Fermentation of Agro-Based Waste and Residues From Different Sectors: A Review
Fermentation of Agro-Based Waste and Residues From Different Sectors: A Review
Fermentation of Agro-Based Waste and Residues From Different Sectors: A Review
In India the economic importance of food and food processing sector is dominating. But the environmental
issues like wastes or by-products of food industry sector is an alarming problem today. In food processing and packaging
industry about one third of the food is spoilt before it reaches the consumers. This shows a huge economic loss in food
sector. There are certain methods of dumping into the ground, incineration of agricultural waste and using the ash in
agricultural lands. But this methods causes environmental degradation and polluting the environment. Dumping the
agro-waste under normal ground causes decomposition and foul smell. Again the incineration if carried out without
proper technique causes pollution. Hence, degrading the environment. So, one of the major scientific technique is
Review Article
carrying out different type of fermentation processes such as solid state fermentation and submerged state fermentation
which could be helpful to convert this wastage into some useful high value products with the help of living cells or
microorganisms in a controlled optimal atmosphere inside a specially designed suitable bioreactor or fermenter and
following a suitable recovery process the final product is recovered and purified.
KEYWORDS: Fermentation, Agro-Wastes, Solid State Fermentation, Liquid State Fermentation, Substrates,
Microorganisms
Received: Feb 02, 2017; Accepted: Mar 21, 2017; Published: Mar 25, 2017; Paper Id.: IJASRAPR201754
INTRODUCTION
Food processing and packaging sector contributes to a huge agricultural wastage. These wastes causes
different pollution problems on disposal in the ground and a huge loss of valuable biomass and nutrients is
observed. Different sectors included in food industry is fruits and vegetables processing, oil seed, sugar, milk and
milk products, tannery, textile and packaging industries. From these sectors during many unit operation processes
a huge loss of nutrient is there in form of solid wastes like fruit peels, seeds, skin, bran, hey, whey, wood and
paper pulp, fish gills, scales and liquid wastes as sugar syrups, molasses, fruit juice are wasted.
(Pandey et al.1999). Food wastes from the food processing industry contains many nutrients in form of
carbohydrates, proteins, lipids, suspended particles according to the high bio-chemical oxygen demand and
chemical oxygen demand (Jayathilakan 2012) which is used by the microorganisms in the fermenter as their
nutrients to grow and carry on the multiplication and fermentation process.
Concept of Fermentation
The basic concept of fermentation is to facilitate the rapid escalation, multiplication and growth of the
target organisms in the nutrients provided as substrates. The desirable microorganisms metabolises the present
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426 Sonali Biswal & Manasi Ray
sugar in the substrate into chemicals like ethanol (alcohol), lactic acids. (Hui et al. 2004). Enzymes are protein which is
synthesized as intra and extra cellular compounds. Enzymes energize and catalyze biochemical reaction with high
specificity and enhance the reaction rate (Renge et al. 2012).
Type of Fermentation
Depending on the state of substrate the fermentation can be classified into Solid state fermentation and Liquid or
submerged fermentation. In solid state fermentation the solid wastes which can be easily recycled can be directly used as
substrate as high nutrient medium for growth of target microorganism. Whereas in liquid fermentation the free flowing
liquid wastes from processing industries like molasses and broths are used as the nutrient medium. The solid substrates has
an advantage that it can be used slowly and steadily upto a long period of time by the organisms, while the liquid substrates
get easily and rapidly used up hence time to time supplementation of nutrient is required which is time consuming. While
the solid state fermentation is carried out where the target organism needs less moisture content such as fungi, the liquid
fermentation is suitable for high moisture activity microorganisms like bacteria. But the liquid fermentation gives
advantage of easy purification and recovery after the fermentation process and is used where the product is needed in
liquid form. (Subramaniyam and Vimal 2012).Each type of fermentation has its own advantages.
The selection of substrate for the fermentation depends on many factors such as the nature of target organism, the
nature of product to be generated. Cost and availability of the substrate also plays an important role as for high volume,
low cost product the substrate should not be plentyly available in low cost. Particle size should also be considered, the
smaller particle size gives a good surface area for the proliferation of the target microorganisms but if the size is too small
then proper enzyme action cannot occur hence resulting in poor growth of the organism resulting to incomplete
fermentation and less product formation. Likewise large size particles doesnt give more surface area, though aeration and
respiration is accelerated in this case. (Suganthi et al. 2011).The moisture content of the substrate is also required as per the
target organism. When the target oraganism is bacteria more moisture substrate is required than in case of fungi which
require less moisture to grow. The product nature determines the selection of the substrate as well as the target
microorganisms.
Factors affecting fermentation includes a number of important parameter which are needed to be taken care of
during the process. Temperature, pH level, level of dissolved oxygen and dissolved carbon dioxide inside the substrate,
cooling of the vessel with cold water is maintained throughout the fermentation process. The chemical composition of the
substrate (whether it is in solid or liquid/submerged state),type of operation(i.e. batch, fed-batch or continuous), type of
feeding inlet, type of mixer or agitator blades, power of the agitator motor, shear rate are some other factors which are the
factors which should be maintained else its variation leads to different problems affecting the quality and quantity of the
product by affecting its taste, smell, appearance and texture. Sometimes different toxins are created which leads to
difficulty in purification.
Various useful components are extracted by using different suitable fermentation method according to the waste
substrates available. These components are of many commercial uses and have high economic values. Many bioactive
compounds such as antibiotics (Maragkoudakis et al., 2009; Saykhedkar and Singhal, 2004; Ohno, 1995), pigments
(Dharmaraj et al., 2009), enzymes (Aguilar et al., 2008; Kokila and Mrudula 2010), hypercholestrolemic agents
(Xie and Tang 2007; Pansuriya and Singhal, 2010), antioxidants (Tafulo et al., 2010), antihypertensive agents
(Nakahara et al., 2010), antitumor agents (Ruiz-Sanchez et al., 2010), biosurfactants and bioactive peptides
(Pritchard et al., 2010) have been extracted using fermentation.
Fermenter
A fermenter also known as bioreactor is generally used in a vast field of biochemical and pharmaceutical industry.
A fermenter is a specially designed vessel or container made of stainless steel, glass or non-corrosive material in which
residues and wastes from different agricultural sector such as peels, seeds, outer covering of fruits and vegetables, fish
wastes such as gills, scales which are rich in protein, paper and wood pieces, sugar molasses and bagasses etc are
converted into products of some use. The optimum temperature, pH and other suitable environment is created, maintained
and controlled inside the reactor by different devices so that to encourage the growth of required specific microorganisms.
All bioreactors have heterogeneous systems which deal with two or more phases like solid, liquid and gas. Sterilisation of
the substrate and the bioreactor is yet another important factor which is needed to be taken care of so as to stop the growth
of unwanted microorganisms other than the required ones. In a fermenter the low value waste substrates are converted and
used into high value products by utilising living microorganisms or enzymes.
Fermenters are of different capacities depending on various factors. The design and capacities depends on the
specific target microorganism, the optimal temperature, pH and other optimal parameters required for the growth of the
target microorganisms and the quantity and quality of product. Another factor is the monetary investment and cost of
running and maintenance. Bioreactors or fermenters can vary from shake conical flask (100-1000 ml), in laboratory the
fermenter ranges from (1-50 L) or more, for pilot scale level (0.3- 10 m3) to plant scale (2-500 m3) depending on quality
and quantity of the high value product. For products like alcoholic beverages, and lactic acids production an simple
running fermenter is required without much asceptic conditions maintenance as the recovered product is of large volume
and low value. For high value and low volume products such as single cell protein, antibiotics more asceptic condition is
maintained and more systematic operation is required (Jagani et al.2010).The diagram of a fermenter showing different
components is shown in the figure 1.
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428 Sonali Biswal & Manasi Ray
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430 Sonali Biswal & Manasi Ray
Desired products are separated and purified after successful fermentation or enzyme reactions Investment in
recovery equipment is high. Isolation costs represent a good proportion (sometimes up to 60%) of the cost of the final
product. In one antibiotic factory, recovery equipment cost four times more than the fermenter. The necessity of having a
well-planned and reliable recovery process and an efficient recovery plant is therefore of utmost importance.
CONCLUSIONS
The food industry witness a huge loss of the products in form of peels, crushes, seeds etc during different
processing and packaging processes. The food waste products are must be utilised. Fermentation is process involving
microbial growths, which is widely accepted in bio chemical and pharmaceutical industry is now used in the food industry
to ferment the by-products of the food industry as the substrates to carry the growth of required microorganisms into mane
useful products like acids, alcohols, antibiotics etc. These products are commercially useful and add to the economy of
food industry.
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