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    Micro-Nano System Engineering Laboratory: Lab Manual

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    nadiashining_star_56
    The document provides instructions for using a stereo microscopic, including how to observe specimens, adjust settings like focus and magnification, and capture and measure images using software. Key parts of the microscope are identified, such as the eyepieces, stage plate, and zoom adjustment knobs. Steps for using the microscope include placing a specimen on the stage and adjusting settings to view it clearly. The document also outlines how to mount a camera, capture images using software, and calibrate and measure saved images.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Micro-Nano System Engineering Laboratory: Lab Manual

    Uploaded by

    nadiashining_star_56
    20 views15 pages
    The document provides instructions for using a stereo microscopic, including how to observe specimens, adjust settings like focus and magnification, and capture and measure images using software. Key parts of the microscope are identified, such as the eyepieces, stage plate, and zoom adjustment knobs. Steps for using the microscope include placing a specimen on the stage and adjusting settings to view it clearly. The document also outlines how to mount a camera, capture images using software, and calibrate and measure saved images.

    Original Description:

    manual lab

    Original Title

    2- Stereo Microscopic

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    The document provides instructions for using a stereo microscopic, including how to observe specimens, adjust settings like focus and magnification, and capture and measure images using software. Key parts of the microscope are identified, such as the eyepieces, stage plate, and zoom adjustment knobs. Steps for using the microscope include placing a specimen on the stage and adjusting settings to view it clearly. The document also outlines how to mount a camera, capture images using software, and calibrate and measure saved images.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    20 views15 pages

    Micro-Nano System Engineering Laboratory: Lab Manual

    Uploaded by

    nadiashining_star_56
    The document provides instructions for using a stereo microscopic, including how to observe specimens, adjust settings like focus and magnification, and capture and measure images using software. Key parts of the microscope are identified, such as the eyepieces, stage plate, and zoom adjustment knobs. Steps for using the microscope include placing a specimen on the stage and adjusting settings to view it clearly. The document also outlines how to mount a camera, capture images using software, and calibrate and measure saved images.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 15

    MICRO-NANO SYSTEM

    ENGINEERING LABORATORY

    LAB MANUAL

    STEREO MICROSCOPIC
    INTRODUCTION
     For three dimensional perspective (3D).
     For viewing relatively large and solid surfaces or specimens.
     For detailed work such as microsurgery, watch making and circuit
    board inspection and manufacturing.

    APPARATUS
     Stereo Microscopic
     Specimen
     PC, Software
    SIDE VIEW OF STEREO MICROSCOPIC
     Right View of Stereo Microscopic  Left View of Stereo Microscopic
    NOMENCLATURE DESCRIPTIONS
    Zoom adjustment knob Left/right single-shaft horizontal knobs
    (with high/low zoom magnification
    stopper)
    Camera Port Install and uninstall the camera
    Light path selector lever • Binocular Observation – Push the lever
    • Camera Observation – Pull the lever

    Power Supply To switch ON/OFF light.

    Focus adjustment knob The magnification can be limited in the


    desired range by setting each stopper on
    the microscope body (also used as the
    magnification indices) and the stopper ring
    on each zoom adjustment knob. The right
    zoom adjustment knob is used to limit the
    higher end of the magnification, and the
    left knob is used to limit the lower end.
    X and Y axis knob To move the specimen to a desired
    position, rotate the X-axis knob and Y-axis
    knob.
    NOMENCLATURE DESCRIPTIONS
    Eyepiece/ Observation Tube To observed the objective.

    Microscope Body · SZ61


    · SZ51
    · SZ61-60
    · SZ51-60
    · SZ61TR
    Stage Plate When reflected light illumination is
    used, the stage plate can be placed
    either the white or black surface
    facing up depending on which side
    makes the specimen easier to view.
    • ESD performance, use the black
    surface
    • Transmitted light illumination, use
    transparent glass stage plate.
    X and Y axis knob To move the specimen to a desired
    position, rotate the X-axis knob and
    Y-axis knob.
    Observation Procedure

    START

    Put the specimen on


    the stage center plate

    Turn ON power

    Adjust the interpupillary


    distance.
    • Observed the specimen from the
    Adjust the eyepiece diopter.
    eyepieces 2
    • Rotate the zoom adjustment knob 3 to
    Observed the
    the target magnification and rotate the
    specimen from the
    focus adjustment knob 4 to bring the
    eyepieces
    specimen into accurate
    • (Please refer to the next page for
    GOOD figure)
    NO

    YES
    END
    INSTRUCTION FIGURE
    Adjust the interpupillary distance 1

    Adjust the eyepiece diopter.

    • Observed the specimen from the


    eyepieces 2
    • Rotate the zoom adjustment knob
    t 3 to the target magnification
    and rotate the focus adjustment
    knob 4 to bring the specimen
    into accurate
    Mounting the TV Camera

    1. Using the Allen wrench, loosen the C-mount clamping


    screw 1 and remove the C-mount seat 2.
    2. Screw the C-mount seat 2 into the TV camera 3.
    3. Place the C-mount seat in the original position and tighten
    the clamping screw 1.
    4. Connect the cables and monitor to the TV camera.
    Procedure To Capture Image Using Software
    analySIS getIT- Step By Step

    1.Double click on icon analySIS getIT


    2.Click on icon (a) and the DigiAcquis TWAIN window would appear.
    d a c

    3. Click on icon (b) to capture the image.

    4. Click on icon (c) to set the correct scale bar / magnification


    for the image.
    5. To burn the scale on the image, click on “Image”, then “Scale Bar” then
    “Burn into Image”.

    6. Click on icon (d) to save the image to the desired folder.

    Note: It is compulsory to save the file as type Tagged Image Format “tif” for
    the images which require measurement in the future.
    measureIT – Step By Step
    1. Close the analySIS getIT application.
    2. Double click on icon measureIT.
    3. Click on icon (e) to apply the scale bar. The scale bar will be accurate if
    the image was saved as “tif” file.
    4. Click on icon (f) to do measurement.
    5. Click on icon (g) for some annotation on the image.

    e f g
    CALIBRATION
    BY DIFFERENCE ZOOMING
    0.6X 1.2X

    1.5X 1.0X
    2.5X 3.5X

    2.0X 3.0X
    4.5X 4.0X
    Prepared by: Approved by:

    ……………………………………. …………………………………….
    Nurul Nadiah Binti Bakri Dr. Mohd Ridzuan Ahmad
    Research Assistance (RA) Dept. of Control and Mechatronics Engineering,
    Dept. Control and Mechatronics Engineering Faculty of Electrical Engineering,
    Faculty of Electrical Engineering UTM, Skudai.
    UTM, Skudai.

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