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    The Microscope and Its Use: Lab Report

    Uploaded by

    Arbab Gill
    The document summarizes an experiment using compound microscopes to study specimens like onion cells and Spirogyra algae. Key details include: - The experiment aimed to study microscope operation and uses by examining specimens with objectives ranging from 4x to 160x magnification. - Measurements of field size and organism size under different magnifications were recorded, such as Spirogyra measuring 500 micrometers at 40x total magnification. - Proper microscope use and maintenance were discussed, noting that dirt or bubbles could obstruct clear viewing.

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    The Microscope and Its Use: Lab Report

    Uploaded by

    Arbab Gill
    879 views4 pages
    The document summarizes an experiment using compound microscopes to study specimens like onion cells and Spirogyra algae. Key details include: - The experiment aimed to study microscope operation and uses by examining specimens with objectives ranging from 4x to 160x magnification. - Measurements of field size and organism size under different magnifications were recorded, such as Spirogyra measuring 500 micrometers at 40x total magnification. - Proper microscope use and maintenance were discussed, noting that dirt or bubbles could obstruct clear viewing.

    Original Description:

    Principles of Biology lab report with essential parts

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    The document summarizes an experiment using compound microscopes to study specimens like onion cells and Spirogyra algae. Key details include: - The experiment aimed to study microscope operation and uses by examining specimens with objectives ranging from 4x to 160x magnification. - Measurements of field size and organism size under different magnifications were recorded, such as Spirogyra measuring 500 micrometers at 40x total magnification. - Proper microscope use and maintenance were discussed, noting that dirt or bubbles could obstruct clear viewing.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    879 views4 pages

    The Microscope and Its Use: Lab Report

    Uploaded by

    Arbab Gill
    The document summarizes an experiment using compound microscopes to study specimens like onion cells and Spirogyra algae. Key details include: - The experiment aimed to study microscope operation and uses by examining specimens with objectives ranging from 4x to 160x magnification. - Measurements of field size and organism size under different magnifications were recorded, such as Spirogyra measuring 500 micrometers at 40x total magnification. - Proper microscope use and maintenance were discussed, noting that dirt or bubbles could obstruct clear viewing.

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    © All Rights Reserved
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    You are on page 1of 4

    Experiment 2

    Title
    The Microscope and Its Use

    Aim
    To study the microscope, its operation and its uses

    Materials
    Compound microscope, stereo zoom microscope, microscope slides, spirogyra specimen,
    worms, onion, iodine solution, dropper, coverslip, testing needle, ruler and knife.

    Procedure
    Refer to laboratory manual page 9 to 16

    Results
    A. Identifying the Parts
    Interocular scale reading: 6 mm

    D. Computing Magnification
    Ocular Objective Magnification
    10x 4x 40x
    10x 40x 400x
    10x 100x 1000x
    10x 160x 1600x

    E. Measuring Objects in the Microscope


    1. Size of field under the lowest magnification
    Field = 6 mm
    Lowest total magnification = 40x

    2. Size of field under another magnification


    Field = 0.6 mm
    Total magnification = 400x
    Organism Spirogyra
    measured
    Objective lens 4x
    Total magnification 40x
    Size of field (m) 6000
    Relative size of 0.5
    organism (mm)
    Size of organism 500
    (m)

    Spirogyra under microscope:


    F. Preparing a Wet Mount
    Sketch:

    View:

    Total magnification = 100x


    Using the visual field as a guide, estimation the size of the organism: 125 m
    Organism identified: Onion cell

    Discussion
    A compound microscope was used throughout the experiment. A compound microscope has
    two lenses – the ocular lens and objective lens. The ocular lens is the one nearest to the eye
    and the objective lens is the one nearest to the specimen. There are four types of power of
    objective lenses which are 4x, 40x, 100x and 160x.
    A compound microscope is used to see living things that cannot be seen by the naked eye,
    such as spirogyra and onion cells. While observing the images, fine adjustment knob is used
    first followed by the coarse adjustment knob to ensure that the image is clear and focused at
    the centre.

    Possible errors that have occurred is that the lenses and the slides may have been dirty and
    thus the view of the specimen was not very clear. To improve these, the lens of the
    microscope and the specimen should be properly cleaned before using. Besides, precautions
    were also taken during the experiment. For instance, air bubbles were ensured not to be
    trapped between the cover slip and the specimen slide to ensure a clear viewing of the
    specimen through the microscope.

    Questions
    1. In which direction do you move the lever to increase the amount of light?
    The direction is clockwise.

    2. Do you turn the knob towards you or away from you?


    The knob is turned away from me.

    3. Does the object appear normal or upside down?


    The object appears upside down.

    4. Does it move away from you or towards you?


    The slide moves towards me.

    Conclusion
    I have learned how to properly and correctly use a microscope and the functions of the
    microscope as well. Besides, I’ve also learned to maintain the microscope at good condition
    and to appreciate it. When viewing a specimen through a microscope, the lowest power of
    objective lens has to be used, and only later gradually change the power of the objective lens
    to a higher power if needed for a more detailed viewing. I have also understood the sheer
    small size of cells and organisms through this experiment.

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