Agilent Cary 60 UV-Vis

Efficient. Accurate. Flexible.

Guaranteed specifications

Introduction
The Agilent Cary 60 UV-Vis spectrophotometer is efficient, accurate and flexible, and is
designed to meet both current and future measurement needs. The proven, robust design of
the Cary 60 comprises a double beam, Czerny-Turner monochromator, 190–1100 nm
wavelength range, 1.5 nm fixed spectral bandwidth, full spectrum Xenon pulse lamp single
source with exceptionally long life, dual silicon diode detectors, quartz overcoated optics,
scan rates up to 24,000 nm/min, 80 data points/sec maximum measurement rate, non-
measurement phase stepping wavelength drive, room light immunity, central control by PC
with Microsoft® Windows® operating system. Supported by GLP software, optional 21 CFR
Part 11 capable software, and dedicated instrument validation software which includes
pharmacopeia test suites.
Agilent Cary 60 UV-Vis spectrophotometers are manufactured according to a quality
management system certified to ISO 9001. The guaranteed specifications are listed in this
document and are based on the 4 sigma statistical confidence level of the final acceptance
tests performed at the factory.
Features, advantages and Feature Advantage/Benefit
benefits Xenon pulse lamp source Eliminates photobleaching while allowing the highest quality data to be
collected over the complete UV-Vis range — all at the lowest cost of
ownership due to an exceptionally long lamp life.
Room light immunity Unique optical design allows accurate sample measurement even with
the sample lid open — especially useful for enzyme assays, fiber-optic
based measurements or high throughput QA/QC labs.

1.5 nm fixed spectral Gives excellent spectral resolution for solids and liquids and meets
bandwidth international Pharmacopoiea compliance regulations.

190–1100 nm wavelength Complete coverage of UV-Vis range and extending up into the NIR.
range
24,000 nm/min maximum Allows complete spectral range scanning in under 3 seconds, ideal for
scan rate fast kinetics or high sample throughput.

80 data points/second Allows accurate measurement of sub-second kinetic reactions with

maximum measurement excellent data fitting.
rate
Photometric range up to Permits the analysis of highly turbid solutions and a wide range of
4 Abs sample concentrations (optical densities), as well as reducing sample
preparation (dilution) requirements.

Non-measurement phase Means that sample and reference measurements are made at the same
stepping wavelength drive wavelength ensuring that no peak shifts are observed — even when
measuring at the fastest scan speeds.
Focused beam measuring Ensures efficient energy coupling to accessories including fiber optic
1.5 x 1.0 mm probes and ultra-microvolume cuvettes for measurement of low volume
samples.
Instrument hardware
Source

Unique full-spectrum Xenon flash lamp (80 Hz) with typical lifetime of
10 years (guaranteed 3 years)

Monochromator

Czerny-Turner

Grating

Holographic, 27.5 x 35 mm, 1200 lines/mm, blaze angle 8.6° at 240 nm

Beam splitting system

Beam splitter

Detectors

2 silicon diode detectors for simultaneous sample beam and reference

beam measurements
Optical design

Double beam Czerny-Turner monochromator

UV-Vis limiting resolution (nm)

≤ 1.5 nm

Toluene/hexane limiting resolution (EP/BP and TGA test)

≥ 1.5

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Agilent Cary 60 UV-Vis guaranteed specifications

Instrument hardware Stray light (%T)

At 198 nm (12 g/L KCl, TGA & BP/EP method) ≤ 1%

At 220 nm (10 g/L NaI ASTM method) ≤ 0.05%
At 370 nm (50 mg/L NaNO2) ≤ 0.05%

Wavelength range (nm)

190–1100 nm

Wavelength accuracy (nm)

± 0.5 at 541.94 nm

Wavelength reproducibility (nm)

± 0.1 nm

Photometric accuracy (Abs)

Using NIST 930E filters at 1 Abs ± 0.005 Abs

At 0.2, 0.5 & 0.75 Abs (14.2% w/v KNO3, TGA method) ± 0.01 Abs
0.292 to 0.865 Abs (60.06 mg/L K2Cr2O7, BP method) ± 0.01 Abs

Photometric range (Abs)

± 4.0 Abs

Photometric display

± 9.9999 Abs, ± 200.00 %T

Photometric reproducibility (Abs)

Using NIST 930E filters, at 465 nm, 2 s SAT

Maximum deviation at 1 Abs < 0.004 Abs
Standard deviation for 10 measurements < 0.00050 Abs
Using NIST 930E filters, at 546.1 nm, 2 s SAT
Maximum deviation at 0.5 Abs < 0.003 Abs
Standard deviation for 10 measurements < 0.0030 Abs

Photometric stability (Abs/hour)

500 nm, 10 s SAT < 0.0004 Abs

Photometric noise (Abs, RMS)

500 nm, 1 s SAT At 0 Abs < 0.0001 Abs

At 1 Abs < 0.0005 Abs
At 2 Abs < 0.005 Abs
260 nm, 1 s SAT At 0 Abs < 0.00015 Abs

Baseline flatness (Abs)

200 to 850 nm, smooth 21 filter applied, baseline corrected ± 0.001 Abs

Compartment size (width x depth x height)

130 mm x 523 mm x 123 mm

Note that sample compartment can be left open during measurement
due to room light immunity of Cary 60

Sample compartment access

Top and front

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Recommended environmental Instrument dimensions (width x depth x height)
conditions
Packed 595 x 710 x 350 mm (24 x 28 x 14 in)
Unpacked 477 x 567 x 196 mm (19 x 23 x 8 in)
The Cary 60 has been designed to withstand the weight of a PC
monitor up to 10 kg (33 lb)

Instrument weight

Packed 23 kg (51 lb), Unpacked 18 kg (40 lb)

Instrument conditions

Condition Altitude (m, ft) Temp. (°C, °F) Humidity (%RH) non-
condensing
Non-operating (transport) 0–4600, 0–15000 -40–75 °C, -40–167 °F 15–90%
Operating within 0–3100, 0–10000 5–40 °C, 41–104 °F 50–80%
performance
specifications
For optimum analytical performance, it is recommended that the ambient temperature of the
laboratory be between 20–25 °C and be held constant to within ±2 °C throughout the entire working
day

Instrument electrical requirements

A standard 3.2 A/12 V plug pack is provided. Power cords are provided
based on the user’s country requirements. Only the supplied power
supply is to be used with this equipment.
Required supply voltage 100–240 V AC, Frequency 47-63 Hz
Nominal rating Scanning: 18 W, Idle: 9 W

Operational Spectral bandwidth (nm)

Fixed at 1.5 nm (approximately)

Signal averaging (seconds)

0.0125–999 s

Maximum scan rate (nm/min)

24,000 nm/min

Slew rate (nm/min)

24,000 nm/min

Data interval (nm)

0.15–5.0 nm

Repetitive scanning

4800 data points per minute, maximum number of cycles: 999,

maximum cycle time (min): 9999

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Agilent Cary 60 UV-Vis guaranteed specifications

Operational Data collection rate

80 data points/second

Temperature monitor

Temperature probe inside cuvette (using the Temperature Probe

Accessory)

Minimum sample volume

0.5 μL
Customer support policies Support and training

Agilent is renowned for providing expert applications and service

support. Agilent has a global network of factory-trained specialists
ready to provide support for hardware, software, or applications
wherever you are located. Services include:
• Full 12-month warranty support
• Seven (7) year hardware support period from date of last unit
manufacture. After this time, parts and supplies will be provided if
available.
• Preventive maintenance to deliver consistent operation and minimize
downtime
• Troubleshooting, maintenance and repair
• Software support services
• Compliance services including IQ and OQ of hardware and software
• Comprehensive warranty extension and service contracts, including
peripherals
• Classroom training and onsite training delivered by experts

Further details More information

For further information please consult your Agilent office or supplier, or

our website at www.agilent.com

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www.agilent.com/chem
Agilent shall not be liable for errors contained herein or for incidental or consequential
damages in connection with the furnishing, performance or use of this material.

Information, descriptions, and specifications in this publication are subject to change

without notice.

© Agilent Technologies, Inc. 2011

Published December 7, 2011
Publication number: 5990-7881EN