BB @ 618 nm

Molarity (mol L-1) Absorbance

23.2 0.638
18.6 0.524 0.7
13.9 0.393 0.6 f(x) = 0
9.28 0.273
0.5
4.64 0.137

Absorbance
0.4
0.3
0.2
0.1
BB @ 434 nm 0
Molarity (mol L-1) Absorbance 0 5
23.2 0.124 Ab
18.6 0.092
0.14
13.9 0.06
0.12
9.28 0.04
0.1 f(x) = 0.00495118
4.64 0.016 R = 0.991629369

Absorbance
0.08
BT=0.005
*
0.06
0.04
0.02
0
0 5

Molarity (mo

TB @ 434 nm
Molarity (mol L-1) Absorbance
61.6 0.831
12.3 0.151
37 0.472 0.9
24.6 0.31 0.8
49.3 0.647 f(x) = 0.013176
0.7
0.6
*TT=0.0132
Absorbance

0.5
0.4
0.3
0.2
0.1
0
0 10 20
 0.4

Absorb
0.3
0.2
0.1
0
0 10 20

TB @ 618 nm
Molarity (mol L-1) Absorbance
61.6 0.122 A
49.3 0.113
0.14
37 0.104 f(x) = 0.0023357
24.6 0.081 0.12 R = 0.96442803
12.3 0.047 0.1

Absorpton
0.08
*TB=0.00230 0.06
0.04
0.02
0
0 10 20

AT,0.534@434 AB,0.427@618
Molarity (mol L-1) Absorbance of Unknown (Measured)
370 0.376
380 0.417
390 0.456
400 0.494
410 0.523
420 0.533
430 0.534
434 0.53
440 0.521
450 0.49
460 0.451
470 0.404
480 0.354
490 0.304
500 0.263
510 0.236
520 0.215
530 0.214
540 0.222
550 0.241
560 0.267
570 0.301
580 0.341
590 0.384
600 0.416
610 0.434
618 0.427
620 0.424
630 0.373
640 0.289
650 0.192
660 0.12
670 0.069
 Absorbance BB at 618 nm
0.7
0.6 f(x) = 0.0279984777x

0.5
Absorbance

0.4
0.3
0.2
0.1
0
0 5 10 15
Absorbance BB at 434 nm
Molarity (mol L-1)
0.14
0.12
0.1 f(x) = 0.0049511838x
R = 0.9916293697
Absorbance

0.08 Absorbance
0.06 Linear (Abso
0.04
0.02
0
0 5 10 15 20 25

Molarity (mol L-1)

Absorbance TB at 434 nm
0.9
0.8
f(x) = 0.0131769355x
0.7
0.6
Absorbance

0.5
0.4
0.3
0.2
0.1
0
0 10 20 30 40 50 60 7
Molarity (mol L-1)
 0.4

Absorb
0.3
0.2
0.1
0
0 10 20 30 40 50 60 7
Molarity (mol L-1)

Absorbance TB AT 618 nm
0.14
f(x) = 0.0023357643x
0.12 R = 0.9644280365
0.1
Absorpton

0.08 Absorban
0.06 Linear (Ab
0.04
0.02
0
0 10 20 30 40 50 60 70
Molarity (mol L-1)

Adjusted BB Adjusted TB BB TB
0.0861172414 0.2811599026 0.16 0.515
0.1028024569 0.3106407468 0.191 0.569
0.111414181 0.3401215909 0.207 0.623
0.1124906466 0.3750618506 0.209 0.687
0.1022642241 0.4072724026 0.19 0.746
0.0839643103 0.4318397727 0.156 0.791
0.0737378879 0.4394829545 0.137 0.805
0.0678173276 0.4394829545 0.126 0.805
0.062435 0.4367532468 0.116 0.8
0.0548997414 0.4132777597 0.102 0.757
0.0581291379 0.3794293831 0.108 0.695
0.0575909052 0.3379378247 0.107 0.619
0.060282069 0.2882571429 0.112 0.528
0.0629732328 0.2402142857 0.117 0.44
0.0699702586 0.1943551948 0.13 0.356
0.0769672845 0.1577771104 0.143 0.289
0.0904231034 0.1206530844 0.168 0.221
0.1103377155 0.0988154221 0.205 0.181
0.1329434914 0.0873506494 0.247 0.16
0.1490904741 0.0731561688 0.277 0.134
0.1883814655 0.0813452922 0.35 0.149
 0.2185225 0.0818912338 0.406 0.15
0.251892931 0.0851668831 0.468 0.156
0.2890309914 0.088988474 0.537 0.163
0.3202484914 0.0884425325 0.595 0.162
0.3433925 0.0813452922 0.638 0.149
0.3514659914 0.0666048701 0.653 0.122
0.3509277586 0.065512987 0.652 0.12
0.3191720259 0.0447672078 0.593 0.082
0.2508164655 0.0251133117 0.466 0.046
0.17169625 0.013648539 0.319 0.025
0.107108319 0.0070972403 0.199 0.013
0.060282069 0.0027297078 0.112 0.005
B at 618 nm

Absorbance
Linear (Absorbance)

15 20 25

Absorbance
Linear (Absorbance)

60 70
 Linear (Absorbance)

60 70

Absorbance of Unknown (Theoretical)

0.367277144
0.413443204 Absorban
0.451535772
0.487552497 0.9
0.509536627
0.515804083
0.8
0.513220842
0.507300282
0.499188247 0.7
0.468177501
0.437558521 0.6
0.39552873
0.348539212
0.303187518 0.5
Absorbance

0.264325453
0.234744395 0.4
0.211076188
0.209153138
0.220294141 0.3
0.222246643
0.269726758 0.2

0.1

0
340 390 440 490 540
 0.3

0.2
0.300413734
0.337059814
0.1
0.378019465
0.408691024
0.424737792 0
340 390 440 490 540
0.418070862
0.416440746 Wavelength (nm)
0.363939234
0.275929777
0.185344789
0.114205559
0.063011777
 Absorbance vs Wavelength

Absorbance of Unknown (Measured)

Adjusted BB
Adjusted TB
BB
TB
Absorbance of Unknown (Theoretical)

440 490 540 590 640 690

 Absorbance of Unknown (Theoretical)

440 490 540 590 640 690

Wavelength (nm)
 Absorbance BB at 618 nm
0.7

0.6 f(x) = 0.0279984777x

0.5

0.4
Absorbance

Absorbance
0.3 Linear (Absorbance)

0.2

0.1

0
0 5 10 15 20 25
Molarity (mol L-1)

Absorbance BB at 434 nm
0.14

0.12
f(x) = 0.0049511838x
0.1 R = 0.9916293697

0.08
Absorbance

Absorbance
0.06 Linear (Absorbance)

0.04

0.02

0
0 5 10 15 20 25
Molarity (mol L-1)
 Absorbance TB at 434 nm
0.9

0.8
f(x) = 0.0131769355x
0.7

0.6

0.5
Absorbance

Absorbance
0.4 Linear (Absorbance)

0.3

0.2

0.1

0
0 10 20 30 40 50 60 70

Molarity (mol L-1)

Absorbance TB AT 618 nm
0.14
f(x) = 0.0023357643x
R = 0.9644280365
0.12

0.1

0.08
Absorpton

Absorbance
Linear (Absorbance)
0.06

0.04

0.02

0
0 10 20 30 40 50 60 70

Molarity (mol L-1)

 Absorbance vs Wavelength
0.9

0.8

0.7

0.6
Absorbance of Unknown (Measured)
0.5 Adjusted BB
Absorbance

Adjusted TB
BB
0.4 TB
Absorbance of Unknown (Theoretical)

0.3

0.2

0.1

0
340 390 440 490 540 590 640 690

Wavelength (nm)