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    Light Cycler System For Swine Influenza Virus Detection: Real Time Ready Influenza A/H1N1 Detection Set

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    yasmine
    The document describes a Light Cycler System for detecting swine influenza virus. It can detect both influenza A using the matrix protein 2 gene as well as the new H1N1 strain using the hemagglutinin gene. The system allows for parallel testing of both targets using validated primer/probe mixes. It involves extracting the viral RNA, reverse transcribing it to cDNA, and then real-time PCR detection on the Light Cycler platform. The system provides a simple, fast and reliable method for detecting influenza A and the 2009 H1N1 pandemic strain.

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    Light Cycler System For Swine Influenza Virus Detection: Real Time Ready Influenza A/H1N1 Detection Set

    Uploaded by

    yasmine
    25 views2 pages
    The document describes a Light Cycler System for detecting swine influenza virus. It can detect both influenza A using the matrix protein 2 gene as well as the new H1N1 strain using the hemagglutinin gene. The system allows for parallel testing of both targets using validated primer/probe mixes. It involves extracting the viral RNA, reverse transcribing it to cDNA, and then real-time PCR detection on the Light Cycler platform. The system provides a simple, fast and reliable method for detecting influenza A and the 2009 H1N1 pandemic strain.

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    Original Title

    LC H1N1 Brosur

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    The document describes a Light Cycler System for detecting swine influenza virus. It can detect both influenza A using the matrix protein 2 gene as well as the new H1N1 strain using the hemagglutinin gene. The system allows for parallel testing of both targets using validated primer/probe mixes. It involves extracting the viral RNA, reverse transcribing it to cDNA, and then real-time PCR detection on the Light Cycler platform. The system provides a simple, fast and reliable method for detecting influenza A and the 2009 H1N1 pandemic strain.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    25 views2 pages

    Light Cycler System For Swine Influenza Virus Detection: Real Time Ready Influenza A/H1N1 Detection Set

    Uploaded by

    yasmine
    The document describes a Light Cycler System for detecting swine influenza virus. It can detect both influenza A using the matrix protein 2 gene as well as the new H1N1 strain using the hemagglutinin gene. The system allows for parallel testing of both targets using validated primer/probe mixes. It involves extracting the viral RNA, reverse transcribing it to cDNA, and then real-time PCR detection on the Light Cycler platform. The system provides a simple, fast and reliable method for detecting influenza A and the 2009 H1N1 pandemic strain.

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    of 2

    Light Cycler System

    for Swine Influenza Virus Detection

    Influenza A is a negative strand single-stranded RNA virus


    from the Orthomyvirus family which infects birds and
    mammals. It is characterized using the Hemagglutinin (HA)
    and Neuraminidase (NA) genes. In April 2009, a new H1N1
    virus was reported in Mexico. This H1N1 influenza virus has
    a NA gene similar to H5N1, but a very specific HA gene
    which can be used for its identification. RT-PCR detection of
    influenza A is based on the conserved matrix protein 2 gene
    (M2). The respective subtype identification is based on
    detection of the hemagglutinin HA1 gene. A network of
    virologist has selected and verified two primer/hydrolysis
    probe mixes to detect the viral RNA genes of influenza using
    actual H1N1 clinical samples.
    The recommended procedure for testing for the new
    Influenza pandemic (H1N1) 2009 is the detection of the M2
    gene for Influenza A in combination with an extraction
    control from human nucleic acid. Positive samples are then
    tested targeting specific H1 gene, or parallel testing of both
    targets is performed.
    The primer/probe set for detection of Influenza A
    Hemagglutinin HA1 (Panning et al., 2009, submitted) has

    Real Time Ready Influenza A/H1N1 Detection Set


    Assay Principle

    Test specific for Test specific for


    Influenza A New H1N1

    Target: Matrix Protein 2 Target: Hemagglutinin


    According to recommendations of According to recommendations of
    the WHO / CDC the Robert Koch Institute,
    Both tests can run in parallel

    Influenza A/H1N1 Detection


    Workflow
    Simple, speedy, secure
    Manual or
    Wash or automated sample Automated
    preparation virus

    1 min + 25 min
    + 55 min
    Reagents Information
    The Influenza A Virus is an RNA
    Virus
    Biological sample extraction kit
    Manual sample preparation: High Pure Viral Nucleic Acid Isolation Kit (Cat. No. 11
    858874001)
    Automated sample prep. : Magna Pure Compact Nucleic Acid Isolation Kit ( Cat. No.
    03730972001)
    Reverse Transcription
    One Step RT-PCR: RealTime Ready RNA Virus Master (Cat. No. 05 619416001)
    Two Step RT-PCR: Light Cycler 480 Probes Master (Cat. No. 04707494001)
    Light Cycler TaqMan Master (Cat. No. 04535286001 or 04735536001)
    Transcriptor First Strand cDNA Synthesis Kit (Cat. No. 04 379012001)
    Thermal Cycler
    Light Cycler 2.0 and Light Cycler 480
    Amplification Specific Reagents and PCR protocols

    Influenza A/H1N1 Detection Set/RNA Vírus Master


     For the detection of Influenza A Matrix Protein 2 gene M2 and the specific H1 gene
     Assay characteristic and advantages: highly specific, very sensitive (10-100 copies),
    internal extraction
    control (human gene)
     Good performance of RNA Master and Detection Set

    M2 H1

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