Chitosan Exerts Anticancer Activity Through Induction of Apoptosis and Cell Cycle Arrest in Oral Cancer Cells
Chitosan Exerts Anticancer Activity Through Induction of Apoptosis and Cell Cycle Arrest in Oral Cancer Cells
Chitosan Exerts Anticancer Activity Through Induction of Apoptosis and Cell Cycle Arrest in Oral Cancer Cells
Original
Introduction
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A B C
Fig. 1Morphology of apoptotic cells in the TUNEL assay. Arrows indicate cells showing
all the characteristics of apopotosis: (A) condensation of chromatin and cytoplasm, (B)
cytoplasmic fragments with or without condensed chromatin (apoptotic bodies), and (C)
chromatin fragments (micronuclei).
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Results
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Fig. 4Scanning electron micrograph of Ca9-22 cells cultured for 24 h in the presence of
LMWC (A) or cisplatin (B) in vitro (SEM, 200). Transmission electron micrograph of
Ca9-22 cells cultured for 24 h in the presence of LMWC, showing cell shrinkage, margination of nuclear chromatin and prominent mitochondria (C); or in the presence of cisplatin,
showing cell shrinkage, nuclear fragmentation, cytoplasmic vacuoles and prominent mitochondria (D) in vitro (TEM, 5,000).
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Discussion
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Acknowledgments
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JSPS-DGHE Joint Research Project of the Japan Society for the
Promotion of Science (JSPS). The authors are grateful to all the
staff at the Oral Biology Laboratory Universitas Indonesia and
Fellowship students at Tokyo Medical and Dental University,
Japan, for their kind help and support throughout this study.
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