Instructions For Use

Allegra X-30 Series

Centrifuges

B01145AC
December 2015

Beckman Coulter, Inc.

250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Allegra X-30 Series
Centrifuges
PN B01145AC (December 2015)

© 2015 Beckman Coulter, Inc.

Beckman Coulter, the stylized logo, and Allegra are

trademarks of Beckman Coulter, Inc. and are registered
with the USPTO.

All other trademarks, service marks, products, or services

are trademarks or registered trademarks of their
respective holders.

Contact Us
If you have any questions, contact our Customer Support
Center.

• Worldwide, find us via our website at

www.beckmancoulter.com/customersupport/support.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.

Printed in U.S.A.
 Revision History
For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or
system help for your instrument.

Issue AC, 12/2015

Changes were made to: CHAPTER 1, CE Mark.

Note: Changes or additions that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.

B01145AC iii
 Revision History

iv B01145AC
 Contents

Revision History, iii

Safety, 1-1
Alerts for Danger, Warning, Caution, Important, and Note, 1-1
Safety During Installation and/or Maintenance, 1-2
Electrical Safety, 1-2
Safety Against Risk of Fire, 1-2
Mechanical Safety, 1-2
Chemical and Biological Safety, 1-3
CE Mark, 1-4
RoHS Notice, 1-4

Introduction, 2-1
Intended Use, 2-1
Certification, 2-1
Scope, 2-2
Typographic Conventions, 2-2
CFC-Free Centrifugation, 2-2
Radio Interference, 2-3
Canadian Regulations, 2-3
Recycling Label, 2-3
Nonrefrigerated Model Specifications, 2-4
Refrigerated Model Specifications, 2-5
Available Rotors, 2-6

Description, 3-1
Introduction, 3-1
Instrument Function and Safety Features, 3-1
Instrument Function, 3-1
Models, 3-2
Safety Features, 3-2
Name Rating Plate, 3-2

v
 Contents

Chassis, 3-2
Housing, 3-2
Door, 3-3
Rotor Chamber, 3-3
Drive, 3-3
Temperature Sensing and Control (refrigerated models only), 3-4
Controls and Indicators, 3-4
Power Switch, 3-4
Control Panel, 3-5
System Keys, 3-5
Program Keys, 3-6
Digital Displays, 3-7

Installation, 4-1
Introduction, 4-1
Installing the Instrument, 4-1
Transportation Safety Devices, 4-2
Electrical Requirements, 4-2
Test Run, 4-4

Operation, 5-1
Introduction, 5-1
Run Procedure, 5-2
Preparation and Loading, 5-2
Entering Run Parameters, 5-5
Selecting a Rotor Number, 5-6
Setting Run Speed, 5-6
Setting RPM, 5-7
Setting RCF, 5-7
Setting Run Time, 5-7
Setting Run Temperature (refrigerated models only), 5-8
Setting Acceleration Rate, 5-8
Setting Deceleration Rate, 5-9
Starting a Run, 5-9
Pulse Function, 5-10
Changing Parameters During a Run, 5-11
Stopping a Run, 5-11
Unloading, 5-12
Disabling the Audible Tones, 5-12
Summary of Run Procedures, 5-13

Troubleshooting, 6-1
Introduction, 6-1

vi
 Contents

User Messages, 6-1

Other Possible Problems, 6-3
Emergency Access, 6-4

Care and Maintenance, 7-1

Introduction, 7-1
Maintenance, 7-1
Preventive Maintenance, 7-1
Cleaning, 7-2
Tube Breakage, 7-3
Decontamination, 7-3
Sterilization and Disinfection, 7-3
Storage and Transport, 7-4
Storage, 7-4
Returning a Centrifuge, 7-4
Supply List, 7-5
Replacement Parts, 7-5
Other, 7-5

Beckman Coulter, Inc.

Allegra X-30 Series Centrifuge Warranty

vii
 Illustrations

Illustrations

3.1 Interior View of the Rotor Chamber, 3-3

3.2 The Power Switch, 3-4
3.3 System Panels, 3-5
3.4 System Keys, 3-5
3.5 Program Keys, 3-6
3.6 Digital Displays, 3-8
4.1 Dimensions of the Refrigerated and Nonrefrigerated
Centrifuges, 4-3
5.1 Tapered Sleeve Position, 5-2
5.2 Default Parameters, 5-6
6.1 Screw Locations, 6-4
6.2 Internal Access, 6-5

viii
 Tables

Tables

5.1 Acceleration/Deceleration Times (in minutes:seconds), 5-9

6.1 Error Message Chart, 6-2
6.2 Troubleshooting Chart, 6-3

ix
 Tables

x
 CHAPTER 1

Safety

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to
how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.

WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis.

CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis.

IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

B01145AC 1-1
 Safety
Safety During Installation and/or Maintenance

Safety During Installation and/or Maintenance

These centrifuges each weigh 48 kg/106 lb (nonrefrigerated model) or 78 kg/172 lb (refrigerated

model). DO NOT attempt to lift or move one of them without assistance from another person.

Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing its power plug from the
receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the
instrument.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors. Do not centrifuge such materials (chloroform or ethyl alcohol for example) in this
instrument nor handle or store them within the 30-cm (1-ft) clearance envelope surrounding the
centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge.
• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.

1-2 B01145AC
 Safety
Chemical and Biological Safety 1

• Do not lift or move the centrifuge while the rotor is turning.

• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments may be embedded in their surfaces.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During
operation you should come within the envelope only to adjust instrument controls, if necessary.
Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge.
Never lean on the centrifuge or place items on the centrifuge while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.
• Because spills may generate aerosols, observe proper safety precautions for aerosol
containment.
• Handle all infectious samples according to good laboratory practices and methods to prevent
the spread of disease. Ask your laboratory safety officer to advise you about the level of
containment required for your application and about the proper decontamination or
sterilization procedures to follow if fluids escape from containers. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization Laboratory
Biosafety Manual) are handled; materials of a higher group require more than one level of
protection. Because spills may generate aerosols, observe proper safety precautions for aerosol
containment.
• Other infectious samples must also be handled according to good laboratory procedures and
methods to prevent spread of disease.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.

B01145AC 1-3
 Safety
CE Mark

CE Mark

A “CE” mark indicates that a product has been assessed before being placed on the market, and has
been found to meet European Union safety, health, and/or environmental protection requirements.

RoHS Notice

These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label — This label indicates that the electronic information product contains
certain toxic or hazardous substances. The center number is the Environmentally Friendly Use
Period (EFUP) date, and indicates the number of calendar years the product can be in operation.
Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows
indicate the product is recyclable. The date code on the label or product indicates the date of
manufacture.

China RoHS Environmental Label — This label indicates that the electronic information product does
not contain any toxic or hazardous substances. The center “e” indicates the product is
environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date.
Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable.
The date code on the label or product indicates the date of manufacture.

1-4 B01145AC
 CHAPTER 2

Introduction

Intended Use

This Allegra X-30 centrifuge is intended for use as a general purpose laboratory instrument for the
separation of components through the use of relative centrifugal force. Applications may include
the separation of chemicals, industrial or environmental samples, and bodily fluids such as blood
and urine, either alone or after the addition of reagents or other additives.

This centrifuge should be operated by qualified personnel only.

Certification

To ensure full system quality, Beckman Coulter Allegra X-30 Series centrifuges have been
manufactured in a registered ISO 9001:2008 or ISO 13485:2003 facility. They have been designed and
tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of conformity and certificates of
compliance are available at www.beckmancoulter.com.

International symbols that may be displayed on the centrifuge are illustrated and described on the
inside of the back cover page.

B01145AC 2-1
 Introduction
Scope

Scope

This manual is designed to familiarize you with the Beckman Coulter Allegra X-30 Series benchtop
centrifuges, their functions, specifications, operation, and routine operator care and maintenance.

• The following introductory pages contain the instrument specifications, as well as space,
electrical, and temperature conditions required for optimal centrifuge performance. A list of
available rotors is also included.
• CHAPTER 3, Description provides a brief physical and functional description of the centrifuge
and the operating controls and indicators.
• CHAPTER 4, Installation contains instructions for installing and connecting the centrifuge.
• Procedures for operating the centrifuge are summarized in CHAPTER 5, Operation.
• CHAPTER 6, Troubleshooting lists possible error messages and/or malfunctions, together with
probable causes and corrective actions required.
• Procedures for operator care and maintenance are presented in CHAPTER 7, Care and
Maintenance, as well as a brief list of supplies and replacement parts.

We recommend that you read this entire manual, especially CHAPTER 1, Safety and all safety-
related information, before operating the centrifuge or performing instrument maintenance.

NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that intended for use
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.

• Key names (for example, START or ENTER) and display names (for example, TEMP°C or SPEED)
appear in bold type.
• Cursor keys, used to increment values up or down when setting parameters, are shown as up and
down arrows (▲ or ▼).

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra X-30 Series centrifuges.

2-2 B01145AC
 Introduction
Radio Interference 2

Radio Interference

This instrument has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area may cause interference, in which case the user
will be required to correct the interference at his or her own expense.

Canadian Regulations
This digital apparatus does not exceed the Class A limits for radio noise emissions from digital
apparatus as set out in the radio interference regulations of the Canadian Department of
Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

1. the device was put on the European market after August 13, 2005 and
2. the device is not to be disposed via the municipal waste collection system of any member state
of the European Union.

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

B01145AC 2-3
 Introduction
Nonrefrigerated Model Specifications

Nonrefrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Speed
Set speed to 16,000 rpm (in 100-rpm increments)
Speed control actual rotor speed, ±50 rpm of set speed
Speed display actual rotor speed in 100-rpm increments or in RCF (when selected)
Time
Set time to 9 hr 59 min or continuous (∞)
Time display time remaining in run (timed run ±1 minute) or ∞ and elapsed time
(continuous run)
Acceleration 10 acceleration profiles
Deceleration 10 deceleration profiles
Ambient temperature range 4 to 35°C
Humidity restrictions <80% (noncondensing)
Dimensions
Width 46 cm (18.1 in.)
Depth 55 cm (21.7 in.)
Height, door closed 35.5 cm (14.0 in.)
Height, door open 78.7 cm (31.0 in.)
Weight 48 kg (106 lb)
Clearances (sides) 7.6 cm (3.0 in.)
Electrical requirements
120-V instrument 120 VAC, 6 A, 60 Hz
100-V instrument 100 VAC, 7 A, 50–60 Hz
220–240-V instrument 220–240 VAC, 2.6 A, 50–60 Hz
Electrical supply Class I
Maximum heat dissipation into room 1638 Btu/h (0.48 kW)
under steady-state conditions
Noise level 0.91 m (3 ft) in front of ≤68 dBa
instrument (approx.)
Installation (overvoltage) category II
Pollution degree 2a
a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

2-4 B01145AC
 Introduction
Refrigerated Model Specifications 2

Refrigerated Model Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Speed
Set speed to 18,000 rpm (in 100-rpm increments)
Speed control actual rotor speed, ±50 rpm of set speed
Speed display actual rotor speed in 100-rpm increments or in RCF (when selected)
Time
Set time to 9 hr 59 min or continuous (∞)
Time display time remaining in run (timed run ±1 min accuracy) or ∞ and elapsed
time (continuous run)
Temperature
Set temperature –20 to +40°C (in 1°C increments)
Temperature control (after equilibration) ±2.5°C of set temperature
Temperature display (after equilibration) chamber temperature in 1°C increments
Operating range 2 to 40°Ca
Ambient temperature range 10 to 35°C
Acceleration 10 acceleration profiles
Deceleration 10 deceleration profiles
Ambient temperature range 10 to 35°C
Humidity restrictions <80% (noncondensing)
Dimensions
Width 46 cm (18.1 in.)
Depth 70.7 cm (27.8 in.)
Height, door closed 37 cm (14.6 in.)
Height, door open 81.3 cm (32.0 in.)
Weight 78 kg (172 lb)
Clearances (sides) 7.6 cm (3.0 in.)
Electrical requirements
120-V instrument 120 VAC, 11.5 A, 60 Hz
100-V instrument 100 VAC, 12.6 A, 50–60 Hz
220–240-V instrument 220–240 VAC, 6.2 A, 50–60 Hz
Electrical supply Class I
Maximum heat dissipation into room under 3311 Btu/h (0.97 kW)
steady-state conditions
Noise level 0.91 m (3 ft) in front of ≤68 dBa
instrument (approx.)
Installation (overvoltage) category II
Pollution degree 2b

B01145AC 2-5
 Introduction
Available Rotors

a. Temperature range depends on rotor in use and speed (see applicable rotor manual).
b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

Available Rotors

See the applicable rotor manual for information on rotor use, care and maintenance, and rotor
accessories.

Refrigerated Nonrefrigerated
Rotor Part
Max Max Max Number/
Max RCF Max RCF Capacity Rotor Manual
Rotor Profile Description RPM (´ g) RPM (× g) (mL) Number

F2402Ha 18,000 29,756 16,000 23,511 24 × 1.5/2.0 361171

Fixed Angle GS-TB-021
45° Angle
rmax = 82 mm

FX301.5 16,000 28,672 13,200 19,515 30 × 1.5/2.0 392274

Fixed Angle MMR-TB-002
45° Angle
rmax = 100 mm

F0630 18,000 28,305 16,000 22,364 6 × 30 361231

Fixed Angle GS-TB-014
30° Angle
rmax = 78 mm

F0850 11,400 13,682 9000 8528 8 × 50 346640

Fixed Angle GS-TB-003
25° Angle
rmax = 94 mm

F0685 10,000 10,864 8000 6953 6 × 85 364650

Fixed Angle GS-TB-008
25° Angle
rmax = 97 mm

F1010 18,000 27,579 16,000 21,791 10 × 10 361221

Fixed Angle GS-TB-007
35° Angle
rmax = 76 mm

C0650 (Conical) 10,000 10,304 9000 8346 6 × 50 364670

Fixed Angle GS-TB-009
25° Angle
rmax = 92 mm

2-6 B01145AC
 Introduction
Available Rotors 2

Refrigerated Nonrefrigerated
Rotor Part
Max Max Max Number/
Max RCF Max RCF Capacity Rotor Manual
Rotor Profile Description RPM (´ g) RPM (× g) (mL) Number

C1015 (Conical) 10,000 10,416 9000 8437 10 × 15 364680

Fixed Angle GS-TB-011
25° Angle
rmax = 93 mm

S2096 3000 1109 3000 1109 6 ea. 96 ´ 0.3 mL 361111

Microtiter 2 ea. 96 × 2 mL GS-TB-005
rmax = 110 mm

S6096 4700 2721 4700 2721 6 ea. 96 ´ 0.3 mL B01430

Microtiter 2 ea. 96 × 2 mL
rmax = 110 mm

SX4250 4500 3901 4200 3398 4 × 250 392243

Swinging GS22-TB-002
Bucket
rmax = 172 mm

SX4400a 4700 4255 4200 3398 4 × 400 B01425

Swinging
Bucket
rmax = 172 mm

SX241.5 14,000 16,244 12,500 12,950 24 × 1.5/2.0 392271

Swinging MMR-TB-003
Bucket
rmax = 74 mm

a. Certified for biocontainment by Porton Down U.K.

B01145AC 2-7
 Introduction
Available Rotors

2-8 B01145AC
 CHAPTER 3

Description

Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-30
Series centrifuges. The operating controls and indicators are also described; instructions for their use are
in CHAPTER 5, Operation. Chemical compatibilities of materials listed in this manual can be found in
Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Instrument Function and Safety Features

Instrument Function
The Beckman Coulter Allegra X-30 Series benchtop centrifuges generate centrifugal forces required
for a wide variety of applications. Together with any of several Beckman Coulter rotors designed
specifically for use in these centrifuges, the instrument applications include:
• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, receptor binding, and column centrifugations.
• Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-
culture cells, cloning and replicate studies, in-vitro cytoxicity studies, receptor binding, and
genetic engineering experimentation.
• Rapid sedimentation of protein precipitates, large particles, and cell debris.
• Binding studies and separation of whole blood.
• Cell isolation.

The centrifuges are microprocessor-controlled, providing interactive operation. The instrument

design features a brushless three-phase drive system, automatic rotor overspeed identification
system, and a choice of acceleration/deceleration rates. The refrigerated models also have
temperature control systems. User messages and a series of audible tones alert the operator to
conditions that may need attention. (Instructions for disabling the audible tones are in
CHAPTER 4, Installation.)

B01145AC 3-1
 Description
Name Rating Plate

Models
The centrifuge is available in both nonrefrigerated and refrigerated models. See Nonrefrigerated
Model Specifications or Refrigerated Model Specifications in the Introduction for the operating
differences between the two models. Unless indicated otherwise, information in this manual is the
same for both models.

Safety Features
Allegra X-30 Series centrifuges have been designed and tested to operate safely indoors at altitudes
up to 2000 m (6562 ft).

Instrument safety features include:

• The door has an electromechanical door-locking mechanism to prevent operator contact with
spinning rotors. When the door is closed it locks automatically. It can be unlocked only by
pressing the OPEN DOOR key, and opened only when the power is on and the rotor is at rest. Two
independent monitoring systems prevent the door from opening if the rotor is spinning.
• A steel barrier surrounds the rotor chamber to provide full operator protection.
• An overspeed system continuously monitors the rotor during centrifugation. The system
includes a magnetic sensor on the drive motor and magnets embedded in the rotors.
Throughout the run, checks are made to ensure that the rotor does not exceed set speed.
• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause
imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is
not resting on a level surface.
• The centrifuge feet, made of rubber, have been designed to minimize possible rotation in the
event of a rotor mishap.

Name Rating Plate

The name rating plate is affixed to the rear of the centrifuge. Check that the line voltage agrees with
the voltage listed on this name rating plate before connecting the centrifuge. Always mention the
serial number and the model number shown when corresponding with Beckman Coulter regarding
your centrifuge.

Chassis

Housing
The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is
covered by a protective overlay made of coated polycarbonate.

3-2 B01145AC
 Description
Chassis 3

Door
The door is made of a solid sheet of steel, encased in foam molding. In the center of the door is a
window for strobe viewing. The door is secured to the housing by solid shafts. An electromechanical
door lock system prevents operator contact with spinning rotors and prevents run initiation unless
the door is shut and latched. The door is locked when a run is in progress and can be opened only
when the rotor is stopped. (A light-emitting diode [LED] on the OPEN DOOR key lights up when the
door can be opened.) In the event of a power failure, the door lock can be manually released for
sample recovery (see CHAPTER 6, Troubleshooting).

Rotor Chamber
The rotor chamber is shown in Figure 3.1. The drive shaft, mounting plate, rubber boot surrounding
the drive shaft, thermistor, and rotor detector are visible in the chamber bottom. A gasket system
around the chamber opening ensures sealing. (Instrument gaskets have not been designed as
bioseals for aerosol containment.)

Figure 3.1 Interior View of the Rotor Chamber

1
6

1. Drive Shaft 4. Gasket

2. Tapered Sleeve 5. Rotor Detector
3. Boot 6. Thermistor (refrigerated models only)

Drive
The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie-
down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads
are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs
during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast
processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

B01145AC 3-3
 Description
Temperature Sensing and Control (refrigerated models only)

Temperature Sensing and Control (refrigerated models only)

With the power on, the temperature control system is activated when the door is closed. The run
temperature can be set between –20 and +40°C in refrigerated models. If no set temperature is
entered, the centrifuge automatically selects the last entered temperature. (For the first run of a
new centrifuge, the instrument selects 20°C as its operating temperature.) A thermistor in the rotor
chamber continuously monitors chamber temperature. The micro-processor calculates the
required chamber temperature to maintain the selected rotor temperature.

NOTE In the unlikely event of a complete cooling system failure, the drive switches off if the chamber
temperature goes above 50°C. Restarting the centrifuge is not possible until the chamber is cooled.

Controls and Indicators

Power Switch
The power switch is located on the centrifuge right side panel (see Figure 3.2). This two-position
rocker switch (I, on; O, off) controls electrical power to the centrifuge.

Figure 3.2 The Power Switch

1. Power Switch

NOTE The power must be turned on before the chamber door can be opened.

3-4 B01145AC
 Description
Controls and Indicators 3

Control Panel
The control panel is mounted at an angle on the front of the centrifuge and includes system keys,
programming keys, and digital displays (see Figure 3.3). The panel also contains an IMBALANCE light
that flashes if rotor loads are severely out of balance.

Figure 3.3 System Panels

System Keys
The centrifuge operation is controlled through the system keys (see Figure 3.4). Each key (except
the PULSE key) has an LED in the upper left corner that lights to indicate that the key can be
activated.

Figure 3.4 System Keys

B01145AC 3-5
 Description
Controls and Indicators

START Pressing the START key causes the centrifuge run to begin. This key can also be used
to abort a deceleration process and restart the centrifuge.

STOP The STOP key can be pressed to end a run. It operates in two modes, depending on
how you press it:
• Normal stop (press and release):
The centrifuge decelerates to a complete stop according to the preselected
deceleration curve. Deceleration can be terminated and the centrifuge restarted by
pressing START again. The centrifuge emits a series of audible tones when the
rotor reaches 0 rpm. (Instructions for disabling the tones are in
CHAPTER 5, Operation.)
• Fast stop (press and hold for at least two seconds):
The centrifuge decelerates to a complete stop at the maximum rate. The
deceleration cannot be interrupted; the centrifuge can only be restarted after the
rotor stops and the door is opened and closed.

OPEN DOOR Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door
to be opened. The centrifuge accepts this command only when the rotor is completely
stopped and the OPEN DOOR key LED is lit.

PULSE Pressing the PULSE key causes the installed rotor to accelerate at the maximum rate
up to the set speed for short-duration runs (as long as the key is pressed).
Deceleration, at the maximum rate, begins when the key is released.

Program Keys
The program keys (see Figure 3.5) are used to set run parameters (a program consists of all of the
parameters for a run). Except for the cursor and ENTER keys, program keys are located beneath the
applicable digital displays, which show the parameters as they are input. Each key (except for the
cursor keys) has an LED in the upper left corner that lights to indicate operational readiness. The
LEDs also blink if an incorrect parameter is entered.

Figure 3.5 Program Keys

 The cursor keys are up and down arrow keys ( and ), which can be pressed to
(cursor keys) increment values up or down when setting parameters.

ENTER Parameter (speed, time, temperature, and acceleration or deceleration curve)

changes made while a run is in progress must be verified by pressing the ENTER key.

RPM When the RPM key is pressed the last digit in the SPEED display (0) flashes, indicating
that the speed can be entered in increments of 100 revolutions per minute (rpm).
After the run starts, the actual rpm of the rotor is displayed.

3-6 B01145AC
 Description
Controls and Indicators 3

RCF The RCF key can be used to select the speed setting by required relative centrifugal
field (RCF). The corresponding rpm is automatically calculated and displayed during
the run. If the RCF key is pressed during the run, the RCF value is shown in the SPEED
display.

ROTOR The centrifuge memory contains a list of the rotors that can be used, together with
default parameters for each rotor. When the ROTOR key is pressed, the number of
the rotor used in the previous run is shown on the SPEED display. The rotor list can
be scrolled through, using the cursor keys, until the required rotor number appears.

TIME The TIME key is used to select the run duration. When the TIME key is pressed, the
last digit on the TIME display flashes, indicating that the time can be entered with the
cursor keys.
• Timed run — Run time up to 9 hours and 59 minutes can be set. If the minutes
parameter exceeds 59, it is automatically converted into hours.
• Continuous run — If a run time of less than 1 minute or more than 9 hours and
59 minutes is selected, continuous operation is activated. Time is not counted
down, and the run continues until the STOP key is pressed.

TEMP The TEMP key is used to select run temperature on refrigerated model centrifuges.
(refrigerated When the TEMP key is pressed, the TEMP°C display flashes, indicating that the
models only) temperature can be entered with the cursor keys. Temperature can be set between
–20°C and +40°C. The operating temperature range is +2°C to +40°C, depending on
the rotor used and the speed selected.

ACCEL The ACCEL key is used to select acceleration rates that protect delicate gradients.
When the ACCEL key is pressed, the ACC/DEC display flashes, indicating that one of
ten preset rates can be entered with the cursor keys (9 is the fastest rate and 0 is the
slowest rate). Acceleration rates are described in Table 5.1 in CHAPTER 5, Operation.

DECEL The DECEL key is used to select deceleration rates that maintain optimum separation
while protecting delicate gradients. When the DECEL key is pressed, the ACC/DEC
display flashes, indicating that one of ten preset rates can be entered with the cursor
keys (9 is the fastest rate and 0 is a no-brake coast to stop). Deceleration rate
selections are described in Table 5.1 in CHAPTER 5, Operation.

Digital Displays
Digital displays indicate rotor speed, run time, rotor chamber temperature, and numbers that
represent selected acceleration and deceleration profiles (see Figure 3.6). When the power is turned
on, they show the operating parameters of the most recent run performed before the power was
turned off. The displays serve a dual purpose.

• When the run parameters are being set (the input mode), the displays show the set values
(those selected by the operator). When a run- parameter key (for example, TIME or RPM) is
pressed, the appropriate display flashes to indicate that data can be entered.
• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after
START is pressed.

NOTE Error messages (see Section 4) also appear on the displays, when applicable. The centrifuge emits a
series of audible tones to alert the user to an error condition.

B01145AC 3-7
 Description
Controls and Indicators

Figure 3.6 Digital Displays

RPM/RCF/ROTOR • In input mode, the RPM/RCF/ROTOR display shows the value of the parameter
being set, depending on the programming key pressed (RPM, RCF, or ROTOR).
For example, if the ROTOR programming key is pressed, a rotor number
appears on the SPEED display.
• During centrifugation, the SPEED display shows the speed of the rotor in rpm.
If the RCF key is pressed while the centrifuge is running, the RCF value is
displayed.

TIME • During a timed run (between 1 minute and 9 hours, 59 minutes), the TIME
display begins counting down when the rotor starts to spin and continues the
countdown until deceleration begins. The TIME display indicates the remaining
run time in hours and minutes.
• During a continuous run (less than 1 minute or more than 9 hours, 59 minutes
selected), countdown time is not displayed. Instead, the infinity (∞) symbol,
indicating continuous operation, lights up and the TIME display shows time
elapsed since the run start. After 9 hours and 59 minutes, the timer resets to 0
and continues counting elapsed time.

TEMP °C During standby (that is, the centrifuge is turned on but not spinning) and operation,
(refrigerated the TEMP °C display shows the actual temperature inside the rotor chamber (±2°C
models only) at an ambient temperature of 20°C).

ACCEL/DECEL The ACCEL/DECEL display shows the acceleration curve that was selected for the
run. The deceleration curve number can be displayed by pressing the DECEL key.

3-8 B01145AC
 CHAPTER 4

Installation

Introduction

This chapter contains instructions for installing and connecting the centrifuge. Check that required
clearances and electrical power are available.

WARNING
These centrifuges each weigh 48 kg/106 lb (nonrefrigerated model) or 78
kg/172 lb (refrigerated model.) DO NOT attempt to lift or move one of them
without assistance from a lifting device or another person.

Installing the Instrument

WARNING
Do not place the centrifuge near areas containing flammable reagents or
combustible fluids. Vapors from these materials could enter the centrifuge air
system and be ignited by the motor.

WARNING
Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it
is running. No persons should be within this clearance boundary while the
centrifuge is operating. Do not handle or store hazardous materials within the
30-cm (1-ft) area surrounding the centrifuge.

1 The centrifuge ships in a cardboard box on a wooden pallet. For easy access, remove the top of
the box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and
set them aside.
a. With the help of another person, move the centrifuge from the pallet to its final position.
• (Note the warning above regarding centrifuge weight.)

B01145AC 4-1
 Installation
Transportation Safety Devices

2 Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able
to support the weight of the centrifuge and resist vibration.
• Refer to Nonrefrigerated Model Specifications or Refrigerated Model Specifications in
CHAPTER 2, Introduction for weight.

a. Make sure that the centrifuge front feet are fully supported on the table.
b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation.
c. Check that there are 7.6-cm (3-in.) clearances at the sides of the centrifuge to ensure
sufficient air circulation.
Dimensions are shown in Figure 4.1. Additional clearance is required on the right side to
allow access to the power switch.
The centrifuge must have adequate air ventilation to ensure compliance to local
requirements for vapors produced during operation.
Ambient temperatures during operation should not be lower than 10°C (50°F) or higher
than 35°C (95°F) for refrigerated models, or lower than 4°C (39.2°F) or higher than 35°C
(95°F) for nonrefrigerated models. Relative humidity should not exceed 80%
(noncondensing).

NOTE During transport between areas with varying temperatures, condensation may occur inside
the centrifuge. Allow sufficient drying time before running the centrifuge.

Transportation Safety Devices

A sheet of foam rubber is installed in the rotor chamber at the factory. A hole in the center of the
foam stabilizes the drive shaft during transport. On receipt of the centrifuge, remove the foam and
store it in case future relocation of the centrifuge is necessary.

Electrical Requirements

See the Nonrefrigerated Model Specifications or Refrigerated Model Specifications in

CHAPTER 2, Introduction for centrifuge electrical requirements.

Make sure the voltage and frequency imprinted on the name rating plate affixed to the back of the
centrifuge agree with the line voltage and frequency of the outlet used. Refrigeration does not
function properly if the frequency [Hz] does not match the name rating plate. Plug in both ends of
the centrifuge power cord. If there is any question about voltage, have a qualified service person
measure it under load while the drive is operating.

A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. Make sure that the
matching wall outlet is located near the centrifuge and is easily accessible.

NOTE The power plug serves as the Disconnecting Device and must remain easily accessible.

4-2 B01145AC
 Installation
Electrical Requirements 4

Figure 4.1 Dimensions of the Refrigerated and Nonrefrigerated Centrifuges

NOTE Additional clearance is required on the right side to allow to allow access to the power switch.

B01145AC 4-3
 Installation
Test Run

WARNING
To reduce the risk of electrical shock, this equipment uses a three-wire electrical
cord and plug to connect the centrifuge to earth-ground. To preserve this safety
feature:
• Make sure that the matching wall outlet receptacle is properly wired and
earth-grounded. Check that the line voltage agrees with the voltage listed on
the name rating plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of
multiple-outlet receptacle strip.

Test Run

NOTE The centrifuge must be plugged in and the power switch turned to on position (I) before the door
can be opened.

We recommend that you make a test run to ensure that the centrifuge is in proper operating
condition following shipment. See CHAPTER 5, Operation for instructions on operating the
centrifuge.
After completing the test run, log on to www.beckmancoulter.com to register your centrifuge. This
validates the centrifuge warranty and ensures your receipt of further information regarding new
accessories and/or modifications as they become available.

4-4 B01145AC
 CHAPTER 5

Operation

Introduction

This section contains operating procedures for the centrifuge, using any of the Beckman Coulter rotors
designed for use in these centrifuges. Refer to the applicable rotor manual for instructions on preparing
the rotor for centrifugation. To prevent condensation, keep the centrifuge door closed and the power
turned off (O) when the centrifuge is not in use.

NOTE If the centrifuge is used in a manner other than that specified in this manual, the safety and
performance of this equipment could be impaired.

WARNING
Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Operator error or tube failure may generate
aerosols. Do not run potentially hazardous materials in this centrifuge unless all
appropriate safety precautions are taken. Always use the appropriate rotors and
adapters.

Handle all infectious samples according to good laboratory practices and methods
to prevent the spread of disease. Ask your laboratory safety officer to advise you
about the level of containment required for your application and about the proper
decontamination or sterilization procedures to follow if fluids escape from
containers. Biosafe containment should be used when Risk Group II materials (as
identified in the World Health Organization Laboratory Biosafety Manual) are
handled; materials of a higher group require more than one level of protection.
Because spills may generate aerosols, observe proper safety precautions
for aerosol containment.

WARNING
The centrifuge must not be used in the vicinity of flammable liquids or vapors, and
such materials should not be run in the centrifuge. During operation you should
come within the 7.6 cm (3-in.) clearance envelope only to adjust instrument
controls, if necessary. Never bring any flammable substances within the 30-cm
(1-ft) area surrounding the centrifuge. Do not lean on the centrifuge or place
items on the centrifuge while it is operating.

B01145AC 5-1
 Operation
Run Procedure

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you
are an experienced user of this centrifuge, you can turn to the summary for a quick review of
operating steps.

Preparation and Loading

For fast temperature equilibration, cool or warm the rotor to the required temperature before the run.

NOTE For high-speed runs at temperatures of 20°C or higher, prime the refrigeration system (refrigerated
models) by running the instrument at 10°C for 5 to 10 minutes beforehand to prevent overheating.

NOTE Before installing the rotor, lubricate it following the instructions in the rotor manual.

1 Check the name rating plate for the correct voltage, then plug the power cord into the wall
receptacle.

2 Press the power switch to on (I).

3 Press the OPEN DOOR key and lift the door up; it remains in the open position.

4 Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).
a. Remove the tie-down screw.

5 Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor (see Figure 5.2) and wipe the sleeve to be sure that it is clean and dry.
• The rotor rests on the sleeve while spinning, and does not operate properly if the sleeve is
missing.

Figure 5.1 Tapered Sleeve Position

1. Tapered Sleeve

NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service
representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States).

5-2 B01145AC
 Operation
Run Procedure 5

CAUTION
Do not drop the rotor onto the drive shaft. The shaft can be damaged if the rotor
is forced sideways or dropped onto it. Install the rotor by centering it over the
shaft and carefully lowering it straight down.

6 Install the rotor according to the instructions in the applicable rotor manual.
For a swinging bucket rotor, the steps include the following:
a. Lower the yoke straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

B01145AC 5-3
 Operation
Run Procedure

c. Seat the filled buckets onto the yoke pins.

• Be sure to fill all positions on the yoke with buckets.

For a fixed angle rotor, the steps include the following:

a. Lower the rotor straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

5-4 B01145AC
 Operation
Run Procedure 5

c. Attach the lid and tighten it. Some rotors have a knob and others have a bolt for the
T-handle wrench.

NOTE Fixed-angle rotors can be centrifuged without lids attached when longer tubes are used.

CAUTION
Always run any type of rotor with a balanced load.

7 Close the centrifuge door and push firmly down on both sides of the door front until you hear
a clicking (latching) sound.

8 Remove the rotor from the centrifuge if a long period between runs is anticipated.

CAUTION
If the rotor is left in the centrifuge between runs, make sure the rotor is seated on
the drive shaft and the tie-down screw is tight before each run.

Entering Run Parameters

When the power is applied for the initial use (no previous runs), default values are displayed (see
Figure 5.2). After the initial use, the parameters of the latest previous run are displayed when power
is applied.

When run parameters for a rotor have been entered, as described below, they are retained in the
centrifuge memory and can be recalled by simply entering the rotor number. The recalled program
can then be used for the current run or altered as required.

B01145AC 5-5
 Operation
Run Procedure

Figure 5.2 Default Parameters

1. Accelerate rotor to 2000 RPM.

2. Continue run for two minutes.
3. Cool chamber to 20°C (refrigerated models only).
4. Use acceleration curve 8.

Selecting a Rotor Number

Each rotor that can be run in the centrifuge has a rotor number; the number is engraved on the
rotor (and on the lid of fixed angle rotors). The centrifuge internal memory contains a list of all
rotors that can be run in the centrifuge, together with acceptable run parameters for each rotor.
If you enter an unauthorized rotor number then press the START key, an error message is displayed
and the run is aborted shortly after the rotor starts spinning.

1 Press the ROTOR key.

A rotor number (the last rotor previously centrifuged) appears on the SPEED display.

2 Press the ▲ or ▼ cursor key until the number of the rotor in use appears.

3 Press ENTER.
• Nominal parameter values (time, temperature, speed, acceleration and deceleration
curves) for the selected rotor are shown.
• You can use these parameters or set new values for the run.

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the selected rotor. Either
revolutions per minute (rpm) or relative centrifugal field (RCF) can be used to select speed. During
centrifugation, the SPEED display indicates the actual run speed (RPM) of the rotor.

5-6 B01145AC
 Operation
Run Procedure 5

Setting RPM

1 Press the RPM key.

• The last digit on the SPEED display (0) flashes, indicating that the RPM can be entered (in
100-RPM increments) with the cursor keys.

2 Press the ▲ or ▼ cursor key until the required RPM is displayed.

• The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is
displayed during the run.
• You can check the RCF during the run by pressing the RCF key while the centrifuge is
running.

Setting RCF

1 Press the RCF key.

• The last digit on the SPEED display (0) flashes, indicating that you can enter the RCF.

2 Press the ▲ or ▼ cursor key until the required RCF is displayed.

• The corresponding RPM is automatically calculated and the centrifuge runs at the
calculated speed.

Setting Run Time

Run time can be set for either a timed run or continuous operation.

Timed Run
Time can be set for up to 9 hours and 59 minutes (if the minutes parameter entered exceeds 59, it is
automatically converted into hours). During centrifugation, the TIME display begins counting down
when the rotor starts to spin and continues the count-down until deceleration begins. The TIME
display shows the time remaining in the run, in hours and minutes. When the time display reaches
zero, the run ends.

Continuous Run
If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected, continuous
operation is activated. Time is not counted down during continuous operation; instead, the infinity
(∞) symbol, indicating continuous operation, lights up and time elapsed since the run start is
displayed. The run continues until the STOP key is pressed.

1 Press the TIME key.

B01145AC 5-7
 Operation
Run Procedure

The last digit on the TIME display flashes, indicating that the time can be entered with the
cursor keys.

2 Press the    cursor key until the required run duration is displayed.

Setting Run Temperature (refrigerated models only)

Run temperature can be set between –20°C and +40°C. The typical operating range is from +2°C to
40°C, depending on the rotor and speed selected.

NOTE Temperatures may vary slightly between instruments. If sample temperature is crucial, test
temperature settings on your instrument using water samples.

1 Press the TEMP key.

The TEMP°C display flashes, indicating that the temperature can be entered with the cursor
keys.

2 Press the ▲ or ▼ cursor key until the required run temperature is displayed.

NOTE For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast
equilibration. For low-temperature runs, precool the centrifuge by running a 30-minute cycle at the
required temperature (with a precooled rotor installed) with the speed set at about 2000 rpm.

Setting Acceleration Rate

The ACCEL key is used to select acceleration rates that protect delicate gradients. When the ACCEL
key is pressed, the ACC/DEC display flashes, indicating that one of the 10 preset acceleration rates
can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration
(setting 9) can be used. However, if delicate gradients are being run, a lower setting may be needed.
Acceleration rates are listed in Table 5.1.

1 Press the ACCEL key.

• The ACC/DEC display flashes, indicating that the selected rate number can be entered with
the cursor keys.

2 Press the ▲ or ▼ cursor key until the required number is displayed.

5-8 B01145AC
 Operation
Run Procedure 5

Table 5.1 Acceleration/Deceleration Times (in minutes:secondsa)

Refrigerated Models Nonrefrigerated Models

Swinging Bucket Fixed Angle Swinging Bucket Fixed Angle

Rotors Rotors Rotors Rotors
(4 700 rpm)b (18,000 rpm) (4 200 rpm)b (16,000 rpm)

Curve Accel Decel Accel Decel Accel Decel Accel Decel

9 0:30 0:24 0:53 0:46 0:38 0:24 0:44 0:43

8 0:36 0:35 1:36 1:33 0:37 0:32 1:24 1:24
7 0:53 0:55 3:03 3:03 0:45 0:52 2:45 2:43
6 1:38 1:38 6:04 6:02 1:23 1:28 5:24 5:21

5 2:22 2:12 9:07 9:07 2:08 2:11 8:05 8:07

4 3:06 3:10 12:00 12:01 2:47 2:50 10:39 10:40
3 4:52 4:55 18:44 18:45 4:22 4:24 16:38 16:40

2 9:51 9:46 37:29 37:29 8:43 8:44 33:17 33:19

1 13:10 13:02 50:03 49:58 11:37 11:38 44:24 44:25
0 19:37 coastc 74:56 coastc 17:27 coastc 66:37 coastc
a. Times are approximate; actual times vary depending on the rotor in use, the rotor load, run speed, and voltage fluctuations.
b. Maximum speed for the S2096 microtiter rotor is 3000 rpm; acceleration and deceleration times decrease accordingly.
c. Deceleration setting 0 is a no-brake coast to stop and, in some cases, may take less time than deceleration setting 1.

Setting Deceleration Rate

The DECEL key is used to select deceleration rates that maintain optimum separation. When the
DECEL key is pressed, the ACC/DEC display flashes, indicating that one of the ten preset deceleration
rates can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum brake (setting 9)
can be used. However, if delicate gradients are being run, a lower brake setting may be needed.
Deceleration rates are listed in Table 5.1.

1 Press the DECEL key.

The ACC/DEC display flashes, indicating that the selected number can be entered with the
cursor keys.

2 Press the ▲ or ▼ cursor key until the required number is displayed.

Starting a Run
The run can be started using the parameters in memory from a previous run, or using new or
changed parameters that you enter using the procedure described above.

B01145AC 5-9
 Operation
Run Procedure

1 Check that all parameters are correct and the door is shut and latched.

2 Press the ENTER key, then the START key.

• As the run begins, the instrument rotor identification system compares the rotor in the
chamber to the entered rotor number and the speed entered.
• An incorrect rotor identification, or set speed greater than the rotor’s maximum
permitted speed, results in an error code and the centrifuge shuts down.
— See CHAPTER 6, Troubleshooting for information on error codes.
— The error must be cleared and an appropriate speed entered before the centrifuge
can be started.
— Throughout the run, checks are made to ensure that the rotor does not exceed
set speed.

• The SPEED display indicates the rotor speed in rpm.

— (The RCF can be checked by pressing the RCF key.)

• A blinking LED at the bottom of the TIME display indicates that the run is in progress.
— This display also shows the time remaining in the run (or ∞ and elapsed time for
continuous operation).

WARNING
Do not attempt to override the door interlock system while the rotor is spinning.

CAUTION
Do not lift or move the centrifuge while the rotor is spinning. Do not place items
on the centrifuge during operation.

Pulse Function
The pulse function, accessed by pressing the PULSE key, is used for short-duration runs as follows.

• When the PULSE key is pressed, the rotor accelerates at maximum rate to the set speed and
continues to spin as long as the PULSE key is pressed. (The current run time, acceleration, and
deceleration settings are overridden by the pulse function.) When the PULSE key is released, the
rotor begins decelerating to 0 rpm using maximum deceleration. Using the pulse function
eliminates the need to press the START and STOP keys.
• When the PULSE key is pressed, the TIME display begins displaying the elapsed seconds. When
the PULSE key is released, the seconds stop accumulating. The ACCEL/DECEL display continues
to show the user-entered settings during a pulse run even though the maximum rates are used.

5-10 B01145AC
 Operation
Run Procedure 5

• If the PULSE key is pressed while the rotor is running at set speed, the rotor continues running
at speed until the PULSE key is released. When the PULSE key is released, the rotor begins
decelerating to 0 rpm using maximum deceleration.
• The centrifuge memory retains the parameters of the last run performed before the PULSE key
was pressed. At the end of a pulse run, after the centrifuge door is opened and closed, the
previous run parameters are displayed.

Changing Parameters During a Run

While a run is in progress, run parameters (speed, time, temperature, and acceleration or
deceleration rate) can be altered without stopping the run. Run duration can also be changed from
continuous to a specified time period, or from a specified time period to continuous.

NOTE The deceleration rate cannot be changed after deceleration starts.

Use the program keys as described under Entering Run Parameters, above, to change parameters.
Parameter changes made during a run must be verified by pressing the ENTER key. For example, to
change run speed during centrifugation:

1 Press the RPM key.

• The last digit on the SPEED display flashes, indicating that the rpm can be raised or lowered
with the cursor keys.

2 Press the ▲ or ▼ cursor key until the required rpm is displayed.

3 Press the ENTER key.

• The current RPM value is displayed, changing to the new value as the rotor accelerates or
decelerates to the new speed selected.
— The corresponding RCF is automatically calculated by the centrifuge.

Stopping a Run
A timed run ends automatically when the TIME display counts down to zero. The centrifuge emits a
series of audible tones when the rotor reaches 0 rpm. (Instructions for disabling the audible tones
are on page 5-12, Disabling the Audible Tones.) To end a run in progress for any reason:

B01145AC 5-11
 Operation
Unloading

1 Press the STOP key for normal deceleration as selected by the deceleration curve.
(or)
Press and hold the STOP key for deceleration at the maximum rate (see Table 5.1).

NOTE If you hold the STOP key for more than a full second, the deceleration process cannot be
interrupted; the centrifuge cannot be restarted until the rotor comes to a complete stop and the door
is opened and closed.

2 After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to
release the door latches, then open the door.

NOTE To prevent chamber icing, use a sponge to wipe condensation out of the chamber bowl
between runs.

Unloading

NOTE When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft
does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter
Field Service (1-800-742-2345 in the United States).

After completing a run, unload the rotor following the instructions in the applicable rotor manual.

CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge and accessories.

Disabling the Audible Tones

The audible tones (beeps) that sound at the end of each run and if an error occurs may be turned on
and off by following the steps below (rotor must be at 0 rpm).

1 Press the STOP key and hold for 3 seconds until the word “Beep” appears in the display.

2 Press either the ▲ or ▼ cursor key to turn the beeps on or off.

• (The word “on” or “off” is displayed to indicate the current setting.
— The arrow keys toggle between on and off settings.)

5-12 B01145AC
 Operation
Summary of Run Procedures 5

3 Press ENTER to save the selection.

Summary of Run Procedures

For runs at other than room temperature, refrigerate or warm the rotor beforehand for fast equilibration.
For low-temperature runs, precool the centrifuge by running a 30-minute cycle (with a precooled rotor
installed) at the required temperature with the speed set at 2000 rpm.

1 Press the POWER switch to on (I).

a. Open the centrifuge door (press the OPEN DOOR key and lift the door up).

2 Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor.
• The rotor does not operate properly if the sleeve is missing.

3 Install the rotor according to the instructions in the applicable rotor manual.
a. Always run the rotor with a balanced load.

4 Close the centrifuge door and push firmly down on it until you hear the latch engage.

5 Enter run parameters:

a. Select a rotor number — ROTOR, ▲ or ▼, ENTER
b. Set run speed — RPM, ▲ or ▼; or RCF, ▲ or ▼
c. Set run duration — TIME, ▲ or ▼
d. Set run temperature — TEMP, ▲ or ▼
e. Select acceleration rate (0 through 9)— ACCEL, ▲ or ▼
f. Select deceleration rate (0 through 9)— DECEL, ▲ or ▼

6 Check that all parameters are correct and the door is shut and latched, then press ENTER,
then START.

WARNING
Never attempt to override the door interlock system while the rotor is spinning.

B01145AC 5-13
 Operation
Summary of Run Procedures

CAUTION
Do not lift or move the centrifuge while the rotor is spinning.

7 Wait for the set time to count down to zero, or end the run by pressing or holding the STOP key.

8 After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to
release the door latch; open the door.

9 Unload the rotor according to instructions in the applicable rotor manual.

CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures applied to the
centrifuge and accessories.

5-14 B01145AC
 CHAPTER 6

Troubleshooting

Introduction

This section lists possible malfunctions, together with probable causes and corrective actions
required. Maintenance procedures are contained in CHAPTER 7, Care and Maintenance. For any
problems not covered here, contact Beckman Coulter Field Service.

NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before
requesting service by Beckman Coulter Field Service.

User Messages

CAUTION
If the message SEr appears on the display, do not press any keys while the
message is displayed. Turn the centrifuge power off (O) and back on (I) to clear the
message. This message indicates that you have inadvertently accessed the
service mode. Pressing any keys while in this mode could erase the centrifuge
memory and critically interfere with future operation.

If a problem occurs during operation, the rotor decelerates to a stop, an error code appears on the
SPEED display, and the centrifuge emits a series of audible tones to alert the operator to the error
condition. Such problems may result from incorrect input or from an equipment malfunction. Refer
to Table 6.1 to determine the nature of the problem and recommended actions. If you are unable to
correct the problem, call Beckman Coulter Field Service. To help diagnose and correct the problem,
gather as much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (rotor in use, speed, load type, etc.).
• Note any unusual environmental and/or operating conditions (ambient temperature, voltage
fluctuations, etc.).
• Add any other information that may be helpful.

NOTE Instructions for disabling the audible tones are in CHAPTER 5, Operation.

B01145AC 6-1
 Troubleshooting
User Messages

Table 6.1 Error Message Charta

Error Number Problem Result Recommended Action

1 through 23, Microprocessor or Deceleration to full After rotor comes to a complete stop, turn
25 and 26, mechanical stop; run cannot be the power off (O), then back on (I) to reset.
28 through 30 malfunction restarted
32, 36, 39 and 40,
44 through 60,
63 through 68
23 and 24, 27, 31, Microprocessor or Deceleration to full After rotor comes to a complete stop, open
33 through 35, mechanical stop; run cannot be and close the centrifuge door, then restart.
37 and 38, malfunction restarted
41 through 43, 62
69 through 77 Microprocessor Run cannot start until Turn the power off (O), then back on (I) to
malfunction error is cleared reset.
78 through 80 Error during closing of Run cannot start 1. Remove debris in latch
door 2. Close lid quickly.
3. Turn the power off (O), then back on (I) to
reset.
81 “Door open” detected Maximum deceleration After rotor comes to a complete stop, shut
during run to full stop the centrifuge door, turn the power off (O),
then back on (I) to reset.
82, 83 Door does not open — See Emergency Access, below
84 Motor overtemperature Deceleration to full stop 1. Check that ambient temperature is
within a the limits shown in the
85 through 87 Rotor chamber Deceleration to full stop
Specifications in
overtemperature
CHAPTER 2, Introduction.
(refrigerated models)
2. Check air inlets and exhausts for
obstructions.
After the motor has cooled, restart. If the
problem persists, call Beckman Coulter Field
Service.b
90 through 96 Temperature sensor Maximum deceleration Turn the power off (O), then back on (I) to
malfunction to full stop reset.
(refrigerated models)
98 Rotor not recognized Maximum deceleration Check that the installed rotor is usable in the
to full stop centrifuge. If incorrect, install an authorized
rotor; if rotor is authorized and problem
persists, call Beckman Coulter Field Service.
99 Rotor recognized but Maximum deceleration Input correct parameters for the rotor in use,
incorrect to full stop then restart.
a. If the recommended action does not correct the problem, call Beckman Coulter Field Service.
b. In the United States, call 1-800-742-2345; outside the U.S., contact your local Beckman Coulter office.

6-2 B01145AC
 Troubleshooting
Other Possible Problems 6

Other Possible Problems

Possible malfunctions that may not be indicated by diagnostic messages are described in Table 6.2,
along with probable causes and corrective actions required. Possible causes for each problem are
listed in the probable order of occurrence. Perform the recommended corrective action in
sequence, as listed. If you are unable to correct the problem, call Beckman Coulter Field Service.

Table 6.2 Troubleshooting Chart

Problem Problem/Result Recommended Action

Imbalance LED lights 1. Rotor is out of balance 1. Check to be sure the rotor is in good condition and
and rotor decelerates is loaded symmetrically around the center of
to stop rotation, with containers of equal weight and
density opposite each other.
2. Centrifuge is misaligned (tilted) 2. Align the centrifuge on the bench or table.
3. Centrifuge was moved during 3. After the rotor comes to a complete stop, open and
operation close the centrifuge door, then restart.
4. Drive error (mechanical damage) 4. Call Beckman Coulter Field Service.

Rotor cannot achieve 1. Line voltage below rating 1. Have a qualified service person measure line
set speed voltage while the instrument is operating.
2. Electrical failure 2. Make sure both ends of the power cord are securely
connected; call Beckman Coulter Field Service.
3. Motor failure 3. Call Beckman Coulter Field Service.

Door does not open 1. Rotor spinning 1. Wait until the rotor stops.
2. Power not on 2. Plug in the power cord; turn power on (I).
3. Source power failure 3. See Emergency Access, below.
4. Latch stuck 4. See Emergency Access below.

Displays are blank 1. Power not on 1. Plug in the power cord; turn power on (I).
2. Electrical failure 2. Make sure both ends of the power cord are securely
connected; call Beckman Coulter Field Service.
3. Fuse blown 3. System fuses cannot be replaced by the user. Call
Beckman Coulter Field Service.

TEMP °C display Chamber temperature is >25°C Precool rotors before running at low temperatures.
flashes (refrigerated higher than selected temperature Precool rotor chamber by running a 30-minute cycle at
models) the desired temperature with the speed set at about
2000 rpm. If a lower temperature deviation alert is
required, contact Beckman Coulter Field Service.

Chamber does not Centrifuge cannot maintain selected Refer to applicable rotor manual for temperature and
reach selected temperature for rotor in use at speed speed requirements. Also, precool rotors before
temperature selected running at low temperatures. Precool rotor chamber by
(refrigerated models) running a 30-minute cycle at the desired temperature
with the speed set at 2000 rpm.

B01145AC 6-3
 Troubleshooting
Emergency Access

Emergency Access

If the facility power fails only momentarily, the centrifuge resumes operation when power is
restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have
to restart the run when the power is restored. In the event of an extended power failure, you may
have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

WARNING
Any maintenance procedure requiring removal of a panel exposes the operator to
the possibility of electrical shock and/or mechanical injury. Therefore, turn the
power off and disconnect the instrument from the main power source by removing
its power plug from the receptacle, and refer such maintenance to qualified
service personnel.

1 Turn the power switch to off (O) and disconnect the power cord from the main power source
by removing its power plug from the receptacle.

WARNING
Never attempt to override the door interlock system while the rotor is spinning.

2 Make sure that the rotor is not spinning.

CAUTION
Do not proceed if there is any sound or vibration coming from the drive.

3 Use a small flat-blade screwdriver to pry off the two round screw-hole covers on the centrifuge
front panel (see Figure 6.1).

Figure 6.1 Screw Locations

1. Upper screws and covers. 2. Lower screws, not visible from the front.

6-4 B01145AC
 Troubleshooting
Emergency Access 6

4 Set the covers aside.

5 Insert a Phillips-head screwdriver through one of the exposed holes in the centrifuge front
panel and loosen, but do not remove, the screw.
a. Repeat for the other screw.

6 Move the centrifuge forward on the bench until the two screws on the bottom of the centrifuge
are accessible (see Figure 6.1).

7 Use a Phillips-head screwdriver to remove the two screws and set them aside.

8 Carefully lift the centrifuge front cover until the bottom releases, then pull the top towards you
slightly.
To support the cover, insert the cover bottom lip under the standoff plate inside the centrifuge.

CAUTION
Do not allow the cover to hang down supported only by the exposed power and
grounding wires or the wires will be damaged.

9 Pull the latch-release cable firmly to the right until the latches release and the door opens
(see Figure 6.2).

Figure 6.2 Internal Access

1. Latch release cable.

2. Standoff plate.
3. Cover bottom lip goes here.

B01145AC 6-5
 Troubleshooting
Emergency Access

10 If the rotor is still spinning, close the door and wait until it stops before attempting to remove it.
WARNING
Never try to slow or stop the rotor by hand.

11 After removing the rotor, replace the front panel, screws, and covers.

6-6 B01145AC
 CHAPTER 7

Care and Maintenance

Introduction

For maintenance not covered in this manual, contact Beckman Coulter Field Service. User messages are
discussed in CHAPTER 6, Troubleshooting. Refer to the applicable rotor manual and to Chemical
Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before
requesting service by Beckman Coulter Field Service.

WARNING
Any maintenance procedure or servicing of this equipment that requires removal
of any covers can expose parts which involve the risk of electric shock or personal
injury. Make sure that the power switch is off (O) and the centrifuge is
disconnected from the main power source by removing its power plug from the
receptacle, and refer such servicing to qualified service personnel.

Do not use alcohol or other flammable substances in or near operating

centrifuges.

Maintenance

Preventive Maintenance
The following procedures should be performed regularly to ensure continued performance and
long service life of the centrifuge.

1 Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass
particles from broken sample tubes.
a. Clean as required (see Cleaning, below), as these accumulations can result in rotor
vibrations.

B01145AC 7-1
 Care and Maintenance
Cleaning

2 Regularly check the air intake and exhaust vents for obstructions.
a. Keep vents clear and clean.

3 Use a sponge to wipe condensation out of the chamber bowl between runs to prevent chamber
icing (refrigerated models).
a. If chamber icing occurs, defrost before use.

4 To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a
month, and after each cleaning.

Cleaning

Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when
they occur to prevent corrosives or contaminants from drying on component surfaces.

NOTE Before using any cleaning or decontamination methods except those recommended by the
manufacturer, users should check with the manufacturer that the proposed methods will not damage the
equipment.

1 To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes,
keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or
paper towel.

2 Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a
mild detergent such as Beckman Solution 555™ and a soft brush.
a. Dilute the detergent 10 to 1 with water.
b. Rinse thoroughly and dry completely.
c. Lubricate the drive shaft with Spinkote after cleaning.

3 Wash the bowl using a mild detergent such as diluted Solution 555.
a. Rinse thoroughly and dry completely.
b. If a cleaning solution other than Solution 555 is used, consult Chemical Resistances
(publication IN-175) or contact the cleaning-solution vendor to verify that the solution
will not damage the centrifuge.

4 Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555.

7-2 B01145AC
 Care and Maintenance
Tube Breakage 7

Do not use acetone or other solvents.

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, you must thoroughly
clean the interior of the chamber bowl.

WARNING
Be careful when examining or cleaning the sealing gasket or chamber, as sharp
glass fragments may be embedded in their surfaces.

1 Examine the gasket to make sure that no glass particles are retained in it.
a. Carefully remove any glass particles that may remain.

2 Carefully wipe away any glass particles that remain in the bowl.

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions,
perform appropriate decontamination procedures. Refer to Chemical Resistances (IN-175) to be sure
the decontamination method will not damage any part of the centrifuge.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%)* may be used on this surface. See
Chemical Resistances for more information regarding chemical resistance of centrifuge and
accessory materials.

While Beckman Coulter has tested these methods and found that they do not damage the
centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or
disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

* Flammability hazard. Do not use in or near operating centrifuges.

B01145AC 7-3
 Care and Maintenance
Storage and Transport

Storage and Transport

Storage
Before storing a centrifuge for an extended period, return it to its original shipping container
to protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge
installation) into the chamber, making sure the drive shaft is stabilized in the hole in the foam.
Temperature and humidity conditions for storage should meet the environmental requirements
described in the specifications given in CHAPTER 2, Introduction.

Returning a Centrifuge
Before returning a centrifuge or accessory for any reason, prior permission (a Returned Material
Authorization form for united States returns or Returned Goods Authorization form for
international returns) must be obtained from Beckman Coulter, Inc. Contact your local
Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts.
All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.

7-4 B01145AC
 Care and Maintenance
Supply List 7

Supply List

Contact Beckman Coulter Sales (worldwide offices are listed at the back of this manual or visit
www.beckman.com) for information about ordering parts, supplies, and publications. For your
convenience, a partial list is given below.

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts

Rotor tie-down screw 361367

T-handle wrench 361371

Other

Silicone vacuum grease (1 oz) 335148

Beckman Solution 555 (1 qt) 339555

B01145AC 7-5
 Care and Maintenance
Supply List

7-6 B01145AC
 Beckman Coulter, Inc.
Allegra X-30 Series Centrifuge Warranty
Subject to the exceptions and upon the conditions specified below, Beckman Coulter agrees to correct, either
by repair, or, at its election, by replacement, any defects of material or workmanship which develop within
one (1) year after delivery of the Allegra X-30 series centrifuge (the product), to the original Buyer by
Beckman Coulter or by an authorized representative provided that investigation and factory inspection by
Beckman Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one
(1) year. A complete list of such components or accessories is maintained at the factory and at each
Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to
be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable
period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What
constitutes either reasonable service and a reasonable period of time shall be determined solely by
Beckman Coulter.

Replacement
Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory,
transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless
the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions
Beckman Coulter shall be released from all obligations under all warranties either expressed or implied, if the
product(s) covered hereby are repaired or modified by persons other than its own authorized service
personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is
merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS
AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS
SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR
REPLACEMENT OF THE PRODUCT.

B01145AC Warranty-1
 Beckman Coulter, Inc. Allegra X-30 Series Centrifuge Warranty

Warranty-2 B01145AC
Symbol Simbolo
Symbol
Title / Titel / Titre / Titulo / Titolo / /
Symbole
Símbolo

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents

Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
! Attenzione: consultare le informazioni allegate

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)

Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra
 Related Documents
Additional References
• Chemical Resistances for Beckman Coulter
Centrifugation Products (IN-175)

Available in hard copy or electronic pdf by

request.

Available at www.beckmancoulter.com

www.beckmancoulter.com

© 2015 Beckman Coulter, Inc.

All Rights Reserved