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  • 511 Test and Exam Questions

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    KING DHEERA
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    This document outlines a test for the course BCH 511 - Cell and Tissue Culture Techniques. It contains 7 questions assessing key concepts in plant cell and tissue culture. Question 1 defines terms related to plant cell culture techniques. Question 2 describes distinguishing features of plant cells compared to other eukaryotic cells. Question 3 asks about important requirements for a cell culture laboratory. Question 4 explains the concept of plant tissue culture, types of culture media, technical issues that may arise, and components of media. Question 5 covers basic cell culture systems and steps in tissue culture. Question 6 details procedures for protoplast isolation and reasons for certain steps. Question 7 describes autoclave principles, operation, and applications.

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    Biochemistry

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    511 TEST AND EXAM QUESTIONS

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    This document outlines a test for the course BCH 511 - Cell and Tissue Culture Techniques. It contains 7 questions assessing key concepts in plant cell and tissue culture. Question 1 defines terms related to plant cell culture techniques. Question 2 describes distinguishing features of plant cells compared to other eukaryotic cells. Question 3 asks about important requirements for a cell culture laboratory. Question 4 explains the concept of plant tissue culture, types of culture media, technical issues that may arise, and components of media. Question 5 covers basic cell culture systems and steps in tissue culture. Question 6 details procedures for protoplast isolation and reasons for certain steps. Question 7 describes autoclave principles, operation, and applications.

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    23 views2 pages

    511 Test and Exam Questions

    Uploaded by

    KING DHEERA
    This document outlines a test for the course BCH 511 - Cell and Tissue Culture Techniques. It contains 7 questions assessing key concepts in plant cell and tissue culture. Question 1 defines terms related to plant cell culture techniques. Question 2 describes distinguishing features of plant cells compared to other eukaryotic cells. Question 3 asks about important requirements for a cell culture laboratory. Question 4 explains the concept of plant tissue culture, types of culture media, technical issues that may arise, and components of media. Question 5 covers basic cell culture systems and steps in tissue culture. Question 6 details procedures for protoplast isolation and reasons for certain steps. Question 7 describes autoclave principles, operation, and applications.

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    of 2

    FEDERAL UNIVERSITY OF TECHNOLOGY, OWERRI

    SCHOGL OF BIOLOGICAL SCIENCES


    DEPT OF BIOCHEMISTRY
    10/55/2020
    BCH 511 CELL AND TISSUE CULTURE TECHNIQUES
    TEST TIME: 30 MINS
    1. Define in one sentence the following terms: a.| Morphogenesis b.) b) Micropropagation
    c) Somaclonal variation
    d) Transgenic plants.
    e) Explant
    2. Describe the distinguishing features of plant tells from other types of eukaryotic o cells
    3. State the most important requirement of a cell culture laboratory
    Briefly explain the concept protoplast isolation and culture

    FEDERAL UNIVERSITY OF TECHNOLOGY, OWERRI


    SCHOOL OF BIOLOGICAL SCIENCES
    DEPARTMENT OF BIOCHEMISTRY
    2021/2022 RAIN SEMESTER EXAMINATION
    Course Code/Title: BCH 511 - Cell And Tissue Culture Technique (3 Units)
    Instructions: Answer Five (5) Questions Only.
    Time allowed: 3 Hours

    1a. What is Callus Culture?

    1b. Totipotency is an important characteristic of plant cells on which tissue culture techniques
    depend. Discuss.

    2a. Define the term "Sterilization"

    2b. Differentiate between dry heat and wet heat sterilization methods.

    2c. Enumerate the steps for complete explant sterilization.

    3a. Define the term "Transgenic plants"

    3b. Briefly describe the fundamental steps involved in production of transgenic plants

    3c. Give four (4) reasons for production of transgenic plants.


    4. Explain the concept of plant tissue culture stating its importance and highlight on the following
    (i) Three (3) types of culture media and their functions.
    (ii Three (3) technical issues that may arise during plant tissue culture technique.
    (iii) Three (3) major components and functions of a plant cell/tissue culture media.

    5aWhat are the two basic systems for growing cells in culture.

    5b.Describe the steps involved in tissue culture.

    6a.Using a descriptive diagram, highlight the procedural steps involved in protoplast isolation.

    6b) Give the reason why: (i). Plant epidermis is first treated with pectinase
    ii) Protoplast regeneration is regarded as protoplast development
    (iii) Liquid culture is preferred for protoplast cultivation
    (iv) Mechanical method of protoplast isolation is less utilized

    7 With annotated diagram describe the principle, operation and applications in the use of
    Autoclave

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