Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Scribd Logo
Upload

Welcome to Scribd!

  • 160 views

    Bilirubin Total ®vanadate ©

    Uploaded by

    rizal_aspan
    This document provides instructions for using a total bilirubin reagents kit for quantitative determination of total bilirubin in human serum and plasma. The kit uses a two-endpoint vanadate method where bilirubin reacts with vanadic acid to form biliverdin, decreasing absorbance at 450nm proportional to bilirubin concentration. The kit contains citrate buffer and sodium metavanadate reagents, has a linear range of 0-684umol/L, and requires strict control of temperature, time, and mixing for accurate results.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd

    Bilirubin Total ®vanadate ©

    Uploaded by

    rizal_aspan
    160 views1 page
    This document provides instructions for using a total bilirubin reagents kit for quantitative determination of total bilirubin in human serum and plasma. The kit uses a two-endpoint vanadate method where bilirubin reacts with vanadic acid to form biliverdin, decreasing absorbance at 450nm proportional to bilirubin concentration. The kit contains citrate buffer and sodium metavanadate reagents, has a linear range of 0-684umol/L, and requires strict control of temperature, time, and mixing for accurate results.

    Original Description:

    tetap

    Original Title

    Bilirubin_Total£®Vanadate£©

    Copyright

    © © All Rights Reserved

    Available Formats

    DOC, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides instructions for using a total bilirubin reagents kit for quantitative determination of total bilirubin in human serum and plasma. The kit uses a two-endpoint vanadate method where bilirubin reacts with vanadic acid to form biliverdin, decreasing absorbance at 450nm proportional to bilirubin concentration. The kit contains citrate buffer and sodium metavanadate reagents, has a linear range of 0-684umol/L, and requires strict control of temperature, time, and mixing for accurate results.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    160 views1 page

    Bilirubin Total ®vanadate ©

    Uploaded by

    rizal_aspan
    This document provides instructions for using a total bilirubin reagents kit for quantitative determination of total bilirubin in human serum and plasma. The kit uses a two-endpoint vanadate method where bilirubin reacts with vanadic acid to form biliverdin, decreasing absorbance at 450nm proportional to bilirubin concentration. The kit contains citrate buffer and sodium metavanadate reagents, has a linear range of 0-684umol/L, and requires strict control of temperature, time, and mixing for accurate results.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    You are on page 1of 1

    Total Bilirubin (TB) Reagents Kit 2.

    Incomplete mixture of specimen and reagents results in


    APPLICABLE INSTUMENT inaccurate assay. Always mix thoroughly before assay.
    (Vanadate Method) For full-automated biochemistry analyzer. 3. All pipettes and instruments should be calibrated before
    use.
    4. To ensure the accuracy of results, reaction temperature and
    SPECIMEN time period should be controlled strictly in assay.
    5. Reagents storage and validity please see the package insert.
    Fresh serum, or plasma. 6. Please refer to the local legal requirements for waste
    management.
    PROCEDURES 7. This kit is for professionals and in vitro diagnostic use only.
    INTENDED USE
    Test Mode: two end point method;
    For the quantitative determination of total bilirubin in human Temperature: 37;
    serum and plasma Cuvette: 1.0cm;
    Wavelength: 450nm;
    Specimen: 10 ul;
    Specification R-1: 280, R-2: 70ul;
    Reaction: down
    150ml/box R-160ml2; R-215ml2
    Test step
    Addition Blank tube Standard tube Test tube
    PRINCIPAL Distilled water 7 L
    Under the particular term pH3.0 Bilirubin can react with Standard 7 L
    Sample 7 L
    vanadic acid to form biliverdine in serumThen the absorbance of R-1 210 L 210 L 210 L
    bilirubin at 450nm decreases and is proportional to concentration in Mixing and incubate for 3-5mins under 37test each
    Tube A2A=A2A1
    blood serum.. Therefore the total bilirubin concentration in the R-2 70 L 70L 70 L
    sample can be obtained by measuring the absorbance before and
    CALCULATIONS
    after the vanadate oxidation. A specific
    T.Bilimol/L= Standard Conc.
    Vanadic Acid A standard
    Bilirubin Biliverdin
    REFERENCE RANGE
    5.020.1mol/L0.31.2mg/dl
    It is suggested that each lab establish individual reference
    REAGENTS range.
    Component Conc.
    PRODUCT PERFORMANCE
    R1 Citrate buffer 100 mmol/L 1. Precision: Within run: CV4.0; Inter run: CV5.0
    R2 Sodium metavanadate 10 mmol/L 2. Linearity: 0684mol/L

    PRECAUTIONS
    STORAGE 1. Can change the specimen and reagent volume based on
    Stable for 24 months, direct exposure to sunshine avoided. analyzer requirement.

    Labnovation Technologies, Inc. 1F, W2-B Bldg., Keji 2 Rd. South, Hi-Tech Industrial Park, Shenzhen 518057 Tel: +86 755 86368328 Fax: +86 755 86368318 www.labnovation.com Rev. Aug. 09

    You might also like