Lab 01 - Laboratory Safety

Laboratory #1
Safety
Laboratory #1
Safety
Skills=20
Objectives:
Upon completion of this unit, the student should be able to:
1. Describe and follow basic safety practices in the chemistry laboratory.

2. Demonstrate willingness and ability to search appropriate website(s) for additional information
on transmission-based precautions and / or other topics.
3. Outline and follow appropriate Standard Precautions at all times in Clinical Chemistry labs and
clinicals.
4. Recognize biohazard signs and risks and use the information to take steps in preventing the
spread of diseases transmissible by blood and body fluids.
5. Identify the hazards associated with chemical, biologic and radiological materials.
6. Identify the proper disposal of various wastes generated in the clinical lab.
7. Discuss the purpose of SDS (Safety Data Sheets) and list the types of information found on
SDS.
Materials:
1. Pen
2. Computer with internet access
Purpose:
The safety of students and workers in the laboratory is a major concern of regulation and accreditation
agencies as well as faculty and employers. If unchecked, potential hazards in the laboratory could
include biological, chemical, fire, radioactive, mechanical, and electrical hazards. The Occupational
Safety and Health Administration (OSHA) is responsible for providing standards for safety in the
workplace, including the laboratory setting. OSHA requires the availability of written safety manuals and
organized safety programs for employee within the workplace.
Procedure #1: Safety
1. During your tour of the MLAB / BITC laboratory and prep areas, note at least two (2)
chemicals with NFPA designation posted on them.
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MLAB 2401
Laboratory #1
Safety
2. List 2 (two) chemicals along with the NFPA hazard identification information (Health,
Flammable, Reactive, and other) and what each designation means for the specific chemical.
Place the information in the report sheet provided.
3. In the example below, the NFPA designation for chlorine bleach is shown. The “0” in the red
diamond means there is little chance of this chemical being flammable. The “3” in the yellow
diamond indicates an increased chance of instability of the chemical. The “2” in the blue
diamond indicates a health hazard of intermediate risk and the white diamond represents a
special hazard. In this example the words “OX” are in the white diamond to indicate an
oxidizer.
Procedure #2: SDS- Safety Data Sheet
1. Take one (1) of the chemicals (cannot use water) identified in Procedure 1 and locate an internet
SDS sheet for it. You can use Google or other search engines to locate free access to SDS
sheets. I found the VWR site friendly, it is at: http://tinyurl.com/q2fwq2d . The student will need
to print the SDS sheet to turn in.
2. Once the student has selected a chemical and printed out the SDS, underline or highlight the
required information as indicated on the report sheet. In the margin notate the number of the
category addressed. Be sure to write your name on the report form and indicate where it was
located or reference the link where the SDS was found.
Procedure #3: Biohazardous waste
1. Recognizing the appropriate waste receptacle is important for safety and economic reasons for
healthcare institutions.
2. The instructor will divide the class into small groups. Using the waste disposal guidelines below,
each student will sort cards with various waste products into the appropriate waste container.
Please have instructor verify and initial on the report sheet once the activity is complete.
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MLAB 2401
Laboratory #1
Safety
CARDBOARD OR PLASTIC SHARPS CONTAINERS
 Glass slides
 Graduated ESR Tubes
 Capillary tubes
 Wooden applicator sticks
 Any broken glass or anything that may potentially poke through a bag
 Analyzer probes
BIOHAZARD TRASH
 Blood samples
 Used vacuum collection tubes
 Bloody plastic pipettes
 Empty urine containers
 Pipettes used to test urine
REGULAR TRASH
 Gloves
 Items minimally contaminated with blood.
 Paper towels
 Kim wipes
 Lab mat
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MLAB 2401
Laboratory #1
Safety
Student Name:______________
Date:_________________
Lab #1: Safety

Report Form
Points= 20
Procedure #1: Safety

List two (2) chemicals along with the NFPA hazard identification information (Health, Flammable,
Reactive, and other) and what each designation means for the specific chemical. Place the information
in the table provided. ( 8 points)
Chemical DRAW NFPA Hazard Symbol Identify what each ID

designation means in respect to
this chemical.
1.
2.
Procedure #2: SDS

Select a chemical to look up SDS information. Using the table below as a guide highlight or
underline the required information on a printed copy of the SDS. Notate in the margin the
number the category addressed. Make sure to write your name on the printed SDS and notate
on the printout the source. (12 points)
1. Name of Substance:
* should also include alternative names
*name of manufacturer / shipper
*basic contact information where a knowledgeable person can be reached
2. Composition / Data on components:
this area should contain further information that identifies the chemical
3. Hazards Identification:
provide a brief summary of hazards associated with the use and exposure to the chemical
4. First aid measures:
first aid measures for anyone exposed to the material;
Skin contact:
Eye contact:
Ingestion:
5. Fire fighting measures:
provide brief summary of fire extinguishing media, special hazards presented &/ special protective
equipment
6. Accidental release measures:
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MLAB 2401
Laboratory #1
Safety
procedures to follow should the chemical be released into the environment;

Personal precautions:
Environmental precautions:
Methods for cleaning:
7. Handling and Storage:
ldentification of any technical measures / precautions needed to handle and store chemical or
materials
8. Exposure Controls:
should consider any
Engineering measures:
Control Parameters:
Personal protection equipment:
Eye protection:
Hand protection:
Hygiene measures:
9. Physical and chemical properties
should consider:
Appearance:
Odor:
pH:
Boiling point:
Melting point:
Flashpoint:
Explosive properties:
Vapor pressure:
Relative density:
Solubility:
10. Stability and reactivity
should consider:
Conditions to avoid:
Materials to avoid:
Hazardous decomposition products:
11. Toxicological information
should consider:
Acute toxicity:
Local effects
Excessive exposure may affect human health as follows:
Skin contact:
Eye contact:
Inhalation/ingestion:
12. Any additional information provided:
Ecological information
Disposal Considerations
Transport information
Classification data:
Regulatory information
Procedure #3: Biohazardous waste Instructors initials__________________

Have instructor initial once cards have been sorted in appropriate waste piles.
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MLAB 2401

Lab 01 - Laboratory Safety

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Laboratory #1

Upon completion of this unit, the student should be able to:

1. Describe and follow basic safety practices in the chemistry laboratory.

Procedure #1: Safety

Procedure #2: SDS- Safety Data Sheet

Procedure #3: Biohazardous waste

CARDBOARD OR PLASTIC SHARPS CONTAINERS

Lab #1: Safety

Procedure #1: Safety

Chemical DRAW NFPA Hazard Symbol Identify what each ID

Procedure #2: SDS

procedures to follow should the chemical be released into the environment;

Procedure #3: Biohazardous waste Instructors initials__________________

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