13D Flow Injection Analysis

The focus of this chapter is on methods in which we measure a time-dependent signal. Chemical kinetic
methods and radiochemical methods are two examples. In this section we consider the technique of
flow injection analysis in which we inject the sample into a flowing carrier stream that gives rise to a
transient signal at the detector. Because the shape of this transient signal depends on the physical and
chemical kinetic processes occurring in the carrier stream during the time between injection and
detection, we include flow injection analysis in this chapter.
13D.1 Theory and Practice
Flow injection analysis (FIA) was developed in the mid-1970s as a highly efficient technique for the
automated analyses of samples.17 Unlike the centrifugal analyzer described earlier in this chapter, in
which the number of samples is limited by the transfer disks size, FIA allows for the rapid, sequential
analysis of an unlimited number of samples. FIA is one example of a continuous-flow analyzer, in which
we sequentially introduce samples at regular intervals into a liquid carrier stream that transports them
to the detector.
A schematic diagram detailing the basic components of a flow injection analyzer is shown in Figure
13.18. The reagent serving as the carrier is stored in a reservoir, and a propelling unit maintains a
constant flow of the carrier through a system of tubing that comprises the transport system. We inject
the sample directly into the flowing carrier stream, where it travels through one or more mixing and
reaction zones before reaching the detectors flow-cell. Figure 13.18 is the simplest design for a flow
injection analyzer, consisting of a single channel and one reagent reservoir. Multiple channel
instruments that merge together separate channels, each introducing a new reagent into the carrier
stream, also are possible. A more detailed discussion of FIA instrumentation is found in the next section.

Figure 13.18 Schematic diagram of a simple flow injection analyzer showing its basic components. After
its injection into the carrier stream the samples mixes and reacts with the carrier streams reagents
before reaching the detector.
When we first inject a sample into the carrier stream it has the rectangular flow profile of width w as
shown in Figure 13.19a. As the sample moves through the mixing and reaction zone, the width of its
flow profile increases as the sample disperses into the carrier stream. Dispersion results from two
processes: convection due to the flow of the carrier stream and diffusion due to the concentration
gradient between the sample and the carrier stream. Convection occurs by laminar flow. The linear

velocity of the sample at the tubes walls is zero, but the sample at the center of the tube moves with a
linear velocity twice that of the carrier stream. The result is the parabolic flow profile shown in Figure
13.19b. Convection is the primary means of dispersion in the first 100 ms following the samples
injection.

Figure 13.19 Effect of dispersion on the shape of a samples flow profile at different times during a flow
injection analysis: (a) at injection; (b) when convection dominates dispersion; (c) when convection and
diffusion contribute to dispersion; and (d) when diffusion dominates dispersion. The red line shows the
width, w, of the samples flow profile.
The second contribution to the samples dispersion is diffusion due to the concentration gradient
between the sample and the carrier stream. As shown in Figure 13.20, diffusion occurs parallel (axially)
and perpendicular (radially) to the direction in which the carrier stream is moving. Only radial diffusion is
important in flow injection analysis. Radial diffusion decreases the samples linear velocity at the center
of the tubing, while the sample at the edge of the tubing experiences an increase in its linear velocity.
Diffusion helps to maintain the integrity of the samples flow profile (Figure 13.19c), preventing samples
in the carrier stream from dispersing into one another. Both convection and diffusion make significant
contributions to dispersion from approximately 320 s after the samples injection. This is the normal
time scale for a flow injection analysis. After approximately 25 s, diffusion is the only significant
contributor to dispersion, resulting in a flow profile similar to that shown in Figure 13.19d.

Figure 13.20 Illustration showing axial and radial diffusion. The blue band is the samples flow profile
and the red arrows indicate the direction of diffusion.

An FIA curve, or fiagram, is a plot of the detector's signal as a function of time. Figure 13.21 shows a
typical fiagram for conditions in which both convection and diffusion contribute to the sample's
dispersion. Also shown on the figure are several parameters for characterizing a sample's fiagram. Two
parameters define the time for a sample to move from the injector to the detector. Travel time, ta, is the
time between the sample's injection and the arrival of its leading edge at the detector. Residence time,
T, on the other hand, is the time required to obtain the maximum signal. The difference between the
residence time and travel time is t, which approaches zero when convection is the primary means of
dispersion, and increases in value as the contribution from diffusion becomes more important.

Figure 13.21 Typical fiagram for flow injection analysis showing the detector's response as a function of
time. See the text for an explanation of the parameters ta, t, t, T, T, and h.
The time required for the sample to pass through the detector's flow cell.and for the signal to return to
the baseline.is also described by two parameters. The baseline-to-baseline time, t, is the time between
the arrival of the sample's leading edge to the departure of its trailing edge. The elapsed time between
the maximum signal and its return to the baseline is the return time, T. The final characteristic
parameter of a fiagram is the sample's peak height, h.
Of the six parameters shown in Figure 13.21, the most important are peak height and return time. Peak
height is important because it is directly or indirectly related to the analyte's concentration. The
sensitivity of an FIA method, therefore, is determined by the peak height. The return time is important
because it determines the frequency with which we may inject samples. Figure 13.22 shows that if we
inject a second sample at a time T after injecting the first sample, there is little overlap of the two FIA
curves. By injecting samples at intervals of T, we obtain the maximum possible sampling rate.

Figure 13.22 Effect of return time, T, on sampling frequency.

Peak heights and return times are influenced by the dispersion of the sample's flow profile and by the
physical and chemical properties of the flow injection system. Physical parameters affecting h and T
include the volume of sample we inject, the flow rate, the length, diameter and geometry of the mixing
and reaction zones, and the presence of mixing points where separate channels merge together. The
kinetics of any chemical reactions between the sample and the reagents in the carrier stream also
influence the peak height and return time.
Unfortunately, there is no good theory that we can use to consistently predict the peak height and
return time for a given set of physical and chemical parameters. The design of a flow injection analyzer
for a particular analytical problem still occurs largely by a process of experimentation. Nevertheless, we
can make some general observations about the effects of physical and chemical parameters. In the
absence of chemical effects, we can improve sensitivitythat is, obtain larger peak heightsby injecting
larger samples, increasing the flow rate, decreasing the length and diameter of the tubing in the mixing
and reaction zone, and merging separate channels before the point where the sample is injected. With
the exception of sample volume, we can increase the sampling ratethat is, decrease the return time
using by the same combination of physical parameters. Larger sample volumes, however, lead to longer
return times and a decrease in sample throughput. The effect of chemical reactivity depends on whether
the species we are monitoring is a reactant or a product. For example, if we are monitoring a reactant,
we can improve sensitivity by choosing conditions that decrease the residence time, T, or by adjusting
the carrier streams composition so that the reaction occurs more slowly.
13D.2 Instrumentation
The basic components of a flow injection analyzer are shown in Figure 13.23 and include a pump for
propelling the carrier stream and reagent streams, a means for injecting the sample into the carrier
stream, and a detector for monitoring the composition of the carrier stream. Connecting these units is a
transport system that brings together separate channels and provides time for the sample to mix with
the carrier stream and to react with the reagent streams. We also can incorporate separation modules
into the transport system. Each of these components is considered in greater detail in this section.

Figure 13.23 Example of a typical flow injection analyzer showing the pump, the injector, the transport
system consisting of mixing/reaction coils and junctions, and the detector (minus the
spectrophotometer). This particular configuration has two channels: the carrier stream and a reagent
line.
Propelling Unit
The propelling unit moves the carrier stream through the flow injection analyzer. Although several
different propelling units have been used, the most common is a peristaltic pump. A peristaltic pump
consists of a set of rollers attached to the outside of a rotating drum, as shown in Figure 13.24. Tubing
from the reagent reservoirs fits between the rollers and a fixed plate. As the drum rotates the rollers
squeeze the tubing, forcing the contents of the tubing to move in the direction of the rotation.
Peristaltic pumps provide a constant flow rate, which is controlled by the drums speed of rotation and
the inner diameter of the tubing. Flow rates from 0.000540 mL/min are possible, which is more than
adequate to meet the needs of FIA where flow rates of 0.52.5 mL/min are common. One limitation to a
peristaltic pump is that it produces a pulsed flowparticularly at higher flow ratesthat may lead to
oscillations in the signal.

Figure 13.24 Schematic diagram of a peristaltic pump.

Injector
The sample, typically 5200 L, is injected into the carrier stream. Although syringe injections through a
rubber septum are possible, the more common methodas seen in Figure 13.23is to use a rotary, or
loop injector similar to that used in an HPLC. This type of injector provides for a reproducible sample
volume and is easily adaptable to automation, an important feature when high sampling rates are
needed.
Figure 12.39 and Figure 12.45 show examples of an HPLC loop injector.
Detector
The most commonly used detectors for flow injection analysis are the electrochemical and optical
detectors used in HPLC. These detectors are discussed in Chapter 12 and are not considered further in
this section. FIA detectors also have been designed around the use of ion selective electrodes and
atomic absorption spectroscopy.
Transport System
The heart of a flow injection analyzer is the transport system that brings together the carrier stream, the
sample, and any reagents that react with the sample. Each reagent stream is considered a separate
channel, and all channels must merge before the carrier stream reaches the detector. The complete
transport system is called a manifold.
The simplest manifold includes only a single channel, the basic outline of which is shown in Figure 13.25.
This type of manifold is commonly used for direct analyses that do not require a chemical reaction. In
this case the carrier stream serves only as a means for rapidly and reproducibly transporting the sample
to the detector. For example, this manifold design has been used for sample introduction in atomic
absorption spectroscopy, achieving sampling rates as high as 700 samples/h. A single-channel manifold
also is used for determining a samples pH or determining the concentration of metal ions using an ion
selective electrode.

Figure 13.25 Example of a single-channel manifold in which the reagent serves as the carrier stream and
as a species that reacts with the sample. The mixing/reaction coil is wrapped around a plastic cylinder.
We can also use the single-channel manifold in Figure 13.25 for systems in which we monitor the
product of a chemical reaction between the sample and a reactant. In this case the carrier stream both
transports the sample to the detector and reacts with the sample. Because the sample must mix with

the carrier stream, a lower flow rate is used. One example is the determination of chloride in water,
which is based on the following sequence of reactions.
Hg(SCN)2(aq) + 2Cl(aq) HgCl2(aq) + 2SCN(aq)
Fe3+(aq) + SCN(aq) Fe(SCN)2+(aq)
The carrier stream consists of an acidic solution of Hg(SCN)2 and Fe3+. Injecting a sample containing
chloride into the carrier stream displaces thiocyanate from Hg(SCN)2. The displaced thiocyanate then
reacts with Fe3+ to form the red-colored Fe(SCN)2+ complex, the absorbance of which is monitored at a
wavelength of 480 nm. Sampling rates of approximately 120 samples per hour have been achieved with
this system.18
Most flow injection analyses that include a chemical reaction use a manifold with two or more channels.
Including additional channels provides more control over the mixing of reagents and the interaction
between the reagents and the sample. Two configurations are possible for a dual-channel system. A
dual-channel manifold, such as the one shown in Figure 13.26a, is used when the reagents cannot be
premixed because of their reactivity. For example, in acidic solutions phosphate reacts with molybdate
to form the heteropoly acid H3P(Mo12O40). In the presence of ascorbic acid the molybdenum in the
heteropoly acid is reduced from Mo(VI) to Mo(V), forming a blue-colored complex that is monitored
spectrophotometrically at 660 nm.18 Because ascorbic acid reduces molybdate, the two reagents are
placed in separate channels that merge just before injecting the sample.
A dual-channel manifold can also be used to add a second reagent after injecting the sample into a
carrier stream, as shown in Figure 13.26b. This style of manifold is used for the quantitative analysis of
many analytes, including the determination of a wastewaters chemical oxygen demand (COD).19
Chemical oxygen demand is a measure of the amount organic matter in the wastewater sample. In the
conventional method of analysis, COD is determined by refluxing the sample for 2 h in the presence of
acid and a strong oxidizing agent, such as K2Cr2O7 or KMnO4. When refluxing is complete, the amount of
oxidant consumed in the reaction is determined by a redox titration. In the flow injection version of this
analysis, the sample is injected into a carrier stream of aqueous H2SO4, which merges with a solution of
the oxidant from a secondary channel. The oxidation reaction is kinetically slow. As a result, the mixing
and reaction coils are very long, typically 40 m, and submerged in a thermostated bath. The sampling
rate is lower than that for most flow injection analyses, but at 1030 samples/h it is substantially greater
than the redox titrimetric method.
You will find a more detailed description of the redox titrimetric method for COD in Chapter 9.

Figure 13.26 Two examples of a dual-channel manifold for flow injection analysis. In (a) the two
channels merge before the loop injector, and in (b) the two channels merge after the loop injector.
More complex manifolds involving three or more channels are common, but the possible combination of
designs is too numerous to discuss. One example of a four-channel manifold is shown in Figure 13.27.

Figure 13.27 Example of a four-channel manifold for a flow injection analysis.

Separation Modules
By incorporating a separation module in the flow injection manifold we can include a separation
dialysis, gaseous diffusion and liquid-liquid extractions are examplesinto a flow injection analysis.
Although these separations are never complete, they are reproducible if we carefully control the
experimental conditions.
Dialysis and gaseous diffusion are accomplished by placing a semipermeable membrane between the
carrier stream containing the sample and an acceptor stream, as shown in Figure 13.28. As the sample
stream passes through the separation module, a portion of those species capable of crossing the

semipermeable membrane do so, entering the acceptor stream. This type of separation module is
common for the analysis of clinical samples, such as serum and urine, where a dialysis membrane
separates the analyte from its complex matrix. Semipermeable gaseous diffusion membranes have been
used for the determination of ammonia and carbon dioxide in blood. For example, ammonia is
determined by injecting the sample into a carrier stream of aqueous NaOH. Ammonia diffuses across the
semipermeable membrane into an acceptor stream containing an acidbase indicator. The resulting
acidbase reaction between ammonia and the indicator is monitored spectrophotometrically.

Figure 13.28 Separation module for a flow injection analysis using a semipermeable membrane. The
smaller green solutes can pass through the semipermeable membrane and enter the acceptor stream,
but the larger blue solutes cannot. Although the separation is not completenote that some of the
green solute remains in the sample stream and exits as wasteit is reproducible if we do not change the
experimental conditions.
Liquidliquid extractions are accomplished by merging together two immiscible fluids, each carried in a
separate channel. The result is a segmented flow through the separation module, consisting of
alternating portions of the two phases. At the outlet of the separation module the two fluids are
separated by taking advantage of the difference in their densities. Figure 13.29 shows a typical
configuration for a separation module in which the sample is injected into an aqueous phase and
extracted into a less dense organic phase that passes through the detector.

Figure 13.29 Separation module for flow injection analysis using a liquidliquid extraction. The inset
shows the equilibrium reaction. As the sample moves through the equilibration zone, the analyte
extracts into the organic phase.

13D.3 Quantitative Applications

In a quantitative flow injection method a calibration curve is determined by injecting a series of external
standards containing known concentrations of analyte. The calibration curves formatexamples
include plots of absorbance versus concentration and potential versus concentrationdepends on the
method of detection. Calibration curves for standard spectroscopic and electrochemical methods are
discussed in Chapters 10 and 11 and are not considered further in this chapter.
Example 13.13 shows a typical example of a calibration curve for a flow injection analysis.
Flow injection analysis has been used to analyze a wide variety of samples, including environmental,
clinical, agricultural, industrial, and pharmaceutical samples. The majority of analyses involve
environmental and clinical samples, which is the focus of this section.
Quantitative analytical flow injection analytical methods have been developed for cationic, anionic, and
molecular pollutants in wastewater, freshwaters, groundwaters, and marine waters, three examples of
which we described in the previous section. Table 13.2 provides a partial listing of other analytes that
have been determined using FIA, many of which are modifications of standard spectrophotometric and
potentiometric methods. An additional advantage of FIA for environmental analysis is the ability to
provide for the continuous, in situ monitoring of pollutants in the field.20
The three examples are: the determination of chloride, the determination of phosphate, and the
determination of chemical oxygen demand.
Table 13.2 Selected Flow Injection Analysis Methods for Environmental Samples
analyte

sample

sample volume (L)

concentration range

sampling frequency (h1)

Ca2+

freshwater

20

0.87.2 ppm

80

Cu2+

groundwater

70700

100400 ppb

20

Pb2+

groundwater

70700

040 ppb

20

Zn2+

sea water

1000

1100 ppb

30-60

NH4+

sea water

60

0.1818.1 ppb

288

NO3

rain water

1000

110 ppm

40

SO42

fresh water

400

4140 ppm

180

CN

industrial

10

0.3100 ppm

40

Source: Adapted from Valcrcel, M.; Luque de Castro, M. D. Flow-Injection Analysis: Principles and
Practice, Ellis Horwood: Chichester, England, 1987.
As noted in Chapter 9, several standard methods for the analysis of water involve an acidbase,
complexation, or redox titration. It is easy to adapt these titrations to FIA using a single-channel
manifold similar to that shown in Figure 13.25.21 The titrantwhose concentration must be

stoichiometrically less than that of the analyteand a visual indicator are placed in the reagent
reservoir and continuously pumped through the manifold. When we inject the sample it thoroughly
mixes with the titrant in the carrier stream. The reaction between the analyte, which is in excess, and
the titrant produces a relatively broad rectangular flow profile for the sample. As the sample moves
toward the detector, additional mixing occurs and the width of its flow profile decreases. When the
sample passes through the detector, we determine the width of its flow profile, t, by monitoring the
indicators absorbance. A calibration curve of t versus log[analyte] is prepared using standard solutions
of analyte.
Flow injection analysis has also found numerous applications in the analysis of clinical samples, using
both enzymatic and nonenzymatic methods. Table 13.3 summarizes several examples.
Table 13.3 Selected Flow Injection Analysis Methods for Clinical Samples

analyte

sample

sample
volume (L)

concentration
range

sampling
frequency (h1)

nonenzymatic methods
Cu2+

serum

20

0.71.5 ppm

70

Cl

serum

60

50150 meq/L

125

PO43

serum

200

1060 ppm

130

total CO2

serum

50

1050 mM

70

chloropromazine

blood plasma

200

1.59 M

24

glucose

blood serum

26.5

0.515 mM

60

urea

blood serum

30

420 mM

60

ethanol

blood

30

530 ppm

50

enzymatic methods

Source: Adapted from Valcrcel, M.; Luque de Castro, M. D. Flow-Injection Analysis: Principles and
Practice, Ellis Horwood: Chichester, England, 1987.
The best way to appreciate the theoretical and practical details discussed in this section is to carefully
examine a typical analytical method. Although each method is unique, the following description of the
determination of phosphate provides an instructive example of a typical procedure. The description
here is based on Guy, R. D.; Ramaley, L.; Wentzell, P. D. An Experiment in the Sampling of Solids for
Chemical Analysis, J. Chem. Educ. 1998, 75, 10281033. As the title suggests, the primary focus of this
chapter is on sampling. A flow injection analysis, however, is used to analyze samples.

Representative Method 13.2

Determination of Phosphate by FIA
Description of Method
The FIA determination of phosphate is an adaptation of a standard spectrophotometric analysis for phosphate. In
the presence of acid, phosphate reacts with ammonium molybdate to form a yellow-colored complex in which
molybdenum is present as Mo(VI).
H3PO4(aq) + 12H2MoO4(aq) H3P(Mo12O40)(aq) + 12H2O(l)
In the presence of a reducing agent, such as ascorbic acid, the yellow-colored complex is reduced to a blue-colored
complex of Mo(V).
Procedure
Prepare the following three solutions: (a) 5.0 mM ammonium molybdate in 0.40 M HNO3; (b) 0.7% w/v ascorbic acid
in 1% v/v glycerin; and (c) 100.0 ppm phosphate standard using KH2PO4. Using the phosphate standard, prepare a
set of external standards with phosphate concentrations of 10, 20, 30, 40, 50 and 60 ppm. Use a manifold similar to
that shown in Figure 13.26a, placing a 50-cm mixing coil between the pump and the loop injector and a 50-cm
reaction coil between the loop injector and the detector. For both coils, use PTFE tubing with an internal diameter of
0.8 mm. Set the flow rate to 0.5 mL/min. Prepare a calibration curve by injecting 50 L of each standard, measuring
the absorbance at 650 nm. Samples are analyzed in the same manner.
Questions
1. How long does it take a sample to move from the loop injector to the detector?
The reaction coil is 50-cm long with an internal diameter of 0.8 mm. The volume of this tubing is
V = l r2 = 50 cm (0.08 cm / 2)2 = 0.25 cm3 = 0.25 mL
With a flow rate of 0.5 mL/min, it takes about 30 s for a sample to pass through the system.
2. The instructions for the standard spectrophotometric method indicate that the absorbance should be measured
510 min after adding the ascorbic acid. Why is this waiting period necessary in the spectrophotometric method, but
not necessary in the FIA method?
The reduction of the yellow-colored Mo(VI) complex to the blue-colored Mo(V) complex is a slow reaction. In the
standard spectrophotometric method it is difficult to reproducibly control the time between adding the reagents to
the sample and measuring the samples absorbance. To achieve good precision we allow the reaction to proceed to
completion before we measure the absorbance. As seen by the answer to the previous question, in the FIA method
the flow rate and the dimensions of the reaction coil determine the reaction time. Because this time is precisely
controlled, the reaction occurs to the same extent for all standards and samples. A shorter reaction time has the
advantage of allowing for a higher throughput of samples.
3. The spectrophotometric method recommends using phosphate standards of 210 ppm. Explain why the FIA
method uses a different range of standards.

In the FIA method we measure the absorbance before the formation of the blue-colored Mo(V) complex is
complete. Because the absorbance for any standard solution of phosphate is always smaller when using the FIA
method, the FIA method is less sensitive and higher concentrations of phosphate are necessary.
4. How would you incorporate a reagent blank into the FIA analysis?
A reagent blank can be obtained by injecting a sample of distilled water in place of the external standard or sample.
The reagent blanks absorbance is subtracted from the absorbances obtained for the standards and samples.

Example 13.13
The following data were obtained for a set of external standards when using Representative Method
13.2 to analyze phosphate in a wastewater sample.
[PO43] (ppm)

absorbance

10.00
20.00
30.00
40.00
50.00

0.079
0.160
0.233
0.316
0.482

What is the concentration of phosphate in a sample if it gives an absorbance of 0.287?

Solution
Figure 13.30 shows the external standards calibration curve and equation. Substituting in the samples
absorbance gives the concentration of phosphate in the sample as 36.1 ppm.

Figure 13.30 Calibration curve and equation for the data in Example 13.13.

13D.4 Evaluation
The majority of flow injection analysis applications are modifications of conventional titrimetric,
spectrophotometric, and electrochemical methods of analysis; thus, it is appropriate to compare FIA
methods to conventional methods. The scale of operations for FIA allows for the routine analysis of
minor and trace analytes, and for macro, meso, and micro samples. The ability to work with microliter
injection volumes is useful when the sample is scarce. Conventional methods of analysis usually have
smaller detection limits.
See Figure 3.5 to review the meaning minor and trace analytes, and the meaning of macro, meso and
micro samples.
The accuracy and precision of FIA methods are comparable to conventional methods of analysis. The
precision of a flow injection analysis is influenced by several variables that do not affect conventional
methods, including the stability of the flow rate and the reproducibility of the samples injection. In
addition, results from FIA may be more susceptible to temperature variations.
In general, the sensitivity of FIA is less than that for conventional methods of analysis for at least two
reasons. First, as with chemical kinetic methods, measurements in FIA are made under nonequilibrium
conditions when the signal has yet to reach its maximum value. Second, dispersion dilutes the sample as
it moves through the manifold. Because the variables that affect sensitivity are known, we can design
the FIA manifold to optimize the methods sensitivity.
Selectivity for an FIA method is often better than that for the corresponding conventional method of
analysis. In many cases this is due to the kinetic nature of the measurement process, in which potential
interferents may react more slowly than the analyte. Contamination from external sources also is less of
a problem because reagents are stored in closed reservoirs and are pumped through a system of
transport tubing that is closed to the environment.
Finally, FIA is an attractive technique when considering time, cost, and equipment. When using an
autosampler, a flow injection method can achieve very high sampling rates. A sampling rate of 20120
samples/h is not unusual and sampling rates as high as 1700 samples/h are possible. Because the
volume of the flow injection manifold is small, typically less than 2 mL, the consumption of reagents is
substantially smaller than that for a conventional method. This can lead to a significant decrease in the
cost per analysis. Flow injection analysis does require the need for additional equipmenta pump, a
loop injector, and a manifoldwhich adds to the cost of an analysis.
13E Summary and Problems
Contributors

David Harvey (DePauw University)