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    Growth Rate and OUR Relation - 24.11.16

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    AthonioMourinho
    The document describes a study where a biochemical engineer measured oxygen uptake rate (OUR) and biomass concentration (X) during antibiotic fermentation. To implement her findings, expressions are derived to estimate X and growth rate (dX/dt) from OUR and time data, assuming a simple yield and maintenance model. Values for the yield parameter (YX/O2) are then calculated from the experimental data by determining the maximum growth rate, plotting OUR vs. X, and calculating the slope.

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    Growth Rate and OUR Relation - 24.11.16

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    AthonioMourinho
    499 views3 pages
    The document describes a study where a biochemical engineer measured oxygen uptake rate (OUR) and biomass concentration (X) during antibiotic fermentation. To implement her findings, expressions are derived to estimate X and growth rate (dX/dt) from OUR and time data, assuming a simple yield and maintenance model. Values for the yield parameter (YX/O2) are then calculated from the experimental data by determining the maximum growth rate, plotting OUR vs. X, and calculating the slope.

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    Growth Rate problems

    Original Title

    Growth Rate and OUR Relation_24.11.16

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    The document describes a study where a biochemical engineer measured oxygen uptake rate (OUR) and biomass concentration (X) during antibiotic fermentation. To implement her findings, expressions are derived to estimate X and growth rate (dX/dt) from OUR and time data, assuming a simple yield and maintenance model. Values for the yield parameter (YX/O2) are then calculated from the experimental data by determining the maximum growth rate, plotting OUR vs. X, and calculating the slope.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    499 views3 pages

    Growth Rate and OUR Relation - 24.11.16

    Uploaded by

    AthonioMourinho
    The document describes a study where a biochemical engineer measured oxygen uptake rate (OUR) and biomass concentration (X) during antibiotic fermentation. To implement her findings, expressions are derived to estimate X and growth rate (dX/dt) from OUR and time data, assuming a simple yield and maintenance model. Values for the yield parameter (YX/O2) are then calculated from the experimental data by determining the maximum growth rate, plotting OUR vs. X, and calculating the slope.

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    Problem (Shuler and Kargi, pp 202)

    Time OUR (g/h) X (g/l)


    A biochemical engineer has determined in her laboratory that the 1 0,011 0,6
    optimal productivity of a valuable antibiotic is achieved when the 2 0,008 0,63
    carbon nutrient (molasses) is metered into the fermenter at a rate 3 0,084 0,63
    4 0,153 0,76
    proportional to the growth rate. However, she cannot implement 5 0,198 1,06
    her discovery in the antibiotic plant, since there is no reliable way 6 0,273 1,56
    to measure the growth rate (dX/dt) or biomass concentration (X) 7 0,393 2,23
    during the course of fermentation. It is suggested that an oxygen 8 0,493 2,85
    9 0,642 4,15
    analyzer be installed on the plant fermenters so that the OUR 10 0,915 5,37
    (oxygen uptake rate, g/l-h) may be measured. 11 1,031 7,59
    12 1,12 9,4
    1. Derive expressions that may be used to estimate X and 13 1,37 11,4
    14 1,58 12,22
    dX/dt from OUR and time data, assuming that a simple
    15 1,26 13
    yield and maintenance model may be used to describe the 16 1,58 13,37
    rate of oxygen consumption by the culture. 17 1,26 14,47
    18 1,12 15,37
    2. Calculate values for the yield(YX/O2) parameter from the 19 1,2 16,12
    20 0,99 16,18
    data 21 0,86 16,67
    22Sandip Bankar 0,9 17,01
    Derive expressions that may be used to estimate X and dX/dt from OUR and time data, assuming that a
    simple yield and maintenance model may be used to describe the rate of oxygen consumption by the culture.
    X
    OUR = + mO2 X
    YX
    O2

    O2 used for growth O2 used for maintenance

    1 dX
    = substitute into above equation
    X dt
    1 dX
    OUR = + mO2 X
    YX dt
    O2
    1 dX
    To estimate dX
    : In exponential growth phase O2 for growth dominates, hence OUR
    dt YX dt
    O2

    To estimate X: During stationary phase, cells aren't growing and O2 is needed for maintenance only OUR mO X
    2

    Sandip Bankar
    Calculate values for the yield(YX/O2) parameter from the data
    Calculate max (Plot graph lnX Vs t (linear range 3-10h) and max. slope is =?)

    In exponential growth phase OUR


    1 dX or OUR =
    X
    YX dt YX
    O2 O2


    Plot OUR Vs X graph and calculate slope = = ? gO2/g.dw.hr
    YX
    O2
    Calculate YX YX =

    O2 O2 slope

    Sandip Bankar

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