Water Quality Worksheet

Name: _______________________________
Date: _____________________ Core: _____
Water Quality
For aquatic organisms, both plants and animals, a sudden or drastic change in their environment can be life-
threatening, so testing the quality of water in terms of temperature, pH, oxygen levels, and nitrates is
essential in determining the overall health of a water source. Monthly monitoring and frequent observations
are necessary in order to sustain hearty ecosystems and make sure pollution is not becoming a problem or
excess nutrients are not disrupting the balance. Described below are some water quality factors that can
easily be assessed to find out how a water system is doing.
Temperature
There are many factors that can affect water temperature causing it to increase or decrease. Shallow water
tends to be the temperature of its surroundings…warm if in a sunny area, cooler if shaded. Thermal
pollution (the release of hot water from factories) and run-off from hot asphalt can increase water
temperatures dramatically. Water temperatures outside the “normal” range can be harmful to aquatic plants
and animals by lowering their resistance to pollutants, diseases, and parasites.
Many aquatic organisms prefer cooler water than warmer water. Cooler water is more dense, so it is found in
deeper waters. Warmer water is less dense, so it is found closer to the surface. Why do organisms prefer the
cooler waters? The temperature of the water plays a key role in the amount of dissolved gases like oxygen or
carbon dioxide. As water gets colder, more oxygen can be dissolved in it. Organisms that require more
oxygen will be found in deeper parts of the water because it is plentiful down there. Aquatic plants behave a
little differently and like to float on top of or be close to the surface of the water. Warmer water and
exposure to sunlight will increase the amount of photosynthesis aquatic plants and algae perform.
Unfortunately, too much photosynthesis will lead to excessive plant growth and algal blooms.
Directions: Based on what you read…identify the effects from the causes of increasing and decreasing
temperatures.
Possible Causes Possible Effects

Increasing Shallow water
Temps Warmer air temperature
Direct sunlight
Thermal pollution
Warm run-off
Decreasing Deep water
Temps Cooler air temperature
Shady
Name: _______________________________
Date: _____________________ Core: _____
Dissolved Oxygen (DO)

As you well know by now, oxygen is a necessity for respiration, and respiration is a life process of all living
things. So, how does oxygen get into the water in order to be used by aquatic organisms for their survival?
Oxygen gas dissolves in water in a variety of ways. Some oxygen will naturally diffuse into the water
directly from the air. Photosynthesis by aquatic plants, algae and phytoplankton will contribute large
amounts of oxygen. Of course they do this by removing the carbon dioxide produced primarily by aquatic
animals…part of a cycle called the O2/CO2 cycle.
Dissolved oxygen will also change as a result of an algal bloom. While the algae are growing at a fast rate,
the DO levels will increase. As the resources get used up, space, light, and CO2 become limited, the algae
will start to die off. As a result, bacteria will begin to rapidly decompose the dying/dead algae, a process
requiring lots of oxygen. Thus the before mentioned abundance of DO, suddenly becomes depleted. This
phenomenon is known as eutrophication and is usually caused by an excess of nutrients from fertilizer run-
off to begin with. DO levels can become so low after this that many other aquatic organisms begin to die off
as well because they don’t have enough oxygen for survival. Ultimately, oxygen gas is vital to the existence
of most aquatic organisms and contributes to a greater diversity of living things when abundant and plentiful.
Directions: Based on what you read…identify the effects from the causes of increasing and decreasing
oxygen levels.

Increasing Cooler water
DO Photosynthesis is
occurring
Algal bloom (initial)
Decreasing Warmer water

DO Night time (no
photosynthesis)
Excess decomposition
Name: _______________________________
Date: _____________________ Core: _____
pH
When water mixes with certain substances, the bonds between the oxygen and hydrogen atoms can be broken
into hydrogen (H+) or a hydroxide (OH-) ions. When this happens, water can become more alkaline (basic)
or acidic. The concentration of hydrogen or hydroxide ions determines water’s pH value and is assessed
using a scale of 1—14. When the concentration of these two ions is equal, water has a pH of 7…what we
call neutral. More H+ ions decrease the pH, making the water more acidic. More OH- ions increase the pH,
making the water more alkaline or basic.
The pH of water may be affected by rainfall, which is slightly acidic because it carries with it dissolved
carbon dioxide (carbonic acid) and other pollutants. However, run-off that travels over certain minerals such
as limestone on its way to a stream or river can cause the pH to become more alkaline. Other things that can
affect pH are photosynthesis (uses up CO2), decomposition (creates CO2), respiration (creates CO2) and
excretion (creates ammonia). As you can see in the graphic above, aquatic organisms can be greatly affected
by sudden changes in the water’s pH.
Directions: Based on what you read…identify the causes of increasing and decreasing pH levels.

Increasing Plants will not grow
pH Harmful or lethal to fish if too basic
(alkaline)
Decreasing Fish eggs will not hatch

pH Harmful or lethal to fish, frogs, and insects
(acidic) if too acidic
Name: _______________________________
Date: _____________________ Core: _____
Nitrates and Nitrites
Nitrogen is a necessity of life used to build proteins. Even though most of the air in the atmosphere is made
up of nitrogen gas, plants and animals have no way to use it or convert it into the usable form of
nitrogen…nitrites and nitrates. Fortunately, bacteria, through decomposition of waste and dead or decaying
matter, “fix” the nitrogen for other living things through a process called the nitrogen cycle.
Waste
*Nitrosomonas and Nitrospira are types of bacteria
Certain bacteria in the water and gravel change nitrogen in the ammonia of waste products into nitrites and
nitrates. Plants diffuse nitrates into their roots/leaves so they can be used by the plants to build proteins.
Then animals will eat plants to get their needed nitrogen for protein production. Animals create waste and/or
organisms die which will be decomposed and the cycle starts all over. This of course is grossly simplified,
but it highlights the basic principles of the nitrogen cycle.
One other way nitrates enter an aquatic environment is from agricultural run-off containing fertilizers or
excess nutrients. Nitrates are relatively harmless to freshwater fish, but they will also act as a fertilizer for
plant growth. An excess of nitrates will allow more photosynthesis to take place in plants and algae, thus
leading to eutrophication. Algae will blanket the entire surface of the water and the plants that grow
underneath the water will not get enough light and die. Decomposition ensues and all the oxygen is used up
causing other organisms to die too. So, although the nitrates themselves are harmless, their presence can
have devastating effects.
Reflection: Answer the following questions in complete sentences and use information from above to
support your answer.
1) Suppose the nitrate level rose significantly for several weeks. How would the plants and animals be
affected? How might water quality change?
2) Sally was very excited about her new fish tank. In one evening, she filled it with water, treated the water
to remove harsh chemicals, added a couple plants and several fish. Within 2 weeks, everything in her tank
died. Based on what you read above…what might be the cause of Sally’s tank of death?

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Name: _______________________________

Date: _________________ Core: _

Possible Causes Possible Effects

Dissolved Oxygen (DO)

Possible Causes Possible Effects

Decreasing Warmer water

Possible Causes Possible Effects

Decreasing Fish eggs will not hatch

*Nitrosomonas and Nitrospira are types of bacteria

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