN.
12V100W

Figure 4-3 Clamping bolt
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET

Figure 4-4 Carrying handle removal

1. Select a location for the installation.
For the installation location, refer to Installation
location and storage location in Notes on
Handling the Product at the beginning of this
manual.
2. Take out the main body (microscope base)
from the box and place it on a stable surface.
3. Remove the clamping bolt.
A clamping bolt is attached to the left side of the
body to secure the product during transportation.
Remove the clamping bolt with the provided 4
mm hex screwdriver, and attach the provided
rubber cap to the clamping bolt hole.
If power is turned on with the clamping bolt
left attached, it will prevent the observation
from being performed, and may also cause
product damage. Be sure to remove the
clamping bolt before turning on the power.

4. Remove the carrying handle by loosening the
two hex socket head bolts with the provided
4 mm hex screwdriver.

Microscope body
(microscope base)
Clamping bolt (M5, x1)

Hexagonal
socket head bolts
(M5, x2)
Carrying handle
Bottom front recession
Chapter 4 Assembly

93
2

Attaching the eyepiece base unit

(1) Attaching TI-T-B, TI-T-BS
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET
Figure 4-5
Eyepiece Base Unit attachment (TI-T-B, TI-T-BS)

1. Place the eyepiece base unit onto the front
part of the microscope base, so that the
eyepiece tube mount of the eyepiece base
unit faces the front.
There are two positioning pins on the bottom of
the eyepiece base unit. Align these pins with the
holes on the microscope base.
2. Secure the eyepiece base unit by tightening
four hex socket head bolts with the provided

(2) Attaching the TI-T-BPH External Phase Contrast Eyepiece Base Unit
To perform external phase contrast microscopy, attach an external phase contrast eyepiece base unit onto
the microscope base, and attach a phase plate suitable for the selected condenser lens and objective to the
eyepiece base unit.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET
Figure 4-6
External phase contrast eyepiece base unit attachment

1. Place the TI-T-BPH External Phase Contrast
Eyepiece Base Unit onto the front part of the
microscope base.
There are two positioning pins on the bottom of
the eyepiece base unit. Align these pins with the
holes on the microscope base.
2. Secure the eyepiece base unit by tightening
four hex socket head bolts with the provided

Selecting objectives and phase plates
The phase plate must be selected to suit the objective being used. Refer to the following table to select an
objective and an appropriate phase plate. Up to three phase contrast plates (A, B, and C) can be attached to
an external phase contrast eyepiece base unit.
Table 4-1

Condenser phase contrast code
(Supported condenser lens)
Objective
External phase
contrast ring
1 Ph3 (LWD, CLWD) P Apo 60x WI (NA1.2) 60x/Ph3
2 Ph3 (LWD, CLWD) P Apo VC 60x WI (NA1.2) 60x/Ph3
5 Ph4 (CLWD) P Apo TIRF 60x H (NA1.45) 60x/Ph4
Eyepiece base unit
Positioning holes (x2)
External phase contrast
eyepiece base unit
Positioning holes (x2)
Hexagonal socket head bolts
(Built into the eyepiece
base unit) (M5, x4)
Hexagonal socket head bolts
(Built into the eyepiece
base unit) (M5, x4)
Chapter 4 Assembly

94
Attaching and replacing phase plates

Figure 4-7 Special tool and phase plate

Figure 4-8 Attaching/replacing the phase plate

Phase plate attachment
A special tool is included with TI-T-BPH Eyepiece
Base Unit. Use this special tool when attaching or
replacing the phase plate.
1. Align the holes on the phase plate to the two
pins on the end of the special tool, and attach
the phase plate to the tool.
The phase plate will be attached magnetically to
the special tool.
2. Select position A, B, or C by rotating the
turret on the external phase contrast
eyepiece base unit.
3. Insert the special tool and the phase plate
into the optical path hole on the top of the
eyepiece base unit. Screw in the phase plate
into the socket on the turret.
4. Pull out the special tool. Affix the provided
sticker to the dedicated position on the front
of the turret.
5. Repeat steps 1 thru 4 for positions A thru C,
as necessary.

Phase plate replacement
1. Select position A, B, or C by rotating the
turret on the external phase contrast
eyepiece base unit.
2. Insert the special tool into the optical path
hole on the eyepiece base unit. Align the two
pins on the special tool to the holes on the
phase plate.
3. Remove the phase plate from the socket by
rotating the tool counterclockwise.
4. Remove the phase plate by pulling out the
special tool.
The phase plate is attached magnetically to the
special tool, and can be retrieved by lifting the
tool.
5. Attach other phase plate as described above
in Phase plate attachment.

Special tool
Phase plate
Special tool
Phase plate
Turret
Sticker position
Chapter 4 Assembly

95
3

Attaching the eyepiece tube and eyepieces

Attach the eyepiece tube to the eyepiece base unit, and the eyepieces to the binocular part of the eyepiece
tube.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET
B

Figure 4-9 Eyepiece tube attachment

B

Figure 4-10 Eyepiece attachment

1. Using a 2 mm hex screwdriver, loosen the
eyepiece tube clamp screw at the
observation port on the front of the eyepiece
base unit.
2. Mount the eyepiece tube onto the eyepiece
base unit and insert the male circular dovetail
joint on the bottom of the eyepiece tube into
the female circular dovetail joint on the
eyepiece base unit.
3. Tighten the eyepiece tube clamp screw with a
2 mm hex screwdriver. Check that the
eyepiece tube and the eyepiece base unit are
fixed securely.
4. Attach the eyepieces to the binocular part of
the eyepiece tube.
When inserting, align any one of the three
grooves on the eyepiece to the protrusion on the
eyepiece tube sleeve. Select the same
magnification for both left and right eyepieces.
To use rubber eye guards, attach them to the
eyepieces.

Eyepiece tube
Dovetail joint
Eyepiece tube
clamp screw
Protrusion on eyepiece tube sleeve
Eyepiece
Binocular part
Chapter 4 Assembly

96
4

Attaching the nosepiece

Attach the nosepiece to the rectangular groove on the focusing part at the center of the microscope base.

(1) Attaching the PFS Motorized Nosepiece
When using an EPI-FL filter turret, attach the Protection Plate (for PFS6 Nosepiece) supplied with the
TI-ND6-PFS Perfect Focus Unit between the EPI-FL filter turret and the nosepiece. For details, refer to
(3) Attaching the protection plate (when using an EPI-FL filter turret).

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse
Fine
ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 4-11 PFS Motorized Nosepiece attachment

Figure 4-12
Fixing the PFS Motorized Nosepiece (top view)
INTERLOCK
PIEZO
PFS
OFFSET

Figure 4-13 PFS connector (right connector panel)

1. Adjust the orientation of the PFS Motorized
Nosepiece, and place it on the rectangular
groove on the focusing part at the center of
the microscope base.
2. Press the PFS Motorized Nosepiece from the
front to the rear, and secure it by tightening
the two M5 hex socket head bolts on the
nosepiece.
3. Guide the cable from the PFS Motorized
Nosepiece through the groove on the
microscope base, to the back of the
microscope.
Be sure to guide the cable through the groove on
the microscope base, so that it does not interfere
with the operation of the microscope.
4. Connect the cable from the PFS Motorized
Nosepiece to the PFS connector on the side
of the microscope.

Do not attach the objectives until the stage has
been attached.

Hexagonal socket
head bolts (M5, x2)

Secure nosepiece by tightening bolts
while pushing in the direction of the arrow.
Eject to rear
via groove on
microscope
base.
PFS Motorized
Nosepiece
Hexagonal socket
head bolts
Chapter 4 Assembly

97
(2) Attaching a manual nosepiece
When using an EPI-FL filter turret, attach the Protection Plate (for the Nosepiece) supplied with Ti-E and
Ti-E/B between an EPI-FL filter turret and the nosepiece. For details, refer to (3) Attaching the protection
plate (when using the EPI-FL filter turret).

L80
EYE
DISPLAY
MEMORY
PFS ON
RECALL
Z-RESET
BRIGHTNESS
R100
L100
FOCUS
Coarse
Fine
ExFine
Obj.
6V30W
MAX.
12V100W
ON/ OFF

Figure 4-14 Nosepiece attachment

Figure 4-15 Fixing the nosepiece (top view)

1. Adjust the orientation of the nosepiece, and
place it on the rectangular groove on the
focusing part at the center of the microscope
base.
2. Press the nosepiece from the front to the
rear, and secure it by tightening the two M5
hex socket head bolts on the nosepiece.

Do not attach the objectives until the stage has
been attached.

Hexagonal socket
head bolts (M5, x2)

Nosepiece
Secure nosepiece by tightening bolts while
pushing in the direction of the arrow.
Nosepiece
Hexagonal socket
head bolts
Chapter 4 Assembly

98
(3) Attaching the protection plate (when using an EPI-FL filter turret)
When using an EPI-FL filter turret with Ti-E or Ti-E/B, a protection plate must be attached between the
EPI-FL filter turret and the nosepiece. The required protection plate will differ depending on the nosepiece.
Follow the procedures below to attach the correct protection plate.

When using the TI-ND6-PFS PFS Motorized Nosepiece
The Protection Plate (for the PFS6 Nosepiece) supplied with the TI-ND6-PFS Perfect Focus Unit covers the
gap between the PFS Motorized Nosepiece and the EPI-FL filter turret (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ),
so as to prevent injury caused by your hands and fingers getting caught.
When using an EPI-FL filter turret, attach the Protection Plate (for the PFS6 Nosepiece) before attaching the
PFS Motorized Nosepiece, as described below.

Figure 4-16
Protection Plate (for the PFS6 Nosepiece) attachment

1. Using a 2 mm hex screwdriver provided with
Ti-E or Ti-E/B, remove the four M3 hex socket
head bolts from the top of the EPI-FL filter
turret, as shown in the figure at left.
2. Attach the Protection Plate (for the PFS6
Nosepiece) onto the top of the EPI-FL filter
turret. Insert and tighten the four M3 hex
socket head bolts that were removed in step
1.
3. Pass the connection cable from the PFS
Motorized Nosepiece through the cable
through-hole on the protection plate. Using
the 4 mm hex screwdriver provided with Ti-E
or Ti-E/B, secure the nosepiece to the
microscope.
For details on attaching the PFS Motorized
Nosepiece, refer to (1) Attaching the PFS
Motorized Nosepiece.

Hexagonal socket
head bolts (M5, x2)
EPI-FL filter turret
Protection Plate
(for the PFS6
Nosepiece)
Hexagonal socket
head bolts
(M3, x4)
Cable
through-hole
Chapter 4 Assembly

99
When using the TI-N6, TI-ND6, or TI-ND6-E nosepiece
The Protection Plate (for the Nosepiece) included with the Ti-E or Ti-E/B microscope covers the gap between
the nosepiece (TI-N6, TI-ND6, or TI-ND6-E) and the EPI-FL filter turret (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ),
so as to prevent injury caused by your hands and fingers getting caught.
When using an EPI-FL filter turret, attach the Protection Plate (for the Nosepiece) before attaching the
nosepiece, as described below.

Figure 4-17
Protection Plate (for the Nosepiece) attachment

1. Using a 2 mm hex screwdriver, remove two of
four M3 hex socket head bolts from the top of
the EPI-FL filter turret (TI-FLC, TI-FLC-E, or
TI-FLC-E/HQ), as shown in the figure at left.
2. Attach the Protection Plate (for the
Nosepiece) onto the top of the EPI-FL filter
turret. Insert and tighten the two M3 hex
socket head bolts that were removed in step
1.
3. Attach the nosepiece (TI-N6, TI-ND6, or
TI-ND6-E) to the Ti-E or Ti-E/B microscope
body, and secure with the 4 mm hex
screwdriver.
For details on attaching the nosepiece, refer to
(2) Attaching a manual nosepiece.
When using the TI-ND6-E motorized nosepiece,
refer to Motorized Units for Ti Series
Instructions.

Hexagonal socket
head bolts (M5, x2)
Nosepiece
EPI-FL filter turret
Protection Plate
(for the Nosepiece)
Hexagonal socket
head bolts (M3, x2)
Chapter 4 Assembly

100
5

Attaching the dia pillar illuminator

Attach the dia pillar illuminator to the microscope base.
The Ti series microscopes can be used with two types of dia pillar illuminators (100W and 30W).

(1) Attaching the TI-DH Dia Pillar Illuminator 100W
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET

Figure 4-18 Dia Pillar Illuminator 100W attachment

Figure 4-19 Condenser mount attachment

Attach TI-DH Dia Pillar Illuminator 100W to the
microscope base.
When working, hold the dia pillar illuminator
to prevent it from falling.

1. Mount the dia pillar illuminator onto the
microscope base.
A positioning pin is provided on the microscope
base. Align the pinhole on the dia pillar
illuminator to the positioning pin.
2. Using the 4 mm hex screwdriver, secure the
dia pillar illuminator by tightening the four M5
hex socket head screws provided with the dia
pillar illuminator.
3. Attach the condenser mount to the dia pillar
illuminator.
(1) Remove the fall-stop screw.
(2) Attach the condenser mount by sliding it onto
the dovetail groove on the dia pillar
illuminator.
Slide the mount upward to the limit.
(3) Using a hex screwdriver, securely tighten the
clamp screw on the right of the condenser
mount.
(4) Attach the fall-stop screw.
(5) When using a condenser lens other than
ELWD or ELWD-S, loosen the clamp screw
on the condenser mount and slide the
condenser mount downward to the fall-stop
screw, and then tighten the clamp screw
securely in this position.

Dia Pillar
Illuminator
100W
Hexagonal socket
head bolts (M5, x4)
Clamp screw
Condenser
mount
Fall-stop screw
Chapter 4 Assembly

101

Figure 4-20 Lamphouse attachment

Figure 4-21 Fixing the cable

Figure 4-22 Ferrite core attachment

4. Attach the lamphouse to the dia pillar
illuminator.
CAUTION
HOT - Do not touch the lamp and the
lamphouse while the lamp is on or for
thirty minutes after it has been turned
off.

(1) Insert the lamphouse into the lamphouse
mount at the top of the dia pillar illuminator.
Align the positioning pin on the dia pillar
illuminator with the groove on the cylinder of
the lamphouse.
(2) Insert a hex screwdriver into the hole on the
top of the dia pillar illuminator. Secure the
lamphouse by tightening the lamphouse
clamp screw.
(3) Secure the lamphouse cable with the cable
clamp on the back of the dia pillar illuminator.
The cable clamp is attached to the dia pillar
illuminator with the hooks on its sides. To
remove the cable clamp, push in the hooks
from the sides.
The cable clamp can hold up to four cables.
5. Attach the provided ferrite core on the
connector end of the lamp cable.
(1) Snap open the ferrite core.
(2) Wrap the lamp cable once around the ferrite
core, so that the cable passes through the
ferrite core twice.
Adjust the position of the ferrite core so
that it is approximately 10 mm from the
lamp cable connector.
(3) Close the ferrite core.

For details on connecting the lamphouse, the power
supply device, and Ti-E or Ti-E/B, refer to 13.
Connecting the power supply.

Lamphouse
clamp screw
Cable clamp
Insert hex wrench and
tighten clamp screw.
10 mm
Ferrite core
The lamp cable is
passed twice in
the ferrite core.
Connector
Chapter 4 Assembly

102
(2) Attaching the TI-DS Dia Pillar Illuminator 30W
EN
TI - DS 30W
6V 30W
HALOGEN
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
TERLOCK
PIEZO
PFS OFFSET
B

Figure 4-23 Dia Pillar Illuminator 30W attachment

Figure 4-24 Ferrite core attachment

Attach TI-DS Dia Pillar Illuminator 30W to the
microscope base.
When working, hold the dia pillar illuminator
to prevent it from falling.

1. Mount the dia pillar illuminator onto the
microscope base.
A positioning pin is provided on the microscope
base. Align the pinhole on the dia pillar
illuminator to the positioning pin.
2. Using the 4 mm hex screwdriver, secure the
dia pillar illuminator by tightening the four M5
hex socket head bolts provided with the dia
pillar illuminator.
3. Attach the provided ferrite core on the pillar
end of the lamp cable.
(1) Snap open the ferrite core.
(2) Wrap the lamp cable once around the ferrite
core, so that the cable passes through the
ferrite core twice.
Adjust the position of the ferrite core so that
it is approximately 20 to 30 mm from the
pillar.
(3) Close the ferrite core.

For details on connecting the lamphouse, the power
supply device, and Ti-E or Ti-E/B, refer to 13.
Connecting the power supply.

Dia Pillar
Illuminator
30W
Hexagonal socket
head bolts (M5, x4)
20 to 30 mm
Back of Dia Pillar
Illuminator 30W
The lamp cable is
passed twice in
the ferrite core.
Ferrite core
Chapter 4 Assembly

103
6

Attaching the filter slider

Attached the desired filter to the filter slider, and
attach the filter slider to the slot of the dia-illuminator.
Do not touch filters or other optical components
with your bare hands.

Although the filter slider for the 30W dia-illuminator
has a different shape, the method for securing the
filters is the same.
Figure 4-25 Attaching filters

Figure 4-26 Inserting filter sliders
(for Dia Pillar Illuminator 100W)

OPEN
TI - DS 30W
6V 30W
HALOGEN

Figure 4-27 Inserting filter sliders
(for Dia Pillar Illuminator 30W)

1. Attach the desired filter to the filter slider.
Attach it from the back of the filter slider. The
mounting hole has three tabs to keep the filter
from falling. Only one tab can be moved to the
side. Move this tab aside, and attach the filter.
2. Affix a label indicating the filter type on the
tab of the filter slider.
3. Insert the filter slider into the slot of the dia
pillar illuminator.
The filter slider has latches that determine the
limit of slide operation. Press the latches up, and
press the filter into the slot.
You can insert up to four filter sliders for 100W
type illuminator and three filter sliders for 30W
type illuminator.
You can insert filters sliders from the right or the
left. If they are all inserted from the same
direction, they will be difficult to handle, so you
should insert them alternately from the left and
right.
To maintain uniformity with the Dia Pillar
Illuminator 100W, install the diffusion filter (filter
slider D) into the slot nearest to the lamphouse.

Removing a filter slider
Latches at both ends of the filter slider are at their
end point when sliding. When removing a filter slider,
you can slide it out by pushing the latch on the
opposite side up with your finger to release the filter
slider. Applying undue force on the filter slider can
break the latches.

Filter
Filter slider (back)
Latches
Latch
Tab
Chapter 4 Assembly

104
7

Attaching the stage

Attach the stage to the microscope base.
If objectives are attached to the nosepiece,
remove them before attaching the stage.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET

Figure 4-28 Stage mounts

1. Place the stage onto the mounts at the base
of the eyepiece base unit and at the base of
the dia pillar illuminator.
Positioning pins are provided on the mount at the
base of the dia pillar illuminator. Align the holes
on the bottom of the stage to these pins.
Stages with a stage movement knob are typically
attached with the knob positioned in the far right.
However, they can also be rotated by 180 degrees
and be attached with the knob in the near left.

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET

Figure 4-29 Stage attachment

2. Secure the stage by tightening the four M5
hex socket head bolts provided with the
stage.
Be sure to use spring washers and washers with
the hex socket head bolts.

Mount Mount
Hexagonal socket
head bolts (M5, x4)
Washers
Spring
washers
Stage
Chapter 4 Assembly

105
8

Attaching objectives

Attach objectives to the nosepiece.
If the stage has not been attached, first attach the
stage.

B
Coarse
Fine
ExFine
Epi S Refocus L Block
Escape

Figure 4-30 Objective attachment

1. Remove the concentric ring, specimen
holder, and other equipment from the stage.
2. Screw in an objective into a socket on the
nosepiece through the opening in the stage.
Attach the objectives so that magnification is
increased by rotating the nosepiece clockwise
(as viewed from above).

Nosepiece
Objective
Chapter 4 Assembly

106
9

Replacing the dia illumination lamp

Caution

The lamp, the dia pillar illuminator, and the power supply must be used in specific
combinations. See page 56 to select the correct combination of the devices. Be sure to use
the specified lamps.
When replacing the lamp, turn off the power switch and unplug the power cord.
The lamp and its surroundings will be hot while the lamp is on and immediately after it is
turned off. When replacing the lamp, wait approximately 30 minutes after turning off the
lamp, and make sure that the lamp has cooled sufficiently before working.
Do not touch the lamp glass with your bare hands. Fingerprints and other dirt on the lamp
may result in uneven illumination and reduce the service life of the lamp. Wear gloves when
handling the lamp.
Securely close the lamphouse cover after replacing the lamp. Never turn on the lamp with
the cover removed.

(1) For TI-DH Dia Pillar Illuminator 100W

Figure 4-31 Lamphouse Cover Removal

Figure 4-32 Lamp Replacement

CAUTION
thirty minutes after it has been turned off.

1. Insert a hex wrench into the hole on the top of
the lamphouse cover. Loosen the clamp
screw and remove the lamphouse cover.
2. Push in the lamp clamp lever and remove the
used lamp from the socket.
3. Insert a new lamp into the socket.
While pushing in the lamp clamp lever, push the
lamp electrodes (pins) into the pinhole in the
socket. Insert the lamp to the limit, and release
the lamp clamp lever.
Be sure to use the specified lamp.
Do not touch the lamp glass with your bare
hands.
Make sure the lamp is not tilted when the lamp
clamp lever is released.
4. Re-attach the lamphouse cover, and secure it
by tightening the clamp screw.

Clamp screw
Lamphouse
cover
Insert hex wrench and loosen clamp screw.
Remove the lamphouse cover. (Clamp
screw remains attached to the cover.)
Lamp clamp
lever
Lamp
Push in lamp clamp lever to open pin hole on socket.
Remove used lamp while pushing in lever. Attach new lamp.
Chapter 4 Assembly

107
(2) For TI-DS Dia Pillar Illuminator 30W

OPEN
TI - DS 30W 6V
30W HALOGEN

Figure 4-33 Rear cover of the lamphouse

Figure 4-34 Replacing lamps

CAUTION
thirty minutes after it has been turned off.

1. Loosen the locking screw on the back of the
dia pillar illuminator, and remove the limit
plate from the rear cover.
2. Open the rear cover on the back of the dia
pillar illuminator by lifting it backward.
3. Remove the used lamp from the socket.
4. Insert a new lamp into the socket.
Be sure to use the specified lamp.
Do not touch the lamp glass with your bare
hands.
5. Close the rear cover.
6. Re-attach the limit plate onto the rear cover,
and secure it by tightening the locking screw.

Rear cover
Limit plate
Locking screw
Rear cover
Lamp
Chapter 4 Assembly

108
10

Attaching the condenser

(1) Attaching to TI-DH Dia Pillar Illuminator 100W

The following condensers can be attached to TI-DH
Dia Pillar Illuminator 100W:
TI-C Condenser Turret (system condenser)
ELWD-S Condenser
High NA Condenser

Preparation for attachment

Figure 4-35 Condenser Holder

Using a hex screwdriver, loosen the condenser
clamp screw on the right side of the condenser
holder.
* The condenser clamp screw is located inside the
hole on the right side of the condenser holder. If
the condenser mount is shifted from the reference
position, the screw will not be visible in the hole.
In that case, loosen the condenser mount rotation
clamp screw, align the positioning groove on the
mount with the positioning pin on the condenser
holder by rotating the mount, and then tighten the
mount rotation clamp.

Attaching the system condenser

Figure 4-36 System condenser attachment

Figure 4-37 Condenser cassette and
condenser lens attachment

1. Rotate the condenser turret to the A
position (vacant hole cassette position for
bright-field microscopy).
2. With the A label facing the front (towards
yourself), insert the circular dovetail joint on
the system condenser into the bottom of the
condenser holder, and secure it by tightening
the condenser clamp screw.
Attach the condenser turret by sliding it in from
the front.
3. Insert the condenser cassette into the
condenser turret, and secure it with two hex
socket head screws.
Up to five condenser cassettes can be attached
to the turret. Attach the cassettes so that their
numbering is increased by rotating the turret
clockwise (as viewed from above).
4. Screw in the condenser lens into the bottom
of the turret.

For combinations of condenser lenses and
condenser cassettes, see Chapter 3, Operation.

Positioning
pin
Positioning
groove
Mount rotation
clamp screw
Condenser
mount
Condenser
holder
Condenser
clamp screw*
(within side hole)
Condenser
clamp screw
Dovetail joint
System condenser Position A
Condenser
cassette
Condenser lens
Chapter 4 Assembly

109

Attaching the ELWD-S condenser

Figure 4-38 ELWD-S Condenser attachment

With the indication on the turret facing the front
(towards yourself), insert the condenser turret into
the bottom of the condenser holder, and secure it by
tightening the condenser clamp screw.

Attaching the high NA condenser

Figure 4-39 High NA condenser attachment

1. Insert the extension tube provided with the
high NA common condenser lens into the
condenser holder. The Nikon nameplate must
face the front (near side). Tighten the
condenser clamp screw. (The extension tube
can be attached to the condenser holder after
the high NA condenser holder is attached to the
extension tube.)
2. Attach the high NA condenser holder to the
lower part of the extension tube attached in
Step 1. The aperture diaphragm lever must
face the front (near side). Tighten the
condenser clamp screw to fix the high NA
condenser holder.
3. Screw in the high NA common condenser
lens to the lower part of the field diaphragm
knob of the dia pillar illuminator.

Condenser
clamp screw
Dovetail joint
ELWD-S Condenser
Condenser
display
High NA condenser
holder clamp screw
High NA common
condenser lens
Condenser cassette
Extension tube (provided
with the high NA common
condenser lens)
High NA condenser holder
Condenser cassette
Aperture diaphragm
lever position
Condenser
clamp screw
Nikon nameplate
position
Chapter 4 Assembly

110
(2) Attaching to TI-DS Dia Pillar Illuminator 30W

The following condensers can be attached to TI-DS
Dia Pillar Illuminator 30W:
ELWD-S Condenser
HMC Condenser

Attaching the ELWD-S condenser

EN
TI - DS 30W
6V
30W
HALOGEN

Figure 4-40 ELWD-S Condenser attachment

1. Secure the condenser holder to the dia pillar
illuminator.
2. Attach the ELWD-S condenser to the
condenser holder.

Attaching the HMC condenser

OPEN
TI - DS 30W
6V 30W
HALOGEN

Figure 4-41 HMC Condenser attachment
(system condenser)

1. Attach the 52 mm polarization filter to the
extension tube provided with the HMC
condenser lens.
Threads are cut on the inside of the extension
tube and on the outside of the polarization filter.
2. Attach the extension tube to the dia pillar
illuminator.
3. Attach the condenser holder to the extension
tube.
4. Attach the turret on the system condenser to
the condenser holder.
5. Screw in the HMC condenser lens into the
turret.
6. Attach the HMC condenser cassette to the
turret.

Condenser holder
ELWD-S Condenser
Extension tube
System condenser
turret
HMC condenser
lens Condenser cassette
52 mm polarization filter
Condenser holder
Chapter 4 Assembly

111
11

Attaching the side port

O
O

Figure 4-42 Side port adapter attachment

1. Loosen the two side port adapter clamp
screws and remove the plastic cap from the
side port.
2. Insert the side port adapter into the side port,
and secure it by tightening the side port
adapter clamp screws.
3. Attach adapters to the camera device.
4. Insert the adapters and the camera device
into the side port adapter, and secure them
by tightening the two camera adapter clamp
screws.
Camera devices require compatible adapters
for attachment. First attach the adapters to the
camera device, and then attach the camera
device with the adapters into the side port
adapter.
Attach a protective cap onto the port when the
port is not in use.

Example: Attaching the C mount TV camera to the direct C mount adapter
(1) Screw the C mount TV camera securely into the direct C mount adapter.
(2) Insert the direct C mount adapter into the side port adapter, and secure it by tightening the locking
screw.
When detaching photomicrographic equipment, hold it steady, and then loosen the locking
screw.
If the locking screw is loosened without holding the equipment, the equipment may drop. To
avoid dropping photomicrographic equipment, make sure you have a firm grip before
loosening the screw.

Side port adapter
clamp screws (x2)
Side port adapter
Camera adapter
clamp screws
(x2)
Chapter 4 Assembly

112
12

Attaching the bottom port (Ti-E/B only)

Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
INTERLOCK PIEZO
PFS
OFFSET

Figure 4-43 Bottom port adapter attachment

1. Loosen the two bottom port adapter clamp
screws and remove the metal cap from the
bottom port.
2. Insert the bottom port adapter into the
bottom port, and secure it by tightening the
two bottom port adapter clamp screws.
3. Attach adapters to the camera device.
4. Insert the adapters and the camera device
into the bottom port adapter, and secure
them by tightening the two camera adapter
clamp screws.
Camera devices require compatible adapters
for attachment. First attach the adapters to the
camera device, and then attach the camera
device with the adapters into the bottom port
adapter.
Attach a protective cap onto the port when the
port is not in use.

Example: Attaching the C mount TV camera to the direct C mount adapter
(1) Screw the C mount TV camera securely into the direct C mount adapter.
(2) Insert the direct C mount adapter into the bottom port adapter, and secure it by tightening the
locking screw.
When detaching photomicrographic equipment, hold it steady, and then loosen the locking
screw.
If the locking screw is loosened without holding the equipment, the equipment may drop. To
avoid dropping photomicrographic equipment, make sure you have a firm grip before
loosening the screw.

Bottom port
adapter
Bottom port adapter
clamp screws (x2)
Camera adapter
clamp screws (x2)
Chapter 4 Assembly

113
13

Connecting the power supply

A power supply is required to turn on the dia
illumination lamp. Check that the POWER switch on
the power supply is turned off (pressed on the O
side), and then connect the power supply as
described below.

Figure 4-44 Power supply

(1) Lamp cable
Connect the lamp cable from the dia pillar illuminator
to the DC output connector on the power supply. A
lock ring is provided on the lamp cable connector.
Secure the lamp cable with the lock ring.
For Dia Pillar Illuminator 100W
Connect the lamp cable from the D-LH/LC
lamphouse to the 12 VDC output connector on the
TI-PS100W Power Supply.
Before connecting the cable, check that the ferrite
core is properly attached on the connector end of the
lamphouse cable (see page 101).
For Dia Pillar Illuminator 30W
Connect the lamp cable from the dia pillar illuminator
to the 6 VDC output connector on the
TE-PS30W/TE-PSE30 Power Supply A.
Before connecting the cable, check that the ferrite
core is properly attached on the pillar end of the
lamphouse cable (see page 102).

MIC CONTROL
POWER
OFF ON
LAMP CTRL
DC24V IN
USB
5 3 1 0 0 1
(2)

Figure 4-45 Ti-E, Ti-E/B (rear view)

(2) Control cable
Connect one end of the control cable to the LAMP
CTRL connector on the rear of the microscope base,
and the other end to the EXTERNAL connector on
the TI-PS100W Power Supply or the CTRL
connector on the TE-PS30W/TE-PSE30 Power
Supply A.

(3) Power cord
Connect the plug end of the power cord to the wall
outlet, and the other end to the AC inlet connector on
the power supply.
To prevent electric shock, do not connect the
power cord until all other assembly
procedures are completed.

EXTERNAL/CTRL switch
12VDC/6VDC
output connector
AC inlet
EXTERNAL/CTRL
connector
POWER
switch
LAMP CTRL connector
Chapter 4 Assembly

114
14

Connecting the PFS Offset Controller

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
PFS
D O W N
UP PFS
OFFSET
CONTROLLER
FINE
COARSE
INTERLOCK
PIEZO
PFS
OFFSET

Figure 4-46 PFS Offset Controller connection

When using the PFS Motorized Nosepiece,
connect the PFS Offset Controller to the Ti-E or
Ti-E/B microscope body.
Connect the cable from the PFS Offset Controller to
the OFFSET connector on the right side of the
microscope body.

PFS Offset
Controller
Chapter 4 Assembly

115
15

Connecting the AC adapter

Caution

Use the AC adapter included with the product.
Use a power cord specified in Chapter 7, Specifications.
To prevent electric shock, do not connect the power cord until all other assembly
procedures are completed.

Figure 4-47 AC adapter
MIC CONTROL
POWER
OFF ON
LAMP CTRL
DC24V IN
USB
5 3 1 0 0 1
(2)

Figure 4-48 Ti-E, Ti-E/B (rear view)

Power to the microscope is supplied via an AC
adapter.
The AC adapter can be used with 100 to 240 VAC at
50-60Hz, and can be used with most wall outlets in
the world.
1. Check that the POWER switch on the Ti-E or
Ti-E/B is turned off (the POWER switch is set
to the OFF side).
2. Connect the DC output cable from the AC
adapter to the DC24V IN connector on the
rear connector panel of the microscope.
3. Connect the AC adapter to a wall outlet with a
specified power cord.

This is the end of the standard system assembly. Refer to the figure on page 118 to check the
assembly result.
When using the TI-ND6-PFS Perfect Focus Unit, proceed to 16. Registering objectives to register
the objectives attached to the microscope.

AC inlet
AC adapter
DC output
cable
DC24V IN connector POWER switch
Chapter 4 Assembly

116
16

Registering objectives

Caution

Notes on registering objectives
To register objectives, connect the microscope to a PC, and turn on their power.
Check that all assembly and connection procedures are complete before proceeding.

To use the PFS Motorized Nosepiece, you will need to register the objective information on the microscope.
This section describes how to register the objective information on the Ti-E or Ti-E/B microscope body.
When using TI-HUBC/A Hub Controller A and TI-RCP Remote Control Pad, the objective information can
be registered from the remote control pad. For details, refer to the instruction manual provided with the
TI-RCP Remote Control Pad.

(1) Preparing the software
To register the objective information on the microscope, install the following software on a commercial PC.

Nikon Ti-Control(Ti Setup Tool)
The Ti Control software and its instruction manual can be downloaded from the following website.
http://www.nikon-instruments.jp/eng/service/download/DLList.aspx?CID=3
User registration is required to download the software.
For details on using the software, refer to its instruction manual.

For details on using the software and setting up the microscope, refer to the online help and instruction
manual.

Chapter 4 Assembly

117
(2) Registering the objective information

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS

Figure 4-49 Connecting microscope and PC
(example)

Figure 4-50 Ti Control startup screen

1. Using a standard USB cable, connect the
USB connector on the microscope to the
USB connector on the PC.
2. Install the registration software onto the PC.
Install Ti Control. For the software installation
details, refer to the instruction manual of the
software.
3. Start the installed software, and register the
information for the objectives attached to the
PFS Motorized Nosepiece.
Information for PFS-compatible objectives is
pre-registered on the software. For each socket
on the nosepiece, specify the objective to be
used.
4. Save the registered information.
The settings are stored on the built-in memory of
the microscope.
5. Exit the software, and disconnect the USB
cable from the PC and the microscope.

A list of PFS-compatible objectives (as of January
2008) are provided on page 128. To register
future objectives with PFS support, contact Nikon.

USB cable
(Connect to USB
cable on back of
microscope.)
PC
Chapter 4 Assembly

118
System Configuration
The illustrations below show the Ti-E microscope body with the following accessories:
TI-DH Dia Pillar Illuminator 100W, TI-C Condenser Turret, D-LH/LC Precentered Lamphouse LC, 12 V 100
W halogen lamp, TI-PS100W Power Supply, TI-SR Rectangular Mechanical Stage, TI-T-B Eyepiece Base
Unit, TI-TD Eyepiece Tube B, CFI 10x eyepieces, TI-ND6-PFS Perfect Focus Unit, objectives, etc.

B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X 1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
P
F
S
D O W
N
U
P
P
F
S
OFFSET
CONTROLLER
FINE
COARSE

Figure 4-51 Ti-E

Dia illumination lamphouse
(Figure: D-LH/LC)
Filter sliders
(both sides, 4 total)
Dia pillar illuminator
(Figure: TI-DH)
Condenser mount
Nosepiece (Figure: TI-ND6-PFS)
Stage knob
Right side port
Focus knob
Power supply
(Figure: TI-PS100W)
Eyepiece base unit
(Figure: TI-T-B)
Eyepiece tube (Figure: TI-TD)
Eyepieces (both sides)
Condenser lens
Condenser turret (Figure: TI-C)
Condenser centering knob
Aperture diaphragm open/close lever
(both sides)
Objectives
Stage ring (concentric ring)
Microscope base
(Figure: Ti-E)
Stage (Figure: TI-SR)

119
Troubleshooting
5

Misuse of the product may result in poor product performance, even if the product is properly functional. If
you experience any of the following problems, check the following table for possible causes before
requesting service.
If the problem is not listed below, or if the problem cannot be resolved by the suggested countermeasure,
unplug the power cord and contact Nikon.
5.1 Image Viewing
Problem Possible cause Countermeasure
Incorrectly attached part. Attach all parts correctly.
Movable part in inappropriate position.
The following parts must be moved to
click-stop positions: optical path selector
knob, nosepiece, filter sliders, condenser
turret, and Bertrand lens in/out lever.
Interference of the concentric ring on the
stage with the optical path.
Move the specimen.
Field diaphragm image not focused on
focal plane of specimen.
Focus and center the condenser
correctly.
Opening of the field diaphragm too
narrow.
Open the field diaphragm to be slightly
larger than the field of view.
Vignetted field of view.
No visible image.
Uneven brightness in field
of view.
Image too dark.
Dirt or dust on the lens or other optical
element, or on the culture vessel.
Clean the optical elements.
Use a clean culture vessel.
Dirt or dust is seen in
the field of view.
correctly.
Objective correction ring not matched to
culture vessels bottom plate thickness.
Use an appropriate correction ring.
Culture vessels bottom plate thickness is
out of the objectives correction range.
Use a culture vessel with bottom plate
thickness within the correction range.
Poor image quality.
Poor contrast or
resolution.
correctly.
Bright-field objective used. Use a phase contrast objective.
Condenser annular diaphragm not in the
optical path.
Move an annular diaphragm with the
same Ph code as the phase contrast
objective into the optical path.
Annular diaphragm not centered. Center the annular diaphragm.
Phase contrast effect
cannot be obtained in
phase contrast
microscopy.
Aperture diaphragm of system condenser
not fully open.
Fully open the diaphragm.
Chapter 5 Troubleshooting
5.1 Image Viewing
120
Uneven focus.
Nosepiece attached incorrectly. Or its
rotation is not stopped at a click-stop
position.
Attach the nosepiece correctly and rotate
it to a click-stop position.
Specimen not level with stage.
Set the specimen onto the stage
correctly.
Nosepiece attached incorrectly. Or its
rotation is not stopped at a click-stop
position.
Attach the nosepiece correctly and rotate
it to a click-stop position.
Annular diaphragm not centered. Center the annular diaphragm.
Drifting image.
Tilted dia pillar illuminator.
Raise the dia pillar illuminator upright
until it reaches the limit.
NCB11 filter not in optical path. Move the ND filter into the optical path.
Yellow-tinged image.
Lamp voltage too low.
Adjust brightness control knob to match
lamp rating.
ND filter not in optical path. Move the ND filter into the optical path.
Field of view too bright.
Lamp voltage too high.
Lower lamp voltage with the brightness
control knob.
Opening of the aperture diaphragm too
narrow.
Set the aperture diaphragm to 70 to 80%
of the numerical aperture of the objective.
correctly.
Insufficient brightness
in field of view.
Optical path not set to the observation
port.
Use the optical path selector switches
and set the optical path to the
observation port.

5.2 Operation
121
5.2 Operation
Image cannot be
focused even with the
objective at the highest
position.
Stage attached incorrectly. Attach the stage correctly.
Image cannot be
focused with 20x or 40x
objective.
Culture vessels bottom plate thickness is
out of the objectives correction range.
Use a culture vessel with bottom plate
thickness within the correction range.
When viewed through
the binocular eyepiece,
the images do not
merge into a single
image.
Interpupillary distance not adjusted. Adjust the interpupillary distance.
Diopters not adjusted. Adjust the diopters.
Eyestrain develops
during observations.
Inappropriate brightness.
Adjust brightness of lamp, or use ND
filters.

5.3 Electrical System
There is no power even
though the power
switch is set to ON.
The AC adapter or the power cord for the
power supply is not connected at all, or is
not connected securely.
Turn off the power switch, and check
connection of AC adapter and power
cord.
The dia illuminator
lamp does not light.
Dead lamp. Replace with a specified lamp.
Dia illuminator lamp
burns out in a short
time.
Unsupported lamp used. Replace with a specified lamp.
Control cable not connected correctly. Check connection of cable. The brightness of the
lamp does not change
even when the
on the microscope is
rotated.
EXTERNAL (or CTRL) switch on the
power supply is turned off.
Turn the switch on.
Dead lamp. Replace with a specified lamp. The lamp does not turn
on/off even when the
dia illumination lamp
ON/OFF switch on the
microscope is
operated.
Control cable not connected correctly. Check connection of cable.
The brightness of the
lamp does not change
even when the
on the power supply is
rotated.
EXTERNAL (or CTRL) switch on the
power supply is turned on.
Turn the switch off.

5.4 Perfect Focus System
122
PFS information is not selected as a
display item.
Press the DISPLAY switch to display PFS
information.
PFS information not
displayed on status
display panel.
PFS Motorized Nosepiece not
connected.
Attach and connect the PFS Motorized
Nosepiece correctly.
The system is detecting the boundary
surface.
(This is normal behavior.)
The system continues to detect the
boundary surface even when PFS is
OFF. The FOCUS indicator will be lit
when the boundary surface is detected.
The indicator can be used to assist in
focusing.
FOCUS indicator lights
up while PFS-ON switch
is OFF.
Registered objective information
incorrect. (Registered information does
not match the objective in use, or
information for a PFS objective is
registered but no objective is attached.)
Register correct objective information.
If no objective is attached, delete the
objective information.
Offset already at limit of adjustable range.
Rotate dial in the opposite direction. The
offset function must be used within the
adjustable range.
Focus position cannot
be moved with the
offset dial.
PFS Offset Controller attached
incorrectly.
Turn off the power. Disconnect and
reconnect the PFS Offset Controller.
MEMORY switch pressed during offset
recall operation.
Retry after offset recall operation is
complete.
Offset cannot be
registered by pressing
the MEMORY switch.
(No beep)
Objective in the optical path does not
support PFS. (Panel display: ER1)
Register the objectives correctly, and
move an appropriate objective into the
optical path.
Offset cannot be
restored by pressing
the RECALL switch.
(No beep)
optical path.
optical path.
PFS does not operate
when the PFS-ON
switch is pressed.
(Six short beeps)
Dichroic mirror in the PFS unit is not in
the optical path. (Panel display: Out)
Move dichroic mirror into the optical path.
No specimen on stage. Set a specimen and focus on it manually.
Objective too far from boundary surface
of specimen.
Rotate focus knobs to move objective
closer to the boundary surface.
DIS appears on the
status display panel.
Shape or material of specimen not
suitable for PFS.
Use a specimen suited for PFS.
PFS will not function if the cover glass is
too thick or if the refraction index of the
solution is too high (n is close to 1.5).
See Supported Specimens on page 83.
123
No specimen on stage. Set a specimen and focus on it manually.
Objective too far from boundary surface
of specimen.
Rotate focus knobs to move objective
closer to the boundary surface.
Focusing operation is
terminated and the
buzzer sounds, approx.
5 seconds after
pressing the PFS-ON
switch.
Shape or material of specimen not
suitable for PFS.
Use a specimen suited for PFS.
PFS will not function if the cover glass is
too thick or if the refraction index of the
solution is too high (n is close to 1.5).
See Supported Specimens on page 84.
Boundary surface not detected within
time limit. (Panel display: ER2)
When using a dry objective, PFS is
turned off for safety if no boundary
surface is detected within 5 seconds.
PFS dichroic mirror not in the optical
path. (Panel display: ER3)
PFS is turned off for safety. Move
dichroic mirror into the optical path and
turn on PFS again.
Nosepiece is placed out of a click-stop
position. (Panel display: ER4)
PFS is turned off for safety. Rotate
nosepiece to a click-stop position and
turn on PFS again.
Limit reached for vertical stage
movement. (Panel display: ER5)
PFS is turned off for safety.
Reflectance of specimen too high.
(Panel display: ER6 or ER7)
Check that a glass-bottomed dish
(No.1S) is used for the specimen.
A long beep sounds
during the perfect focus
operation and the
perfect focus function
ends.
PFS internal error.
(Panel display: ER8 or ER9)
Contact Nikon.

124
Daily Maintenance
6

6.1 Cleaning Optical Components
Do not allow dust, fingerprints, or any other dirt to get on the optical components (i.e. lenses and filters). Dirt
on optical components will degrade the image quality. If an optical component becomes dirty, clean them as
described below.
Remove any dust by brushing off with a soft brush or by wiping gently with gauze.
If and only if there are fingerprints or grease on the optical component, wipe gently with a piece of soft,
clean lens tissue, cotton cloth, or gauze dampened with a small amount of absolute alcohol (ethyl or
methyl).
When removing immersion oil from an objective, use only petroleum benzine. After wiping with petroleum
benzine, wipe with absolute alcohol (ethyl or methyl) for a better finish.
If petroleum benzine is unavailable, use methyl alcohol. However, as methyl alcohol does not clean as
well as petroleum benzine, it will be necessary to wipe a few more times (usually three to four wipes).
Never use petroleum benzine to clean the entrance lens or the prism surface of the eyepiece tube.
Absolute alcohol is highly flammable. Handle with care. Do not use near an open flame, or operate a
power switch in the vicinity.
When using absolute alcohol, follow the instructions provided by the manufacturer.
6.2 Cleaning the Microscope Body
Use silicon cloth to clean the microscope body.
For persistent dirt, dampen a piece of gauze with neutral detergent and wipe gently.
Do not use organic solvents. They may cause discoloration of plastic parts.
6.3 Disinfecting the Microscope
Use a 70% medical alcohol for disinfection.
If a specimen is spilt onto the microscope, check if the specimen is hazardous. If the specimen is
hazardous, follow the standard procedures for your facility.
Do not use organic solvents. The may cause discoloration of plastic parts.
6.4 Storage
Store the product in a dry location where mold is unlikely to grow.
Store the objectives and eyepieces in a desiccator or equivalent, along with some desiccant.
Put a dust-proof cover over the product to protect it from dust.
Before putting on the dust-proof cover, turn off the power switch on the equipment (press the OFF side
or the O side) and wait until the lamp has cooled sufficiently.
6.5 Periodic Inspections (Paid Service)
Periodic inspections (expenses charged) are recommended to maintain the performance of the product. For
details, contact Nikon.

125
Specifications
7

7.1 Microscope (Ti-E or Ti-E/B) with TI-DH Dia Pillar Illuminator 100W
System configuration Microscope, TI-DH Dia Pillar Illuminator 100W, and TI-PS100W Power Supply
Dimensions 260 (W) x 559 (D) x 729 (H) mm
Mass 26.5 kg
Optical system
Objectives: CFI60
Eyepieces: Field number 22
Nosepiece: Six sockets
Mechanical system
Focusing mechanism: Stroke: 9.5 mm
Focus knobs: Coarse focus: 300 m per rotation (reference value)*
Fine focus: 50 m per rotation
Extra fine focus: 6.25 m per rotation
* When set to COARSE mode, the distance traveled by
rotating a focus knob will vary depending on how fast
the knob is rotated.
D-LH/LC
Precentered Lamphouse
LC
Input ratings: 12 VDC, 100 W
Lamp ratings: 12 V 100 W halogen lamp
Specified lamp model: Halogen lamp (OSRAM HLX 64623 or PHILIPS 7724I)
Average lamp life: 2000 hours
TI-PS100W
Power Supply
Input ratings: 100 to 240 VAC (10%), 1.8 A, 50/60 Hz
Built-in fuse ratings: 250 V T4A
Output ratings: 12 VDC, 100 W
Maximum output current: 8.4 A
Electric shock protection class:
Class I
Remarks: UL listed product, GS approved
Operating conditions
Temperature: 0 to +40C
Relative humidity: 85% RH max. with no condensation
Altitude: 2000 m max.
Pollution degree: Degree 2
Installation category: Category II
Indoor use only
Transport and storage
conditions
Temperature: -20 to +60C
Chapter 7 Specifications
7.2 Microscope (Ti-E or Ti-E/B) with TI-DS Dia Pillar Illuminator 30W
126
7.2 Microscope (Ti-E or Ti-E/B) with TI-DS Dia Pillar Illuminator 30W
System configuration
For countries with power supply of 100 to 120 VAC:
Microscope, TI-DS Dia Pillar Illuminator 30W,
and TE-PS30W Power Supply A
For countries with power supply of 230 VAC:
Microscope, TI-DS Dia Pillar Illuminator 30W,
and TE-PSE30 Power Supply A
Dimensions 260 (W) x 497 (D) x 619 (H) mm
Mass 22.5 kg
Optical system
Objectives: CFI60
Eyepieces: Field number 22
Nosepiece: Six sockets
Mechanical system
Focusing mechanism: Stroke: 9.5 mm
Focus knobs: Coarse focus: 300 m per rotation (reference value)*
Fine focus: 50 m per rotation
Extra fine focus: 6.25 m per rotation
* When set to COARSE mode, the distance traveled by
rotating a focus knob will vary depending on how fast
the knob is rotated.
TI-DS
Dia Pillar Illuminator 30W
Input ratings: 6 VDC, 30 W
Lamp ratings: 6 V 30 W halogen lamp
Specified lamp model: Halogen lamp (PHILIPS 5761)
Average lamp life: 100 hours
TE-PS30W
Power Supply A
TE-PSE30
Power Supply A
Input ratings: TE-PS30W: 100 to 120 VAC (10%), 50/60 Hz, 0.7 A
TE-PSE30: 230 VAC (10%), 50/60 Hz, 0.3 A
Built-in fuse ratings: 250 V F2AH
Output ratings: 6 VDC, 30 W
Maximum output current: 5.0 A
Electric shock protection class:
Class I
Remarks: TE-PS30W: UL listed product
TE-PSE30: GS approved
Temperature: 0 to +40C
Indoor use only
Transport and storage
conditions

127
Model name TI-ND6-PFS
Detectable object
Supported specimen: Cells in culture solution near the cover glass of a
glass-bottomed dish
Cover glass: Thickness: 150 to 180 m (No.1S)
Refractive index: 1.5
Culture solution Depth: 3 mm or greater
Refractive index: Approx. 1.33
Detectable boundary surface:
Water/oil immersion objectives: Boundary surface
between glass and specimen (culture solution)
Dry objectives: Boundary surface between glass and air
Recommended oil (for oil immersion):
Nikon Immersion Oil B (1250CST)
Detection method
Detection method: Active type, infrared LED light projection method
Detector: Inline CMOS sensor
Detector light source: Infrared LED
(wavelength: 880 nm, radiation power: 0.1 mW or less)
Offset control
Offset method Optical offset method
Controllable with the offset dial on the PFS offset controller
Offset Dry objectives: +100 to -10 m
Water immersion objectives: +20 to -2 m
Oil immersion objectives: 100x: +5 to -0.5 m
60x: +10 to -0.5 m
Note 1: Some objectives offer a wider offset range.
Note 2: The following objectives feature special offset ranges.
S Fluor DL 40xC: +30 to -10 m
Plan Apo 40x: +30 to -10 m
Plan Apo DM 40x: +30 to -10 m
Plan Apo TIRF 60xH: +5 to -0.5 m
Apo TIRF 60xH: +5 to -0.5 m
Focusing performance
Focusing time 700 msec or less at a position near the focal point
(at the boundary surface)
Focusing accuracy 1/3 or less of the focal depth of the objective
Applicable microscopy Bright-field, phase contrast, DIC, fluorescence, and TIRF
Built-in optical elements
IR filter: Passes light of 340 to 750 nm.
DM filter: Passes light of 340 to 770 nm and 925 to 1100 nm
Memory function
Offset registration/recall (for each objective, up to 6 total)
Nosepiece vertical position registration/recall (for each objective, up to 6 total)
Dimensions and mass
PFS motorized nosepiece:
Dimensions: 87 (H) x 232 (W) x 187 (D) mm
(excluding protrusions)
Mass: 2 kg
PFS offset controller: Dimensions: 99 (H) x 75 (W) x 72 (D) mm
(excluding protrusions)
Mass: 0.6 kg
Temperature: +20 to +38C
Controller only: 80% RH max. with no condensation
Indoor use only
Storage conditions
128
Device NA WD (mm) Type
Apo TIRF 60xH 1.49 0.12 Oil
Apo TIRF 100xH 1.49 0.12 Oil
Plan Apo 4x 0.2 15.7 Dry
Plan Apo 10x 0.45 4 Dry
Plan Apo 20x 0.75 1 Dry
Plan Apo 40x 0.95 0.14 Dry
Plan Apo DM 40x 0.95 0.14 Dry
Plan Apo 60xHA 1.4 0.21 Oil
Plan Apo 60xWI 1.2 0.22 WI
Plan Apo DM 60xHA 1.4 0.21 Oil
Plan Apo DM 60xH 1.4 0.21 Oil
Plan Apo 100xH 1.4 0.13 Oil
Plan Apo DM 100xH 1.4 0.13 Oil
Plan Apo TIRF 60xH 1.45 0.13 Oil
Plan Apo TIRF 100xH 1.45 0.13 Oil
Plan Apo VC 60xWI 1.2 0.27 WI
Plan Apo VC 60xH 1.4 0.13 Oil
Plan Apo VC 100xH 1.4 0.13 Oil
Plan Fluor 10x 0.3 16 Dry
Plan Fluor DLL 10x 0.3 16 Dry
Plan Fluor ELWD 20xC 0.45 7.4 Dry
Plan Fluor ELWD ADL 20xC 0.45 7.4 Dry
Plan Fluor ELWD DM 20xC 0.45 7.4 Dry
Plan Fluor 40x 0.75 0.72 Dry
Plan Fluor 40xH 1.3 0.2 Oil
Plan Fluor DLL 40x 0.75 0.72 Dry
Plan Fluor ELWD 40xC 0.6 3.7-2.7 Dry
Plan Fluor ELWD ADL 40xC 0.6 3.7-2.7 Dry
Plan Fluor ELWD DM 40xC 0.6 3.7-2.7 Dry
Plan Fluor 100xH 1.3 0.2 Oil
Plan Fluor ADH 100xH 1.3 0.19 Oil
Plan Fluor DLL 100xH 1.3 0.2 Oil
S Fluor 4x 0.2 15.5 Dry
S Fluor 10x 0.5 1.2 Dry
S Fluor 20x 0.75 1 Dry
S Fluor DL 20x 0.75 1 Dry
S Fluor 40xH 1.3 0.22 Oil
S Fluor DL 40x 0.9 0.3 Dry
Supported objective

7.4 AC Adapter
129
7.4 AC Adapter
Model name FSP120-ACB
Manufacturer FSP Group Inc.
Input voltage ratings 100 to 240 VAC, 2 A, 50-60 Hz
Voltage fluctuation 10%
Output voltage ratings 24 VDC
Output current ratings 5 A
Remarks UL listed, GS approved, CE compliant, and PSE approved product

7.5 Power Cord
7.5.1 Power Cord for the AC Adapter
AC inlet and plug
appearance

When used in 100-120 V
region, outside Japan
UL listed detachable power cord set, 3 conductor grounding
(3 conductor grounding Type SVT, No.18 AWG, 1.8 m long maximum,
rated at 125 VAC minimum)
region
Detachable power cord set approved according to EU/EN standard,
3 conductor grounding (3 conductor grounding Type H05VV-F, 2.5 m long maximum,
When used inside Japan
PSE approved detachable power cord set, 3 conductor grounding
(3 conductor grounding Type VCTF 3x0.75mm
2
, 1.8 m long maximum,
7.5.2 Power Cord for the Power Supply
AC inlet and plug
appearance

region, outside Japan
UL listed detachable power cord set, 3 conductor grounding
(3 conductor grounding Type SVT, No.18 AWG, 3 m long maximum,
region
Detachable power cord set approved according to EU/EN standard,
3 conductor grounding (3 conductor grounding Type H05VV-F, 3 m long maximum,
When used inside Japan
PSE approved detachable power cord set, 3 conductor grounding
(3 conductor grounding Type VCTF 3x0.75mm
2
, 3 m long maximum,

7.6 Safety Standards Compliance
130
7.6 Safety Standards Compliance
Configuration of
Ti-E microscope,
with TI-PS100W Power
Supply or TE-PS30W
Power Supply A
UL listed product.
This product meets FCC Part 15B Class A requirements:
This equipment has been tested and found to comply with the limits for a Class A
digital device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environment.
This equipment generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct the interference at his
own expense.
This product complies with Canadian EMI. (ICES-003 Class A)
Cet appreil numrique de classe A est conforme la norme NMB-003 du Canada.
Configuration of
Ti-E microscope,
with TI-PS100W Power
Supply or TE-PSE30
Power Supply A
CE marking
This product meets EU IVD Directive requirements.
(GM-approved: in vitro diagnostic medical device)
This product meets EU Low Voltage Directive requirements.
This product meets EU EMC Directive requirements. (EN61326)
This product complies with Australian EMC. (AS/NZS CISPR11)

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What safety precautions should be followed when using the microscope?

What are some of the objectives that can be used with the microscope?

Inverted Microscope

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