Green Synthesis of Silver Nano Particle Using Hibiscus Rosa Sinensis

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Green Synthesis of Silver Nano Particle

Using Hibiscus Rosa Sinensis
Article in IOSR Journal of Applied Physics January 2016
DOI: 10.9790/4861-0803023538
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3 authors:
Ambili Reveendran
Sanoj Varghese
Karpagam University
Karpagam University
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IOSR Journal of Applied Physics (IOSR-JAP)

e-ISSN: 2278-4861.Volume 8, Issue 3 Ver. II (May. - Jun. 2016), PP 35-38
www.iosrjournals.org
Green Synthesis of Silver Nano Particle Using Hibiscus Rosa

Sinensis
1
Ambili Reveendran , Sanoj Varghese , K.Viswanathan
3,*
Department of Physics, Karpagam University, Coimbatore, India
Abstract: Green synthesis of silver nanoparticles by the help of green plants is a very cost effective, safe, nontoxic, eco-friendly route of synthesis which can be used for the manufacture at a large scale. The formation of
the nanoparticle is identified by the colour change occurred in the solution of silver nitrate after the addition of
plant extract. This colour change is due to the property of quantum confinement which is a size dependent
property of nanoparticles which affects the optical property of the nanoparticles. The occurrence of the peak at
451 nm is due to the phenomenon of Surface Plasmon Resonance, which is due to the excitation of the surface
plasmons present on the outer surface of the silver nanoparticles due to the applied electromagnetic field. The
UV absorption peak at 451nm clearly indicates the formation of AgNPs. The absorption for the particular
wavelength was 0.331. SEM studies were helpful at describing their morphology and distribution.
Keywords: Green Synthesis, Silver Nano particles, Hibiscus Rosa Sinensis
I.
Introduction
Biological synthesis of nanoparticle is a challenging concept which is very well known as green
synthesis. Among the different biological agents, plants provide safe and beneficial way to the synthesis of
metallic nanoparticle as it is easily available, so there are possibilities for large scale production. In recent years,
metallic nano particles have great attention because of modification of properties perceived due to size effects,
distribution and morphology (Elizondo et al 2011). Silver nano particles have particular interest due to its
unique properties such as high conductivity, chemical stability, catality and antibacterial.
Hibscus adalah tanaman herba evergreen (gambar 1). Ini memiliki bunga hias, besar, merah gelap.
Bunga kembang sepatu, akar dan daun adalah sifat anodyne dan emmenagogue. Mereka mengatur haid dan
menstimulasi sirkulasi darah. Ekstrak bunga telah secara tradisional digunakan untuk gangguan hati, tekanan
darah tinggi dan sebagai afrodisiak. Muda daun dan bunga digunakan dalam kasus sakit kepala. Rebusan daun,
akar, dan buah-buahan sangat membantu dalam perawatan rematik, bisul dan batuk, dan buah yang digunakan
secara Kandenkaniath
eksternal dalam Viswanathan
kasus keseleo, luka dan bisul. Hibiscus teh ini kaya Vitamin C. kembang sepatu rosa
University
sinensisKalasalingam
memilih untuk
sintesis partikel nano perak karena adanya phytochemical yang memberikan alami
caping dan agen pereduksi (Yebpella et al. 2011)
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Fig.1. Hibiscus Rosa Sinensis
AllDOI:
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Green Synthesis of Silver Nano Particle Using Hibiscus Rosa Sinensis

The aim of this work is to use Hibiscus rosa sinensis leaves extract as a low cost and eco friendly
approach to green synthesis of silver nanoparticles. The silver nano particle have been characterised by UV
Visible spectroscopy and SEM.
II.
Materials And Methods
2.1. Materials
Silver Nitrate (AgNO3, 99% purity, Merck Products), hibiscus leaves collected from Kerala
2.2. Preparation of the plant extract
The leaves of Hibiscus rosa simensis plant were collected from the local garden and then washed
thoroughly with tap water to remove the dust and dirt particles. The leaves are then dried under shade for about
four days and finely powdered. About 2 grams of the powder was taken and mixed with distilled water. The
aqueous leaf extract was taken and then filtered using wattmen filter paper to obtain pure hibiscus leaves extract
with pale green colour to be used as reducing and capping agents in AgNP synthesis. .
Fig. 3 SEM of Hibiscus Silver Nitrate Extract
2.3. Preparation of Silver Nitrate Solution
0.125g of Silver nitrate was added into 100ml of distilled water and stirred continuously for 1-2min to
get Silver Nitrate solution.
2.4. Silver nano particle synthesis
The best volume of plant extract were added to the best molarity of AgNO 3 solution at room
temperature and stirred continuously for ten minutes using Magnetic Stirrer. Slow reduction takes place and
kept for 24 hours to obtain the colour change. After 24 hours pale green colour changes to red colour, which
indicate the formation of silver nano particle. The Hibiscus leaves extract and AgNO 3 solution mixture was then
characterised using UV and SEM.
III.
Analysis Method
3.1 UV Visible absorption

Silver nano particles were synthesized by reducing silver metal ions solution with Hibiscus leaf extract
were initially characterized using UV-Visible Spectrophotometer (Shimadzu BL ).
3.2 Scanning Electron Microscope(SEM)
Particle size and its distribution were assessed with Scanning Electron Microscope. Electron interacts
with the electrons in the sample, producing various signals that can be detected and that contain information
about surface topography and composition of the samples.
IV.
Result And Discussion
Fig. the
4 SEM
of Hibiscus
Nitrate
Extract
The present study involves
green
synthesisSilver
of silver
nanoparticles
using widely available plant,
Hibiscus rosa sinensis, as its reducing agent. While there has been numerous methods to synthesize nano
V. synthesis,
Conclusion
particles like Chemical synthesis, Microwave
gas phase methods and sol-gel processing in which
A
simple
green
synthesis
of
stable
silver
nano
particle
using
Hibiscuss
rosa sinensis
at room
most of them are expensive and hazardous. Plant extract
provide
better
platform
for nanoparticles
synthesis
as
temperature
was
reported
in
this
study.
The
synthesis
was
found
to
be
efficient
in
terms
of
reaction
time
as well
they are free from toxic chemicals.
as stability of synthesised nano particles which excluded chemical agents. The green synthesis of silver nano
particle
provides simple environmental ecofriendly and cost effective route for the synthesis of nano
4.1. UVmethods
Visible spectroscopy
particle. It
The
of nanoparticle
identified
change
colour of
of nanoparticles
Hibiscus leaf using
extractgreen
and
hasformation
been widely
used as an was
important
toolbyto the
detect
the of
presence
characterized
Visibleofspectroscopy.
synthesis and by
theUV
stability
metal nanoparticle in aqueous solution. In particular, absorbance in the range of
420 to 500nm has been used as an indicator to confirm the reduction of Ag+ to metallic Ag. Fig. (2) shows UV
spectrum
ofReference
Hibiscus
Silverthe
Nitrate
Extract peak of Silver Nano Particle
Visible spectra of Hibiscus- Fig.2
SilverUV
nitrate
extract.
In this study,
absorbance
[1]
Daisy
Philip,
Physics
E
42(2010)
Green
synthesis
of
gold
and
silver
nano
particle
using
hibiscus
roasa sinensis.
occurs at 451nm which is a narrow peak with an increase in absorbance due
to increase
in number of nano
[2]
Ahamed
AElectron
Moosa,
Ali
Mousa
Ridha andofMustafa
Al-Kaser,
Process
parameters
for solution.
green synthesis of silver nno particle using
4.2.
Scanning
Microscopy
particles
formed
as a result
of reduction
silver ions
present
in the
aqueous
leaves extract of Aloe Vera Plant, IJMCR, (2015), ISSN 2321-3124.
SEM analysis was used to determine the structure of reaction product that were formed. In this study
[3]
Sharma, V.K.Yngard, R.a & Lin, Silver nano particle; green synthesis and their antimicrobial activitie, ACIS 145(1-2), (2009),
the SEM
image (Fig. 3 and Fig. 4) has showed individual silver particles as well as particle agglomeration. This
pp(83-86).
indicates,
the S,particle
sizeH,isMirmohammadi
irregular and SV,
to some
extent
sphericalof silver nanoparticles: chemical, physical and biological
[4]
Iravani
Korbekandi
Zolfaghari
B, Synthesis
[5]
[6]
[7]
methods. Research in Pharmacuitical Sciences, (2014); 9(6): 385406

Elzondo.N et al, Green Chemistry-Environmentally begin approaches; Shanghai, China;) Green synthesis of silver and gold nano
particles. (2011), intech pp 139-156
P.Saravanan et al, Synthesis and characterisation of Nano materials; Defefnce science journal , 58(4), (2008), PP 504-516
Jae Yong Song, Beom Soo Kim, Rapid biological synthesis of Silver rnanoparticle using plant leaf extract; Bioprocess Biosysting
engineering, 32 (2009) PP (79-84)
DOI: 10.9790/4861-0803023538
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See discussions, stats, and author profiles for this publication at: https://www.researchgate.

Green Synthesis of Silver Nano Particle

Spectroscopic studies of Human Blood View project

Light scattering studies of cancer tissues View project

IOSR Journal of Applied Physics (IOSR-JAP)

Green Synthesis of Silver Nano Particle Using Hibiscus Rosa

Ambili Reveendran , Sanoj Varghese , K.Viswanathan

Department of Physics, Karpagam University, Coimbatore, India

Fig.1. Hibiscus Rosa Sinensis

Green Synthesis of Silver Nano Particle Using Hibiscus Rosa Sinensis

Materials And Methods

3.1 UV Visible absorption

Result And Discussion

methods. Research in Pharmacuitical Sciences, (2014); 9(6): 385406

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