Manual Usuario Humastar 600
Manual Usuario Humastar 600
Manual Usuario Humastar 600
| Service Manual
REVISION DESCRIPTION
01/2008-06
First edition
02/2012-09
03/2015-02
SYSTEM VERSION
COPYRIGHT
Copyright 2010, Human Gesellschaft fr Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of HUMAN in writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
CONTENTS
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS
1.1INTRODUCTION
2 SYSTEM DESCRIPTION
11
11
12
14
14
14
16
19
23
34
3 SECTION II
3.1 GENERAL TROUBLESHOOTING
39
39
39
40
40
44
46
46
47
47
47
48
49
50
51
51
51
52
52
53
53
53
54
54
55
4 TROUBLE SHOOTING
57
57
4.1.1Temperature
4.1.2 Stray light
4.1.3Noise
4.1.4Stability
4.1.5 Tip Pump
4.1.6 Level detection
4.1.7 Washer hydraulics
4.1.8Washer
4.1.9Dilution
4.1.10 Photometer linearity
4.1.11 Diluter linearity
4.1.12 Chemistry analysis
4.1.13 Clot detector
57
57
58
58
58
59
59
60
60
61
61
61
62
5 SECTION IV
133
6 APPENDIX
141
CONTENTS
Safety Instructions
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered part of the instrument and must be available to the
operator and the maintenance personnel. For accurate installation, use and
maintenance, please read the following instructions carefully.
In order to avoid damage to the instrument or personal injury, carefully read
the GENERAL SAFETY WARNINGS, describing the appropriate operating procedures. Please contact your HUMAN authorised local Technical Service in the
event of instrument failure or other difficulties with the instrument.
[IVD]
Safety Instructions
Safety Instructions
Notes:
10
System description
11
2 SYSTEM DESCRIPTION
Diluter
Probe assembly
Reaction tray
Dual beam photometer reading channel
Peristaltic pump for cuvette washer
Probe tip cleaning pump
Reaction aspiration pump
Drying pump
12
System description
The reading is obtained as the ratio of each signal to the reference signal, and
the system is therefore immune to source fluctuations, filter variations or dirt
accumulation on optical surfaces.
This double beam design allows the detection of reaction cuvettes in the reaction tray setting an alarm when cuvettes are missing or defective.
Automatic cuvette washers
There are two washing stations, one for each reaction tray. First pipe aspirates
test liquids and delivers washing solution; next three pipes deliver washing
solution and empty cuvettes; and the last one contains a drying block.
Operation of instrument is commanded by two intelligent microcomputers known as concentrators (C1 and C2) to handle communication packets
between computer and instrument devices providing the following services:
Information exchange to specific devices
Parameters storage
Packets tracking / detection of lost packets. Provision of a standard communication framework
Type
C1
C2
13
14
System description
15
Figure 3
Software configuration: From Maintenance > Parameters > Software > General
tab:
16
System description
1.
2.
3.
4.
17
18
SPECIFICATION
Voltage, Switching 24VDC MAX
Current, Switching 100 mA
Current, Carry 200 mA
Figure 7
System description
19
Hydraulic tab:
Select range and reaction tray to wash cuvettes.
Choose dry only when cuvettes are already clean to enhance drying.
Turn off washing pumps. To quiet pumps after a washing operation.
System flush. to purge both tubings and syringe.
Tip cleaning. To perform wash/soak of probe tip.
Intensive cuvette cleaning. Operator can select volume, time of action and
washing solution. This action is very useful for cuvettes used with latex type of
reagents or other contaminant fluids. Perform it at the end of the working day.
20
Photometer tab:
Press buttons to give full power or Stand by of the lamp Cuvettes.
To perform a report of absorbance cuvette
Figure 10
Motors tab:
Press turn off to de- enegize all motors.
System description
21
ISE tab:
Startup button performs all the operations required by the module (date, wet,
etc.), before starting to operate it.
Cleaning operation requires to have the ISE cleaning solution in the ray.
Use Empty/Fill chamber button to load/unload the ISE liquid column with the
ISE cup contents.
22
Purge button starts a process to remove the air and bubbles from the tubings.
Calibration button performs the internal two-point calibration.
Wet starts a sequence to maintain the membrane humidity within the appropiate levels. Dispense Std A/B activates the pinch valve to fill the the ISE cup.
Remember to press the fill chamber button to load the ISE liquid column.
Pinch valve time button sets the dispensed volume in the ISE cup. To note, when
dispensing three times Std A/B, volume must be 500uL in the ISE cup.
Electrode conditioning. Perform a conditioning with serum from prime position.
Figure 12
Wear tab:
Displays amount of use cycles, limits of use and last replacement date.
System description
23
24
Figure 13
Vertical tab:
Use selector to choose for front or back arms.
Initialize, moves the vertical to home position.
Move to position, locates the vertical on desired number of steps down home.
Find level, moves the probe down looking for liquid level, slow value, is the position to start looking for level while moving slower, bottom value represents the
maximum alowable steps to go down, and down level value is the amount of
steps to submerse the probe into the liquid.
Move up slowly button moves the boom up slowly and increases the speed after
the number of steps defined by the Slow steps parameter.
Turn on/off vibrator buttons. Mixer system control.
Turn off motor: Unblock motor.
System description
25
Horizontal tab:
Choose front or back arm and then initialize to move the horizontal to home
position, Move to position, to move the desired number of steps from home,
Move to sample position, to locate an specific sample number into the specific
sector. (Sector must be already located on the
tray).
Move to reagent, reaction, washing and ISE position, work similar to previous
buttons.:
Turn off motor: Unblock motor.
Figure 14
Trays tab:
Choose desired tray and use initialize to move tray to home, Move to tray position, to advance a
number of steps, and Move to sample position: for an specific sample number,
sector and arm.
Turn off motor: Unblock motor.
26
Figure 15
Pumps tab:
Select front or back, for peristaltic pump and then, initialize, advance or go back.
Front/back Tip pump when set to 1, will be on an amount of time given by the
timeout parameter. Note: set diluter to bypass position before activation of tip
pump.
Turn off motor: Unblock motor.
Figure 16
System description
27
Washing stations:
Choose for front or back wash head, then initialize, move to position, or move
to position using a second speed for avoid spillage during drying block cleaning.
Drying pump, when set to 1 turns on the drying block.
Water suction pump, when set to 1 turns on stations 2,3,4 and 5.
Reagent suction pump, when set to 1 turns on stations 1.
Turn off motor: Unblock motor.
Figure 17
28
Temperature tab:
Choose temperature controller then Initialize, to tranfer internal PID parameters, read temp, set temp and disable temp to operate temperature module.
Figure 18
Diluter tab:
Choose front or back, Initialize
And then Move to (steps, speed)
Set baud rate button. Refer to AR23 Diluter replacement procedure
Valve positions:
-- bypass is pump to tip
-- input is pump to syringe
-- output is syringe to probe
System description
29
Figure 19
BCR tab:
Press Initialize to start operation.
Read, shows result of barcode in front of BCR device.
Program, sets internal BCR
device parameters.
Advanced reading options:
Read sector code (tray position) Read sample code (sect, pos) Read reagent code
(position)
Figure 20
30
ISE tab:
Press Identify to check the ISE requests (date, calibration, etc.) Liquids button
informs the remaining volume of standards in the kit.
Use Empty/Fill chamber button to load/unload the ISE liquid column with
the ISE cup contents.
Purge button starts a process to remove the air and bubbles from the tubings.
Send date loads the current system date into the ISE module.
Urine/serum sample button sets the measurement mode.
Measure triggers the measurement process.
Wet button starts a sequence to maintain the membrane humidity within the
appropiate levels
Rinse button is used to perform a longer washing process than wet button.
Custom button sends ISE specific commands to the module.
Parameter section allows to inspect/modify parameters for the different
operating modes
Figure 21
System description
31
32
Figure 23
Concentrator tab:
Press AYA button to check the communication from PC to CPU.
Press Reset button to perform a reset of complete instrument.
Figure 24
System description
33
Stress tab:
It moves randomly desired number of cycles and show errors of each movement.
Check errorslog.txt to see errors details.
34
System description
621
Tx: 06000208007C006026
13:36:9.812
622
Rx: 05010208007C15BC
13:36:9.828
623
Tx: 06000209007D000C26
13:36:9.828
624
Rx: 05010209007D152C
13:36:9.828
625 Rx:
0E0002080078000000000000000000E7AB
13:36:10.125
626
Low level communication
627
Tx: 050102080078D6BD
(including ACK packets, packets
Rx: 0E000209007900000000000000000
numbers and CRC information).
0E107 13:36:10.125
13:36:10.125
628
Tx: 050102090079D62D
13:36:10.125
629 Rx:
0D0001100025000000000000006076A8
13:36:11.531
630
Tx: 0501011000256CFC
13:36:11.531
631 Rx:
0D000111002600000000000000607AB8
13:36:11.953
On next page, a table indicates how to translate high level communication to
obtain device specific details.
35
36
Table 1
System description
37
38
SECTION II
3 SECTION II
Corrective Action
Verify hydraulic system in accordance to users manual.
Clean probe tip by submerging in Solution 1 for 5 minutes.
Drops on tip after wash cycle.
Verify hydraulic system for leaks or
obstructions.
Abnormal noises.
Defective fans.
Moving parts blocked or frozen.
Temperature in reaction tray is too
Room temperature too high, (should
high. (Do not be concerned about arm always be at least 4C lower than seprobe temperature)
lected working temperature).
Example: For 37C incubation temperature, Room temperature should not
exceed 33C.
Temperature in reaction tray is too
Room temperature excessively low.
low. (Do not be concerned about
Verify instrument operating range,
arm probe temperature)
and adequate the room temperature.
39
40
High cross-contamination
Corrective Action
Verify that all pumps are working.
Verify that no tubing are clogged
Replace drying block
Calibrate washer unit position.
Identify cross-contaminants and set
methods in the Table of interferences
Increase the wash volume
Increase the number of wash cycles
SECTION II
41
42
SECTION II
43
Corrective Action
-----
44
Corrective Action
-- Dont change filling status of-- Bad calibration of scale thresholds in the
bottles.
controller board setting again.
-- Check base of bottles, it should be move
free and only touch load cell.
-- Connector of load cell is not connected
-- Load cell is connected in a wrong position.
-- Dont repeat threshold levels. -- Bad calibration of scale thresholds in the
controller board setting again.
-- Check base of bottles, it should be move
free and only touch load cell.
ION
Any
Any
Any
Any
Error in filling
Any
DESCRIPTIN
No startup
No startup
No startup
No startup
CORRECTIVE ACTION
Install a valid kit
Replace ISE pack
Replace ISE pack
Utilize ISE pack designed for your instrument and/or country.
Inspect pump tubing, remove from
No data
pump and rub with fingers. When reacquired or
peated, ISE module operation is abortcalibration
ed. Check for valves, inlet tubing and
performed
pressure in
pack bottles,
No access to the Inspect peristaltic pump and tubing.
following sample Check leaks and kinks in tubing
or no calibration and electrodes.
SECTION II
Na unstable
(*)
K unstable
(*)
Cl unstable
(*)
Timeout
45
Sodium
No stable
plateau reached
in samples and
calibration.
Slope out of the
allowed range
No stable
Electrode deteriorated. Erroneous
plateau reached threshold in module.
Potassium in samples and
Some samples can display this mescalibration.
sage. Only check if it persists for many
Slope out of the samples.
allowed range
No stable
plateau reached Electrode deteriorated. Erroneous
in samples
threshold in module.
Sodium
and caliSome samples can display this mesbration. Slope
sage. Only check if it persists for many
out of the
samples.
allowed range
Erroneous date orCommunication interrupted. Instru-----module missment off.
connection.
46
SECTION II
47
1. Turn on instrument.
2. Check if red power push button is blinking (No blink=all power supplies are
OK).
3. Check the green led located on each power supply.
4. In case led is off, test the power supply without load, follow schematic diagrams to find the short circuit.
5. Disable power on off board (see schematics).
48
SECTION II
49
50
SECTION II
51
52
Note:
When no faulty tubing is identified, use the tubing kit to externally bypass each
tubing and repeat the test to isolate the faulty one.
SECTION II
Perform Level detecion test (Maintenance > system tests > Level detection).
In case probe stops on air (falsely detecting liquid level),
Check lab neutral to ground mains below to 1V (both DC and AC modes).
Check or replace probe.
Check or replace level sensing board.
Check or replace wiring from level sensing board to distribution board.
53
54
SECTION II
55
Figure 29
56
MOVEMENTS
VERTICAL
HORIZONTAL
REACTION
SAMPLE
REAGENT
WASHER
PARAMETERS
Cat. N
Mass
With
Span
Freq. Range
Value
Cat. N
Mass
With
Span
Freq. Range
Value
Cat. N
Mass
With
Span
Freq. Range
Value
Cat. N
Mass
With
Span
Freq. Range
Value
Cat. N
Mass
With
Span
Freq. Range
Value
Cat. N
Mass
With
Span
Freq. Range
Value
SN: XXXX41XX
147 MXL
1,3
6
170
Standard
13 -17
2MR-186-06
1,3
6
50
Standard
25-40
3MR- 630-06
4,5
6
205
Standard
27-60
283XL
2,4
10
70
Standard
18-22
220XL
2,4
10
120
Standard
30-35
640 MXL
1,3
6
60
Standard
18-22
SN: XXXX40XX
136 MXL
1,3
6
150
Standard
13 -17
72MXL
1,3
6
50
Standard
25-40
280XL
2,4
6
180
Standard
27-60
283XL
2,4
10
70
Standard
18-22
220XL
2,4
10
120
Standard
27-60
640 MXL
1,3
6
60
Standard
18-22
Trouble Shooting
4 TROUBLE SHOOTING
4.1.1 TEMPERATURE
This test measures the time required for reaching preset temperatures and
final stability of reading. It includes cooler tray temperature. The test records
the minimum reached temperature and the last time in which the temperature reached the band between minimum and maximum allowed values. If the
temperature is turned off, the test stops and a warning is issued.
57
58
UV blocking, if visible is passed, indicates actual filter stray light. The use of Sodium Nitrite, 50 g/l and reading at 340 nm is recommended.
There are two options: either instrument dispenses solutions or user put them
directly in the selected cuvettes. Use Already dispensed for selection.
Test is passed if read values are less than 0.1%T.
Volume selection can be used to determine minimum volume that can be safely
measured.
4.1.3 NOISE
This test determines the departure of individual readings from the mean
value. Noise is evaluated separately from drift. For stability evaluation, total
time (Number of readings X Time interval) should be at least 10 minutes. Noise
evaluation is performed without moving tray and data are directly related to
photometer behavior.
When Absorbance correction is selected (recommended for solutions and not
for filters), results are expressed as equivalent to 1 cm cuvette measurements.
Noise test is relevant for absorbances over 1.300. Potassium Chromate (1.2 to
1.5 g/l in acidic media) is recommended.
Relevant data are peak-to-peak (maximum) difference. They should not exceed
0.002 for 1 minute total time.
4.1.4 STABILITY
Stability test is very similar to noise test, but the tray is randomly moving between readings. Comparison of data from noise and stability tests can give a
hint on mechanical positioning problems. Use conditions as described in 1.7.3.
Trouble Shooting
59
60
A liquid pumping level stability test will be performed dispensing water with D1
to D4, the number of times determined by the parameter, in different cuvettes
(positions 6 to 9, 10 to 13,) and measuring the liquid level with sensor probe. The
following volumes will be recorded for each station (1 to 4):
individual measurements;
average volume;
standard deviation and variance;
minimum and maximum volumes;
difference between minimum and maximum volumes;
difference between minimum and maximum average volumes of the
4 stations;
relative deviation error of average volumes.
A cuvette wash will be performed in all the used cuvettes.
4.1.8 WASHER
Washer test consists of performing cuvette cleaning cycle on a programmed
number of cuvettes. Absorbances are read on new cuvettes before cleaning action, immediately after cleaning and at some fixed time (Drying time). All three
data are shown in the graph.
If cuvettes are properly dried and not scratched by the system, values should
return to the original ones, with a tolerance of about 0.020 abs.
4.1.9 DILUTION
Dilution test should be performed with a sample of Potassium Chromate of 5 g/l
in acidic solution. Use as reagent the tip washing solution.
For a final volume of 4/400, CV should be less than 1.5%
Trouble Shooting
61
62
Trouble Shooting
63
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and item 3 can be
skipped. Once Start button is pressed, calibration can be aborted by pressing the
Skip button.
2. Arm and Reaction Tray
This calibration will define that tip falls in the reaction tray in the middle of the
reaction cuvette. Also, it defines the cuvette vertical position, which in turn, will
define the dispensing height. Calibration includes positioning of cuvette washer module.
1. Select Front Tray; remove cuvette cover and cuvette retainer cover.
2. Press F1 function or Start button.
3. Use buttons or letters A and D in keyboard until tip is close to the center of
cuvettes. Use 10-step or 1-step option as required.
4. Use buttons or letters W and S in keyboard until tip is few millimeters above
cuvette.
5. Rotate tray by using buttons or letters Q and E in keyboard until cuvette
number 1 (labeled with a sticker) coincides with tip position.
6. Repeat steps 3 and 5 until tip falls in the middle of cuvette number 1. For
better sensitivity, use 1-step buttons. Do not fine tune vertical position at
this time.
7. Press F3 function or Confirm button
8. Press F3 function or Confirm button.
9. Shift probe horizontally until tip is above cuvette body but outside cuvette
itself.
10. Use buttons or letters W and S in keyboard and 1-step mode until tip just
touches upper flat part of cuvette body.
11. Press F3 function or Confirm button.
12. Select Back Tray and repeat procedure.
Figure 32
64
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and item 3 can be
skipped.
Once Start button is pressed, partial calibrations can be aborted by pressing the
Skip button.
Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.
3. Washer
Use this window to calibrate the position when the drying block goes down to a
cuvette. From the washer calibration window
1. Choose front or back washers, and then push Start.
2. Loosen washer head screws.
Figure 33
3. Use buttons or letters R and F in keyboard to download the washer into the
cuvette. When moving down the washer, use the index finger to move up
the dryer block, release it, and check if moves down freely. Center washer
pipes in the cuvette. Pre adjust screws.
Trouble Shooting
65
Figure 34
Figure 35
4. Continue moving down until the dryer block reaches the cuvette bottom.
The dryer spring should not be activated. Stop before it happens.
Figure 36
66
5. Confirm than the dryer block moves freely, and the pipes are centered in the
cuvette and tighten screws.
Figure 37
Figure 38
6. Continue moving down until station 5 spring compresses about 0.5 mm. The
block dryer block should not move the reaction tray, you have to test it with
test button. Use 10-step or 1-step option as required.
Figure 39
Trouble Shooting
67
68
7. Use buttons or letters W and S until tip just touches bottom of sample vial.
Pull up frequently the vial while stepping down
8. Press F3 function or Confirm button.
9. Use buttons or letters A and D in keyboard until tip is close to the center of
outer sample ring. Use 10-step or 1-step option as required.
10. Rotate Sample tray by using buttons or keys Q and E in keyboard.
11. Repeat 3 and 4 until tip is in the center of sample vial number 2.
12. Press F3 function or Confirm button.
13. Use buttons or letters W and S until tip just touches bottom of sample vial.
Pull up frequently the vial while stepping down
14. Press F3 function or Confirm button.
15. Select Back Probe and repeat procedure.
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and items 3, 4, 9,
and 10 can be skipped. Once Start button is pressed, partial calibrations can be
aborted by pressing the Skip button.
Important: Before to confirm a position, press F5 to test this movement in
normal operation to avoid a bad calibration due to backlash.
6. Arm and Reagent Tray
1.
2.
3.
4.
5.
6.
7.
8.
9.
Trouble Shooting
69
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and items 4 and 9 can
be skipped. Once Start button is pressed, partial calibrations can be aborted by
pressing the Skip button.
Important: Before to confirm a position, press F5 to test this movement in
normal operation to avoid a bad calibration due to backlash.
70
7. Sample Tray
This calibration is intended for alignment of sample sectors into the removal
area.
1. Press F1 function or Start button.
2. Rotate Sample tray by using buttons or keys Q and E in keyboard until zone
1 is visible in the load area.
3. Re-adjust until sector can be loaded and unloaded through the loading area.
4. Press F3 function or Confirm button.
Figure 42
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and item 1 can be
skipped.
Once Start button is pressed, calibration can be aborted by pressing the Skip
button.
Important: Before to confirm a position, press F5 to test this movement in
normal operation to avoid a bad calibration due to backlash.
Trouble Shooting
71
8. Reagent Tray
This calibration is intended for alignment of reagents into the removal area.
1. Press F1 function or Start button.
2. Rotate Reagent tray by using buttons or keys Q and E in keyboard until Reagents 1 and 25 are visible in the load area.
3. Re-adjust until reagents 1 and 25 can be loaded and unloaded through the
loading area.
4. Press F3 function or Confirm button.
Figure 43
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and item 1 can be
skipped. Once Start button is pressed, calibration can be aborted by pressing the
Skip button.
9. Bar Code Reader
1. Press F1 function or Start button.
2. Install in reagent 1 position a vial with valid bar code.
3. Use buttons or letters A and D in keyboard until vial is in front of BCR window. Use the 1-step option.
4. Press button or key R in keyboard and verify if code is read.
5. Repeat steps 3 and 4 until code is read. Look for the central position if code is
read in a range of positions.
72
If instrument was already calibrated, Last button will position tray where last
calibration was determined. Once Start button is pressed, partial calibrations
can be aborted by pressing the Skip button.
10. ISE Module
1. Press F1 function or Start button.
2. Use buttons or letters A and D in keyboard until tip is close to the center of
ISE loading window. Use 10-step or 1-step option as required.
3. Press F3 function or Confirm button.
4. Use buttons or letters S and W in keyboard until tip touch bottom cup. Use
10-step or 1-step option as required.
5. Press F3 function or Confirm button.
6. Use buttons or letters A and D in keyboard until tip is close to the center of
ISE priming position. Use 10-step or 1-step option as required.
7. Press F3 function or Confirm button.
8. Use buttons or letters S and W in keyboard until tip is in the bottom of the ISE
priming position. Use 10-step or 1-step option as required.
9. Press F3 function or Confirm button.
Trouble Shooting
73
If instrument was already calibrated, Last button will position tray where last
calibration was determined. This procedure will save time and item 2 can be
skipped.
Once Start button is pressed, partial calibrations can be aborted by pressing the
Skip button.
Important: Before to confirm a position, press F5 to test this movement in normal operation to avoid a bad calibration due to backlash.
74
Figure 45
Trouble Shooting
75
76
Trouble Shooting
77
Figure 52
1 Wash head screws
2 Collision sensitivity
1
2
78
xv. AR-15: Reagent tray belt replacement and tension belt adjustment
*Belt replacement
1. Perform AR-25: Removal of complete reagent cooler assembly procedure.
Steps from 1 to 21.
2. Remove reagent verification sensor and motor.
3. Remove belt.
*Tension belt adjustment
1. Release screws. See picture.
2. Move motor to adjust the tension belt.
3. Perform VD-19 to verify tension and values settings.
Figure 54
Trouble Shooting
79
xvi. AR-16: Vertical arm belt replacement and tension belt adjustment
*Belt replacement
1. Perform AR-09: Removal of complete boom assembly (vertical & horizontal)
2. Release screw from pulley support. See picture
3. Move pulley support and remove the vertical verification sensor and release
the guiding shaft to loose and remove the belt.
*Tension belt adjustment
1. Perform AR-09: Removal of complete boom assembly (vertical & horizontal)
2. Identify the vertical belt.
3. Move pulley support to adjust belt tension.
4. Perform VD-19 to verify tension and values settings.
Figure 55
80
Trouble Shooting
81
Figure 57
82
Figure 59
Trouble Shooting
83
84
3.
----
In case readings of front, back and ref channels are not similar, perform AR-19:
Adjustment of filter delays. In case issue persists, perform VD-06: Photometer
and lamp alignment check.
Trouble Shooting
85
86
Trouble Shooting
87
7. Remove the supply connection and the data connection of the fridge controller (see three arrows).
Figure 68
8. Removing the drain condensation tubing from the fridge. See hydraulic
diagram.
Figure 69
88
9. Identify the fridge assembly inside the instrument. On the right side of the
picture, you will see two post to support guiding wheels for the sample tray
metal ring.
Figure 70
10. Identify the first post located on the right of the fridge assembly, perform
a marking using a permanent marker. The mark should be a straight line
joining both the positioning rod and the flat surface as shown in the picture.
This is to be able to locate this part exactly to the same position when re(
assembly.
Figure 71
Trouble Shooting
89
12. Identify the hole and use an allen wrench to rotate the post about 90o, this
will turn an eccentric piece that will release the tray.
Figure 73
13. Release the pulley using an hex wrench and a 10mm wrench from the other
side.
Figure 74
14. Remove the belt by gently sliding out of the big pulley of the sample tray.
Figure 75
90
15. Remove the sample ring by slowly and gently rotating the sample ring counter- clockwise while lifting it.
Figure 76
17. Use a thin pin and a hammer to hit and remove the locking pin of the white
plastic support.
Figure 78
Trouble Shooting
91
20. Identify the four screws you will need to remove to separate the fridge from
its support.
Figure 81
92
21. Identify the four screws you will need to remove to separate the fridge from
its support.
Figure 82
22. Identify the four screws you will need to remove to separate the fridge from
its support.
Figure 83
23. Identify the four screws you will need to remove to separate the fridge from
its support.
Figure 84
Trouble Shooting
93
25. Gently pull the fridge (the white part that has attached two square pipes
with fans). Before pulling from the fridge, it is necessary to release the condensation tubing (shown in previous step) and also check if it is necessary to
disconnect any other wire from a sensor before pulling the fridge.
Figure 86
26. Use a soft cloth on the table to protect against scratchings the delicate surfaceof the fridge. Put the fridge upside down.
Figure 87
94
27. Disconnect the sensor (see flat wire is disconnected from the board).
Figure 88
28. There are three screws holding each square pipe. Slide the fridge towards
you to be able to access the screws (the screws are located inside of chamber
where is normally located the reagent tray) and remove the three screws.
Then lift the square pipe that has attached the controller board to access
peltier elements as shown.
Notice that peltier elements are mounted in this fashion:
Graphite sheet + Peltier element + Graphite sheet
The graphite sheet improves thermal conductivity between peltiers and
fridge surfaces. Take care of the graphite sheets, because are very delicate
and easy to break.
In case you break a graphite sheet replace it and clean properly with alcohol the
surface of the peltier elements to avoid any residues on them.
Figure 89
Trouble Shooting
95
29. During the release of the screws attached to the pipe, take care of the
support of each screw.
Figure 90
31. Identify the new sensor, it should have two screws and two white washers.
Figure 92
96
Trouble Shooting
97
35. Screw the sensor do not force the screws, just screw to ensure sensor is in
proper contact with the metal surface.
Figure 96
98
38. Final view of the sensor mounted with the silicone sealant.
Figure 99
39. Identify the screws supports and position them properly on the fridge
Figure 100
Trouble Shooting
99
WARNING!!: When reassembling the fridge back to the fridge support, take care
that shaft should be properly centered on its hole before tightening the screws
(See pictures below)
Figure 101
Figure 102
xxvi. AR-26: Adjust and verify probe optical collision sensor. M400-P317 Head
board.
1. Tip collision is detected by the interruption of the light emitted by an optical
sensor generated by the movement in the collision flag. When the flag is
moved, the hole in the flag will not be aligned anymore with the optical light
path of the sensor and the collision will be detected.
Figure 103
Figure 104
100
Figure 105
2. The collision flag hole must be aligned with the sensor optical path. You
must center vertical and horizontal sides of the collision flag with the optical
sensor. Collision flag must be equally distant from the sensor sides.
Figure 106
Figure 107
Figure 108
3. To achieve alignment, you will need to loosen board screws and move the
board up and down. Small adjustments can be obtained. Once finished, tighten the screws.
Figure 109
Trouble Shooting
101
4. If you cant perform the alignment procedure, you will need to use flat nose
pliers in order to adjust the collision flag.
Figure 110
Figure 111
5. To verify a correct adjustment go to Maintenance > service > Manual > Vertical Turn on and off vibrator quickly and check that the collision led (DL4) is
always turned off.
xxvii.
Lubrication procedure should be repeated every six months. For units with
continuous work that exceeds the 1500 analysis/day the procedure should be
repeated every four months.
Starting software version 1.8.1, an automatic warning will be issued when the
lubrication becomes necessary.
Use mineral oil SAE 15 or SAE 20. DO NOT USE MORE THAN TWO DROPS IN
EACH OILING POINT, otherwise excess of oil could fall on sensors and belts and
damage the system.
Oil can be added with an hypodermic syringe. For reference, two drops is
equivalent to 50 microliters.
102
Figure 112
Trouble Shooting
103
104
Trouble Shooting
105
Figure 118
106
Figure 120
Trouble Shooting
107
xxix. AR-29: Reaction tray belt replacement and tension belt adjustment
*Belt replacement
1. Release screws. See picture.
2. Move motor and remove the belt.
*Tension belt adjustment
1. Release screws. See picture.
2. Move motor to adjust the tension belt
3. Perform VD-19 to verify tension and values settings.
Figure 121
108
Figure 123
Trouble Shooting
109
110
8. Install the syringe and pull the syringe plunger until it is aligned with the
carriage. Align the valve using the plunger as a guide, and tighten from 1/4
to 1/2 turn after the screws contact the valve body.
9. Pull the syringe plunger all the way into the carriage and secure by tightening the plunger lock screw.
Figure 125
1.
2.
3.
4.
5.
6.
Trouble Shooting
111
Figure 126
112
3. Remove the halogen lamp from the socket pushing the lever.
Figure 129
Trouble Shooting
113
4. Place the new halogen lamp into the socket. Take care of the lamp polarity.
The big terminal should goes up.
Figure 130
114
2. Pull tube out of its lodging rotating by hand the pump rotor if necessary.
Figure 135
Trouble Shooting
115
116
Trouble Shooting
117
Figure 140
Figure 141
118
Figure 144
Trouble Shooting
119
4. For the 2 pumps in the left of the photo (FDP and FWP), in order to have
access to the pump head, you will need to remove the pump from the mounting base. Using a screwdriver remove the 3 mounting screws.
Figure 147
Figure 148
120
Figure 149
Figure 150
Trouble Shooting
121
Figure 154
Figure 155
122
3. Identify the pump which the filter will be replaced/cleaned. Due to biological
risk, we recommend to replace the FRP and BRP mesh filters. FWP and BWP
filters can be cleaned.
Figure 158
Figure 159
Trouble Shooting
123
Figure 160
Figure 161
124
2. Remove the equipment back, front and side panels in order to have access to
the washers and tip water pumps
Figure 163
3. You will need to replace the tubing from the washer, including the water
suction pump tubing (pipes 16 to 19), water delivery pump tubing (pipes 11
to 14), reaction pump tubing (pipe 15), and drying block pump tubing (pipe
28). Also you will need to replace the DI water intake tubing from the DI water bottle (T022F and T022B).
Figure 164
Trouble Shooting
125
Figure 165
126
2. Remove, clean, and place it again one at a time. Place the filters under the
tap in order to clean it. Dried properly before placing.
xlii. AR-42: Procedure for install instrument cover
1. Remove right lateral and rear panel.
2. Install the instrument cover in the position and place the hinge axis, do not
adjust the set screw in this step.
Figure 167
4. Check the adjustment of the instrument cover over the deck. If there isnt
collisions adjust the set screw.
Trouble Shooting
127
5. If you detect side collisions, between the instrument cover and decks borders turn the tensor screw as the schema to move the instrument cover to
the right position.
Figure 169
Figure 170
Figure 171
Figure 172
6. Adjust the rear hinges set screws, put the lock cup to the gas spring, and
place the lateral and rear panel.
128
Code
VOSB1206
M400BH21/3
M400BH19W
KITOIL4000
Description
Syringe Cavro 73804 for HS600
Peristaltic pump tubing
Washer filter
Lubrication Kit
Code
VOSB1206
M400BH21/3
M400BH19W
KITOIL4000
LAR12V20W
OTDI700A
OTDI700L
M40J14W
M400BH09W
M4000TUH
Description
Syringe Cavro 73804 for HS600
Peristaltic pump tubing
Washer filter
Lubrication Kit
Halogen Lamp 12V
Diaphragm for Drying pump
Diaphragm for Water pump
Drying block
Peristaltic pump rotor
Tubing Sets 12 month
Trouble Shooting
129
130
Table 2
TYPICAL
NAME
BLOCK
Cuvette Blank
Cuvette Blank
0,2
Tolerance
Cuvette Blank
0,04
Sample vials
950
Filter 2 (Lambda)
Filter 3 (Lambda)
Filter 4 (Lambda)
Filter 5 (Lambda)
Filter 6 (Lambda)
Filter 7 (Lambda)
Filter 8 (Lambda)
Filter 9 (Lambda)
Sample vials
950
Photometer
340
Photometer
Photometer
Photometer
Photometer
Photometer
Photometer
Photometer
Photometer
VALUE
380
405
450
490
505
550
590
620
650
700
COMMENTS
Minimum absorbance for
a valid cuvette
Maximum absorbance for
a valid cuvette
Tolerance to detect a dirty
cuvette after washing
Area for the default vial
Area for the secondary or
pediaric vial
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
Wavelenght for the specified filter
ACCESS
LEVEL
User
User
User
User
User
Service
PATH
Maintenance\Parameters\Use
Maintenance\Parameters\Use
Maintenance\Parameters\Use
Maintenance\Parameters\Use
Maintenance\Parameters\Use
Maintenance\Service\Parameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Trouble Shooting
Front Arm
Temperatures
131
750
41
Preheater temperature
Maintenance\Service\PaService
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Filters
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Back Arm
Reaction Tray
Tolerance (Abs)
Serum
Temperatures
Temperatures
Temperatures
Cuvette Blank
Cuvette Blank
Cuvette Blank
ISE
ISE Thresholds
41
39
8 (H) / 7 (L)
0,2
0,04
500
1700
Preheater temperature
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Urine
ISE Thresholds
3000
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Std A
ISE Thresholds
1500
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Std B
ISE Thresholds
Pumps
2500
750
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
132
Decompression
(ms)
Pump turns
Syringe cycles
Pumps
Wear
Wear
ISE number of
samples
Front volume
(Steps)
Back volume
(Steps)
Front time down
(ms)
Back time down
(ms)
Volume
Diluter speed
Wear
Washing Station
Washing Station
4750
250
50000
100000
20000
10000
1500
1500
Maintenance\Service\PaService
Others
Maintenance\Service\PaService
1300
Service
1300
Service
100
4320
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
drying cycle
Pre & Post Wash
rameters\Instrumental\
Others
Maintenance\Service\Pa-
drying cycle
Time to keep the wash
Washing Station
rameters\Instrumental\
Others
Maintenance\Service\Pa-
rameters\Instrumental\
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
Maintenance\Service\Pa-
Service
rameters\Instrumental\
Others
SECTION IV
133
5 SECTION IV
Charts and diagrams
a. Location of belts and sensors
Figure 173
Photometer assembly top view
Figure 174
Robot assembly overview
side view
134
Figure 175
Washer assembly side view
Figure 176
Sample and reagent tray
assembly bottom view
SECTION IV
135
Figure 177
Reaction tray bottom view
136
SECTION IV
137
c. Complementary information
i. Identification of system leds
Location
On each power supply
Led silkscreen/color
Green led
Description
Power good signal from power
supply
Power on push button
Red lamp
Blinks on a power supply fault
event
Backplane board
+36_1/Red
36v on J21*
PL400245
+36_2/Red
36v on J20*
+36_3/Red
36v on J60*
+36_4/Red
36v on J57*
+12/Green
12v on J72*
Bar code reader interfase
DL1/Green
Read Ok
board PL230224
DL2/Red
Read order / init to BCR
RS232 to RS232
+5vPC/Red
Power from standby power suppIsolated board
+5vCPU/Red
ly (allways on)
PL400282
Power from CPU board (on when
instrument is on)
Microconverter baseplane DL1/Green
+12 input
& Power supply board
DL2/Red
+12A output
PL400248
DL3/Red
-12A output
Power ON/OFF control board+12v cooler led/Red
Power good from power supply
PL400255
+12v lamp led/Red
Power good from power supply
+36v heater led/Red
Power good from power supply
+36v Motor II led/Red
Power good from power supply
+36v Motor I led/Red
Power good from power supply
+12v Logic led/Red
Power good from power supply
+24v Diluters led/Red Cooler
Power good from power supply
activated led Solid state relay/ JP1: cooler pushbutton bypass
Red
JP2: mains pushbutton bypass
Mains activated led Solid state
relay/Red
ISE protocol interfase board
+12
PL400268
DL1/Red
Ise busy
DL2/Red
RS485 Cavro Blink
DL3/Red
(development purposes)
DL4/Red
Trigger 1 (development purposes)
Cooler control board
DL1/Red
Power on J12/J13: Peltiers
PL400269
DL2/Red
Power on J14/J15: Peltiers
DL3/Green
+12
Table 3
138
DL1/Red
Lamp on signal
DL1/Red
DL2/Red
DL3/Red
DL4/Red
DL5/Red
DL6/Red
DL7/Red
DL8/Red
Notes:
* Remove all motor controller boards to perform led checkings (any motor controller internally
wires J21 to J20 and J60 to J57 depending its position on backplane).
-- Dual Stepper motor controller PL400242 status leds:
Normal Mode
Green led
Red 1 led
Red 2 led
-- On
-- Home signal
-- Verification signal
-- Collision(wa sher only)
Red 1 led
-- Home signal
-- On
-- Off
-- On
Red 2 led
-- Filter signal
-- On
-- Off
-- Off
To obtain the program folder press the right button on the autoanalyzer icon and choose
properties.
SECTION IV
139
Historic
Interfase
Lims
NSI data
Priv
Priv thread
Reports
Translator
Use data
iii.
MESSAGE
On line
Operating
Connecting
Offline
System log
OPERATION
Instrument is ready to process commands.
Instrument is busy.
Computer trying to set the link to the autoanalyzer.
Computer was not able to connect to the instrument.
Instrument was unable to recover from an error A fatal error occurred.
System Alerts
User attention required.
Reaction tray Full
Washer is disabled and no empty cuvettes available.
Reaction pending Acc.
Awaiting confirmation of result by the user.
Calculated pending Acc.
Awaiting confirmation of calculated result by the user.
Reagent blank pending Acc. Awaiting confirmation of measured blank by the user.
PreAutomatic
Instrument performing hydraulic priming and
reaction tray heater warm up.
Instrument processing samples (cuvette checking, reAutomatic
agent level checking, processing of blanks, standards,
controls and samples).
Post-Automatic
Instrument washing used cuvettes and cleaning the
probes with wash and soak solutions
140
APPENDIX
6 APPENDIX
a. Glossary
Absorbance. Absorbance, or optical density, is a measure of the amount of
light absorbed by a solution. Absorbance is equal to the logarithm of the ratio of
incident light to transmitted light.
Acceptance. The action of approving or rejecting the result of an assay. Used by
the operator to confirm calibrations or out of range assays results.
Accuracy. The closeness of the result of a measurement to the true value.
Aliquot. A measured portion of a sample taken for analysis.
Analyte. A specific compound or element of interest undergoing chemical
analysis.
Batch. A quantity of material produced or processed in one operation, considered to be a uniform discrete unit.
Batch-sample. One of the samples drawn from a batch.
Batch-size. The number of samples in a batch-lot.
Bichromatic measurement. The substraction of a secondary wavelenght
absorbance reading from a primary wavelenght absorbance reading to obtain a
delta absorbance reading.
BRTW. Back reaction tray and washer.
BVH. Back vertical & horizontal.
Calibrate. To determine, by measurement or comparison with a standard, the
correct value of each scale reading on a meter or other device, or the correct
value for each setting of a control knob.
Calibration curve. The graphical relationship between the known values for a
series of calibration standards and instrument responses.
Calibration drift. the difference between the instrument response and a
reference value after a period of time without recalibration.
Calibration standard. A substance or reference material used to calibrate an
instrument.
Certified Reference Material. A material that has been certified for accuracy,
stability and physical form.
Coefficient of variation (CV). A measure of relative dispersion (see precision). It
is equal to the ratio of the standard deviation divided by the arithmetic mean.
141
142
APPENDIX
143
144
Quality Control (QC). The operational techniques and activities that are used to
fulfil requirements for quality.
Reagent blank Reagent(s), without analyte or sample added, which are analyzed
to determine their contribution to the total absorbance reading.
Reliability. The likelihood that an instrument or device will function under
defined conditions for a specified period of time.
SRT. Sample & Reagent Tray.
Standard solution. A solution containing a known concentration of analytes,
prepared and verified by a prescribed method or procedure and used routinely
in an analytical method.
Standard. Standard samples are aliquots of calibration specimens containing
predetermined quantities of the analyte. The response of each standard, along
with the standards predetermined concentration, is used to construct a standard calibration curve. From this standard curve, sample concentrations can be
computed using the response from the sample.
Stat. Assay requiring immediate ressults.
TC. Temperature controller.
Test result. A product obtained from performing a test or assay determination.
UV. Ultraviolet.
APPENDIX
b. Technical specifications
Throughput:
450 tests/hour double reagent, 650 tests/tour
mono reagent, max. 770 tests/hour with optional ISE unit.
Reagents:
Maximum number of simultaneous tests: 36 double to
72 single reagent tests + 3 with optional ISE unit.
1 to 3 reagents, 5 to 500 L/test each (in
increments of 1 L), final total solution volume 180 to
Analysis Modes:
500
L/test
End point with sample or reagent blank. Factor
Reagent
bottles capacities: 25, 40 and 70. Reagent cooor standard.
ling
compartment:
72 cooled positions.
Priority selection by sample (profile) or by reagent (batch).
Reaction:
Calibration curve with up to 10 standards.
Water consumption: 3 L / hour.
Automatic curve fit. Turbidimetry.
Warm air incubator: room, 30C and 37C. Reaction
Fast and two-point kinetics (zero and first
cuvette: re-usable plastic 6 mm path light cuvettes
order).
Reaction time: 0 to 10 min.
Routine, batch, STAT procedures, profiles. En- Reaction temperature: 37C 0.1C. Stirring: After diszymes. Drugs.
pensing each reagent.
Automatic sample dilution on abnormal levels, Optic System: Double beam
excessive substrate consumption
Photometric Range: -0.1 to 3.6 A. Measuring waveand/ or lack of linearity.
length: 340 to 800 nm (selectable among 12 waveFull quality control: Levy-Jennings plots, West- lengths).
gard multirules.
Photometry: Single or Double-wavelength
Import/export data, methods and historic files. simultaneous reading.
Automatic backup procedure.
ISE Unit:
Test selection, automatic calibration, calibration Na+, K+ and Cl- measurements.
curve, multipoint calibration, polygonal.
Samples: serum or urine. Other electrodes on request.
Serum indices: sample blank compensation,
Data Management:
calculated tests, Quality control, auto re-run,
Windows TM based Software. Interface LIS: bi-directioprozone check, record of calibration, data stonal RC 232 C, according to ASTM 1394 requirements.
rage (historic results).
Printout: Customers optimized (Analysis result, work
Automatic pre-dilution and post-dilution
list,
serums list, Quality Control, Calibration curves, etc.)
(ratio 1:5 to 1:100)
Stat: Highest priority in operation. Continuous Power Requirements:
110/220V, 50/60 Hz, 2.0 kVA
sample load
Environmental Requirements
Sampling and reagent Samples:
Sample volume: 2 to 100 L/test (in increments Temperature: max 30 degree Celcius
of 0.2 L.
Humidity: max 80%
Sample Tray: 95 (5 racks x 19 positions) ID
Dimensions:
bar code equipped positions for routine, stat
97(w) x 67(D) x 100 (H) cm.
and control samples and standard solutions.
Primary tube (length up to 100 mm), Pediatric Weight:
115 Kg.
vial
145
146
HUMAN
Gesellschaft fr Biochemica und Diagnostica mbH
Max-Planck-Ring 21 65205 Wiesbaden Germany
Tel.: +49 6122/9988 0 Fax: +49 6122/9988 100
eMail: human@human.de www.human.de