CV-Ankita Hiwale 2019

Ankita Arun Hiwale
Senior Research Fellow (CSIR-SRF)

Institute for Stem Cell Biology and Regenerative Medicine (Instem),
UAS-GKVK, Bellary road, Bangalore-560065, Karnataka, India.
Phone - 91+8095989358
Email- ankitaah10@gmail.com
Education-
1) Master of Science (MSc.)

Stem Cell Biology and Regenerative Medicine.
Manipal University, India.
(2013-2015)
2) Bachelor of Dental Surgery (BDS)

Maharashtra University of Health Sciences, India.
(2006-2011)
Research Experience-
1) Senior Research Fellow ( April 2018 – Present)

Dr. Praveen Kumar Vemula group
Institute for Stem Cell Biology and Regenerative Medicine (InStem), Bangalore, India
Project- Investigating efficacy of gut metabolites in reinforcing the endothelial junctions to
prevent or reduce vascular permeability in sepsis.
2) Junior Research Fellow (July 2015 – March 2018)

Institute for Stem Cell Biology and Regenerative Medicine (InStem), Bangalore, India.
Project- Developing hydrogel based delivery system for drugs and biologics
3) Project Trainee (January 2015- June 2015)
Institute of Stem Cell Biology and Regenerative Medicine (InStem), Bangalore, India.
Project- Effect of carbon chain length asymmetry of lipids on fusogenicity and transfection
efficiency of cationic liposomes.
4) Project Intern (May 2014- July 2014)
Dr. Ramesh Bhonde group
Manipal Institute of Regenerative Medicine (MIRM), Bangalore, India
Project- Selective cytokine profiling of dental pulp derived stem cells cultured under various
stress conditions.
5) Project Intern (August 2014- September 2014)
Dr. D. Anandh group
Manipal Institute of Regenerative Medicine (MIRM), Bangalore, India
Project- Effect of mesenchymal stem cells on mouse hippocampus treated with Kainic acid
Publications –
1) Enhancement of the gut barrier integrity by a microbial metabolite through Nrf2

pathway
Rajbir Singh , Dr. Sandeep Chandrashekharappa , Sobha Bodduluri , Becca Baby , Bindu
Hegde , Niranjan Kotla , Ankita A.Hiwale , Taslimarif Saiyed , Paresh Patel , Matam Vijay-
kumar , Dr. Morgan Langille , Mr. Gavin Douglas , Gerald Dryden , Houda Alatassi ,Huang-
Ge Zhang , Prof. Bodduluri Haribabu , Dr. Praveen Vemula – Nature Communications,
2019, 10, 89.
2) Prevention of pesticide-induced toxicity and mortality with nucleophilic poly-Oxime

topical gel
Ketan Thorat, Subhashini Pandey, Sandeep Chandrashekharappa, Nikitha V, Ankita A.
Hiwale, Purna Shah, Shubhangi Upadhyay, Sneha Sreekumar, Tenzin Phuntsok, Kiran
Kumar Mudnakudu-Nagaraju, Manohar Mahato, Omprakash Sunnapu, and Praveen Kumar
Vemula – Science Advances 2018, 4, eaau1780.
3) Scaling the effect of hydrophobic chain asymmetry on gene transfer properties of

cationic amphiphiles
Ankita A.Hiwale#, Chandrashekhar Voshavar#, Priya Dharmalingam, Ashish Dhayani,
Rajesh Mukthavaram, Rasajna Nadella, Omprakash Sunnapu, Sivaraman Gandhi, VGM
Naidu, Arabinda Chaudhuri, Srujan Marepally, Praveen Kumar Vemula - RSC Adv., 2017, 7,
25398-25405 (# equal contribution).
4) Asymmetric cationic lipids based non-viral vectors for an efficient nucleic acid delivery
Rakeshchandra R. Meka, Sudhakar Godeshal, Srujan Marepally, Ketan Thorat, Hari Krishna
Reddy Rachamalla, Ashish Dhayani, Ankita A.Hiwale , Rajkumar Banerjee, Arabinda
Chaudhuria,Praveen Kumar Vemula - RSC Adv., 2016, 6, 77841-77848.
Patents –
1) Jala VR, Bodduluri H, Singh R, Vemula PK, Chandrashekharappa S, Hiwale AA.

“Compounds, compositions, methods of using and methods for preparing comopunds”
International PCT Application: Application ref. number. 18034-03 (35783.04130) (Filed date:
14-05-2019)
2) Jala VR, Bodduluri H, Singh R, Vemula PK, Chandrashekharappa S, Hiwale AA. “Synthetic
analogs of gut microbial metabolites for protection of endothelial and epithelial barriers and
applications thereof” USPTO Number: 62/671,737 (Filed date: 15-05-2018)
Training –
High throughput drug screening, and RNAi based systemic gene silencing in Caenorhabditis
elegans.
Dr. Frederick Ausubel’s lab at Massachusetts General Hospital- Harvard Medical School,
Boston, USA. (June 2019 – August 2019)
Skills –
▪ Technical skills include –

1. Cell culture, maintenance and differentiation of primary cells and cell lines for in-vitro
assays.
2. Animal handling, injections and dissection for in-vivo experiments.
3. Handling, maintenance and performing high throughput drug screening assays on
Caenorhabditis elegans.
4. Molecular biology techniques- RNA, DNA and protein isolation and quantification, SDS
PAGE and WB, PCR, ELISA.
5. Handling phase contrast and fluorescent microscope, UV spectrophotometer, Microtome
and Cryotome for tissue sectioning.
6. Hydrogel preparation and cell encapsulation for 3D culture and maintenance.
7. Liposome preparation and cellular transfection for non-viral gene delivery.
8. Underwent training for FACS during masters.
▪ Project designing- Defining a problem by comprehending the current available
information and designing possible innovative solutions followed by its implementation.
▪ Data analysis with sound knowledge of MS office, GraphPad prism, ImageJ, and
inferring independent conclusions.
▪ Manuscript and proposal writing for publications.
▪ Organization and management by identifying goals and prioritizing tasks while
anticipating potential problems.
▪ Work effectively with limited supervision and under pressure to meet deadlines.
References –
Dr. Praveen Kumar Vemula

Institute for Stem Cell Biology and Regenerative Medicine (Instem)
National Center for Biological Sciences (NCBS), Bangalore, India
Email - praveenv@instem.res.in ; praveen.instem@gmail.com
Dr. Srujan Marepally
Center for Stem Cell Research
Christian Medical College, Vellore, India.
Email - srujankm@cmcvellore.ac.in ; srujan.marepally@gmail.com

CV-Ankita Hiwale 2019

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Ankita Arun Hiwale

Senior Research Fellow (CSIR-SRF)

1) Master of Science (MSc.)

2) Bachelor of Dental Surgery (BDS)

1) Senior Research Fellow ( April 2018 – Present)

2) Junior Research Fellow (July 2015 – March 2018)

1) Enhancement of the gut barrier integrity by a microbial metabolite through Nrf2

2) Prevention of pesticide-induced toxicity and mortality with nucleophilic poly-Oxime

3) Scaling the effect of hydrophobic chain asymmetry on gene transfer properties of

1) Jala VR, Bodduluri H, Singh R, Vemula PK, Chandrashekharappa S, Hiwale AA.

▪ Technical skills include –

Dr. Praveen Kumar Vemula

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