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    SRINIVASA RAO GANTA
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    This document contains 8 questions related to the topics of plant biotechnology and genetic engineering. The questions cover topics such as Ri and Ti plasmids, genetically engineered plants resistant to bacteria, production of secondary metabolites through plant tissue culture, development of haploid plants through anther culture, engineering of the provitamin A pathway in rice, plantibodies, cybrid development through protoplast fusion, and engineering plants for oxidative stress tolerance through superoxide dismutase genes. The document provides exam questions for a Plant Biotechnology course.

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    This document contains 8 questions related to the topics of plant biotechnology and genetic engineering. The questions cover topics such as Ri and Ti plasmids, genetically engineered plants resistant to bacteria, production of secondary metabolites through plant tissue culture, development of haploid plants through anther culture, engineering of the provitamin A pathway in rice, plantibodies, cybrid development through protoplast fusion, and engineering plants for oxidative stress tolerance through superoxide dismutase genes. The document provides exam questions for a Plant Biotechnology course.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    70 views4 pages

    Code No: 35060

    Uploaded by

    SRINIVASA RAO GANTA
    This document contains 8 questions related to the topics of plant biotechnology and genetic engineering. The questions cover topics such as Ri and Ti plasmids, genetically engineered plants resistant to bacteria, production of secondary metabolites through plant tissue culture, development of haploid plants through anther culture, engineering of the provitamin A pathway in rice, plantibodies, cybrid development through protoplast fusion, and engineering plants for oxidative stress tolerance through superoxide dismutase genes. The document provides exam questions for a Plant Biotechnology course.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
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    of 4

    Code No: 35060 R05 Set No - 1

    III B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009


    PLANT BIOTECHNOLOGY
    Bio-Technology
    Time: 3 hours Max Marks: 80
    Answer any FIVE Questions
    All Questions carry equal marks
    ?????

    1. Write about the following:

    (a) Ri Plasmid
    (b) Ti Plasmid. [8+8]

    2. Write about the genetically engineered plants for resistance against bacteria. [16]

    3. Do you think plant tissue culture can act as an alternative for the production of
    secondary metabolites. Explain. [16]

    4. Who first reported production of haploid plants through anther culture? What in
    your opinion are the most defining benefits of anther culture. [16]

    5. Discuss about the engineering of provitamin A (β-carotene) biosynthetic pathway


    into rice endosperm. [16]

    6. What are plantibodies. Describe with suitable example. [16]

    7. What are cybrids. How cybrids can be developed by protoplast fusion. [16]

    8. What is oxidative stress, Discuss how superoxide dismutase gene helps to produce
    transgenic plants resistant to oxidative stress. [16]

    ?????

    1
    Code No: 35060 R05 Set No - 2
    III B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009
    PLANT BIOTECHNOLOGY
    Bio-Technology
    Time: 3 hours Max Marks: 80
    Answer any FIVE Questions
    All Questions carry equal marks
    ?????

    1. Why do you think plants can be a suitable alternative to other living organisms for
    the production of biologically active compounds. [16]

    2. Write short notes on the following: [4 × 4]

    (a) Pharmaceuticals
    (b) Fragrances
    (c) Aroma
    (d) Food additives.

    3. Describe in detail Agrobacterium mediated gene transfer in plants. [16]

    4. Describe salt stress and discuss one approach for genetically engineering plants for
    salt stress. [16]

    5. What are the differences between organogenesis and somatic embryogenesis in vitro.
    [16]

    6. Suggest a strategy how plants can be protected against damage from several dif-
    ferent viruses. [16]

    7. Tissue culture can generate variations without mutagenic treatments justify this
    statement with suitable example. [16]

    8. Write about the reasons for the low production of secondary compounds while using
    in vitro cell culture system. [16]

    ?????

    2
    Code No: 35060 R05 Set No - 3
    III B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009
    PLANT BIOTECHNOLOGY
    Bio-Technology
    Time: 3 hours Max Marks: 80
    Answer any FIVE Questions
    All Questions carry equal marks
    ?????

    1. Give different examples of plant derived secondary metabolites of commercial value


    in pharmaceutical, food and cosmetics industries. [16]

    2. Discuss the detailed protocol of pollen culture. [16]

    3. Write short notes on the following: [4 × 4]

    (a) Salt stress


    (b) Oxidative stress
    (c) Chilling tolerance
    (d) Drought.

    4. What is metabolic engineering. Discuss about the strategy for the manipulation of
    secondary metabolic pathway. [16]

    5. Describe different pathways of morphogenesis in vitro. [16]

    6. Describe in detail about the production of cytokines and hormones in plants. [16]

    7. Discuss the status of transgenic crop plant production in India. [16]

    8. Describe how you would go about for the construction of plant transformation
    vectors and their use in gene transfer. [16]

    ?????

    3
    Code No: 35060 R05 Set No - 4
    III B.Tech I Semester Supplimentary Examinations,Nov/Dec 2009
    PLANT BIOTECHNOLOGY
    Bio-Technology
    Time: 3 hours Max Marks: 80
    Answer any FIVE Questions
    All Questions carry equal marks
    ?????

    1. How best you can keep your laminar flow contamination free, give specifications for
    sterilization of tissue culture media and thermo labile chemicals. [16]

    2. Write short notes on: [4 × 4]

    (a) Vir genes


    (b) Border sequences
    (c) Opines
    (d) T-DNA.

    3. How plants can be genetically engineered for salt tolerance. [16]

    4. Give an outline for the development of transgenic plants for biotic stress. [16]

    5. Discuss various pathways of haploid production emphasizing its merits and demer-
    its. [16]

    6. Write about the occurrence of xylanases in plants and its biotechnological applica-
    tions. Give an example of how xylanases can be produced in transgenic plants.
    [16]

    7. Give an account of the genetic engineering of plants for production of vitamin E.


    [16]

    8. What is the molecular basis of elicitation, discuss biotic and abiotic elicitors in
    detail. [16]

    ?????

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