DO EZO Datasheet

V 5.
3
Revised 8/13/19
EZO-DO
Embedded Dissolved Oxygen Circuit
™
Reads Dissolved Oxygen

0.01 − 100+ mg/L
Range
0.1 − 400+ % saturation
Accuracy +/– 0.05 mg/L
Response time 1 reading per sec
Supported probes Any galvanic probe
Calibration 1 or 2 point
Temperature, salinity
and pressure compensation Yes
Data protocol UART & I2C
Default I2C address 97 (0x61)
Operating voltage 3.3V − 5V
Data format ASCII
PATENT PROTECTED
Written by Jordan Press
Designed by Noah Press This is an evolving document, check back for updates.
This is sensitive electronic equipment. Get this device working in
a solderless breadboard first. Once this device has been soldered
it is no longer covered by our warranty.
This device has been designed to be soldered and can be soldered

at any time. Once that decision has been made, Atlas Scientific no
longer assumes responsibility for the device’s continued operation.
The embedded systems engineer is now the responsible party.
Get this device working in a Do not embed this device without

solderless breadboard first! testing it in a solderless breadboard!
1 5 10 15 20 25 30
A A
B B
C C
D D
E E
F F
G G
H H
I I
J J
1 5 10 15 20 25 30
r 0.1
Table of contents
Circuit dimensions 4 Correct wiring 8
Power consumption 4 Calibration theory 9
Absolute max ratings 4 Preserve calibration solution 12
Operating principle 5 Default state 13
Power and data isolation 6 Available data protocols 14
UART IC
2
UART mode 16 I2C mode 43
Receiving data from device 17 Sending commands 44
Sending commands to device 18 Requesting data 45
LED color definition 19 Response codes 46
UART quick command page 20 LED color definition 47
LED control 21 I2C quick command page 48
Find 22 LED control 49
Continuous reading mode 23 Find 50
Single reading mode 24 Taking reading 51
Calibration 25 Calibration 52
Export calibration 26 Export calibration 53
Import calibration 27 Import calibration 54
Temperature compensation 28 Temperature compensation 55
Salinity compensation 29 Salinity compensation 56
Pressure compensation 30 Pressure compensation 57
Enable/disable parameters 31 Enable/disable parameters 58
Naming device 32 Device information 59
Device information 33 Reading device status 60
Response codes 34 Sleep mode/low power 61
Reading device status 35 Protocol lock 62
Sleep mode/low power 36 I2C address change 63
Change baud rate 37 Factory reset 64
Protocol lock 38 Change to UART mode 65
Factory reset 39 Manual switching to UART 66
Change to I2C mode 40
Manual switching to I2C 41
Circuit footprint 67
Datasheet change log 68
Warranty 72
3 Copyright © Atlas Scientific LLC

EZO circuit dimensions
TM
13.97mm
(0.55”)
20.16mm
(0.79”)
10.8mm
5.8mm 8.38mm
1mm Ø (0.4”)
(0.22”) (0.32”)
Power consumption Absolute max ratings

LED MAX STANDBY SLEEP Parameter MIN TYP MAX
5V ON 13.5 mA 13.1 mA 0.66 mA Storage temperature

(EZO™ D.O.) -65 °C 125 °C
OFF 12.7 mA 12.7 mA
Operational temperature
-40 °C 25 °C 85 °C
3.3V ON 12.1 mA 12 mA 0.3 mA (EZO™ D.O.)
OFF 11.9 mA 11.9 mA VCC 3.3V 5V 5.5V

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Operating principle
The Atlas Scientific™ EZO™ Dissolved Oxygen circuit works with:
Optical probe Slow response, requires external power, expensive.
Polar Graphic probe Requires external power, output in μA.
Galvanic probe Requires no external power, output in mV.
A galvanic dissolved oxygen probe consists of a Polytetrafluoroethylene membrane, an

anode bathed in an electrolyte and a cathode. Oxygen molecules defuse through the
probes membrane at a constant rate (without the membrane the reaction happens too
quickly). Once the oxygen molecules have crossed the membrane they are reduced
at the cathode and a small voltage is produced. If no oxygen molecules are present, the
probe will output 0 mV. As the oxygen increases so does the mV output from the probe.
Each probe will output a different voltage in the presence of oxygen. The only thing that
is constant is that 0mV = 0 Oxygen. (A galvanic dissolved oxygen probe can also be used
to detect the Oxygen content in gases).
Membrane
Cathode (Pure silver rod)
Electrolyte
Anode (Zinc)
Flow Dependence
Stagnant water
Dissolved Oxygen
90%
Oxygen being
consumed by probe
One of the drawbacks from using a galvanic
60% probe is that it consumes a VERY small amount
of the oxygen it reads. Therefore, a small amount
of water movement is necessary to take accurate
readings. Approximately 60 ml/min.
20%
0 5 10 15 20 25 30
Time (sec)

Power and data isolation
The Atlas Scientific EZO™ Dissolved Oxygen circuit is a very sensitive device. This sensitivity
is what gives the Dissolved Oxygen circuit its accuracy. This also means that the Dissolved
Oxygen circuit is capable of reading micro-voltages that are bleeding into the water from
unnatural sources such as pumps, solenoid valves or other probes/sensors.
When electrical noise is interfering with the Dissolved Oxygen readings it is common to
see rapidly fluctuating readings or readings that are consistently off. To verify that
electrical noise is causing inaccurate readings, place the Dissolved Oxygen probe
in a cup of water by itself. The readings should stabilize quickly, confirming that electrical
noise was the issue.
When reading Dissolved Oxygen and Conductivity together, it is strongly

recommended that the EZO™ Dissolved Oxygen circuit is electrically isolated
from the EZO™ Conductivity circuit. A
1 5 10 15 20 25 30
A A
1 5 10 15 20 25 30
A
Correct Incorrect
B B B B
C C C C
D D D D
1 E 5 10 15 20 25 30 E 1 E 5 10 15 20 25 30 E
A A A A
Correct Incorrect
B B B B
C F C F C F C F
D G D G D G D G
E H E H E H E H
I I I I
J J J J
F 1 5 10 15 20 25 F 30 F 1 5 10 15 20 25 F 30
G G G G
H H H H
I 1 5 10 15 20 25 I 30 I 1 5 10 15 20 25 I 30
Basic EZO TM J
1
A
B
5 10 15 20 25 30
J A
B
J
1
A
B
5 10 15 20 25 30
J A
B
C C C C
Inline Voltage Isolator A

D
1 E 5 10 15 20 25 30
A
D
E
A
1
D
E 5 10 15 20 25 30
A
D
E
B B B B
C C C C
F F F F
D D D D
G G G G
E E E E
H H H H
I I I I
F J F J F J F J
1 5 10 15 20 25 30 1 5 10 15 20 25 30
G G G G
H H H H
I I I I
J J J J
1 5 10 15 20 25 30 1 5 10 15 20 25 30
Without isolation, Conductivity readings

will effect Dissolved Oxygen accuracy.

r 0.1
This schematic shows exactly how we isolate data and power using the ADM3260
and a few passive components. The ADM3260 can output isolated power up to 150 mW
and incorporates two bidirectional data channels.
This technology works by using tiny transformers to induce the voltage across an air gap.
PCB layout requires special attention for EMI/EMC and RF Control, having proper ground
planes and keeping the capacitors as close to the chip as possible are crucial for proper
performance. The two data channels have a 4.7kΩ pull up resistor on both the isolated
and non-isolated lines (R1, R2, R3, and R4) The output voltage is set using a voltage
divider (R5, R6, and R,7) this produces a voltage of 3.9V regardless of your input voltage.
Isolated ground is different from non-isolated ground, these two lines should not
be connected together.
VCC = 3.0v − 5.5v ISO-VCC
VCC
ADM3260
VCC VCC
ISO-VCC
VDDP VDDISO ISO-VCC
VIN VISO R5 R7
HDR_TOP R2 4.7K R1 4.7K
VCC
VSEL
1.5K 1.5K
HDR_BTM
RX/SCL SCL2 SCL1 VCC
TX/SDA
EN SDA2 SDA1 RX/SCL
TX/SDA
GND
VCC NC NC NC
GND
C1
GNDP
0.1uf R6
GNDP GNDISO
GND 1.5K
C4
GNDP GNDISO R4 4.7K
GNDP GNDISO C3 R3 4.7K ISO-GND

VCC 10uF
PDIS GNDISO
VCC R5 0.1uF ISO-VCC
R8 C2
D
1M
0.1uF
1M
G N-FET ISO-VCC
C6
S
10uF
C5
GND
Non-isolated Isolated
10uF
ISO-GND

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Correct wiring
Bread board USB
Bread board via USB Carrier board carrier board
1 5 10 15 20 1 5 10 15 20
A A A A
GND
TX
RX
VCC
B B B B PWR
C C C C RX
D D D D TX
E E E E
Atlas
F F F F Scientific
G G G G TM
H H H H
I I I I
J J J J
1 5 15 15 20 1 5 15 15 20
Part # COM-104
Part # Part #
ISCCB USB-ISO
Incorrect wiring
Extended leads Sloppy setup Perfboards or Protoboards *Embedded into your device
A B C D E F G H I J
1 1
5 5
10 10
15 15
20 20
25 25
30 30
35 35
40 40
45 45
50 50
NEVER
55 55
use Perfboards *Only after you are familar
or Protoboards with EZO TM circuits operation
60 60
A B C D E F G H I J

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Calibration theory
Simple calibration Advanced calibration
mode mode
The most important part of calibration is watching the

5.02 readings during the calibration process.
4.81
4.60 Unstabilized
4.46 It's easiest to calibrate the device in its default state
3.72
3.58
(UART mode, with continuous readings enabled).
3.58
3.58
Stabilized Switching the device to I2C mode after calibration will
3.58
3.58 not affect the stored calibration. If the device must be
3.58 calibrated in I2C mode be sure to continuously request
Send
readings so you can see the output from the probe.
The Atlas Scientific EZO™ Dissolved Oxygen circuit, has

a flexible calibration protocol, allowing for single point
or dual point (optional) calibration.
Calibrate first, compensate later.

Temperature, salinity and pressure compensation
values have no effect on calibration.

Single point calibration
Carefully pull off and discard the cap from the Dissolved Oxygen probe. Let the Dissolved
Oxygen probe sit, exposed to air untill the readings stabalize. (small movement from one
reading to the next is normal).
55−5−30
−30
30
sec
sec
sec
Do not unscrew!
Cap is only used to protect
the probe during shipping.
15.03
14.45 Unstabilized
14.08
13.95
13.95
13.95 Stabilized
13.95 Once the readings have stabilized,
cal issue the calibration command.
*OK "cal"
9.09
9.09
Send
After calibration is complete, you should see readings between 9.09 – 9.1X mg/L.
(only if temperature, salinity and pressure compensation are at default values)

Dual point calibration (optional)
Only perform this calibration if you require accurate readings below 1.0 mg/L
After you have calibrated the EZO™ Dissolved Oxygen circuit using the "Cal" command;
Place the probe into the Zero Dissolved Oxygen calibration solution and stir the probe
around to remove trapped air (which could cause readings to go high). Let the probe sit in
Zero D.O. calibration solution untill readings stabalize. (small movement from one reading
to the next is normal).
Trapped
Trapped
Trapped
airairair
0:30
0:30
0:30
−−1:30
−1:30
1:30
1.40
1.28 Unstabilized
1.00
0.96
0.96
0.96 Stabilized
0.96 Once the readings have stabilized,
cal,0 issue the calibration command.
*OK "cal,0"
0.00
0.00
Send

How to preserve the
Zero D.O. calibration solution
Oxygen is everywhere. The Zero D.O. calibration solution has been designed to
chemically absorb oxygen. Once the bottle has been opened the test solution has been
exposed to oxygen and will slowly stop working.
Only the gas.

(not the flame)
N2 N2
Void
space
N2 N2 O2 O2 Gas
Inside each bottle of the calibration solution is a small amount of nitrogen gas that helps
displace oxygen out of the bottle during the filling process. When the Dissolved Oxygen
probe is removed from the bottle, oxygen will enter the bottle and begin to dissolve into
the solution.
In order slow down this process, fill the void space of the bottle with any gas (other than
oxygen) to preserve the calibration solution. Gas from a lighter works great if other gases
are currently unubtainable.

Default state
UART mode
Baud 9,600
Readings continuous
Speed 1 reading per second
Temperature compensation 20 °C
Salinity compensation 0 (Fresh water)
Pressure compensation 101.3 kPa (Sea level)
LED on
1,000 ms
Green Cyan
Standby Taking reading Transmitting
Available data protocols
Default
Unavailable data protocols

SPI
Analog
RS-485
Mod Bus
4–20mA
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UART mode
Settings that are retained if power is cut Settings that are NOT retained if power is cut
Baud rate Find
Calibration Pressure compensation
Continuous mode Salinity compensation
Device name Sleep mode
Enable/disable parameters Temperature compensation
Enable/disable response codes
Hardware switch to I2C mode
LED control
Protocol lock
Software switch to I2C mode
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UART mode
8 data bits no parity
1 stop bit no flow control
Baud 300
1,200
2,400
9,600 default
19,200
38,400
57,600
115,200 TX RX
RX
Data in RX TX
TX TX RX
Data out
CPU
Vcc 3.3V – 5.5V
VCC
0V 0V
Data format
Reading D.O. Data type floating point
Units mg/L & (% sat) mg/L = 2
when enabled
Decimal places % sat = 1
Encoding ASCII Smallest string 4 characters
Format string (CSV string when
% sat is enabled)
Largest string 16 characters
Terminator carriage return
Receiving data from device
2 parts
ASCII data string Carriage return <cr>

Command Terminator
9,600 baud
(default)
TX RX
CPU
7.82 <cr>
Receiver
Sender
Advanced
ASCII: 7 . 8 2 <cr>
Hex: 37 2E 38 32 0D
Dec: 55 46 56 50 13
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)

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Sending commands to device
2 parts
Command (not case sensitive) Carriage return <cr>

ASCII data string Terminator
TX RX
CPU
Sleep <cr>
Sender
Receiver
Short
Advanced
ASCII: S l e e p <cr>
Hex: 53 6C 65 65 70 0D
Dec: 83 108 101 101 112 13
Short
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LED color definition
Green Cyan Purple Red White

UART standby Taking reading Changing Command Find
baud rate not understood
LED ON
5V +0.4 mA
3.3V +0.2 mA

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UART mode
command quick reference
All commands are ASCII strings or single ASCII characters.
Command Function Default state
Baud change baud rate pg. 37 9,600
C enable/disable continuous reading pg. 23 enabled
Cal performs calibration pg. 25 n/a
Export export calibration pg. 26 n/a
Factory enable factory reset pg. 39 n/a
Find finds device with blinking white LED pg. 22 n/a
i device information pg. 33 n/a
I2C change to I2C mode pg. 40 not set
Import import calibration pg. 27 n/a
L enable/disable LED pg. 21 enabled
Name set/show name of device pg. 32 not set
O enable/disable parameters pg. 31 mg/L
P pressure compensation pg. 30 101.3 kPa
Plock enable/disable protocol lock pg. 38 disabled
R returns a single reading pg. 24 n/a
S salinity compensation pg. 29 n/a
Sleep enter sleep mode/low power pg. 36 n/a
Status retrieve status information pg. 35 n/a
T temperature compensation pg. 28 20˚C
*OK enable/disable response codes pg. 34 enable

LED control
Command syntax
L,1 <cr> LED on default
L,0 <cr> LED off
L,? <cr> LED state on/off?
Example Response
L,1 <cr> *OK <cr>
L,0 <cr> *OK <cr>
L,? <cr> ?L,1 <cr> or ?L,0 <cr>

*OK <cr>
L,1 L,0

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Find
Command syntax This command will disable continuous mode
Send any character or command to terminate find.
Find <cr> LED rapidly blinks white, used to help find device
Example Response
Find <cr> *OK <cr>
Baud rate Find
Calibration Pressure compensation
Continuous mode Salinity compensation
Device name Sleep mode
Enable/disable parameters Temperature compensation
Enable/disable response codes
Hardware switch to I2C mode
LED control
Protocol lock
Software switch to I2C mode
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Continuous reading mode
Command syntax
C,1 <cr> enable continuous readings once per second default
C,n <cr> continuous readings every n seconds (n = 2 to 99 sec)
C,0 <cr> disable continuous readings
C,? <cr> continuous reading mode on/off?
Example Response
C,1 <cr> *OK <cr>
DO (1 sec) <cr>
DO (2 sec) <cr>
DO (3 sec) <cr>
C,30 <cr> *OK <cr>

DO (30 sec) <cr>
DO (60 sec) <cr>
DO (90 sec) <cr>
C,0 <cr> *OK <cr>
C,? <cr> ?C,1 <cr> or ?C,0 <cr> or ?C,30 <cr>

*OK <cr>

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Single reading mode
Command syntax
R <cr> takes single reading
Example Response
R <cr> 7.82 <cr>
*OK <cr>
1,000 ms
Green Cyan
Standby Taking reading Transmitting
600 ms

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Calibration
Command syntax The EZOTM Dissolved Oxygen circuit
uses single and/or two point calibration
Cal <cr> calibrate to atmospheric oxygen levels

Cal,0 <cr> calibrate device to 0 dissolved oxygen
Cal,clear <cr> delete calibration data
Cal,? <cr> device calibrated?
Example Response
Cal <cr> *OK <cr>
Cal,0 <cr> *OK <cr>
Cal,clear <cr> *OK <cr>
Cal,? <cr> ?Cal,0 <cr> or ?Cal,1 <cr> or ?Cal,2 <cr>

single point two point
*OK <cr>
Cal <cr>
9.53 mg/L 8.82 mg/L

Export calibration
Command syntax Export: Use this command to download calibration settings
Export,? <cr> calibration string info

Export <cr> export calibration string from calibrated device
Example Response
Export,? <cr> 10,120 <cr> Response breakdown
10, 120
# of strings to export # of bytes to export
Export strings can be up to 12 characters long,

and is always followed by <cr>
Export <cr> 59 6F 75 20 61 72 <cr> (1 of 10)

Export <cr> 65 20 61 20 63 6F <cr> (2 of 10)
(7 more)
Export <cr> 6F 6C 20 67 75 79 <cr> (10 of 10)
Export <cr> *DONE Disabling *OK simplifies this process
Export <cr>
1
2 TX RX
3
4
MCU
5
6
7
8
9
10
[10,120]
*DONE
r 0.2
1
Import calibration
Command syntax Import: Use this command to upload calibration
settings to one or more devices.
Import,n <cr> import calibration string to new device
Example Response
Import, 59 6F 75 20 61 72 <cr> (1 of 10) *OK <cr>
1
Import, 65 20 61 20 63 6F 2
3
<cr> (2 of 10) *OK <cr>
TX RX
MCU
5
6
7
8
Import, 6F 6C 20 67 75 79 9
10
<cr> (10 of 10) *OK <cr>
[10,120]
Import,n <cr>
1
2 TX 1
RX
3
4
TX RX 2
MCU 3
5
6
7 4
MCU
8
9
5
10
6
[10,120]
7
8
9
10
[10,120]
1
TX RX 2
3
4
MCU
5
6
7
*OK <cr>
8
9
10
[10,120]
system will reboot
1
TX RX 2
* If one of the imported strings is not

1
3
TX RX 2
4
MCU
3
correctly entered, the device will not

4 5
MCU
5
6 6
7
7
8
9
10
8 accept the import, respond with *ER
9
and reboot.
[10,120]
10
[10,120]
*ER <cr>

r 0.2
Temperature compensation
Default temperature = 20°C
Command syntax Temperature is always in Celsius
Temperature is not retained if power is cut
T,n <cr> n = any value; floating point or int

T,? <cr> compensated temperature value?
RT,n <cr> set temperature compensation and take a reading*
This is a new command

for firmware V2.13
Example Response
T,19.5 <cr> *OK <cr>
RT,19.5 <cr> *OK <cr>

8.91 <cr>
T,? <cr> ?T,19.5 <cr>

*OK <cr>
T,19.5 <cr>
8.82 8.91

r 0.2
Salinity compensation
Default value = 0 μs
Command syntax If the conductivity of your water is less
than 2,500μS this command is irrelevant
S,n <cr> n = any value in microsiemens

S,n,ppt <cr> n = any value in ppt
S,? <cr> compensated salinity value?
Example Response
S,50000 <cr> *OK <cr>
S,37.5,ppt <cr> *OK <cr>
S,? <cr> ?S,50000,μS <cr> or ?S,37.5,ppt <cr>

*OK <cr>
S,50000 <cr>
8.91 mg/L 8.01 mg/L
29 Copyright © Atlas Scientific LLC SDA

(TX)
SCL
(RX)
SDA
(TX)
SC
(RX
Pressure compensation
Default value = 101.3 kPa
Command syntax This parameter can be omitted if the
water is less than 10 meters deep
P,n <cr> n = any value in kPa

P,? <cr> compensated pressure value?
Example Response
P,90.25 <cr> *OK <cr>
P,? <cr> ?,P,90.25 <cr>

*OK <cr>
P,90.25 <cr>
8.01 mg/L 6.94 mg/L

Enable/disable parameters
from output string
Command syntax
O, [parameter],[1,0] <cr> enable or disable output parameter
O,? <cr> enabled parameter?
Example Response
O,mg,1 / O,mg,0 <cr> *OK <cr> enable / disable mg/L
O,%,1 / O,%,0 <cr> *OK <cr> enable / disable percent saturation
O,? <cr> ?,O,%,mg <cr> if both are enabled
Parameters * If you disable all possible data types

mg mg/L your readings will display “no output”.
% percent saturation
Followed by 1 or 0
1 enabled
0 disabled

Naming device
Command syntax
Name,n <cr> set name n= ________________
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Name,? <cr> show name Up to 16 ASCII characters
Example Response
Name,zzt <cr> *OK <cr>
Name,? <cr> ?Name,zzt <cr>

*OK <cr>
Name,zzt Name,?
*OK <cr> Name,zzt <cr>

*OK <cr>

r 0.1
Device information
Command syntax
i <cr> device information
Example Response
i <cr> ?i,D.O.,1.98 <cr>
*OK <cr>
Response breakdown
?i, D.O., 1.98
Device Firmware

r 0.1
Response codes
Command syntax
*OK,1 <cr> enable response default
*OK,0 <cr> disable response
*OK,? <cr> response on/off?
Example Response
R <cr> 7.82 <cr>
*OK <cr>
*OK,0 <cr> no response, *OK disabled
R <cr> 7.82 <cr> *OK disabled
*OK,? <cr> ?*OK,1 <cr> or ?*OK,0 <cr>
Other response codes

*ER unknown command
*OV over volt (VCC>=5.5V)
*UV under volt (VCC<=3.1V) These response codes
*RS reset cannot be disabled
*RE boot up complete, ready
*SL entering sleep mode
*WA wake up

r 0.1
Reading device status
Command syntax
Status <cr> voltage at Vcc pin and reason for last restart
Example Response
Status <cr> ?Status,P,5.038 <cr>
*OK <cr>
Response breakdown
?Status, P, 5.038
Reason for restart Voltage at Vcc
Restart codes
P powered off
S software reset
B brown out
W watchdog
U unknown

r 0.1
Sleep mode/low power
Command syntax Send any character or
command to awaken device.
Sleep <cr> enter sleep mode/low power
Example Response
Sleep <cr> *OK <cr>
*SL <cr>
Any command *WA <cr> wakes up device
STANDBY SLEEP
5V 13.1 mA 0.66 mA
3.3V 12 mA 0.3 mA
Sleep <cr>
Standby Sleep
13.1 mA 0.66 mA

r 0.2
Change baud rate TX RX
RX TX
Command syntax
Baud,n <cr> change baud rate TX RX
Example Response CPU

Baud,38400 <cr> *OK <cr>
Baud,? <cr> ?Baud,38400 <cr>

*OK <cr>
300
1200
2400
n= 9600 default
19200
38400
57600
115200
Baud,38400 <cr> (reboot)
Standby Changing Standby

Green Cyan Purple
baud rate Red White
RT standby Taking reading Changing
*OK <cr> Command Find
baud rate not understood

r 0.1
Protocol lock TX RX TX RX
CPU
Command syntax
CPU
Locks device to UART mode.
Plock,1 <cr> enable Plock

Plock,0 <cr> disable Plock default
Plock,? <cr> Plock on/off?
Example Response
Plock,1 <cr> *OK <cr>
Plock,0 <cr> *OK <cr>
Plock,? <cr> ?Plock,1 <cr> or ?Plock,0 <cr>
Plock,1 I2C,100
Short
GreenRed
*OK <cr> Cyan
White
cannot change to I2C Purple
cannot change to IRed
C 2
*ER <cr>
UART Command
standby Find
Taking reading Changing Comma
e not understood baud rate not under
38 Copyright © Atlas Scientific LLC Short
r 0.1
Factory reset
Clears calibration
Command syntax LED on
"*OK" enabled
Factory <cr> enable factory reset
Example Response
Factory <cr> *OK <cr>
Factory <cr>
(reboot)
*OK <cr> *RS <cr>

*RE <cr>
Baud rate will not change
39
1,000 ms
Copyright © Atlas Scientific LLC
1,000 ms r 0.1
Change to I2C mode
Command syntax Default I2C address 97 (0x61)
I2C,n <cr> sets I2C address and reboots into I2C mode
n = any number 1 – 127
Example Response
I2C,100 <cr> *OK (reboot in I2C mode)
Wrong example Response

I2C,139 <cr> n > 127 *ER <cr>
I2C,100
(reboot)
Green Blue
Green
*OK <cr> Green Cyan Cyan Purple Pur
now in I C mode 2
UART standby
UART standby
Taking reading Changing Chan
Taking reading
baud ratebaud
r 0.2
Manual switching to I2C
• Make sure Plock is set to 0
• Disconnect ground (power off)
• Disconnect TX and RX TX RX
• Connect TX to PGND
• Confirm RX is disconnected
•
•
Connect ground (power on)
Wait for LED to change from Green to Blue
CPU
• Reconnect all data and power
Manually switching to I2C will set the I2C address to 97 (0x61)
Example
Short
Wrong Example
Short
Disconnect RX line

r 0.1
I C mode
2
The I2C protocol is considerably more complex than the UART
(RS–232) protocol. Atlas Scientific assumes the embedded
systems engineer understands this protocol.
To set your EZOTM device into I2C mode click here
Calibration Find
Change I2C address Pressure compensation
Enable/disable parameters Salinity compensation
Hardware switch to UART mode Sleep mode
LED control Temperature compensation
Protocol lock
Software switch to UART mode
r 0.1
I2C mode
I2C address (0x01 – 0x7F)
97 (0x61) default
Vcc 3.3V – 5.5V
Clock speed 100 – 400 kHz
4.7k resistor
VCC may be needed VCC
SDA
SDA SDA
SCL
SCL SCL
SDA
(TX)
SCL
(RX)
VCC SCL SDA
0V 0V
CPU
SDA
SCL
VCC
0V 0V
Data format
Reading D.O. Data type floating point
mg/L = 2
Units mg/L & (% sat) Decimal places % sat = 1
when enabled
Encoding ASCII Smallest string 4 characters

Format string (CSV string when
% sat is enabled) Largest string 16 characters
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
43 Copyright © Atlas Scientific LLC SCL SDA

Sending commands to device C
SDA
5 parts
SCL
Start I2C address Write Command (not case sensitive) Stop
VCC
97 (0x61) ASCII command string
0V 0V
Example
Start 97 (0x61) Write Sleep Stop
I2C address Command
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL SDA
CPU
Advanced
Address bits The entire command as ASCII with all arguments
SDA
A6 A5 A4 A3 A2 A1 A0 W ACK First letter of command ACK Last letter of command ACK
SCL
Start W = low Stop

r 0.1
Requesting data from device
7 parts
Start I2C address Read Response code Data string Null Stop
97 (0x61) 1 byte "7.82" Terminator
(Dec 0)
SDA
(TX)
SCL
(RX)
SCL SDA
CPU
7.82
Advanced
All bytes
Address bits N bytes of data after data are Null R = High
SDA
A6 − A0 R ACK Response code ACK Data ACK Data N ACK Null ACK Null
SCL
Start NACK Stop
1 55 46 56 50 0 = 7.82
Dec Dec
ASCII

r 0.1
Response codes
After a command has been issued, a 1 byte response
code can be read in order to confirm that the command
was processed successfully.
Reading back the response code is completely optional,

and is not required for normal operation.
Send
command
SDA
(TX)
SCL
(RX)
CPU
Processing delay
Receiving
data
Example The response code will always be 254,

if you do not wait for the processing
I2C_start;
delay.
I2C_address;
I2C_write(EZO_command);
SDA SCL (TX) (RX)
I2C_stop; Response codes

SCL SDA
Single byte, not string

delay(300); Processing delay
CPU
255 no data to send
I2C_start; 254 still processing, not ready
I2C_address; 2 syntax error
Char[ ] = I2C_read;
1 successful request
I2C_stop;

r 0.2
LED color definition
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
Blue Green Purple Red White

I2C standby Taking reading Changing Command Find
I C address
2
not understood
LED ON
5V +0.4 mA
3.3V +0.2 mA

r 0.1
I2C mode
command quick reference
All commands are ASCII strings or single ASCII characters.
Command Function
Baud change back to UART mode pg. 65
Cal performs calibration pg. 52
Export export calibration pg. 53
Factory enable factory reset pg. 64
Find finds device with blinking white LED pg. 50
i device information pg. 59
I2C change I2C address pg. 63
Import import calibration pg. 54
L enable/disable LED pg. 49
O removing parameters pg. 58
P pressure compensation pg. 57
Plock enable/disable protocol lock pg. 62
R returns a single reading pg. 51
S salinity compensation pg. 56
Sleep enter sleep mode/low power pg. 61
Status retrieve status information pg. 60
T temperature compensation pg. 55

LED control
Command syntax 300ms processing delay
L,1 LED on default

L,0 LED off
L,? LED state on/off?
Example Response
L,1 1 0
Wait 300ms Dec Null
L,0 1 0
Wait 300ms Dec Null
L,? 1 ?L,1 0 or 1 ?L,0 0

Wait 300ms Dec ASCII Null Dec ASCII Null
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
L,1 L,0

r 0.1
Find 300ms processing delay
Command syntax This command will disable continuous mode

Send any character or command to terminate find.
Find LED rapidly blinks white, used to help find device
Example Response
Find 1 0
Wait 300ms Dec Null
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
Short
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)

r 0.1
Taking reading
R return 1 reading
Example Response
R 1 7.82 0
Wait 600ms Dec ASCII Null
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
Wait 600ms
Green Blue
Taking reading Transmitting Standby

r 0.1
Calibration
Cal calibrate to atmospheric oxygen levels

Cal,0 calibrate device to 0 dissolved oxygen
Cal,clear delete calibration data
The EZOTM Dissolved Oxygen circuit
Cal,? device calibrated? uses single and/or two point calibration
Example Response
Cal 1 0
Wait 1300ms Dec Null
Cal,0 1 0
Wait 1300ms Dec Null
Cal,clear 1 0
Wait 300ms Dec Null
Cal,? 1 ?Cal,0 0 or 1 ?Cal,1 0

Wait 300ms Dec ASCII Null Dec ASCII Null
or 1 ?Cal,2 0
Dec ASCII Null

Export calibration
300ms processing delay
Command syntax Export: Use this command to download calibration settings
Export,? calibration string info

Export export calibration string from calibrated device
Example Response
Export,? 1 10,120 0 Response breakdown

Wait 300ms Dec ASCII Null 10, 120
# of strings to export # of bytes to export
Export strings can be up to 12 characters long
Export 1 59 6F 75 20 61 72 0 (1 of 10)
Export 1 65 20 61 20 63 6F 0 (2 of 10)
(7 more)
Export 1 6F 6C 20 67 75 79 0 (10 of 10)

Export
1 *DONE 0

r 0.2
Import calibration
Command syntax Import: Use this command to upload calibration
settings to one or more devices.
Import,n import calibration string to new device
Example Response
Import, 59 6F 75 20 61 72 (1 of 10) 1 0
Wait 300ms Dec Null
Import, 65 20 61 20 63 6F SDA
(TX)
SCL
(RX)
1
2 (2 of 10) 1
SCL SDA 0
3 Wait 300ms Dec Null
4
MCU
5
6
7
Import, 6F 6C 20 67 75 79 0
8
10
9
(10 of 10) Wait 300ms
1
Dec Null
[10,120]
Import,n
1
SDA SCL 2
1
SCL SDA
SCL SDA
(TX) (RX)
3
2 SDA
(TX)
SCL
(RX)
4
MCU
5 3
6
7 4
MCU
8
5
9
10 6
[10,120]
7
8
9
10
SCL SDA
1
2 SDA
(TX)
SCL
(RX)
[10,120]
3
4
MCU
5
6
7
1 *Pending 0
8
9
10
[10,120] Dec ASCII Null
1 system will reboot

SCL SCA 2 SDA
(TX)
SCL
(RX)
3
1
* If one of the imported strings is not

SCL SCA 2 SDA4 SCL
(TX) (RX)
MCU
3
4 5
MCU
5
correctly entered, the device will not

6
6
7
7
8
accept the import and reboot.

9 8
10
9
[10,120]
10
[10,120]
reboot
r 0.2
Temperature compensation
Default temperature = 20°C
Command syntax Temperature is always in Celsius
Temperature is not retained if power is cut
T,n n = any value; floating point or int 300ms processing delay
T,? compensated temperature value?

RT,n set temperature compensation and take a reading*
This is a new command

for firmware V2.13
Example Response
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
T,19.5 1 0
Wait 300ms Dec Null
RT,19.5 1 8.91 0
Green Green
Wait 900ms Dec ASCII Null Blu
Taking reading
Taking reading Transmitting
Transmitting Stand
T,? 1 ?T,19.5 0
T,19.5
8.82 8.91

r 0.2
Salinity compensation
S,n n = any value in microsiemens default

S,n,ppt n = any value in ppt
S,? compensated salinity value?
Example Response
S,50000 1 0
Wait 300ms Dec Null
S,37.5,ppt 1 0
Wait 300ms Dec Null
S,? 1 ?S,50000,μS 0
or
1 ?S,37.5,ppt 0
Dec ASCII Null
If the conductivity of your water is less

than 2,500μS this command is irrelevant

Pressure compensation
Command syntax
Green Green
Bl
P,n n =Taking reading
Taking
any value reading
in kPa Transmitting
ThisTransmitting
parameter can be omitted if Stan
the water is less than 10 meters deep
P,? compensated pressure value?
Example Response
P,90.25 1 0
Wait 300ms Dec Null
P,? 1 ?,P,90.25 0
P,90.25
8.01 mg/L 6.94 mg/L

Enable/disable parameters
from output string
O, [parameter],[1,0] enable or disable output parameter

O,? enabled parameter?
Example Response
O,mg,1 / O,mg,0 1 0 enable / disable mg/L
Wait 300ms Dec Null
O,%,1 / O,%,0 1 0 enable / disable percent saturation

Wait 300ms Dec Null
O,? 1 ?,O,%,mg 0 if both are enabled

Parameters * If you disable all possible data types

mg mg/L your readings will display “no output”.
% percent saturation
Followed by 1 or 0
1 enabled
0 disabled

Device information
i device information
Example Response
i 1 ?i,D.O.,1.98 0
Response breakdown
?i, D.O., 1.98
Device Firmware

r 0.1
Reading device status
Status voltage at Vcc pin and reason for last restart
Example Response
Status 1 ?Status,P,5.038 0
Response breakdown
?Status, P, 5.038
Reason for restart Voltage at Vcc
Restart codes
P powered off
S software reset
B brown out
W watchdog
U unknown

r 0.1
Sleep mode/low power
Command syntax
Send any character or
Sleep enter sleep mode/low power command to awaken device.
Example Response
Do not read status byte after
Sleep no response issuing sleep command.
Any command wakes up device
STANDBY SLEEP
5V 13.1 mA 0.66 mA
3.3V 12 mA 0.3 mA
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
SDA
(RX)
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
Sleep
Standby Sleep

r 0.1
Protocol lock
Plock,1 enable Plock Locks device to I2C mode.
Plock,0 disable Plock default

Plock,? Plock on/off?
Example Response
Plock,1 1 0
Wait 300ms Dec Null
Plock,0 1 0
Wait 300ms Dec Null
Plock,? 1 ?Plock,1 0
Plock,1 Baud, 9600 Short

ShortShort
SDA
(TX)
SDA SCLSCL
(TX) (RX) (RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SDA SCLSCL
(TX) (RX) (RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SDA SCLSCL
(TX) (RX) (RX)
SDA
(TX)
SCL
(RX)
cannot change to UART cannot change to UART

r 0.1
I2C address change
I2C,n sets I2C address and reboots into I2C mode
Example Response
I2C,100 device reboot
Warning! n = any number 1 – 127

Changing the I2C address will prevent
communication between the circuit and
the CPU until your CPU is updated with
the new I2C address.
Default I2C address is 97 (0x61).
I2C,100
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
(reboot)

r 0.2
Factory reset
Command syntax Factory reset will not take the device out of I2C mode.
Factory enable factory reset I2C address will not change
Example Response
Factory device reboot
Clears calibration
LED on
Response codes enabled
Factory
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
(reboot)

r 0.1
Change to UART mode
Command syntax TX RX TX RX
Baud,n switch from I2C to UART

CPU
CPU
Example Response
Baud,9600 reboot in UART mode
300
1200
2400
n= 9600
19200
38400
57600
115200
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SC
(RX
Baud,9600 (reboot)
Changing to
Blue Blue GreenGreen PurplePurple
UART mode Green White
Blue Red Red
C standby
I2C standby
Taking Taking
readingreading
Changing I2C standby
Changing Command Taking readingFind
Command
I2C address not understood
I2C address not understood

r 0.1
Manual switching to UART SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
• Make sure Plock is set to 0

• Disconnect TX and RX
• Connect TX to PGND
• Confirm RX is disconnected
• Connect ground (power on)
• Wait for LED to change from Blue to Green
• Reconnect all data and power
Example
Short
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)
Wrong Example
Short
Disconnect RX line
SDA
(TX)
SCL
(RX)
SDA
(TX)
SCL
(RX)

r 0.1
EZO circuit footprint
TM
17.78mm
(0.7”)
2.54mm
(0.1”)
1 In your CAD software

place a 8 position header. 2 Place a 3 position header at both
top and bottom of the 8 position. 3 Delete the 8 position header. The two 3
position headers are now 17.78mm (0.7”)
apart from each other.
1.05mm Ø 17.78mm
(0.7”)

r 0.1
Datasheet change log
Datasheet V 5.3
Moved Default state to pg 13.
Datasheet V 5.2
Updated firmware changes on page 70.
Datasheet V 5.1
Revised response for the sleep command in UART mode on pg 36.
Datasheet V 5.0
Revised calibration theory on page 9, and added more information on the Export
calibration and Import calibration commands.
Datasheet V 4.9
Corrected temperature compensation typo on pages 26 & 52.
Datasheet V 4.8
Revised isolation schematic on pg. 10
Datasheet V 4.7
Added new command:
"RT,n" for Temperature compensation located on pages 26 (UART) & 52 (I2C).
Added firmware information to Firmware update list.

Datasheet V 4.6
Added more information about temperature compensation on pages 26 & 52.
Datasheet V 4.5
Changed "Max rate" to "Response time" on cover page.
Datasheet V 4.4
Removed note from certain commands about firmware version.
Datasheet V 4.3
Added information to calibration theory on pg 7.
Datasheet V 4.2
Revised definition of response codes on pg 44.
Datasheet V 4.1
Updated firmware changes on pg. 66.
Datasheet V 4.0
Revised Enable/disable parameters information on pages 29 (UART) & 55 (I2C).
Datasheet V 3.9
Revised information on cover page.
Datasheet V 3.8
Update firmware changes on pg. 66.
Datasheet V 3.7
Revised Plock pages to show default value.

Datasheet V 3.6
Added new commands:
"Find" pages 21 (UART) & 48 (I2C).
"Export/Import calibration" pages 25 (UART) & 51 (I2C).
Added new feature to continous mode "C,n" pg 22.
Datasheet V 3.5
Added accuracy range on cover page, and revised isolation info on pg. 10.
Datasheet V 3.4
Added manual switching to UART information on pg. 59.
Datasheet V 3.3
Updated firmware changes to refeclt V1.99 update.
Datasheet V 3.2
Revised entire datasheet.

Firmware updates
V1.1 – Initial release (Oct 30, 2014)
• Change output to mg/L, then percentage (was previously percentage, then mg/L).
V1.5 – Baud rate change (Nov 6, 2014)

• Change default baud rate to 9600
V1.6 – I2C bug (Dec 1, 2014)

• Fixed I2C bug where the circuit may inappropriately respond when other I2C devices
are connected.
V1.7 – Factory (April 14, 2015)

• Changed “X” command to “Factory”
V1.95 – Plock (March 31, 2016)

• Added protocol lock feature “Plock”
V1.96 – EEPROM (April 26, 2016)

• Fixed bug where EEPROM would get erased if the circuit lost power 900ms
into startup.
V1.97 – EEPROM (Oct 10, 2016)

• Fixed bug in the cal clear command, improves how it calculates the DO,
adds calibration saving and loading.
V1.98 – EEPROM (Nov 14, 2016)

• Updated firmware for new circuit design.
V1.99 – (Feb 2, 2017)

• Revised "O" command to accept mg.
V2.10 – (April 12, 2017)

• Added "Find" command.
• Added "Export/import" command.
• Modified continuous mode to be able to send readings every "n" seconds.
V2.11 – (Sept 28, 2017)

• Fixed bug where the temperature would default to 0 on startup.
V2.12 – (Dec 19, 2017)

• Improved accuracy of dissolved oxygen equations.
V2.13 – (July 16, 2018)

• Added “RT” command to Temperature compensation.
V2.14 – (June 7, 2019)

• Fixed bug where the output buffer overflows when the cal and cal,0 point are too
close together.
Warranty
Atlas Scientific™ Warranties the EZO™ class Dissolved Oxygen circuit to be free of defect
during the debugging phase of device implementation, or 30 days after receiving the
EZO™class Dissolved Oxygen circuit (which ever comes first).
The debugging phase

The debugging phase as defined by Atlas Scientific™ is the time period when the EZO™
class Dissolved Oxygen circuit is inserted into a bread board, or shield. If the EZO™ class
Dissolved Oxygen circuit is being debugged in a bread board, the bread board must be
devoid of other components. If the EZO™ class Dissolved Oxygen circuit is being connected
to a microcontroller, the microcontroller must be running code that has been designed to
drive the EZO™ class Dissolved Oxygen circuit exclusively and output the EZO™ class
Dissolved Oxygen circuit data as a serial string.
It is important for the embedded systems engineer to keep in mind that the following
activities will void the EZO™ class Dissolved Oxygen circuit warranty:
• Soldering any part of the EZO™ class Dissolved Oxygen circuit.
• Running any code, that does not exclusively drive the EZO™ class Dissolved Oxygen
circuit and output its data in a serial string.
• Embedding the EZO™ class Dissolved Oxygen circuit into a custom made device.
• Removing any potting compound.

r 0.1
Reasoning behind this warranty
Because Atlas Scientific™ does not sell consumer electronics; once the device has been em-
bedded into a custom made system, Atlas Scientific™ cannot possibly warranty the EZO™
class Dissolved Oxygen circuit, against the thousands of possible variables that may cause
the EZO™ class Dissolved Oxygen circuit to no longer function properly.
Please keep this in mind:

1. All Atlas Scientific™ devices have been designed to be embedded into a custom
made system by you, the embedded systems engineer.
2. All Atlas Scientific™ devices have been designed to run indefinitely without
failure in the field.
3. All Atlas Scientific™ devices can be soldered into place, however you do so at your
own risk.
Atlas Scientific™ is simply stating that once the device is being used in your application,
Atlas Scientific ™ can no longer take responsibility for the EZO ™ class Dissolved Ox-
ygen circuits continued operation. This is because that would be equivalent to Atlas Sci-
entific™ taking responsibility over the correct operation of your entire device.

r 0.1

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DO EZO Datasheet

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V 5.

Reads Dissolved Oxygen

Response time 1 reading per sec

Supported probes Any galvanic probe

Data protocol UART & I2C

Default I2C address 97 (0x61)

Operating voltage 3.3V − 5V

Data format ASCII

This device has been designed to be soldered and can be soldered

Get this device working in a Do not embed this device without

3 Copyright © Atlas Scientific LLC

Power consumption Absolute max ratings

5V ON 13.5 mA 13.1 mA 0.66 mA Storage temperature

OFF 11.9 mA 11.9 mA VCC 3.3V 5V 5.5V

4 Copyright © Atlas Scientific LLC

A galvanic dissolved oxygen probe consists of a Polytetrafluoroethylene membrane, an

Cathode (Pure silver rod)

5 Copyright © Atlas Scientific LLC

When reading Dissolved Oxygen and Conductivity together, it is strongly

Inline Voltage Isolator A

Without isolation, Conductivity readings

6 Copyright © Atlas Scientific LLC

VCC = 3.0v − 5.5v ISO-VCC

GNDP GNDISO C3 R3 4.7K ISO-GND

7 Copyright © Atlas Scientific LLC

8 Copyright © Atlas Scientific LLC

The most important part of calibration is watching the

The Atlas Scientific EZO™ Dissolved Oxygen circuit, has

Calibrate first, compensate later.

9 Copyright © Atlas Scientific LLC

10 Copyright © Atlas Scientific LLC

11 Copyright © Atlas Scientific LLC

Only the gas.

12 Copyright © Atlas Scientific LLC

Speed 1 reading per second

Salinity compensation 0 (Fresh water)

Pressure compensation 101.3 kPa (Sea level)

Unavailable data protocols

ASCII data string Carriage return <cr>

17 Copyright © Atlas Scientific LLC

Command (not case sensitive) Carriage return <cr>

Green Cyan Purple Red White

19 Copyright © Atlas Scientific LLC

Command Function Default state

Baud change baud rate pg. 37 9,600

C enable/disable continuous reading pg. 23 enabled

Cal performs calibration pg. 25 n/a

Export export calibration pg. 26 n/a

Factory enable factory reset pg. 39 n/a

Find finds device with blinking white LED pg. 22 n/a

i device information pg. 33 n/a

I2C change to I2C mode pg. 40 not set

Import import calibration pg. 27 n/a

L enable/disable LED pg. 21 enabled

Name set/show name of device pg. 32 not set

O enable/disable parameters pg. 31 mg/L

P pressure compensation pg. 30 101.3 kPa

Plock enable/disable protocol lock pg. 38 disabled

R returns a single reading pg. 24 n/a

S salinity compensation pg. 29 n/a

OK,0 <cr> no response, OK disabled

OK,? <cr> ?OK,1 <cr> or ?*OK,0 <cr>

OK <cr> RS <cr>