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    Antibody Datasheet

    Uploaded by

    Michael Daley
    This product datasheet describes an anti-Dopamine D2 Receptor antibody that is a rabbit polyclonal antibody targeting the C-terminal region of the human Dopamine D2 Receptor. It is suitable for use in immunohistochemistry and western blot applications in rat, human and other species. Images demonstrate labeling of the receptor in rat brain and other tissues. The antibody is supplied as a liquid for use at dilutions between 0.1-2 μg/ml depending on the application.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Antibody Datasheet

    Uploaded by

    Michael Daley
    41 views4 pages
    This product datasheet describes an anti-Dopamine D2 Receptor antibody that is a rabbit polyclonal antibody targeting the C-terminal region of the human Dopamine D2 Receptor. It is suitable for use in immunohistochemistry and western blot applications in rat, human and other species. Images demonstrate labeling of the receptor in rat brain and other tissues. The antibody is supplied as a liquid for use at dilutions between 0.1-2 μg/ml depending on the application.

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    Antibody Datasheet

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    This product datasheet describes an anti-Dopamine D2 Receptor antibody that is a rabbit polyclonal antibody targeting the C-terminal region of the human Dopamine D2 Receptor. It is suitable for use in immunohistochemistry and western blot applications in rat, human and other species. Images demonstrate labeling of the receptor in rat brain and other tissues. The antibody is supplied as a liquid for use at dilutions between 0.1-2 μg/ml depending on the application.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    41 views4 pages

    Antibody Datasheet

    Uploaded by

    Michael Daley
    This product datasheet describes an anti-Dopamine D2 Receptor antibody that is a rabbit polyclonal antibody targeting the C-terminal region of the human Dopamine D2 Receptor. It is suitable for use in immunohistochemistry and western blot applications in rat, human and other species. Images demonstrate labeling of the receptor in rat brain and other tissues. The antibody is supplied as a liquid for use at dilutions between 0.1-2 μg/ml depending on the application.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Product datasheet

    Anti-Dopamine D2 Receptor antibody - C-terminal


    ab191041
    4 Images

    Overview

    Product name Anti-Dopamine D2 Receptor antibody - C-terminal


    Description Rabbit polyclonal to Dopamine D2 Receptor - C-terminal
    Host species Rabbit
    Tested applications Suitable for: IHC-Fr, IHC-P, WB
    Species reactivity Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Chimpanzee
    Immunogen Synthetic peptide corresponding to Human Dopamine Receptor D2L aa 360-375 (C terminal).
    Sequence:
    RRKLSQQKEKKATQML

    Database link: P14416


    Run BLAST with Run BLAST with
    Positive control Rat brain tissue lysate, SKOV and SW620 cell lysates. Rat brain tissue.

    Properties

    Form Liquid
    Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
    term. Avoid freeze / thaw cycle.
    Storage buffer Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium
    phosphate
    Purity Immunogen affinity purified
    Clonality Polyclonal
    Isotype IgG

    Applications

    Our Abpromise guarantee covers the use of ab191041 in the following tested applications.
    1
    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes

    IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

    IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC
    staining protocol.

    WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 51 kDa.

    Target

    Function This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is
    mediated by G proteins which inhibit adenylyl cyclase.
    Involvement in disease Defects in DRD2 are associated with dystonia type 11 (DYT11) [MIM:159900]; also known as
    alcohol-responsive dystonia. DYT11 is a myoclonic dystonia. Dystonia is defined by the presence
    of sustained involuntary muscle contractions, often leading to abnormal postures. DYT11 is
    characterized by involuntary lightning jerks and dystonic movements and postures alleviated by
    alcohol. Inheritance is autosomal dominant. The age of onset, pattern of body involvement,
    presence of myoclonus and response to alcohol are all variable.
    Sequence similarities Belongs to the G-protein coupled receptor 1 family.
    Cellular localization Cell membrane.

    Images

    All lanes : Anti-Dopamine D2 Receptor antibody - C-terminal


    (ab191041) at 0.5 µg/ml

    Lane 1 : Rat brain tissue lysate


    Lane 2 : SKOV cell lysate
    Lane 3 : SW620 cell lysate

    Predicted band size: 51 kDa

    Western blot - Anti-Dopamine D2 Receptor antibody


    - C-terminal (ab191041)

    2
    Immunohistochemical staining of paraffin-embedded Rat brain
    tissue labeling Dopamine Receptor D2L using ab191041 at 1
    µg/ml.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-


    embedded sections) - Anti-Dopamine D2 Receptor
    antibody - C-terminal (ab191041)

    Immunohistochemical staining of frozen Rat small intestine tissue


    labeling Dopamine Receptor D2L using ab191041 at 2 µg/ml.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-


    embedded sections) - Anti-Dopamine D2 Receptor
    antibody - C-terminal (ab191041)

    3
    Immunohistochemical staining of frozen Rat brain tissue labeling
    Dopamine Receptor D2L using ab191041 at 2 µg/ml.

    Immunohistochemistry (Frozen sections) - Anti-


    Dopamine D2 Receptor antibody - C-terminal
    (ab191041)

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Our Abpromise to you: Quality guaranteed and expert technical support

    Replacement or refund for products not performing as stated on the datasheet


    Valid for 12 months from date of delivery
    Response to your inquiry within 24 hours

    We provide support in Chinese, English, French, German, Japanese and Spanish


    Extensive multi-media technical resources to help you
    We investigate all quality concerns to ensure our products perform to the highest standards

    If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
    please visit https://www.abcam.com/abpromise or contact our technical team.

    Terms and conditions

    Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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